• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.387-394
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• 2009

This study was carried out to screen and identify the chitinase and chitosanase producing microorganisms from the feces of calves medicated DFM sincluding chitin in order to do the immune fortification of Korean Native calves. Ten isolates were grown in the medium containing chitin and chitosan that had more than $10^5$ cfu/g in feces. Among these 10 strains, 2 strains (HANDI 110 and HANDI 309) had the chitinase activities and 2 strains (HANWOO and HANYOO) had the chitosanase activities in calves' feces. They showed no reaction in hemolysis tests by utilizing chitin and chitosan. The results from morphological, physicochemical and genetical identification indicated the HANDI 110 as a strain of Escherichia fergusonii, HANDI 309 was identified as a strain of Acinetobacter parvus, HANWOO was identified as a strain of Comamonas koreensis, and HANYOO as a strain of Chryseobacterium indologenes.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.623-623
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• 2013

그래핀(graphene)의 라만 스펙트럼은 전하밀도(charge density)와 기계적 변형(strain)에 민감하여 수많은 연구에 활용되고 있다. 그러나 실제 시료에서 관찰되는 두 물질량의 복잡한 변이를 정량 분석하기 위해서는 기계적 변형뿐만 아니라 전하밀도의 영향에 대한 신뢰도 높은 검정곡선이 필요하다. 본 연구에서는 기계적 박리법으로 만들어진 그래핀에서 나타나는 기계적 변형과 황산 수용액이 미치는 p-형 화학도핑(chemical doping)의 영향을 라만 분광법을 이용하여 연구하였다. 농도 변화에 따른 G와 2D 피크의 진동수 변화는 정전기적 방법을 이용하여 보고된 결과보다 높은 재현성을 보여 검정곡선으로 활용되기에 적합함을 알 수 있었다. 본 연구에서는 514 nm 이외에도 널리 활용되는 몇 가지 파장에서 "전하밀도-변형" 검정곡선을 제시하고자한다.

• Molecules and Cells
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• v.28 no.4
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• pp.369-373
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• 2009

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and ${\beta}$-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an ${\alpha}$-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and ${\beta}$-glucosidase was able to produce ethanol from ${\beta}$-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

• Journal of Life Science
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• v.30 no.11
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• pp.1007-1011
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• 2020

This study was conducted to analyze the genetic diversity and relationships that discriminate between Korean native black goat populations (Dangjin, Jangsu, Tongyoung, and Gyeongsang National University strains) and crossbred goats. Monomorphic single nucleotide polymorphisms (SNPs) in each strain were collected, and 133 common SNPs were selected for analysis. These 133 monomorphic SNPs showed differences in the genetic structure of the Korean native black goat and crossbred goats, and results from the principal component analysis (PCA) showed that the two can be clearly separated. Furthermore, analysis of the validation population comprising 70 individuals (Korean native black goats, n = 24; crossbred goats, n = 46) with the reference population showed that Korean native black goat strains and the reference population have the same genetic structure, and the crossbred goats shared only part of the genetic structure with the reference population. The result of the PCA analysis showed that the Korean native black goat strains form one population, whereas the foreign strains form another population which is more widely dispersed than the Korean native black goat strains. Thus, the results from this study can be used as baseline data for the conservation of genetic resources of Korean native black goat communities through utilization of monomorphic SNPs and for the introduction of exotic species for further improvement in genetic diversity. This study can also help reduce unnecessary inbreeding and gene flow between native strains.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.37 no.3
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• pp.66-73
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• 2005

This study was performed to understand the changes in wood extractives, mainly acetone extracts, in pine woods (Pinus densiflora and Pinus rigida) treated by three blue stain fungi (BSF) such as native BSF in Korea, Leptographium sp., screened Albino strain(BSFcs-1) and commercial Cartapip and fungicide, Wood guard. In addition their pulping and bleaching properties were investigated. BSF treatment has significantly reduced acetone extracts, $25.1{\sim}30.4%$ decreasing in red pine and $22.9{\sim}28.1%$ in pitch pine. Three week aging treatment showed about 20% decreasing in red pine and 19.3% in pitch pine. There were not so significant differences in extracts reduction among native BSF and Albino-type strains (Albino strain, BSFcs-1, and commercial Cartapip). But fungicide, Wood guard, treated wood showed relatively lower decreasing rates of extractives, 14% in red pine and 10.1% in pitch pine. Therefore it is understandable that the fungicide could protect the wood from blue stain fungi attack, but has no effect on its extractive reduction. Concerned to pulping properties of BSF and fungicide treated woods, red pine and pitch pine, optimum pulping condition was 20% active alkali, wood to liquor ratio 1 to 6, $170^{\circ}C$, and 2.5 hr. In the case of BSF woods, optimum pulping condition was same as the sound wood, $43.5{\sim}45%$ of pulp yields and $1.3{\sim}1.45%$ of rejects. Screened pulp yield of fungicide treated wood was lower than those of BSF treated woods. Rejects in pulps were higher in fungicide-treated wood than BSF treated woods. Bleaching pulp yields were ranged of 92 to 93.5%. BSF, Cartapip and fungicide treated woods resulted in lower brightness of $55{\sim}58%$, but Albino-type strain(BSFcs-1) $61.3{\sim}62.3%$, very similar to untreated one. Therefore bleaching chemicals could be saved in the processing of chemical pulping.

• Korean Journal of Poultry Science
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• v.30 no.4
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• pp.235-244
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• 2003

This study was carried out to estimate the effects of strain and generation on the major production performances of Korean native chicken. The data were collected from 11,583 birds of the 7 generations over the year 1995 through 2001 at National Livestock Research Institute, Korea. Results obtained were summarized as follows: The body weights at 150 and 270 days of age were 1,557.7 and 1880.7, 1,471.7 and 1,738.2, 1,393.5 and 1,694.9, 1,591.2 and 1,910.0, 1,545.6 and 1,763.6g in Red Brown, Yellow Brown, Gray Brown, Black, and White strains, respectively. The Coefficient of Variations (CVs) of the body weights at 150 and 270 days of age were 9.65~13.79% and 13.29~15.16%. The ages at first egg were 150.0, 148.3, 149.5, 152,8 and 147.7 days in Red Brown, Yellow Brown, Gray Brown, Black and White strains, respectively and the CVs were between 9.33 and 10.11%. The egg weights at the first egg and 270 days of age were 33.2 and 50.8, 32.2 and 49.2, 32.2 and 49.1, 33.0 and 50.0, 30.7 and 47.8g respectively. The CVs of the egg weights of the first egg and at 270 days of age were 13.54~15.27% and 6.93~7.36%. The numbers of eggs produced by 270 days of age were 77.0, 79.3, 77.3, 73,7, 75.4, respectively, and observed CVs were between 29.98~36.99%. The significant strain effects were investigated in all the major economic traits in Korean native chicken. The significant strain effects were investigated in all the major economic traits in Korean native chicken. The highest least square (LS) means of the body weights at 150 and 270 days of age were observed in Black strain as 1,594.38 and 1,911.57g. The earliest LS mean of the ages at first egg was 146.88days in White strain. The heaviest LS means of egg weights at the first egg and 270 days of age were observed in Red Brown strain as 32.20 and 50.74g. The LS mean of the largest number of egg production was 79.50 eggs in Yellow Brown strain. Also, The significant generation effects were investigated in all the major economic traits. The highest LS means of the body weights at 150 and 270 days of age were observed in the generation 3 as 1,599.74 and 1,905.01g. The earliest LS mean of the age at first egg was 143.31 days in 4th generation. The heaviest LS means of egg weights at the first egg and 270 days of age were observed in 7th and 5th generation as 35.68 and 50.42g. The LS means of the largest number of egg production was 78.53 eggs in generation 6. In general, light body weight, short time for the age at first egg, heavy egg weight, and large number of egg production were observed as the generation proceeded.

• Korean Journal of Poultry Science
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• v.38 no.2
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• pp.137-144
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• 2011

The current work was carried out to investigate the effect of crossbred Korean native chickens (KNC) on fatty acid, amino acid and nucleotide-related compounds contents of chicken meat. A total of 360 male chicks (1d of age) was used in this work and were divided 4 groups as A) (KNC egg-meat type C strains ${\times}$ KNC meat type S strains) ${\times}$ Ross broiler, B) (KNC egg-meat type C strains ${\times}$ KNC meat type H strains) ${\times}$ KNC meat type S strains, C) (KNC native R strains ${\times}$ KNC meat type S strains) ${\times}$ KNC meat type H strains and D(White Semibroiler Chickens) strains for 5 weeks at the flat house. Palmitic acid and vaccenic acid were highest in C strain meat, and myristic acid and linolenic acid were lowest in A strain meat (p<0.05). Saturated fatty acid was lowest in C strain meats (p<0.05). Valine, leucine, phenylalanine and lysine of essential acid were low in A strain meat. Cystine, aspartic acid, glycine, alanine and proline were also low in A strain meat. Hypoxanthin (Hx) was high compared other strains at 5 weeks and low at 10 weeks. IMP was high compared other strains at 5 and 10 weeks. AMP has not significant difference among strains at 5 weeks but B strain was high other strains at 10 weeks. These results showed that C stain was excellent on the fact of nutrients compared to other strains. Consequently, the result of this work gave the basic data that needed to develope the new strains.

• Journal of Applied Biological Chemistry
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• v.44 no.4
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• pp.167-172
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• 2001

A cDNA fragment encoding the chloroplastic fructose-1, 6-bisphosphatase (FBPase) was cloned via PCR from the cDNA library of pea leaves. The cloned cDNA, about 1.05 kbp without signal sequence, was introduced into a pET-28a vector for expression in E. coli strain BL21(DE3)pLysS. The recombinant FBPase was purified through $Ni^+-NTA$ affinity chromatography and characterized. Molecular mass of the monomer was about 42,000. Enzymatic activity of the purified enzyme as the native pea chloroplastic FBPase was the highest at alkaline pH (pH 9.0). The recombinant enzyme was activated by a reducing agent DTT and was insensitive to AMP. The activation energy (Ea) and Arrehenius frequency factor were 42.67 kcal/mol and $2.65{\times}10^{14}/s$, respectively, slightly higher than those of the native enzyme. $K_M$ and $V_{max}$ were $99.98{\mu}M$ and $52.9{\mu}M/min$, respectively, which were comparable with the native enzyme.

• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.91-97
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• 2012

The strategy for infectious bursal disease (IBD) control and its success rate under field conditions depends on hygiene management, IBD field pressure, level, and variation in maternally derived IBD antibodies. This study investigated the level of IBD-specific antibody by ELISA and the prevalence of IBD virus by PCR in broilers, white-semi broilers, and Korean native chickens raised in Jeongeup, Jeonbuk. IBD-specific maternally derived antibodies were measured from 698 chickens and the mean titers of maternal antibodies were $3,572{\pm}1,402$ in broilers, $1,262{\pm}762$ in white-semi broilers, and $1,932{\pm}912$ in Korean native chickens. At 2 weeks after vaccination, the geometric mean antibody titers of broiler, white-semi broiler, and Korean native chicken were $582{\pm}427$, $3255{\pm}1,080$, and $1,023{\pm}499$, respectively. According to sequence analysis of the variable virion protein 2 gene, 4 isolates were found to be very virulent IBDV, 9 isolates classical virulent, and 2 isolates intermediate plus vaccine strain.

• Journal of Environmental Health Sciences
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• v.12 no.2
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• pp.67-74
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• 1986

The study was carried out to investigate for the property of Bacillus strains, on the native growthed microflora in Korean native soybean paste, and Bacillus strains of the high enzyme producing, were selected and identificated, from the microflora, that is, identificated Bacillus strains beared resemblance to B. subtills, on the colony, appearance was pellicle, surface's spreading, color creamy-thin browned, colony elevation flated, and edge lobated, the identfficated B. subtills strain named for the B. subtilis SCF. For the protease activity of B. subtilis SCF, according to the variation with pH, the pH stability was pH 7~8, and on the its protease activity, optimum temperature was 40$\circ$C, on the other hand, temperature of the highest stability of the protease was 50$\circ$C.