• 제목/요약/키워드: Negative Flow Control

검색결과 115건 처리시간 0.027초

신경 분화 유도한 인체 지방조직 유래 간질세포의 신경 표현형과 유전자 발현 (Neuronal Phenotypes and Gene Expression Profiles of the Human Adipose Tissue-Derived Stromal Cells in the Neuronal Induction)

  • 심수경;오득영;전영준;이백권;안상태;이종원
    • Archives of Plastic Surgery
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    • 제34권1호
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    • pp.1-7
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    • 2007
  • Purpose: Human adipose tissue-derived stromal cells(hADSCs) can be expanded in vitro and induced to differentiate into multiple mesenchymal cell types. In this study we have examined various neuronal phenotypes and gene expression profiles of the hADSCs in the neuronal induction. Methods: The hADSCs were isolated from human adipose tissue and they were characterized by the flow cytometry analysis using CD13, CD29, CD34, CD45, CD49d, CD90, CD105 and HLA-DR cell surface markers. We differentiated the hADSCs into the neuronal lineage by using chemical induction medium and observed the cells with contrast microscopy. The immunocytochemistry and western blotting were performed using the NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III antibodies. Results: The hADSCs were positive for CD13($90.3{\pm}4%$), CD29($98.9{\pm}0.7%$), CD49d($13.6{\pm}6%$), CD90 ($99.4{\pm}0.1%$), CD105($96%{\pm}2.8%$) but negative for CD34, CD45 and HLA-DR. The untreated cultures of hADSCs predominately consisted of spindle shaped cells and a few large, flat cells. Three hours after the addition of induction medium, the hADSCs had changed morphology and adopted neuronal-like phenotypes. The result of immunocytochemistry and western blotting showed that NSE, NeuN, Trk-A, Vimentin, N-CAM, S-100 and ${\beta}$-Tubulin III were expressed. However, NSE, NeuN, Vimentin were weakly expressed in the control. Conclusion: Theses results indicate that hADSCs have the capabillity of differentiating into neuronal lineage in a specialized culture medium. hADSCs may be useful in the treatment of a wide variety of neurological disorders.

긴구배수로 감세공의 Filp Bucket형 이용연구 (Experimental Study of Flip-Bucket Type Hydraulic Energy Dissipator on Steep slope Channel)

  • 김영배
    • 한국농공학회지
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    • 제13권1호
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    • pp.2206-2217
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    • 1971
  • 본연구(本硏究)는 Dam 또는 여수토(餘水吐) 방수로등(放水路等) 급구배수로(急勾配水路)에 고속(高速)으로 유하(流下)되는 물을 감세처리(減勢處理)하기 (爲)한 감세공형식중(減勢工型式中) 보다도 구조(構造)가 간단(簡單)하고 시공(施工)이 용역(容易)하며 경제성(經濟性)이 높은 Flip Bucket 형감세공(型減勢工)에 의(義)하여 수리특성(水理特性)에 따른 일반적(一般的) 적용조건(適用條件)과 설계시공(設計施工)의 발전(發展)을 도모(圖謀)하기 위(爲)하여 연구(硏究)한 것으로서 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. Flip Bucket의 수리특성(水理特性)과 일반적(一般的) 적용조건(適用條件) Flip Bucket는 일반적(一般的)으로 다음과 같은 조건(條件)을 갖일 때에 채용(採用)할 수 있다. 가. 하류하천(下流河川)의 수위(水位)가 얕어서 도수형(跳水型) 감세공법(減勢工法)을 이용(利用)하며는 막대(莫大)한 공사비(工事費)를 요(要)하게 될 때 나. 하류하천(下流河川)의 하상(河床)이 안정(安定)할 수 있는 양질(良質)의 암반(岩盤)일 경우 다. 하류하천(下流河川)은 여수토(餘水吐) 방수로(放水路)의 중심선(中心線)에 연(沿)하여 적어도 전수두(全水頭)의 $3{\sim}5$배(倍)되는 거리까지는 하심(河心)이 거이 직선(直線)인 여건(與件)에 있을 경우 라. 방사수맥(放射水脈)의 낙하지점(落下地點)을 중심(中心)으로 해서 주위(周圍)에 민가(民家), 경지(耕地), 중요시설물등(重要施設物等)이 없고 수맥낙하(水脈落下)로 인(因)하여 생기는 소음(騷音), 토사붕양(土砂崩壤), 물방울등(等)으로 피해(被害)를 받을 염려(念慮)가 없을 경우 2. 설계(設計) 및 시공상(施工上)의 적용사항(適用事項) 1항(項)과 같은 현지조건(現地條件)을 갖이고 실제(實際) Flip Bucket 형(型)으로 설계(設計) 또는 시공(施工)을 할 경우 고려(考慮)하여야 할 사항(事項)은 가. Bucket의 반경(半徑)(R)은 $R=7h_2$로 적용(適用)이 가능(可能)하다. ($h_2$: Bucket 시점(始點)의 평균수심(平均水深) 나. 본형식(本型式)은 한계지면이하(限界施面以下) 방수로(放水路)의 구배(勾配)가 $0.25<\frac{H}{L}<0.75$의 수로(水路)에서만 채용(採用)한다. 다. 방사수맥(放射水脈)은 가급적(可及的) 하상면(河床面)에 직각(直角)에 가까운 각도(角度)로 낙하(落下)시켜야 하며 그러기 위(爲)해서는 수맥(水脈)을 높이 또는 멀리 방사(放射)시켜야 한다. 상기목적(上記目的)을 만족(滿足)시키는 Flip의 앙각(仰角)은 $\theta=30^{\circ}{\sim}40^{\circ}$를 적용(適用)하는 것이 좋다. 라. 상기(上記) 가${\sim}$다항(項)을 적용(適用)했을 때 유량별(流量別) 방사수맥(放射水脈)의 낙하거리(落下距離)는 그림-4.1에 의(依)하여 쉽게 추정(推定)할 수 있다.(단 실물(實物)에 대(對)한 제량(諸量)의 환산(換算)은 표(表-3.2)에 제시(提示)된 Froude 상사율(相似律)을 적용(適用)할 것) 마. Bucket 부(部)에 Chute Blocks를 설치(設置)하는 것은 방사수맥(放射水脈)의 낙하범위(落下範圍)를 확장(擴張), Energy를 분배(分配)시켜 주므로 하류하상(下流河床)의 세굴심(洗掘深)을 감소(減少)시키는 이점(利點)은 있으나 소맥낙하거리(小脈落下距離)는 다소(多少) 단축(短縮)되는 경향(傾向)이 있다. 바. 수맥낙하점(水脈落下點)에는 세굴(洗掘)에 의(依)한 깊은 Water Cushion을 형성(形成)한다. 최종적(最終的)으로 도달(到達)하는 Water Cushion의 깊이는 하상구성재료(河床構成材料)의 조성(組成)과 재질(材質)에는 거이 무관(無關)하며 단위폭당(單位幅當)의 유량(流量)과 전수두(全水頭)에 따라 소요(所要) 깊이까지 세굴(洗掘)된다. 사. 빈도(頻度)가 잦은 소유량(小流量)에서는 수맥(水脈)의 낙하거리(落下距離)가 단축(短縮)되어 Flip Bucket 하류단(下流端) 직하류(直下流)를 세굴(洗掘)하게 되므 Bucket로 하류단(下流端)은 견고(堅固)한 암반(巖盤)에 충분(充分)한 깊이까지 삽입절연(揷入絶緣)시켜 수맥하부(水脈下部)의 공기유통(空氣流通)을 원활(圓滑)하게 하므로서 Cavitation을 방지(防止)할 수 있다. 지하벽(直下壁)은 보통(普通) Bucket 말단(末端)에서 약(約) $0.3{\sim}0.5m$ 정도(程度)는 수평(水平)으로 하고 수평(水平)과 내각(內角)이 $120^{\circ}{\sim}130^{\circ}$되게 절단(切斷)하여 적당(適當)한 곳에서 수직(垂直)으로 하여 암반(巖盤)에 견고(堅固)히 절연(絶緣)시킨다. 아. 하상(河床)에 돌입(突入)한 고속(高速) Jet는 수두(水頭)의 크기에 따라 막대(莫大)한 Energy의 일부(一部)를 함유(含有)한채 하상면상(河床面上)을 유하(流下)하게 되므로 이 영향(影響)을 받는 하류제방(下流堤防)에는 상당구간(相當區間)까지 사석(捨石) 또는 기타(其他)의 방호조치(防護措置)를 강구(講究)해야 한다. 자. 낙하지점(落下地點)의 조건(條件)으로 보아 자연낙하지점(自然落下地點)보다 더욱 양호(良好)한 지점(地點)이 주위(周圍)에 구비(具備)되어 있을 경우에는 별도(別途)로 수리실험(水理實驗)을 통(通)하여 수맥(水脈)의 변이방법(變移方法)을 강구(講究)해야 한다. 차. 수로(水路)의 중심선(中心線)이 만곡(灣曲)을 갖던가 또는 본연구(本硏究) 범위(範圍)에서 제외(除外)된 구조물(構造物)에서 본형식(本型式)을 계획(計劃)할 때는 별도(別途)로 수리실험(水理實驗)을 행(行)하여야 한다.

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강원도 춘천시 근교의 산지계류에 형성된 계단상 하상구조의 특징 (The Morphologic Characteristics of Step-pool Structures in a Steep Mountain Stream, Chuncheon, Gangwon-do)

  • 김석우;전근우;박종민;남수연;임영협;김영설
    • 한국산림과학회지
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    • 제100권2호
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    • pp.202-211
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    • 2011
  • 강원도 춘천시 근교의 강원대학교 산림환경과학대학 학술림 내의 산지계류를 대상으로 계단상 하상구조의 기하학적 특성과 이에 대한 계류 지형인자들과의 관계를 분석하였다. 조사구간에 형성된 스텝의 간격, 높이 및 낙차의 평균값은 각각 4.69 m, 0.47 m 및 0.71 m, 스텝의 구성입경은 평균 0.68 m, 스텝의 형성개수는 100 m당 평균 21 개인 것으로 나타났다. 스텝의 간격은 하폭대비 약 0.5로 선행연구들에 비해 작은 것으로 나타났으며, 스텝의 기울기는 평균 0.13이었다. 스텝의 각 기하학적 특성과 하폭과의 사이에는 모두 상관관계가 존재하지 않았으나, 스텝의 간격과 높이는 하상경사와 각각 부 정의 상관관계를 나타내었다. 또한 스텝의 기울기는 하상경사와 정의 상관을 보였고, 하상경사와의 비가 1.2로 나타나 유수에 대한 최대저항조건을 만족하며 비교적 안정상태를 유지하고 있는 것으로 나타났다. 특히 하상경사는 계단상 하상구조의 기하학적 형상(스텝의 낙차와 높이의 비)에 영향을 미치는 것으로 나타났다. 한편 스텝의 간격과 낙차는 거석의 입경과 모두 정의 상관관계를 보였다. 이러한 결과들로부터 계단상 하상구조의 동태는 산지계류의 물리 생태적 환경에 큰 영향을 미칠 것으로 판단되며, 산지가 대부분인 우리나라의 계류관리에 있어서 특히 중요하게 인식되어야 할 것이다.

산지계류에 있어서 유목의 종단적 분포특성 (Longitudinal Pattern of Large Wood Distribution in Mountain Streams)

  • 서정일;전근우;김민식;염규진;이진호;木村正信
    • 한국산림과학회지
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    • 제100권1호
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    • pp.52-61
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    • 2011
  • 수변역을 구성하는 중요한 요소인 유목은 유역의 생태적 환경에 영향을 미치는 동시에 재해의 요인으로서 인간생활권에 악영향을 미치기도 한다. 그럼에도 불구하고 우리나라에서는 유목의 발생, 이동 및 체류특성에 대한 구체적인 연구가 진행되고 있지 않다. 따라서 이 연구에서는 산지계류에 있어서 유목체류의 종단적 분포의 이질성에 영향을 미치는 인자를 규명하고, 그에 따른 유목체류량의 변화를 해석하기 위해 강원도 인제군에 위치한 기룡산 유역을 대상으로 유목의 종단적 분포특성을 파악하였다. 조사 결과, 조사구간의 평균계폭, 직경 1 m 이상의 거력의 개수 및 이들 간의 상호관계가 유목체류량을 설명하는 최적의 예측자로서 나타났다. 일반적으로 산지계류에 분포하고 있는 거력은 유목의 이동을 방해하는 장해물로서 유목체류에 적절한 물리적 환경을 조성한다. 그러나 이 연구에서는 이러한 거력의 영향이 산지계류의 규모에 따라 다르게 나타나는 것으로 나타났다. 즉 계폭이 좁은 구간의 경우 산지계류 내에 분포하는 거력의 개수가 증가함에 따라 유목체류량 역시 지속적으로 증가한 반면, 계폭이 큰 구간의 경우 유목체류량에 미치는 거력의 개수의 영향이 작은 것으로 나타났다. 이는 계폭이 다른 두 지역에 있어서 동일한 양의 거력들이 존재할 경우 이들에 의한 계폭의 감소비율이 다르기 때문인 것으로 사료된다. 따라서 이러한 유목의 종단적 분포특성 및 이에 영향을 미치는 생태적 물리적 환경을 고려한 계통적이고 체계적인 사방사업이 실시되어야 할 것이다.

한국전쟁의 교훈과 대비 -병력수(兵力數) 및 부대수(部隊數)를 중심으로- (The lesson From Korean War)

  • 윤일영
    • 안보군사학연구
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    • 통권8호
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    • pp.49-168
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    • 2010
  • Just before the Korean War, the total number of the North Korean troops was 198,380, while that of the ROK(Republic of Korea) army troops 105,752. That is, the total number of the ROK army troops at that time was 53.3% of the total number of the North Korean army. As of December 2008, the total number of the North Korean troops is estimated to be 1,190,000, while that of the ROK troops is 655,000, so the ROK army maintains 55.04% of the total number of the North Korean troops. If the ROK army continues to reduce its troops according to [Military Reform Plan 2020], the total number of its troops will be 517,000 m 2020. If North Korea maintains the current status(l,190,000 troops), the number of the ROK troops will be 43.4% of the North Korean army. In terms of units, just before the Korean War, the number of the ROK army divisions and regiments was 80% and 44.8% of North Korean army. As of December 2008, North Korea maintains 86 divisions and 69 regiments. Compared to the North Korean army, the ROK army maintains 46 Divisions (53.4% of North Korean army) and 15 regiments (21.3% of North Korean army). If the ROK army continue to reduce the military units according to [Military Reform Plan 2020], the number of ROK army divisions will be 28(13 Active Division, 4 Mobilization Divisions and 11 Local Reserve Divisions), while that of the North Korean army will be 86 in 2020. In that case, the number of divisions of the ROK army will be 32.5% of North Korean army. During the Korean war, North Korea suddenly invaded the Republic of Korea and occupied its capital 3 days after the war began. At that time, the ROK army maintained 80% of army divisions, compared to the North Korean army. The lesson to be learned from this is that, if the ROK army is forced to disperse its divisions because of the simultaneous invasion of North Korea and attack of guerrillas in home front areas, the Republic of Korea can be in a serious military danger, even though it maintains 80% of military divisions of North Korea. If the ROK army promotes the plans in [Military Reform Plan 2020], the number of military units of the ROK army will be 32.5% of that of the North Korean army. This ratio is 2.4 times lower than that of the time when the Korean war began, and in this case, 90% of total military power should be placed in the DMZ area. If 90% of military power is placed in the DMZ area, few troops will be left for the defense of home front. In addition, if the ROK army continues to reduce the troops, it can allow North Korea to have asymmetrical superiority in military force and it will eventually exert negative influence on the stability and peace of the Korean peninsular. On the other hand, it should be reminded that, during the Korean War, the Republic of Korea was attacked by North Korea, though it kept 53.3% of troops, compared to North Korea. It should also be reminded that, as of 2008, the ROK army is defending its territory with the troops 55.04% of North Korea. Moreover, the national defense is assisted by 25,120 troops of the US Forces in Korea. In case the total number of the ROK troops falls below 43.4% of the North Korean army, it may cause social unrest about the national security and may lead North Korea's misjudgement. Besides, according to Lanchester strategy, the party with weaker military power (60% compared to the party with stronger military power) has the 4.1% of winning possibility. Therefore, if we consider the fact that the total number of the ROK army troops is 55.04% of that of the North Korean army, the winning possibility of the ROK army is not higher than 4.1%. If the total number of ROK troops is reduced to 43.4% of that of North Korea, the winning possibility will be lower and the military operations will be in critically difficult situation. [Military Reform Plan 2020] rums at the reduction of troops and units of the ground forces under the policy of 'select few'. However, the problem is that the financial support to achieve this goal is not secured. Therefore, the promotion of [Military Reform Plan 2020] may cause the weakening of military defence power in 2020. Some advanced countries such as Japan, UK, Germany, and France have promoted the policy of 'select few'. However, what is to be noted is that the national security situation of those countries is much different from that of Korea. With the collapse of the Soviet Unions and European communist countries, the military threat of those European advanced countries has almost disappeared. In addition, the threats those advanced countries are facing are not wars in national level, but terrorism in international level. To cope with the threats like terrorism, large scaled army trops would not be necessary. So those advanced European countries can promote the policy of 'select few'. In line with this, those European countries put their focuses on the development of military sections that deal with non-military operations and protection from unspecified enemies. That is, those countries are promoting the policy of 'select few', because they found that the policy is suitable for their national security environment. Moreover, since they are pursuing common interest under the European Union(EU) and they can form an allied force under NATO, it is natural that they are pursing the 'select few' policy. At present, NATO maintains the larger number of troops(2,446,000) than Russia(l,027,000) to prepare for the potential threat of Russia. The situation of japan is also much different from that of Korea. As a country composed of islands, its prime military focus is put on the maritime defense. Accordingly, the development of ground force is given secondary focus. The japanese government promotes the policy to develop technology-concentrated small size navy and air-forces, instead of maintaining large-scaled ground force. In addition, because of the 'Peace Constitution' that was enacted just after the end of World War II, japan cannot maintain troops more than 240,000. With the limited number of troops (240,000), japan has no choice but to promote the policy of 'select few'. However, the situation of Korea is much different from the situations of those countries. The Republic of Korea is facing the threat of the North Korean Army that aims at keeping a large-scale military force. In addition, the countries surrounding Korea are also super powers containing strong military forces. Therefore, to cope with the actual threat of present and unspecified threat of future, the importance of maintaining a carefully calculated large-scale military force cannot be denied. Furthermore, when considering the fact that Korea is in a peninsular, the Republic of Korea must take it into consideration the tradition of continental countries' to maintain large-scale military powers. Since the Korean War, the ROK army has developed the technology-force combined military system, maintaining proper number of troops and units and pursuing 'select few' policy at the same time. This has been promoted with the consideration of military situation in the Koran peninsular and the cooperation of ROK-US combined forces. This kind of unique military system that cannot be found in other countries can be said to be an insightful one for the preparation for the actual threat of North Korea and the conflicts between continental countries and maritime countries. In addition, this kind of technology-force combined military system has enabled us to keep peace in Korea. Therefore, it would be desirable to maintain this technology-force combined military system until the reunification of the Korean peninsular. Furthermore, it is to be pointed out that blindly following the 'select few' policy of advanced countries is not a good option, because it is ignoring the military strategic situation of the Korean peninsular. If the Republic of Korea pursues the reduction of troops and units radically without consideration of the threat of North Korea and surrounding countries, it could be a significant strategic mistake. In addition, the ROK army should keep an eye on the fact the European advanced countries and Japan that are not facing direct military threats are spending more defense expenditures than Korea. If the ROK army reduces military power without proper alternatives, it would exert a negative effect on the stable economic development of Korea and peaceful reunification of the Korean peninsular. Therefore, the desirable option would be to focus on the development of quality of forces, maintaining proper size and number of troops and units under the technology-force combined military system. The tableau above shows that the advanced countries like the UK, Germany, Italy, and Austria spend more defense expenditure per person than the Republic of Korea, although they do not face actual military threats, and that they keep achieving better economic progress than the countries that spend less defense expenditure. Therefore, it would be necessary to adopt the merits of the defense systems of those advanced countries. As we have examined, it would be desirable to maintain the current size and number of troops and units, to promote 'select few' policy with increased defense expenditure, and to strengthen the technology-force combined military system. On the basis of firm national security, the Republic of Korea can develop efficient policies for reunification and prosperity, and jump into the status of advanced countries. Therefore, the plans to reduce troops and units in [Military Reform Plan 2020] should be reexamined. If it is difficult for the ROK army to maintain its size of 655,000 troops because of low birth rate, the plans to establish the prompt mobilization force or to adopt drafting system should be considered for the maintenance of proper number of troops and units. From now on, the Republic of Korean government should develop plans to keep peace as well as to prepare unexpected changes in the Korean peninsular. For the achievement of these missions, some options can be considered. The first one is to maintain the same size of military troops and units as North Korea. The second one is to maintain the same level of military power as North Korea in terms of military force index. The third one is to maintain the same level of military power as North Korea, with the combination of the prompt mobilization force and the troops in active service under the system of technology-force combined military system. At present, it would be not possible for the ROK army to maintain such a large-size military force as North Korea (1,190,000 troops and 86 units). So it would be rational to maintain almost the same level of military force as North Korea with the combination of the troops on the active list and the prompt mobilization forces. In other words, with the combination of the troops in active service (60%) and the prompt mobilization force (40%), the ROK army should develop the strategies to harmonize technology and forces. The Korean government should also be prepared for the strategic flexibility of USFK, the possibility of American policy change about the location of foreign army, radical unexpected changes in North Korea, the emergence of potential threat, surrounding countries' demand for Korean force for the maintenance of regional stability, and demand for international cooperation against terrorism. For this, it is necessary to develop new approaches toward the proper number and size of troops and units. For instance, to prepare for radical unexpected political or military changes in North Korea, the Republic of Korea should have plans to protect a large number of refugees, to control arms and people, to maintain social security, and to keep orders in North Korea. From the experiences of other countries, it is estimated that 115,000 to 230,000 troops, plus ten thousands of police are required to stabilize the North Korean society, in the case radical unexpected military or political change happens in North Korea. In addition, if the Republic of Korea should perform the release of hostages, control of mass destruction weapons, and suppress the internal wars in North Korea, it should send 460,000 troops to North Korea. Moreover, if the Republic of Korea wants to stop the attack of North Korea and flow of refugees in DMZ area, at least 600,000 troops would be required. In sum, even if the ROK army maintains 600,000 troops, it may need additional 460,000 troops to prepare for unexpected radical changes in North Korea. For this, it is necessary to establish the prompt mobilization force whose size and number are almost the same as the troops in active service. In case the ROK army keeps 650,000 troops, the proper number of the prompt mobilization force would be 460,000 to 500,000.

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