• Journal of Marine Bioscience and Biotechnology
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• v.10 no.2
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• pp.62-72
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• 2018

An active ingredient with hyaluronidase (HAse) inhibitory effect is one of the anti-aging approaches in cosmeceuticals. Here, red sea cucumbers (RSCs), Stichopus japonicus, from Jeju Island were evaluated to examine their HAse inhibitory and antioxidant activity effect. In this study, RSCs were extracted by six enzymatic hydrolysis (Alcalase; Al, Trypsin; Try, Neutrase; Neu, Pepsin; Pep, Alpha-chymotrypsin; Chy and Protamex; Pro). Alcalase hydrolysate (AlH) showed the highest antioxidant capacities for both of oxygen radical absorbance capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) methods, compared to those of other hydrolysates, at $66.59{\pm}0.78{\mu}M\;TE/mg$ and $135.78{\pm}3.24{\mu}M\;TE/mg$, respectively. Furthermore, AlH performed the highest capacity of HAse inhibitory with $IC_{50}$ value of 3.21 mg/ml. Thus, RSCs hydrolyzed by Al were chosen to determine the cellular antioxidant activity and hyaluronic acid (HA) production effect on Human immortalized keratinocyte cell line (HaCaT). The results showed that AlH improved the cell viabilities and intracellular reactive oxygen species (ROS) induced by 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) were significantly decreased. In addition, AlH increased HA amount by regulating HYAL2 and HAS2 expressions in the HaCaT cells. Taken together, AlH of RSCs collected from Jeju Island showed HAse inhibitory and antioxidant activities against skin-aging which shows its potentials can be an optional natural bioactive ingredient for novel cosmeceuticals.

• Food Science and Industry
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• v.52 no.4
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• pp.358-374
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• 2019

Indigestible carbohydrates as dietary fiber have attracted interest of consumers due to their several physiological benefits. Recent definitions of dietary fiber have included other indigestible carbohydrates such as resistant starch and resistant maltodextrins, which are natural, colorless, odorless and tasteless low-calorie food ingredients. Unlike some carbohydrates and digestible starches, indigestible starch and maltodextrin resist enzymatic hydrolysis in the upper gastrointestinal tract, resulting in little or no direct glucose absorption. In addition, there is increased microbial fermentation production of short-chain fatty acids in the large intestine. As an emerging functional low-calorie food ingredient, resistant starch and maltodextrin have been shown to have equivalent or superior impacts on human health compared to conventional fiber-enriched food ingredients. In this paper, the definition, strategies to enhance dietary fiber content in foods, some potential health benefits, and applications in food industry for indigestible starch and maltodextrin are summarized and discussed.

• Journal of the Korean Applied Science and Technology
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• v.40 no.6
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• pp.1373-1380
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• 2023

This study investigated the effects of coumarin, a flavonoid known for various physiological activities like antiviral, anticancer, and antibacterial properties, on anti-oxidants and anti-inflammatory processes, aiming to explore its application in functional cosmetics. The results are as follows: Cell toxicity experiments using RAW 264.7 cells showed no significant cytotoxicity for coumarin at any concentration, indicating its safety for skin application. Observing coumarin's antioxidant activity through DPPH radical scavenging showed concentration-dependent effectiveness, though not significantly varied with concentration. The inhibition of silica-induced ROS production in cells was concentration-dependent. Both NO production inhibition and histamine release measurements showed concentration-dependent suppression. These findings suggest that Coumarin can be effectively used as a natural ingredient in cosmetic development for its antioxidant and anti-inflammatory properties.

• Herbal Formula Science
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• v.32 no.1
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• pp.83-90
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• 2024

Objectives : As a substitute for high-price ginseng, this study attempted to examine a possibility of the ferment extract of Panax ginseng sprout whether leaves and roots can be used together as a cosmetic ingredient with anti-oxidative and wrinkle-care effects. Methods : In terms of a test method, antioxidant activities were confirmed through total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity and ABTS radical scavenging activity using the Panax ginseng sprout. In addition, to assess wrinkle-care effectiveness, the cytotoxicity of the extract was analyzed through MTT assay, and inhibition of collagenase activity in the cells was tested using the Panax ginseng sprout fermented by Saccharomyces cerevisiae. Resuits : The content of polyphenols and flavonoids in natural plants was highest in Panax Ginseng Sprout Extract at 100℃, which also demonstrated high DPPH, ABTS radical scavenging activity. MTT assay demonstrated that the Panax Ginseng Sprout Ferment Extract did not have a cytotoxic effect in CCD-986SK cell. Also, Panax Ginseng Sprout Ferment Extract was found to inhibit MMP-1 expression by 51.85±6.09% at a concentration of 10%. Conclusions : Therefore, this study has confirmed a possibility of Panax ginseng sprout ferment extract as a cosmetic ingredient with MMP-1-inhibitory effects.

• The Korea Journal of Herbology
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• v.26 no.2
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• pp.67-73
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• 2011

Objectives : The purpose of this research was total polyphenol contents, anti-oxidant activities, anti-inflammatory activities and anti-wrinkle activities of Ulleung islands plants for application as a cosmeceutical ingredients Methods : We were experimented total polyphenol contents, anti-oxidant activities, anti-inflammatory activity and anti-wrinkle activities. Results : In the physiological activities, most Ulleung islands plants is showed the highest anti-oxidant, anti-inflammatory activity in ABTS+ radical cation scavenging activity, hydrogen peroxide($H_2O_2$) scavenging activity, nitric oxide scavenging activity. All experiment of the water and ethanol extract from the Ulleung islands plants ingredients were gradually increased as well. Conclusions : These results suggest that extracts from Ulleung islands plants can be used in natural ingredient in food or cosmetic industry.

• Carbon letters
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• v.4 no.2
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• pp.79-85
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• 2003

MCMB (mesocarbon microbeads) has been synthesized from coal tar pitch, petroleum pitch and polymer compound generally. But yield of MCMB was low about 20~40 wt% and was not above 50 wt%. Neither MCMB was replaced with natural graphite because of economic performance, refining MCMB, and control of the particle size distribution. This study was performed to elevate yield of MCMB and to develop technique of particle size distribution. As the result, yield of MCMB that was synthesized from coal tar pitch increased more than 60 wt% about raw material and particle size of MCMB was restrained according to control of QI (quinoline insoluble) ingredient in raw pitch, heat treatment temperature and time.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2004.10a
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• pp.36-38
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• 2004

Ethaboxam is a new fungicidal active ingredient that inhibits growth of plant pathogens specifically belonging to Oomycetes with protective, curative, systemic and translaminar activity in plants. Modes of action studies revealed that ethaboxam simultaneously inhibits cytoskeleton formation and mitochondrial respiration of Phytophthora infestans at low concentrations. There have been no indications of resistance development when tested for baseline resistance monitoring to 261 isolates of P. infestans in Korea and Europe and 150 populations of Plasmopara viticola populations in Europe for 3 years since 2000. In a selective study with vine trees artificially inoculated with P. viticola repeatedly for 10 generations in greenhouse, there have been no changes in sensitivity to ethaboxam among four natural populations of P. viticola. Furthermore, ethamoxam has not shown any cross resistance with azoxystrobin, mefenoxam, dimethomorph and cymoxanil. Based on the study results from modes of action and resistance development, ethaboxam appears to be unlikely to develop resistance in field applications.

• Annals of Clinical Neurophysiology
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• v.3 no.1
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• pp.50-52
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• 2001

Licorice is widely used as a Chinese(herbal) medicine. The glycyrrhizin, a main ingredient of the natural licorice, has a potent mineralocorticoid effect which may cause severe hypokalemia and muscle paralysis. We present a 60-year-old woman, who had been ingesting one or two spoonful of licorice powder daily for about one year, developed acute flaccid quadriparesis with high levels of serum muscle enzymes and the typical features of mineralocorticoid excess such as severe hypokalemia and metabolic alkalosis. Both plasma renin activity and serum aldosterone level were below the normal values. This case indicates that licorice-induced hypokalemic myopathy should be considered in the differential diagnosis of a patient with acute quadriparesis and hypokalemia.

• YAKHAK HOEJI
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• v.54 no.4
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• pp.240-243
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• 2010

High-performance liquid chromatography (HPLC) screening method revealed that a propolis product marketed as a functional food contained an undeclared substance similar to tadalafil, the active ingredient of the prescription drug Cialis$^{(R)}$ approved for the treatment of male erectile dysfunction. In order to identify the illegal additive, the propolis product was extracted with methylene chloride, and the extract was purified further using semipreparative HPLC. The chemical structure of the isolated substance was elucidated based on IR, LC/MS-ESI, and $^1H$- and $^{13}C$-NMR spectroscopy, which showed the characteristics similar to tadalafil. The only difference was the substitution of the methyl group at the piperazinedione ring of tadalafil to the amino group of the identified illegal additive.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4301-4305
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• 2014

Tanshinone IIA is a pharmacologically active ingredient extracted from Danshen, a Chinese traditional medicine. Its molecular mechanisms are still unclear. The present study utilized computational approaches to uncover the potential targets of this compound. In this research, PharmMapper server was used as the inverse docking tool andnd the results were verified by Autodock vina in PyRx 0.8, and by DRAR-CPI, a server for drug repositioning via the chemical-protein interactome. Results showed that the retinoic acid receptor alpha ($RAR{\alpha}$), a target protein in acute promyelocytic leukemia (APL), was in the top rank, with a pharmacophore model matching well the molecular features of Tanshinone IIA. Moreover, molecular docking and drug repurposing results showed that the complex was also matched in terms of structure and chemical-protein interactions. These results indicated that $RAR{\alpha}$ may be a potential target of Tanshinone IIA for APL. The study can provide useful information for further biological and biochemical research on natural compounds.