• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.5
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• pp.1170-1175
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• 2007

We have examined the effect of water extract of Phellinus linteus, a raw material of Korean traditional herbal medicine, on the induction of HL-60 cell differentiation. The proliferation of HL-60 cell was inhibited dose-dependently by treatment with various doses of P. linteus extract. It also caused a significant change in NBT reduction (7.5 times). The expression of CD11b and CD14 was increased in the cells treated with the extract, especially in those arrested at G0/G1 stage, which suggested that some components in P. Linteus extract induced HL-60 cell differentiation to granulocytic and monocyte lineages. Moreover, the expression levels of $p21^{WAF1/CIP}$ and $p27^{KIP}$ were up-regulated during HL-60 cell differentiation induced by P. Linteus extract. These results together suggest that P. Linteus extract contains potential HL-60 cell differentiation agents.

• The Korean Journal of Food And Nutrition
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• v.31 no.5
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• pp.631-639
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• 2018

The purpose of this study was to investigate the characteristics of macaroons prepared using natural color materials (Opuntia ficus-indica var. saboten (Re-N), mulberry leaf (Gr-N), pumpkin (Ye-N), and cocoa powder (Br-N)) and corresponding artificial food colorants (red (Re-A), yellow (Ye-A), green (Gr-A) and brown (Br-A)). The moisture content of macaroons prepared using natural color material was higher compared to the macaroons prepared using artificial food colorant. DPPH and ABTS radical scavenging activity was similar in both types of macaroons. Lightness and redness of Re-N, Gr-N, Ye-N, and Br-N macaroons were lower than Re-A, Gr-A, Ye-A and Br-A macaroons. Yellowness of Re-N was higher because of the browning reaction. The texture profile analysis revealed lower hardness when natural color powder was employed. In the sensory evaluation, overall acceptability was not significantly different between natural colored and artificial colored macaroons. As a result, macaroons with the natural color material were softer and showed higher antioxidative activity, however, organoleptic properties were not much different when compared with macaroons with artificial colors. Apparently, it is stated that more studies on the development of macaroons with more enhanced physical functionality and good taste using natural materials should be performed.

• New & Renewable Energy
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• v.8 no.2
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• pp.24-32
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• 2012

Natural gas hydrates have a high potential as the 21st century new energy resource, because it have a large amount of deposits in many deep-water and permafrost regions of the world widely. Natural gas hydrate is formed by physical binding between water molecule and gas mainly composed of methane, which is captured in the cavities of water molecules under the specific temperature and pressure. $1m^3$ methane hydrate can be decomposed to the methane gas of $172m^3$ and water of $0.8m^3$ at standard condition. Therefore, there are a lot of practical applications such as separation processes, natural gas storage transportation and carbon dioxide sequestration. For the industrial utilization of methane hydrate, it is very important to rapidly manufacture hydrate. However, when methane hydrate is artificially formed, its reaction time may be too long and the gas consumption in water becomes relatively low, because the reaction rate between water and gas is low. So in this study, hydrate formation was experimented by adding natural zeolite and Synthetic zeolite 5A in distilled water, respectively. The results show that when the Synthetic zeolite 5A of 0.01 wt% was, the amount of gas consumed during the formation of methane hydrate was higher than that in the natural zeolite. Also, the natural zeolite and Synthetic zeolite 5A decreased the hydrate formation time to a greater extent than the distilled water at the same subcooling temperature.

• Journal of Microbiology and Biotechnology
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• v.22 no.1
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• pp.50-57
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• 2012

Phospholipase C-${\gamma}l$ (PLC-${\gamma}l$) expression is associated with cellular transformation. Notably, PLC-${\gamma}$ is up-regulated in colorectal cancer tissue and breast carcinoma. Because exotoxins released by Clostridium botulinum have been shown to induce apoptosis and promote growth arrest in various cancer cell lines, we examined here the potential of Clostridium difficile toxin A to selectively induce apoptosis in cells transformed by PLC-${\gamma}l$ overexpression. We found that PLC-${\gamma}l$-transformed cells, but not vector-transformed (control) cells, were highly sensitive to C. difficile toxin A-induced apoptosis and mitotic inhibition. Moreover, expression of the proapoptotic Bcl2 family member, Bim, and activation of caspase-3 were significantly up-regulated by toxin A in PLC-${\gamma}l$-transformed cells. Toxin A-induced cell rounding and paxillin dephosphorylation were also significantly higher in PLC-${\gamma}l$-transformed cells than in control cells. These findings suggest that C. difficile toxin A may have potential as an anticancer agent against colorectal cancers and breast carcinomas in which PLC-${\gamma}l$ is highly up-regulated.

• Mycobiology
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• v.29 no.3
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.11a
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• pp.42-42
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• 2011

To evaluate the Hwangsu spring as natural mordants effect on dyeing. The natural mordants effect of Hwangsu spring were analyzed by pH, temperature, trace elements, organic matter and color fastness. Hwangsu spring having pH 2.42 and $14.4^{\circ}C$ was harvested at the end of September, in Yeongcheon (Gyeongbuk Province, Korea) and left on a $0^{\circ}C$ and $25^{\circ}C$ before use. Hwangsu spring during storage at $25^{\circ}C$ turned brown by photochemical reaction. The Result of ICP analysis, it contained Fe(414.9 ppm), Al(88.9 ppm), Mn(4.9 ppm) and observed character by water analysis. Dyeing and post-mordant procedure; Cotton(KS K 0905) were dyed with clove powder for 30 min at $70^{\circ}C$. Hwangsu spring, Aluminium Sulfate $14-18H_20(Al_2(SO_4)_3{\cdot}14-18H_2O)$, Iron Sulfate Heptahydrate($FeSO_4{\cdot}7H_2O$) were used by post mordant at the same ratio. The dyed fabrics were treated with 20% each mordant solution at $25^{\circ}C$ for 10min. Comparison with a reproduced chemical mordant, the K/S values of cotton fabrics dyed with Hwangsu sping were increased.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.122-122
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• 2012

The aims of this study were to examine the efficacy of phytochemical compounds of colorants as anti-oxidant agent. The bioactive properties of natural colorants were studied by total phenolic contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and anti-bacterial activity in Escherichial coli. The concentration ($IC_{50}$) of various extracts from colorants required to exert 50% reducing effect on DPPH radical were found to be 0.007-4 mg/ml. Especially, the gallnut (Schlectendalia Chinensis) displayed remarkable effect of DPPH as compared to positive control ascorbic acid. The total phenolic contents (TPC) and restraint of E coli. also analyzed. It was found that gallnut extracts effectively inhibited DPPH radical at a concentration below 0.01 mg/ml. Natural colorant extracts could be of good resources as anti-oxidant and anti-bacterial agents. The results suggest that our study may contribute to the development of natural and functional materials with potential application to reduce oxidative damage.

• Journal of the Korea Institute of Building Construction
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• v.4 no.2
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• pp.105-112
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• 2004

The objective of this study is to observe today's situation and direction in relation with the natural air-conditioning and heating system to expand the architectural utilization of natural management for human comfort and energy conservation in architectural design. Also this study to provide the fundamental knowledge and. design-techniques on environment-friendly architecture in connection with 'natural idea in architecture'. In accordance with this intention, this thesis is composed of the following contents : 1) to understand the fundermental concepts about natural air-conditioning and heating system, 2) to review the traditional characteristics in Korean and Southeast Asian architecture related to natural air-conditioning and heating system, 3) to examine modern design-techniques in relation with natural air-conditioning and heating system in the selected area, 4) to attempt to synthesize the whole situation. Environment-friendly architecture using natural ideas and design-techniques in architecture is very important architectural aspect in a current architecture, also for the future. In spite of general considerations, it is expected that this study is use to understand the principle and practical application of natural air-conditioning and heating system in architectural design.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.5 s.118
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• pp.59-69
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• 2006

Nature-learning place is a critical space together with nature center in Natural parks. But there are serious faults on these design and planning as for the landscape ecological consideration. The objective of this paper was to suggest the ecological alternatives through analyzing existing cases of nature-teaming place in Natural parte. Five case sites in Natural parks were selected in Chollabukdo. Three landscape indices- subjectivity, natural prototype, nativity, were introduced for evaluating the existing cases. Results showed that those are short of landscape ecological senses- Biotope target species, ecological vegetation patches, eco-corridor concept. A proposal for the ecological planning in Deokyusan Natural park was suggested according to several criteria with biotopes as forest and stream patches and the corridor. The results of the study can be summarized as follows. First, existing natural-loaming sites should be evaluated in light of landscape ecological indices. Second, they can be designed to work with the concept of the biotope patch and corridor for target species. The results of this study could provide critical guidelines for the ecological planning and design of natural-loaming sites in nature parks.

• Nuclear Engineering and Technology
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• v.54 no.3
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• pp.826-833
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• 2022

A two-phase natural circulation test using SMART integral test loop (SMART-ITL) was conducted to explore thermo-hydraulic phenomena of two-phase natural circulation in the SMART reactor. Specifically, the test examined the natural circulation in the primary loop under a stepwise coolant inventory loss while keeping the core power constant at 5% of the scaled full power. Based on the test results, three flow regimes were observed: single-phase natural circulation (SPNC), two-phase natural circulation (TPNC), and boiler-condenser natural circulation (BCNC). The flow rate remained steady in the SPNC, slightly increased in the TPNC, and dropped abruptly and maintained in the BCNC. Using a natural circulation flow map, the natural circulation characteristic in the SMART-ITL was compared with those in pressurized water reactor simulators. In the SMART-ITL, a BCNC regime appeared instead of siphon condensation and reflux condensation regimes because of the use of once-through steam generators.