The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidative and Hepatoprotective Effects of Ethanol Extracts from Different Barley Cultivars

보리 품종별 주정 추출물의 항산화 활성 및 간 보호 효과

  • Yang, Ji Yeong (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Ham, Hyeonmi (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Hyun-Jin (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Hyun Young (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Woo, So-Yeun (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Seo, Woo Duck (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Mi Ja (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
  • 양지영 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 함현미 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이현진 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김현영 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 우소연 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 서우덕 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이미자 (농촌진흥청 국립식량과학원 작물기초기반과)
  • Received : 2021.07.22
  • Accepted : 2021.09.06
  • Published : 2021.10.31
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Abstract

Barley's nutritional value as a health food is increasing due to its excellent nutritional functionality. In this study, the levels of β-glucan, total polyphenols, and total flavonoids were analyzed in the ethanol extracts of different barley cultivars (Hinchalssal, Heuksoojeongchal, Betaone, Ganghochung, and Saechalssal). Also, the free radical scavenging abilities of 2,2-diphenyl-1-picryl-hydrazil (DPPH) and 2,2'-azino-bis-3-ethylbenzo-thiaxoline-6-sulfonic acid (ABTS) were measured to determine their antioxidant activity. The results confirmed that Betaone extract contained highly activefunctional components and exhibitedantioxidant activity. Next, we evaluated the hepatoprotective and inhibitory effects of reactive oxygen species (ROS) generated by barley ethanol extracts after inducing oxidative stress with tert-butyl hydroperoxide (tBHP) in HepG2 cells. Hinchalssal and Saechalssal extracts showed the most significant cytoprotective effect and also reduced ROS production significantly. These results suggest that Hinchalssal, Saechalssal, and Betaone represent potential natural antioxidant and hepatoprotective agents.

Keywords

Acknowledgement

본 연구는 농촌진흥청 AGENDA 연구사업(ATIS 과제번호: PJ0135242021) 및 농촌진흥청 국립식량과학원 전문연구원 과정 지원 사업에 의해 이루어진 것임.

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