• The Plant Pathology Journal
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• 제21권4호
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• pp.343-348
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• 2005

The partial nucleotide sequences of the genomic dsRNA mycoviruses infecting Pleurotus ostreatus (isolates ASI2596, ASI2597, and Bupyungbokhoe) and Agaricus blazei Murrill were determined and compared with those of the other dsRNA mycoviruses. Partial nucleotide sequences of the purified dsRNA from ASI2596 and ASI2597 revealed RNA-dependent RNA polymerase sequences that are closely related to Oyster mushroom isometric virus 2, while nucleotide sequences and the deduced amino acid sequence from dsRNA mycovirus infecting Agaricus blazei did not show any significant homology to the other dsRNA mycoviruses. Specific primers were designed for RT-PCR detection of these dsRNA viruses and were found to specifically detect each dsRNA virus. Northern blot analysis confirmed the homogeneity of RT-PCR products to each purified dsRNA. Altogether, our results suggest that these virus-specific primer sets can be employed for the specific detection of each dsRNA mycovirus in infected mushrooms.

• 동력기계공학회지
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• 제18권6호
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• pp.140-145
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• 2014

Exploitation of renewable energies is on the increase to mitigate the reliance on fossil fuels and other natural gases with rocketing prices currently due to the depletion of their reserves not to mention their diverse consequences on the environment. Divergently, there are lots of industries "throwing" heat at higher temperatures as by products into the environment. This waste heat can be recovered through organic Rankine systems and converted to electrical energy with a waste heat recovery organic Rankine cycle system (WHR-ORC). This study uses the annual average condenser effluent from Namhae power plant as heat source and surface seawater as cooling source to analyze a waste heat recovery organic Rankine cycle using the Aspen HYSYS simulation software package. Hydrocarbon mixtures are employed as working fluid and varied in a ratio of 9:1. Results indicate that Pentane/Isobutane (90/10) mixture is the favorable working fluid for optimizing the waste heat recovery organic Rankine cycle at the set simulation conditions.

• Computers and Concrete
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• 제23권3호
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• pp.189-201
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• 2019

Recent advances in the concrete technology are aiding in minimizing the use of conventional materials by substituting by-products of various industries and energy sources. A large amount of stone waste i.e., dust and slurry form both are being originated during natural stone processing and causing deadily effects on the environment. The disposal problem of stone waste can be resolved effectively by using waste in construction industries. In present work, Kota stone slurry powder, as a substitution of cement was used along with accelerators namely calcium nitrate and triethanolamine as additives, to study their impact on various properties of the concrete mixtures. Kota stone slurry powder (7.5%), calcium nitrate (1%) and triethanolamine (0.05%) were used separately as well in combination in different concrete mixtures. Mechanical Strength, modulus of elasticity and electrical resistivity of concrete specimens of different mix proportions under water curing were studied experimentally. The durability properties in terms of strength and electrical resistivity against sulphate and chloride solution attack at various curing ages were also studied experimentally. Results showed that accelerators and Kota stone slurry powder separately enhanced the mechanical strength and electrical resistivity; but, their combination decreased strength at all curing ages. The durability of concrete specimens was also affected under the exposure to chemical attack too. Kota stone slurry powder found to be the most effective material among all materials. Material characterization was also done to study the microstructural properties.

• 대한구강악안면병리학회지
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• 제42권6호
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• pp.167-174
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• 2018

Natural products are vastly utilized as a source of chemotherapeutic agents for human cancers. Kochia scopraia is traditionally used for the cure of urological and dermatological diseases. Recently, methanol extract of Kochia scoparia (MEKS) has been shown to have anti-cancer activity to various human cancers. However, there is no report demonstrating the anti-cancer activity of MEKS in human mucoepidermoid carcinoma (MEC) cells. In this study, the authors studied the effects of MEKS on the cell proliferation and underlying mechanism in YD15 human MEC cells. MEKS decreased YD15 cell proliferation proven by trypan blue exclusion assay and induced apoptosis, evidenced by cell cycle analysis and western blotting. Autophagy induction by MEKS was verified by western blotting. In addition, MEKS regulated the expression of phosphorylated Akt, phosphorylated p38 and Nrf2 protein. This results can imply that MEKS might be a potential candidate for the treatment of human MEC cells.

• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1288-1294
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• 2021

There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-O-galloyl-glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.

• Journal of Forest and Environmental Science
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• 제36권4호
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• pp.318-325
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• 2020

This study was carried out to find the effects of aromatherapy using natural fragrance on reducing stress and improving job satisfaction of transportation workers in the taxi industry. A total of 120 workers were divided into the experimental group and the control group according to the inhalation of the fragrance and tested for 2 weeks. After 2 weeks, the difference of emotional change, stress analysis, and evaluation of job satisfaction according to aromatherapy were compared between the two groups. The result showed that the experimental group had a higher interest in scent products than the control group and that they wanted to encounter a lot of real scents, indicating that aromatherapy caused positive interest in general scent likability. In addition, the experimental group said that the inhalation of fragrance would refresh the feeling of the passengers in the vehicle and smoothly communicate with the passengers. Especially, it showed a significant difference between the two groups in terms of tension relief, pleasure and good mood by inhalation of fragrance, indicating the positive effect on job satisfaction.

• Journal of Microbiology and Biotechnology
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• 제32권3호
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• pp.302-306
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• 2022

A chemical investigation of a culture extract from Streptomyces sp. RK85-270 led to the isolation and characterization of two new oxindoles, RK-270D (1) and E (2). The structures of 1 and 2 were determined by analyzing spectroscopic and spectrometric data from 1D and 2D NMR and High-resolution electrospray ionization mass spectrometry (HRESIMS) experiments. Compound 1 exhibited anti-angiogenic activities against human umbilical vein endothelial cells (HUVECs) without cytotoxicity. Results of Western blot analysis revealed that 1 inhibits VEGF-induced angiogenesis in the HUVECs via VEGFR2/ p38 MAPK-mediated pathway.

• Natural Product Sciences
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• 제27권4호
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• pp.284-292
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• 2021

Recently, a phytoestrogenic functional food has been developed using the fruits of Sophora japonica. Phytochemical investigation of fruits of S. japonica led to the isolation of eight flavonoid glycosides using various chromatographic techniques. The isolated compounds were identified as genistin (1), sophoricoside (2), genistein 7,4'-di-O-β-D-glucopyransoide (3), sophorabioside (4), genistein-7-O-β-D-glucopyranoside-4'-O-[(α-L-rhamnopyranosyl)-(1→2)-β-D-glucopyranoside] (5), sophoraflavonoloside (6), nicotiflorin (7) and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside (8), respectively, by comparison of their spectroscopic data with those reported in the literature. In addition, a new HPLC-DAD method for simultaneous determination of the isolated compounds was developed to quantitate the contents of flavonoids in S. japonica and S. flavescens. The method was validated in terms of limit of detection, limit of quantitation, specificity, linearity, precision and accuracy. The validated method was successfully applied to determine eight flavonoids in two Sophora species. The contents of eight flavonoids varied according to the parts and species. Particularly, it was found that only the fruits of S. japonica contained sophoricoside, a phytoestrogenic isoflavone.

• 대한약침학회지
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• 제25권3호
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• pp.186-198
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• 2022

Objectives: This overview summarizes the evidence for the effectiveness and safety of complementary therapies for insomnia through systematic reviews (SRs) and meta-analyses (MAs). Methods: A comprehensive literature search on SRs and MAs for complementary therapies for insomnia was conducted using six databases. Results: This overview included 30 SRs/MAs. The Pittsburgh Sleep Quality Index (PSQI) analysis of insomnia included 383 randomized controlled trials and 31,748 participants. Twenty-four SRs/MAs (80%) out of 30 SRs/MAs reported a positive result, and six SRs/MAs reported a partially positive result. The most frequently used form of acupuncture reported a positive effect in 89.5% (17/19) of SRs/MAs and a superior effect to western medicine, sham/placebo, and no treatment. The lack of protocol registration and the excluded studies list resulted in generally poor methodological and reporting quality in SRs/MAs with AMSTAR 2. Conclusion: This overview confirmed that complementary therapies positively affected the PSQI scores in patients with insomnia. However, better-designed primary studies are needed to strengthen the relevant evidence in the future. A more stringent assessment of multiple systematic reviews 2 must be followed when performing SR and MA.

• 생명과학회지
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• 제20권1호
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• pp.60-65
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• 2010

파속류에 다량 함유된 황화합물들은 GC/ECD에 민감하게 반응하기에, 이 농산물들의 잔류농약분석에서 방해 물질로 작용하여 농약성분의 분리정량에 어려움을 주고 있다. 최근 잔류농약분석에서 정제법으로 여러 가지 고상추출법(solid-phase extraction)이 개발되고 있으며, 본 연구에서는 황화합물을 효율적으로 제거하기 위하여, 20% 질산은($AgNO_3$)용액 3 ml을 Florosil cartridge (1 g packing, 6 ml tube)에 처리한 은이온흡착 cartridge (silver nitrate impregnated florosil cartridge)를 조제하였다. 이 cartridge를 이용하여 파속류 각 2, 4, 6 및 10 g에 해당하는 추출액을 고상추출법으로 정제한 결과, 양파 6 g, 대파 4 g, 쪽파는 4 g 까지는 황화합물의 95% 이상을 제거할 수 있었다. 그리고, 40종의 농약을 각 파속류에 첨가하고 이 cartridge에서 유출하여 회수율을 분석한 결과 34종이 70~120%의 범위 안에 들었다.