• Title/Summary/Keyword: Natural products analysis

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Identification study of rare and high-priced natural products used for oriental medicine by RAPD analysis (RAPD 분석법을 이용한 산삼, 웅담, 녹용 등의 한약재 판별연구)

  • Cho, Dong-Wuk
    • Korean Journal of Oriental Medicine
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    • v.1 no.1
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    • pp.471-476
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    • 1995
  • Natural products used for oriental medicine often come from various geographical sources, after several different distribution channels. Therefore some form of quality control procedure is required to safeguard naturl products for prescriptions purposes. To achieve this, systematic apprroaches such as morphological examination, microscopic analysis of powdered herbs and chemical analysis can be carried out. However, to ensure absolute criteria for quality assurance of natural products, DNA fingerprinting method such as RAPD(Random amplified polymorphism DNA) analysis can be used for authentication of natural products for authenticatin of natural products. In this study, warious oligonucleotide primers will be synthesized for the detection of RAPD markers and also parameters of affecting PCR(Polymerase Chain Reaction) in the detection of RAPD markers of rare and high-priced natural products will be studied with genomic DNA of chosen samples.

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Analysis of the Textiles Design of Natural Indigo Dyed Products in Korea and Japan -Focusing on the Natural Indigo Dyed Products of Internet Shopping Malls- (한국과 일본의 쪽 염색 제품의 텍스타일 디자인 비교 -인터넷 쇼핑몰의 쪽 염색 제품을 중심으로-)

  • Lee, Mi-Suk;Chung, Kyung-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.3
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    • pp.359-370
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    • 2011
  • This study analyzes the textiles design of natural indigo dyed products in Korea and Japan. In this study, a total of 556 Korean natural indigo dyed products, and 2,730 Japanese natural indigo dyed products were used for analysis. The subjects of this study were 556 natural indigo dyed products and 2,730 Japanese natural indigo dyed products selling natural indigo dyed products which were found using search engine keywords of natural indigo dyeing and natural dyeing. Research and analysis was treated regarding the products, items, patterns, and the representation techniques of the patterns. The results of this study are as follows. In the pattern used for natural indigo dyed products, 71.4% of Korean products have no pattern, but 77.1% of Japanese products have patterns. On the representation techniques of the patterns, Korean products used tie-dyeing and a dip patterned fabric. While in the Japanese products, the most frequent patterning techniques were paraffin dye, followed by tie-dyeing, yarn-dyed and weaving, screen printing, and yarn-dyed and knitting. Regarding the kinds of patterns for natural indigo dyed products, only 8 kinds of patterns were used in Korean products; however, over 50 kinds of various patterns were used in Japanese products. Most patterns in the Korean products were ion patterns made by tie-dyeing. While in the Japanese products, the most frequent patterns were stripe patterns, followed by flower, dot, and ion patterns. Based on these research results, the problems of the textile design of Korean natural indigo dyed products were that most of the products have no pattern, and even though there were patterns, they lacked variations between the products. While in the case of Japan, they used the traditional and modem patterns of various textile representation techniques.

Salternamide E from a Saltern-derived Marine Actinomycete Streptomyces sp.

  • Kim, Seong-Hwan;Shin, Yoonho;Lee, Sang Kook;Shin, Jongheon;Oh, Dong-Chan
    • Natural Product Sciences
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    • v.21 no.4
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    • pp.273-277
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    • 2015
  • Comprehensive chemical analysis of extracts and fractions of marine actinomycete strains led to the discovery of a new minor secondary metabolite, salternamide E (1), from a saltern-derived halophilic Streptomyces strain. The planar structure of salternamide E (1) was elucidated by a combinational analysis of spectroscopic data including NMR, MS, UV, and IR. The absolute configuration of salternamide E (1) was determined by circular dichroism spectroscopic analysis. Salternamide E displayed weak cytotoxicity against various human carcinoma cell lines.

Molecular Identification and Chemical Analysis of Aconiti Kusnezoffii Tuber on the Domestic Markets (국내 시장에서 유통되는 초오의 DNA 감별과 화학적 분석)

  • Jang, Hyeri;Joe, Kyeong-Hwa;Song, Kwangho;Lee, Kyoung Jin;Park, Sait Byul;Lee, Chaemin;Ha, In Jin;Lee, Kyungjin;Suh, Youngbae;Kim, Yeong Shik
    • Korean Journal of Pharmacognosy
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    • v.49 no.2
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    • pp.145-154
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    • 2018
  • Aconiti Kusnezoffii Tuber has been traditionally used to treat the symptoms of rheumatoid arthritis and joint pain. The main constituents are diterpenoid alkaloids such as benzoylmesaconine, benzoylaconine, mesaconitine, aconitine, and hypaconitine. In Korea, Aconiti Kusnezoffii Tuber is officially defined as the tubers of Aconitum kusnezoffii Reichb., A. ciliare Decasisne, and A. triphyllum Nakai. On the other hand, only the tuber of A. kusnezoffii is to be used in China. In order to identify the botanical origin of Aconiti Kusnezoffii Tuber circulated in Korea, we analyzed 24 samples of Aconiti Kusnezoffii Tuber obtained from local markets for comparative DNA analysis. The sequence analysis of nrRNA ITS 1 was useful to distinguish Aconitum species and revealed that the roots of A. karakolicum were circulated in Korean markets without discretion. HPLC quantitative analysis showed that aconitine was detected at the highest amount in A. karakolicum. Authentic diterpenoid alkaloids were coinjected for quantification of aconitine-type ingredients. All data were statistically grouped by Principal Component Analysis (PCA). This study suggests that both molecular and chemical analyses should be utilized for the standardization and the quality control for Aconiti Kusnezoffii Tuber.

An analysis of natural products patents (천연물 신약 특허 동향 분석)

  • Han, Yoo-Jin;Park, Sunju
    • Journal of Society of Preventive Korean Medicine
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    • v.20 no.2
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    • pp.77-86
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    • 2016
  • Objectives : This study aims to investigate the current situation of domestic patents in Korea on natural products and to provide directions for developing and applying herbal medicinal products by in-depth analyses. Methods : Combinations of words, such as "A61K" or "A61P", "herb" or "Korean medicinal herb" or "Korean medicine" or "traditional" or "natural" or "$botanic^*$" or "China", and "medicine" or "treatment" or "prevention" or "improvement", were used to search patents in the World Intellectual Property Service (WIPS) database. Three methods were applied to analyze natural products patents. First, the number of patent registrations was sorted by year. Second, the assignees were analyzed by count and country of origin. Finally, cluster map analysis was conducted to explore frequently emerging words in natural products and the relationship amongst those words to treat corresponding diseases/technologies. Results : Results showed that, first, the total number of patents had been increasing. Among the patents, 76.4% were registered by domestic institutes/companies, and 23.6% by foreign institutes/companies. Second, USA, Japan, and China possess a considerable number of Korean patents and, therefore, domestic institutes/companies can seek joint technological development opportunities with their counterparts from those countries in the future. Finally, a total of four clusters were identified by cluster map analysis. Each of the clusters includes natural products related to diseases involving skin, aging, and blood sugar, as well as adult diseases. Conclusions : In this study, natural products patents registered in the Korean Intellectual Property Office were analyzed. The analyses results showed the kinds of natural products that had been employed for the treatment of certain diseases. However, natural products included in existing patents were minimal given that 4,174 species of indigenous plants are found in Korea. Thus, Korean institutes/companies should utilize unexplored plants to develop more value-added drugs.

Identification and classification study of natural products by RAPD analysis (RAPD(Random Amplified Polymorphic DNA)법을 이용한 한약재의 판별 연구)

  • Kim, Dae-Weon;Kim, Do-Kyun;An, Sun-Kyong;Cho, Dong-Wuk
    • Korean Journal of Oriental Medicine
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    • v.3 no.1
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    • pp.153-167
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    • 1997
  • Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition, sing these methods, however, is not satisfying for the precise identification of natural products because they are often valiable in the compositions and morphology To standardize the natural products identification and classification, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notoginseng, were genetically analyzed by RAPD. Also, DNA isolated from Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctos, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification and classification of other natural products.

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A study on the Purchase Appraisal Standard and Post-purchase Satisfaction of Natural Dyeing Products (천연염색제품 구매 평가기준 및 구매 후 만족도)

  • Park, Young-Hee
    • Fashion & Textile Research Journal
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    • v.14 no.1
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    • pp.64-74
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    • 2012
  • The purpose of this study was to find purchase appraisal standard and post-purchase satisfaction for natural dyeing products. The data used for this study were collected from the women in their from 20's to 50's who are living in the regions of Busan, Ulsan, and Gyeongnam in Korea. The questionnaire of 537 copies was used for a statistical analysis. To analyze data, 2-test, t-test, Cronbach's a test, factor analysis, ANOVA, Duncan test, regression analysis, etc. were carried out. A used statistical package was SPSS 14.0. The analysis results were as follows. The purchase appraisal standards for natural dyeing products were identified as practicality and reliability, suitability of use, symbolic property of status, design property, and convenience of buying. As the analysis result for purchase appraisal standard according to the demographic characteristics, the purchase appraisal standard according to most demographic characteristics showed significant difference. The factors of post-purchase satisfaction for natural dyeing products were verified as product modification and durability, design and price, hygienic property and product quality. As the analysis results of post-purchase satisfaction for natural dyeing products according to demographic characteristics, the post-purchase satisfaction according to demographic characteristics showed significant difference. The factors affecting post-purchase satisfaction were verified as product quality, kinds of item, color change, product lifespan, color change, etc. Consequently, because the purchase appraisal standard of natural dyeing product is very various, it is necessary to enhance post-purchase satisfaction of natural dyeing products by reflecting these factors in advance at the product development stage.

Study of Simultaneous Analysis of Indicator Components of DTP(Digital Textile Printing) Textile Products Using HPLC-MS/MS - Focusing on Natural Dyes - (HPLC-MS/MS를 활용한 DTP(Digital Textile Printing) 제품의 지표성분 동시분석법 연구 - 천연염료를 중심으로 -)

  • Lee, Wonkyoung;Sung, Eunji;Moon, Joung Ryul;Kim, Miji;Kim, Jonghoon
    • Fashion & Textile Research Journal
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    • v.23 no.6
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    • pp.844-851
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    • 2021
  • Due to the increase in consumers' interest about well-being, interest in eco-friendly products has been increasing due to the harmful effects of various harmful substances contained in textile products and environmental issues. As a result, natural dyes of less potential risk than synthetic dyes and digital textile printing(DTP) textile products with less environmental pollution are drawing attention. However, due to the lack of evaluation criteria for DTP textile products with natural ink and the nature of many colors are stacked layer by layer for dying, the need for simultaneous analysis is emerging. To evaluate whether the natural dye is derived from natural ingredients, the biocarbon content is analyzed. However, in the case of ink made using natural dyes and DTP textile products using natural ink, it is difficult to analyze the biocarbon content due to the limitation of the presence of a small amount of dye contained therein. In this study, we were shown the possibility of natural derived verification by cross-checking the analytes of natural dyes (Persicaria tinctoria, an indigo dye; Dactylopius coccus, a light red; and Curcum longa L., i.e., turmeric) and natural ink with HPLC-MS/MS. The coefficient of determination was 0.999 or higher, the limit of quantification was 0.647-3.664 ㎍/L and a %RSD of each indicator material was less than 10. Then, the extraction amount of natural dyes for five patterned fabrics was analyzed.

A Study on the Impact of the Pursuit Benefits in Clothing Materials in the Intent to Purchase Natural Dyeing Products: Focusing on the Mediating Effect of the Attitude toward Natural Dyeing Products (의류소재 추구혜택이 천연염색제품의 구매의도에 미치는 영향에 관한 연구: 천연염색제품에 대한 태도의 매개효과를 중심으로)

  • Shin, Kyung Ha
    • Journal of the Korea Fashion and Costume Design Association
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    • v.24 no.3
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    • pp.97-109
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    • 2022
  • The purpose of this study is to examine the structural relationship among the pursuit benefits in clothing materials, the attitude toward natural dyeing products, and the purchase intent among consumers interested in natural dyeing products. For this study, 489 men and women in their 20s to 60s were surveyed, and the data was analyzed using SPSS 23.0 and AMOS 23.0 programs. The findings of the analysis were as follows. First, of the pursuit benefits in clothing materials, function and originality factors had a significant and positive impact on the purchase intent of natural dyeing products. Second, of the pursuit benefit of clothing materials, functions, sustainability, and originality significantly and positively impacted the emotional and cognitive attitude toward natural dyeing products. Third, the emotional and cognitive attitude toward natural dyeing products had a significantly positively impacted the purchase intent. Fourth, in the relationship between the pursuit benefits in clothing materials and the purchase intent of natural dyeing products, the emotional and cognitive attitudes toward natural dyeing products were shown to have partial mediating effects.