• Food Science and Preservation
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• v.14 no.2
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• pp.183-187
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• 2007

The antimicrobial properties of methanolic extract (PML) from Prunus nune byproducts after liquor manufacturing weremeasured using various putrefactive and food spoilage microorganisms. PML showed remarkable antimicrobial effects against various putrefactive and food spoliage microorganisms when used at 500g/mL. The antimicrobial properties were stable for 30 min at 100C and at pH 3 11. PML seems to be a natural antimicrobial agent with high effectiveness, and shows both thernal and pH stability. In addition, the mode of antimicrobial action suggests that the active components may synergistically perturb microbial membrane functions.

• Smart Structures and Systems
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• v.19 no.1
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• pp.39-46
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• 2017

Finite element model updating is very effective procedure to determine the uncertainty parameters in structural model and minimize the differences between experimentally and numerically identified dynamic characteristics. This procedure can be practiced with manual and automatic model updating procedures. The manual model updating involves manual changes of geometry and analyses parameters by trial and error, guided by engineering judgement. Besides, the automated updating is performed by constructing a series of loops based on optimization procedures. This paper addresses the ambient vibration based finite element model updating of long span reinforced concrete highway bridges using manual model updating procedure. Birecik Highway Bridge located on the $81^{st}km$ of Şanliurfa-Gaziantep state highway over Firat River in Turkey is selected as a case study. The structural carrier system of the bridge consists of two main parts: Arch and Beam Compartments. In this part of the paper, the arch compartment is investigated. Three dimensional finite element model of the arch compartment of the bridge is constructed using SAP2000 software to determine the dynamic characteristics, numerically. Operational Modal Analysis method is used to extract dynamic characteristics using Enhanced Frequency Domain Decomposition method. Numerically and experimentally identified dynamic characteristics are compared with each other and finite element model of the arch compartment of the bridge is updated manually by changing some uncertain parameters such as section properties, damages, boundary conditions and material properties to reduce the difference between the results. It is demonstrated that the ambient vibration measurements are enough to identify the most significant modes of long span highway bridges. Maximum differences between the natural frequencies are reduced averagely from %49.1 to %0.6 by model updating. Also, a good harmony is found between mode shapes after finite element model updating.

• Journal of the Korea Society of Computer and Information
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• v.26 no.10
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• pp.9-17
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• 2021

Humans are social animals that have gained information or social interaction through dialogue. In conversation, the mood of the word can change depending on the sensibility of one person to another. Relationships between characters in films are essential for understanding stories and lines between characters, but methods to extract this information from films have not been investigated. Therefore, we need a model that automatically analyzes the relationship aspects in the movie. In this paper, we propose a method to analyze the relationship between characters in the movie by utilizing deep learning techniques to measure the emotion of each character pair. The proposed method first extracts main characters from the movie script and finds the dialogue between the main characters. Then, to analyze the relationship between the main characters, it performs a sentiment analysis, weights them according to the positions of the metabolites in the entire time intervals and gathers their scores. Experimental results with real data sets demonstrate that the proposed scheme is able to effectively measure the emotional relationship between the main characters.

• Journal of Marine Bioscience and Biotechnology
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• v.13 no.2
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• pp.94-103
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• 2021

Here, we evaluated the anti-glycation effects and renal protective properties of 70% (v/v) ethanolic extract of Colpomenia sinuosa (CSE) against AGEs -induced oxidative stress and apoptosis at different concentrations (1, 5, and 20 ㎍/mL). At 20 ㎍/mL, CSE showed that anti-glycation activities via the inhibition of AGE formation (51.1%), inhibition of AGEs-protein cross-linking (61.7%), and breaking of AGEs-protein cross-links (33.3%), were significantly (###p < 0.001 vs. non-treated group) lower than the nontreated group. Methylglyoxal (MGO) significantly (***p < 0.001) reduced cell viability (24.4%) and increased reactive oxygen species (ROS) level (642.3%), MGO accumulation (119.4 ㎍/mL), and apoptosis (55.0%) in mesangial cells compared to the nontreated group. Pretreatment with CSE significantly (###p < 0.001) increased cell viability (57.8%) and decreased intracellular ROS (96.5%), MGO accumulation (80.0 ㎍/mL), and apoptosis (22.6%) at 20 ㎍/mL. Additionally, we confirmed intracellular AGEs reduction by CSE pretreatment. Consequently, our results suggest that CSE is a good source of natural therapeutics for managing diabetic complications by the antiglycation effect and renal protective activity against MGO-induced oxidative stress.

• Korean Journal of Pharmacognosy
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• v.52 no.4
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• pp.242-250
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• 2021

Artemisia frigida Willd (AW, Fringed sagewort), which is widespread in Mongolia, is a well-known medicinal plant as a member of the Compositae family. This study aims to explore the gastroprotective effect of water extract of AW on 150 mM HCl/60% ethanol-induced acute gastritis in 5 week old male ICR mice. Total polyphenols, total flavonoid contents, and anti-oxidant activity in vitro in AW were evaluated. First, the gross area of gastric mucosal damage was measured. Then western blot analysis was conducted to determine the possible mechanisms of action underlying the effects of AW. AW administration decreased gastric mucosal damage. Moreover, the group with AW treatment effectively inhibited nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expression associated with oxidative stress. AW treatment enhanced an anti-oxidant effect through the increase of anti-oxidant proteins. Besides, the increased expressions of inflammatory cytokines induced by nuclear factor-kappa B (NF-κB) activation are alleviated through AW treatment. Taken together, AW exerted a gastroprotective effect against gastric mucosal damage. These results indicate that AW could have the potential used as a natural therapeutic drug for the treatment of acute gastritis.

• The Korean Journal of Food And Nutrition
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• v.34 no.5
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• pp.423-429
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• 2021

Barley's nutritional value as a health food is increasing due to its excellent nutritional functionality. In this study, the levels of β-glucan, total polyphenols, and total flavonoids were analyzed in the ethanol extracts of different barley cultivars (Hinchalssal, Heuksoojeongchal, Betaone, Ganghochung, and Saechalssal). Also, the free radical scavenging abilities of 2,2-diphenyl-1-picryl-hydrazil (DPPH) and 2,2'-azino-bis-3-ethylbenzo-thiaxoline-6-sulfonic acid (ABTS) were measured to determine their antioxidant activity. The results confirmed that Betaone extract contained highly activefunctional components and exhibitedantioxidant activity. Next, we evaluated the hepatoprotective and inhibitory effects of reactive oxygen species (ROS) generated by barley ethanol extracts after inducing oxidative stress with tert-butyl hydroperoxide (tBHP) in HepG2 cells. Hinchalssal and Saechalssal extracts showed the most significant cytoprotective effect and also reduced ROS production significantly. These results suggest that Hinchalssal, Saechalssal, and Betaone represent potential natural antioxidant and hepatoprotective agents.

• Natural Product Sciences
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• v.27 no.3
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• pp.187-192
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• 2021

Cordycepin is a characteristic bioactive compound of Cordyceps militaris with various beneficial effects. Cordyceps grows on both grains and insects, and the content of cordycepin varies depending on the cultivation conditions. In this study, the effect of culture conditions on the cordycepin content was analyzed and the extraction conditions were optimized. Analysis of cordycepin content in Pupae-Cordyceps found that it was highly affected by temperature in culture conditions. In the case of mycelium, it grows well at 20 and 25 ℃, but not at 30 ℃. However, the content of cordycepin was highest at 30℃ and less at 20 ℃. The fruiting body also showed a similar tendency: growth was 20 ℃ > 25 ℃ > 30 ℃, but the cordycepin content was 30 ℃ > 25 ℃ > 20 ℃. The content of cordycepin decreased after the fruiting bodies were produced. Next, extraction conditions such as solvent and time were optimized for maximum cordycepin content using response surface methodology (RSM). There was a large difference in the content of cordycepin according to the content of ethanol and the extraction temperature. Through RSM, it was confirmed that the optimum condition for extraction of cordycepin was 48.9 ℃ using 49.0% ethanol, and 160.9 mg/g extract could be obtained under this condition. In conclusion, this study suggested the optimized conditions for the cultivation and extraction of Pupae-Cordyceps for maximizing the content of cordycepin, and this may be applied to the discovery of materials using cordycepin.

• Journal of Forest and Environmental Science
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• v.37 no.3
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• pp.226-234
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• 2021

Two species, P. pyrifolia and P. ussuriensis, of the genus Pyrus native to Korea, are valuable genetic resources that can be used for food, dietary supplement, pharmaceutical, and cosmetics. Bioactive compounds of the plant leaves are the main components that are used for the products. Farmers had cultivated a few individuals from the wild to produce fruits or leaves for traditional remedy or tea; however, bioactive components of their leaves are not tested. We selected some trees from the natural stand that have distinct traits for the improvement program. We investigated the bioassay on the extracts' bioactive compounds and antioxidant capacity from the selected accessions and other accessions, including newly developed cultivars. The contents of the phenolic compounds and flavonoids from the leaf extracts of the selected accessions were higher than the commonly cultivated trees in both species but lower than 'Sanhyang' in P. ussuriensis. The antioxidant capacity was measured using two assay methods, DPPH and ABST. The selected cultivars also had higher inhibitory activity than common trees. The selected accession 'Cultivar 3' in P. pyrifolia had the highest radical scavenging activity than others. Although leaves of the accessions were used only in this study, all three selected individuals have the potential for cultivar in containing high bioactive compounds and antioxidant capacity.

• Natural Product Sciences
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• v.27 no.3
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• pp.201-207
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• 2021

We have previously reported that Acer tegmentosum extract, which is traditionally used in Korea to reduce alcohol-related liver injury, suppresses liver inflammation caused by excessive alcohol consumption and might improve metabolism. The active ingredient, 6-O-galloylsalidroside (GAL), was isolated from A. tegmentosum, and we hypothesized that GAL could provide desirable pharmacological benefits by ameliorating physiological conditions caused by alcohol abuse. Therefore, this study focused on whether GAL could ameliorate alcoholic fat accumulation and repair liver injury in mice. During chronic alcohol consumption plus binge feeding in mice, GAL was administered orally once per day for 11 days. Intrahepatic lipid accumulation was measured in vivo using a noninvasive method, ¹H magnetic resonance imaging, and confirmed by staining with hematoxylin and eosin and Oil Red O. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using a Konelab system, and the triglyceride content was measured in liver homogenates using an enzymatic peroxide assay. The results suggested that GAL alleviated alcohol-induced steatosis,e as indicated by decreased hepatic and serum triglyceride levels in ethanol-fed mice. GAL treatment also correlated with a decrease in the Cd36 mRNA expression, thus potentially inhibiting the development of alcoholic steatosis via the hepatic de novo lipogenesis pathway. Furthermore, treatment with GAL inhibited the expression of cytochrome P450 2E1 and attenuated hepatocellular damage, as reflected by a reduction in ALT and AST levels. These findings suggest that GAL extracted from A. tegmentosum has the potential to serve as a bioactive agent for the treatment of alcoholic fatty liver and liver damage.

• Journal of the Korea Society of Computer and Information
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• v.26 no.11
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• pp.33-40
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• 2021

Due to advanced complex strategies, the complexity of information that a commander must analyze is increasing. An intelligent service that can analyze battlefield is needed for the commander's timely judgment. This service consists of extracting knowledge from battlefield information, building a knowledge base, and analyzing the battlefield information from the knowledge base. This paper extract information similar to an input query by embedding the knowledge base built in the 2^nd step. The transformation model is needed to generate the embedded knowledge base and uses the random-walk algorithm. The transformed information is embedding using Word2Vec, and Similar information is extracted through cosine similarity. In this paper, 980 sentences are generated from the open knowledge base and embedded as a 100-dimensional vector and it was confirmed that similar entities were extracted through cosine similarity.