• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.644-651
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• 2013

The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormone-like activity. The material was found to harbor a bacterial community of $2.38{\times}10^8$ CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of ${\beta}$-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.5
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• pp.311-320
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• 2017

Suppression of sloshing is essential to achieve fast transportation and stable maneuvering of tanks partially filled with liquid. In this study, numerical simulations are performed to investigate the effects of the acceleration magnitude and the acceleration duration of triangular velocity profiles on sloshing when a rectangular tank moves horizontally. We previously reported, based on only the first natural mode, that sloshing is significantly suppressed when the acceleration duration equals the first natural period of sloshing. On the other hand, the present CFD simulations find the best acceleration duration for minimum sloshing and explains the results considering higher modes as well as the first mode. We also perform the analysis using an equivalent model based on masses and springs, and evaluate its accuracy by comparing it with the CFD simulation results.

• Journal of Korean Society of Steel Construction
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• v.17 no.2 s.75
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• pp.173-181
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• 2005

This study deals with a method to identify damages of free vibrating thin plate structures using the combined finite element method (FEM) and the advanced uniform micro-genetic algorithm.To solve the inverse problem using the combined method, this study uses several natural frequencies instead of mode shapes in a structure as the measured data. The technique described in this paper allows us not only to detect the damaged elements but also to find their numbers, locations, and the extent of damage.To demonstrate the feasibility of the proposed method, the algorithm is applied to a free vibrating steel thin plate structures with arbitrary damages. From the standpoint of computation efficiency, the proposed method in this study has advantages when compared with the existing simple genetic algorithms. The numerical examples demonstrate that the method using micro-genetic algorithms can possibly detect correctly the damages of thin plates from only several natural frequencies instead of their natural modes.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1790-1799
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• 2016

Matrix assisted laser desorption ionization (MALDI)-time of flight/mass spectrometry (TOF/MS) was applied to investigate alterations in phospholipids in mitophagic cancer cells. Several phospholipids, including phosphatidylcholines (PCs), sphingomyelins (SMs), and phosphatidylinositols (PIs), were successfully analyzed in control and mitophagy-induced H460 cells in the positive and negative ion modes. Principal component analysis was applied to differentiate the two groups. The upregulated and downregulated phospholipid species in the mitophagic cells were also represented in a heatmap. In the volcano plot (fold change > 1.3 and p value < 0.01), individual species of seven PCs, two SMs, and three PIs were selected as differentially regulated phospholipids. In particular, almost all the molecular species of PC, SM, and PI were downregulated in the mitophagic cells. Quantification of these lipids indicated that mitophagy induces altered metabolism of phospholipids. Therefore, phospholipid alterations during the mitophagic process of lung cancer cells were well characterized by MALDI-TOF/MS.

• Structural Engineering and Mechanics
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• v.4 no.5
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• pp.553-568
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• 1996

Finite element stiffness matrix methods are presented for finding natural frequencies (or buckling loads) and modes of repetitive structures. The usual approximate finite element formulations are included, but more relevantly they also permit the use of 'exact finite elements', which account for distributed mass exactly by solving appropriate differential equations. A transcendental eigenvalue problem results, for which all the natural frequencies are found with certainty. The calculations are performed for a single repeating portion of a rotationally or linearly (in one, two or three directions) repetitive structure. The emphasis is on rotational periodicity, for which principal advantages include: any repeating portions can be connected together, not just adjacent ones; nodes can lie on, and members along, the axis of rotational periodicity; complex arithmetic is used for brevity of presentation and speed of computation; two types of rotationally periodic substructures can be used in a multi-level manner; multi-level non-periodic substructuring is permitted within the repeating portions of parent rotationally periodic structures or substructures and; all the substructuring is exact, i.e., the same answers are obtained whether or not substructuring is used. Numerical results are given for a rotationally periodic structure by using exact finite elements and two levels of rotationally periodic substructures. The solution time is about 500 times faster than if none of the rotational periodicity had been used. The solution time would have been about ten times faster still if the software used had included all the substructuring features presented.

• Magazine of the Korean Society of Agricultural Engineers
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• v.38 no.3
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• pp.50-57
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• 1996

A numerical method is presented to obtain the natural frequencies and mode shapes of the arbitrary tapered beams with static deflection due to arbitrary distributed dead loads. The differential equation governing free vibration of such beams is derived and solved numerically. The double integration method using the trapezoidal rule is used to solve the static behaviour of beams loaded arbitrary distributed dead load. Also, the Improved Euler method and the determinant search method are used to integrate the differential equation subjected to the boundary conditions and to determine the natural frequencies of the beams, respectively. In the numerical examples, the various geometries of the beams are considered : (1) linearly tapered beams as the arbitrary variable cross-section, (2) the triangular, sinusoidal and uniform loads as the arbitrary distributed dead loads and (3) the hinged-hinged, clamped-clamped and hinged-clamped ends as the end constraints. All numerical results are shown as the non-dimensional forms of the system parameters. The lowest three natural frequencies versus load parameter, slenderness ratio and section ratio are reported in figures. And for the comparison purpose, the typical mode shapes with and without the effects of static deflection are presented in the figure. According to the numerical results obtained in this analysis, the following conclusions may be drawn : (1) the natural frequencies increase when the effects of static deflections are included, (2) the effects are larger at the lower modes than the higher ones and (3) it should be betteF to include the effect of static deflection for calculating the frequencies when the beams are supported by both hinged ends or one hinged end.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.5
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• pp.2617-2622
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• 2014

In this study, the critical frequency happened at system is analyzed when the motor of camera manufacturing system is working on the direction of Z axis. Configurations of modes at natural frequencies happened at two models are investigated by the study result through modal analysis. The range of natural frequency in this study system is from 100 Hz to 500 Hz. At this range, the maximum equivalent stress in case of 20 kg weight becomes 6.2335MPa and this stress is shown as 50 times more than in case of 10 kg weight. The working safety of system can be investigated through the analyses of natural frequency and harmonic response of this camera manufacturing system.

• Structural Engineering and Mechanics
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• v.28 no.1
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• pp.53-67
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• 2008

Anisotropic materials are increasingly required in modern technological applications. Certainly, civil, mechanical and naval engineers frequently deal with the situation of analyzing the dynamical behaviour of structural elements being composed of such materials. For example, panels of anisotropic materials must sometimes support electromechanical engines, and besides, holes are performed in them for operational reasons e.g., conduits, ducts or electrical connections. This study is concerned with the natural frequencies and normal modes of vibration of rectangular anisotropic plates supported by different combinations of the classical boundary conditions: clamped, simply - supported and free, and with additional complexities such holes of free boundaries and attached concentrated masses. A variational approach (the well known Ritz method) is used, where the displacement amplitude is approximated by a set of beam functions in each coordinate direction corresponding to the sides of the rectangular plate. Consequently each coordinate function satisfies the essential boundary conditions at the outer edge of the plate. The influence of the position and magnitude of both hole and mass, on the natural frequencies and modal shapes of vibration are studied for a generic anisotropic material. The classical Ritz method with beam functions as spatial approximation proved to be a suitable procedure to solve a problem of such analytical complexity.

• Smart Structures and Systems
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• v.16 no.5
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• pp.937-960
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• 2015

Tuned mass dampers (TMDs) have been a prevalent vibration control device for suppressing excessive vibration because of environmental loadings in contemporary tall buildings since the mid-1970s. A TMD must be tuned to the natural frequency of the primary structure to be effective. In practice, a TMD may be assembled in situ, simultaneously with the building construction. In such a situation, the respective dynamic properties of the TMD device and building cannot be identified to determine the tuning status of the TMD. For this purpose, a methodology was developed to obtain the parameters of the TMD and primary building on the basis of the eigenparameters of any two complex modes of the combined building-TMD system. The theory was derived in state-space to characterize the nonclassical damping feature of the system, and combined with a system identification technique to obtain the system eigenparameters using the acceleration measurements. The proposed procedure was first demonstrated using a numerical verification and then applied to real, experimental data of a large-scale building-TMD system. The results showed that the procedure is capable of identifying the respective parameters of the TMD and primary structure and is applicable in real implementations by using only the acceleration response measurements of the TMD and its located floor.

• Korean Journal of Environmental Biology
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• v.21 no.3
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• pp.303-307
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• 2003

Tributyltin (TBT) used world-wide in antifouling paints toy ships is a wide-spread environmental pollutant. At low doses, antiproliferative modes of action have been shown to be involved, whereas at higher doses apoptosis seems to be the mechanism of toxicity in reproductive organs by TBT. In this study, we investigated that the mechanisms underlying apoptosis induced by TBT in R2C cell. Effects of TBT on intracellular $Ca^{2+}$ level and reactive oxygen species (ROS) were investigated in R2C cells by fluorescence detector. TBT significantly induced intracellular $Ca^{2+}$ level in a time-dependent manner. The rise in intracellular $Ca^{2+}$ level was followed by a time-dependent generation of reactive oxygen species (ROS) at the cytosol level. Simultaneously, TBT induced the release of cytochrome c from the mitochondrial membrane into the cytosol. Furthermore, ROS production and the release of cytochrome c were reduced by BAPTA, an intracellular $Ca^{2+}$ chelator, indicating the important role of $Ca^{2+}$ in R2C during these early intracellular events. In addition, Z-DEVD FMB, a caspase -3 inhibitor, decreased apoptosis by TBT. Taken together, the present results indicated that the apoptotic pathway by TBT might start with an increase in intracellular $Ca^{2+}$ level, continues with release of ROS and cytochrome c from mitochondria, activation of caspases, and finally results in DNA fragmentation.