• Biomolecules & Therapeutics
• /
• 제31권5호
• /
• pp.484-495
• /
• 2023

Idiopathic pulmonary fibrosis (IPF) can be defined as a progressive chronic pulmonary disease showing scarring in the lung parenchyma, thereby resulting in increase in mortality and decrease in the quality of life. The pathophysiologic mechanism of fibrosis in IPF is still unclear. Repetitive microinjuries to alveolar epithelium with genetical predisposition and an abnormal restorative reaction accompanied by excessive deposition of collagens are involved in the pathogenesis. Although the two FDA-approved drugs, pirfenidone and nintedanib, are under use for retarding the decline in lung function of patients suffered from IPF, they are not able to improve the survival rate or quality of life. Therefore, a novel therapeutic agent acting on the major steps of the pathogenesis of disease and/or, at least, managing the clinical symptoms of IPF should be developed for the effective regulation of this incurable disease. In the present review, we tried to find a potential of managing the clinical symptoms of IPF by natural products derived from medicinal plants used for controlling the pulmonary inflammatory diseases in traditional Asian medicine. A multitude of natural products have been reported to exert an antifibrotic effect in vitro and in vivo through acting on the epithelial-mesenchymal transition pathway, transforming growth factor (TGF)- β-induced intracellular signaling, and the deposition of extracellular matrix. However, clinical antifibrotic efficacy of these natural products on IPF have not been elucidated yet. Thus, those effects should be proven by further examinations including the randomized clinical trials, in order to develop the ideal and optimal candidate for the therapeutics of IPF.

• 해양환경안전학회지
• /
• 제22권5호
• /
• pp.483-489
• /
• 2016

본 연구는 USCG phase-II의 형식승인 기준인 자연상태 생물군집의 75 % 이상 유지하여야 하는 평가체계에 대비하여 자연생물군집 농축 및 선박평형수관리시스템(Ballast Water Management System, BWMS) 처리 전 후 생물사멸시험을 실시하였다. 자연 식물플랑크톤군집의 농축 조사는 중영양수계인 장목만과 부영양화수계의 마산만에서 동계에 수행하였다. 장목만과 마산만에서 1톤 기준으로 생물을 농축하였을 경우, $10-50{\mu}m$ 크기 생물 현존량은 $4.7{\times}10^4cells\;mL^{-1}$과 $0.8{\times}10^4cells\;mL^{-1}$ 이었고, 농축생물의 생존율은 90.4 %와 88.0 %로 각각 나타났다. 특히 장목만에서는 Skeletonema costatum-like species 같은 체인을 형성하는 소형 규조류가 극우점한 반면, 마산만에서는 $<10{\mu}m$보다 작은 편모조류 및 체인을 형성하지 않는 대형 와편모조류(Akashiwo sanguinea, Heterocapsa triquetra)가 우점하였다. 이와 같은 우점종 세포크기의 차이로 장목만 농축효율이 마산만보다 높게 나타났다. BWMS 장비를 통과한 처리 당일 생물 사멸률은 장목만이 90.4 %로, 마산만의 93 %보다 약간 낮았고, 장목만에서 BWMS 처리 5일 경과 후, 대조군의 대상생물의 사멸률은 6.7 %로 나타났다. 처리군에서는 >99 %로 대부분 사멸되어, 시험생물로서의 적용 가능성을 확인할 수 있었다. 결과적으로 동계와 같이 해역내 생물량이 낮을 경우, 주간 8시간 수행한 네트의 생물농축만으로 는 USCG Phase II의 형식승인 기준인 500톤 탱크에 $1.0{\times}10^3cells\;mL^{-1}$ 이상으로 자연생물 개체수 밀도를 충족하기는 쉽지 않다는 것을 파악하였고, 이를 보완하기 위해서는 일정기간 자연생물을 대량 배양 및 채집할 수 있는 시스템 도입이 필요할 것으로 판단된다.

• 농약과학회지
• /
• 제10권2호
• /
• pp.107-116
• /
• 2006

한국산 곤충병원성 선충의 녹색콩풍뎅이(Popillia quadriguttata), 주황긴다리풍뎅이(Ectinohoplia rufipes) 및 연다색풍뎅이(Phyllopertha diversa)에 대한 병원성을 실내에서 검정하였으며 아울러 Heterorhabditis sp. KCTC 0991BP 계통을 이용하여 굼벵이 방제효과와 지속성을 조사하였다. 실내실험 결과 녹색콩풍뎅이 3령충에 대하여 H. bacteriophora Hamyang 계통은 95%의 높은 치사율을 보였으며 주황긴다리풍뎅이 3령충에 대해서도 H. bacteriophora Hamyang 계통과 Heterorhabditis sp. KCTC 0991BP 계통이 80% 이상의 치사율을 나타내었다. 그러나 연다색풍뎅이는 병원성이 가장 높은 Heterorhabditis sp. KCTC 0991BP 계통 처리 시에도 60%의 치사율을 보였다. 골프장에서 Heterorhabditis sp. KCTC 0991BP 계통을 $2.24{\times}10^9$ Ijs $ha^{-1}$ 농도로 처리하였을 때 굼벵이의 밀도는 39%까지 감소되었고, 골프장 페어웨이에서 지속성은 $5{\sim}10$ cm 깊이의 토양에서 두 달까지 지속되었으나 $0{\sim}5$ cm 깊이에서는 치사되는 미끼곤충(꿀벌부채명나방)이 없어 지속성이 낮았다.

• 아시안잔디학회지
• /
• 제19권1호
• /
• pp.17-25
• /
• 2005

무궁화의 중요한 식엽성 해충인 무궁화잎밤나방(Anomis mesogona)과 큰붉은잎밤나방(Anomis commoda)에 대한 곤충병원성 선충 Steinernena carpocapsue 포천 계통(ScP)의 병원성을 실내 petri dish와 pot에서 령기별(2령, 3-4령, 5령)로 검정하였다. 큰붉은잎밤나방과 무궁화잎밤나방에 대한 반수치사농도$(LC_{50})$는 령기가 높아질수록 증가하여 선충에 대한 감수성이 감소하였는데, 2령충의 경우 각각 9.7과 4.5였다. 그리고 Pot에서도 ScP의 병원성은 령기에 따라 차이를 보였다. ScP는 큰붉은잎밤나방과 무궁화잎밤나방의 2령충에 대하여 가장 높은 치사율을 보였는데, 90,000마리/pot($=1\times10^9$ 마리/ha) 처리 시 각각 $72.5\%$와 $87.5\%$의 치사율을 보였다. 반면 3-4령충부터는 병원성이 감소하여 큰붉은잎밤나방과 무궁화잎밤나방 5령충에 대해서는 처리 5일 후, 90,000마리/pot 처리 시 각각 $5.0\%$와 $10.0\%$의 치사율만 나타내었다. Pot에 2령충과 3-4령충, 5령충을 각각 10마리씩 접종한 후, ScP를 90,000마리/pot 농도로 처리하였을 때, 큰붉은잎밤나방과 무궁화잎밤나방의 치사율은 각각 $69.2\%$와 $50.0\%$였다.

• 한국수산과학회지
• /
• 제46권5호
• /
• pp.598-606
• /
• 2013

The blackfin flounder Glyptocephalus stelleri is a commercially important species in the East Sea of Korea, but its catches and biomass have decreased gradually in recent years. This study estimated the optimal catch (acceptable biological catch, ABC) for the effective management of this species by estimating population ecology parameters and the stock biomass of blackfin flounder in the East Sea of Korea. The estimated instantaneous coefficient of total mortality (Z) of blackfin flounder was 1.0542/year, the survival rate (S) was 0.3485, and the instantaneous coefficient of natural mortality (M) was 0.3637/year. From the values of S and M, the instantaneous coefficient of fishing mortality (F) was calculated to be 0.6905/year. The age at first capture was 1.304 years, and the total length was 11.5 cm at that time. On the basis of these parameters, the annual biomass was estimated by a biomass-based cohort analysis using annual catch data in weight by year for 1991-2012 in the East Sea of Korea. The annual biomass peaked in 1997 at about 12,800 mt and then subsequently declined continuously to a level of 10,500 mt in 2004 and to 9,800 mt in 2011 and 2012. The maximum sustainable yield and $F_{0.1}$ were estimated as 3,547 mt and 0.3595/year, respectively. Using these estimations, the ABC was estimated to be 3,571 mt in tier 5, 3,397 mt in tier 4, and 2,622 mt in tier 3.

• Mycobiology
• /
• 제43권2호
• /
• pp.137-149
• /
• 2015

In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration ($LC_{50}$) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권9호
• /
• pp.1253-1259
• /
• 2001

Three cassava varieties, namely MM 92 (MM), Black Twig (BT) and Local (LC), were arranged in a randomized complete block design to evaluate their dry fodder and crude protein (CP) productivity as well as growth persistency. Cassava plants grown in small plots of $5m{\times}10m$ at a planting distance of $25cm{\times}25cm$ were harvested every 6 weeks starting from 3 months after planting. Dry fodder yields of MM, BT and LC over the 8 harvests were 8.55, 8.01 and 6.15 t/ha, respectively. All varieties produced more leaves than stems with average leaf:stem ratios of 5, 5.9 and 4.8 for MM, BT and LC, respectively. In terms of CP production, MM was the highest yielder (272 kg/ha/harvest), followed by BT and LC (238 and 184 kg/ha/harvest, respectively). The total accumulative CP amounts over the 8 harvests were 2179, 1903 and 1474 kg/ha for MM, BT and LC, respectively. The mortality rates were 9.91, 14.01 and 13.98% for MM, BT and LC, respectively. Phosphorus content was more stable than potassium content during defoliation. MM, BT and LC had whole plant phosphorus contents of 0.41, 0.41 and 0.39%, respectively; whole plant potassium contents were 1.25, 1.38 and 1.20%.

• 한국수산과학회지
• /
• 제49권3호
• /
• pp.323-329
• /
• 2016

The effects of dietary antioxidant [saltwort (SW), leek (LK), and dandelion (DD)] supplementation on the growth, body composition, serum chemistry, and challenge test results of juvenile rockfish, Sebastes schlegelii, were determined. In an experiment, 320 fish were randomly distributed into eight, 50-L flow-through tanks (40 fish per tank). Four experimental diets were prepared: the control diet (Con) with synthetic antioxidant: and diets with SW, LK, or DD from natural sources. Each diet was randomly assigned to duplicate tanks of fish, which were hand-fed to satiation twice daily for 8 weeks. After the 8-week feeding trial, 20 fish from each tank were artificially infected with Streptococcus iniae and monitored for 6 days. The dietary additives did not affect survival, weight gain, specific growth rate, feed efficiency (FE), protein efficiency ratio (PER), or plasma chemistry. However, the cumulative mortality of fish fed the SW, LK and DD diets was lower than that of fish fed the control diet beginning 4 days after infection. In conclusion, dietary supplementation with SW, LK, and DD did not affect the growth, FE, PER, or plasma chemistry of rockfish. However, dietary inclusion of SW, LK, and DD lowered the mortality of rockfish following infection with S. iniae.

• ALGAE
• /
• 제36권1호
• /
• pp.25-36
• /
• 2021

The mixotrophic dinoflagellate Tripos furca causes red tides in the waters of many countries. To understand its population dynamics, mortality due to predation as well as growth rate should be assessed. Prior to the present study, the heterotrophic dinoflagellates Noctiluca scintillans, Polykrikos kofoidii, Protoperidinium steinii, and mixotrophic dinoflagellate Fragilidium subglobosum were known to ingest T. furca. However, if other common heterotrophic protists are able to feed on T. furca has not been tested. We explored interactions between T. furca and nine heterotrophic dinoflagellates and one naked ciliate. Furthermore, we investigated the abundance of T. furca and common heterotrophic protists in coastal-offshore waters off Yeosu, southern Korea, on Jul 31, 2020, during its red tide. Among the tested heterotrophic protists, the heterotrophic dinoflagellates Aduncodinium glandula, Luciella masanensis, and Pfiesteria piscicida were able to feed on T. furca. However, the heterotrophic dinoflagellates Gyrodiniellum shiwhaense, Gyrodinium dominans, Gyrodinium jinhaense, Gyrodinium moestrupii, Oblea rotunda, Oxyrrhis marina, and the naked ciliate Rimostrombidium sp. were unable to feed on it. However, T. furca did not support the growth of A. glandula, L. masanensis, or P. piscicida. Red tides dominated by T. furca prevailed in the South Sea of Korea from Jun 30 to Sep 5, 2020. The maximum abundance of heterotrophic dinoflagellates in the waters off Yeosu on Jul 31, 2020, was as low as 5.0 cells mL-1, and A. glandula, L. masanensis, and P. piscicida were not detected. Furthermore, the abundances of the known predators F. subglobosum, N. scintillans, P. kofoidii, and Protoperidinium spp. were very low or negligible. Therefore, no or low abundance of effective predators might be partially responsible for the long duration of the T. furca red tides in the South Sea of Korea in 2020.

• 환경생물
• /
• 제30권1호
• /
• pp.9-14
• /
• 2012

넓은 범위의 염분에 대한 망둑어 3종의 적응성을 파악하기 위하여 이들의 생존 및 성장에 대한 염분농도의 영향을 검토하였다. 두줄망둑은 0~33.6‰의 염분범위에서 유의한 생존율 차이가 없었고, 점망둑의 생존율은 염분농도 13.4‰ 이상에서 90% 이상을 나타내었으며, 3.4‰ 이하에서는 80% 이하로 유의하게 감소하였다. 또한, 날개망둑의 생존율은 염분농도 3.4‰ 이상에서 90% 이상을 나타내었으나, 담수에서는 87.5%로 유의한 감소가 관찰되었다. 두줄망둑은 염분농도 13.4 및 20.2‰에서 가장 높은 일일성장률은 나타냈고, 담수에서 유의한 감소가 관찰되었다. 점망둑의 일일성장률은 염분농도 20.2~33.6‰ 이상에서 가장 높았고, 10.1‰ 이하에서 가장 낮아 이들 사이의 유의한 차이를 나타냈다. 날개망둑의 일일성장률은 염분농도 10.1~27.4‰에서 가장 높았으나, 담수에서 유의한 감소가 나타났다. 이상의 결과는 3종의 망둑어, 특히 점망둑은 낮은 염분에 노출되었을 때, 그들의 생존 및 성장에 영향을 받는다는 것을 의미하고 있어 이들의 분포 및 개체수에 잠재적인 영향을 받을 수 있을 것으로 예상된다.