Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Production of Cellulosic Ethanol in Saccharomyces cerevisiae Heterologous Expressing Clostridium thermocellum Endoglucanase and Saccharomycopsis fibuligera β-glucosidase Genes

  • Jeon, Eugene (School of Life Science and Biotechnology, Korea University) ;
  • Hyeon, Jeong-eun (School of Life Science and Biotechnology, Korea University) ;
  • Suh, Dong Jin (Clean Energy Research Center, Korea Institute of Science and Technology) ;
  • Suh, Young-Woong (Clean Energy Research Center, Korea Institute of Science and Technology) ;
  • Kim, Seoung Wook (Department of Chemical Biological Engineering, Korea University) ;
  • Song, Kwang Ho (Department of Chemical Biological Engineering, Korea University) ;
  • Han, Sung Ok (School of Life Science and Biotechnology, Korea University)
  • Received : 2009.06.15
  • Accepted : 2009.08.17
  • Published : 2009.10.31
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Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and ${\beta}$-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an ${\alpha}$-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and ${\beta}$-glucosidase was able to produce ethanol from ${\beta}$-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Keywords

Acknowledgement

Supported by : Korea Science and Tngineering Foundation, National Research Foundation of Korea

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