• Title/Summary/Keyword: Native Plant

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A Unique Strategy for Recovering Recombinant Proteins from Molecular Farming: Affinity Couture on Engineered Oilbodies

  • Seon, Jeong-Hoon;J.Steven Szarka;Maurice M. Moloney
    • Journal of Plant Biotechnology
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    • v.4 no.3
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    • pp.95-101
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    • 2002
  • Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

Biosynthesis of Plant-Specific Flavones and Flavonols in Streptomyces venezuelae

  • Park, Sung-Ryeol;Paik, Ji-Hye;Ahn, Mi-Sun;Park, Je-Won;Yoon, Yeo-Joon
    • Journal of Microbiology and Biotechnology
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    • v.20 no.9
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    • pp.1295-1299
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    • 2010
  • Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone $3{\beta}$-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.

Chromosome number report of three Carex sect. Mitratae taxa (Cyperaceae) in Korea

  • CHUNG, Kyong-Sook;IM, Hyoung-Tak
    • Korean Journal of Plant Taxonomy
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    • v.50 no.3
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    • pp.361-367
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    • 2020
  • We report meiotic chromosome numbers of three taxa in Carex sect. Mitratae in Korea: Carex breviculmis R. Br. (n = 32II, 33II, 34II), C. polyschoena H. Lév. & Vaniot (n = 37II, 38II), and C. sabynensis Less. ex Kunth (n = 27II). Section Mitratae is one of the most species-rich Asian groups in Carex, comprising approximately 45-80 taxa. Twenty-seven of these occur in Korea, and they are some of the most challenging taxa to identify due to their obscure and inconspicuous diagnostic characters. Including the counts reported here, half of the native Korean sect. Mitratae chromosome numbers have been documented. Their haploid chromosome numbers range from n = 10 to n = 40, and many exhibit variations in the numbers counted within a taxon. These variations, along with the overall significant variation in sect. Mitratae, suggest that dynamic chromosome activity may be related to the high species diversity of Carex.

Selection of disease resistant lines of Rehmannia glutinosa with mutant treatment

  • Yu, Chang-Yeon;Lee, Youn-Su
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.4
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    • pp.273-276
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    • 2002
  • Rehmannia glutinosa is one of the most important medicinal crops in Korea. However, various plant pathogens including Fusarium sp. cause great damages and cause enormous economic losses. Therefore, this study was conducted to select Fusarium resistant plants by using mutagen-treated Rehmannia glutinosa. The plant material used was a native accession of Rehmannia glutinosa. As a result, among the Rehmannia glutinosa treated with various concentrations of EMS, R. glutinosa treated with 0. 03M EMS showed higher resistance against infection of F. oxysporum in pot tests, and Rehmannia glutinosa plants treated with 0.12M EMS showed higher resistance against infection of F. oxysporum in field tests.

Initiation of Germination Characters of Plant Seed by Light Quality (광에 의한 종자의 발아특성 발현과 그 분화 및 형성에 관하여)

  • 최관삼
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.175-190
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    • 1987
  • Germination characters of the lettuce seed that received photoperiodic pretreatments of low or high temperature from the flowering to harvest. MSU-15 seed, one of the lettuce cultivars used, having high dark germination, was modified to low dark germination by the long-day treatments during the seed formation. Light-requiring MSU-16 seed was modified to the dark-germination seed by high temperature given at the seed formation period. Above results suggest that the environmental conditions given to an immature seed adhered to a mother plant bring about some modification to its native germination habit. I confirmed that the spectral quality of light could influence the phytochrome system which controlled germination characters of the progeny of lettuce seed ; plants grown in light rich in far-red energies produced light-requiring seed, but those grown in high deficient in far-red energies produced dark germination seed.

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A Study on the Ecological Management Planning of Urban Forest (도시림의 생태적 관리계획에 관한 연구)

  • 이경재;류창희
    • Journal of the Korean Institute of Landscape Architecture
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    • v.20 no.4
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    • pp.1-11
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    • 1993
  • This study was conducted to investigate the plant community structure and to propose ecological management planning for the urban forest on Sungdongku, Joongku and Yongsanku in Seoul. Field survey was excuted October 1991 and the results were as follows. Robinia pseudoacasia community covered 61.1% and Populus x albaglandulosa covered 3.8% of survey site II and survey site was covered by the deciduous-broad leaf mixtured forest. According to the human impact and inappropriate management impact, survey area was divided into five forest community. As for the analysis of plant community structure which was classified into five forest community, the importance values, ecological diversity, number of species, number of individuals, basal area and crown coverage of the native plant community showed relatively higher than community that vegetation deterioration degree by the human impact showed seriously. In landscape forest of Mt. Daehyun, it showed problems for the planting method and the soil condition. So it was proposed to management guide, vegetation restoration and soil management through ecological management planning based on above results.

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Etymological Study of Species in Scientific Names of Landscape Plants. (조경식물의 학명에서 종명의 어원 연구)

  • 최상범
    • Journal of the Korean Institute of Landscape Architecture
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    • v.21 no.2
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    • pp.8-16
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    • 1993
  • The purpose of this paper is to analyze the linguistic origin of the species manes of landscape plants in order to help students understand the physical characteristics of the plants through the botanical names. This study includes the plants that are possible to use as landscape material as well as both the native and foreign plants that are already used. Name of Species of Scientific name in Landscape plant be derived from region or nations of habitat, botanist, plant explors, and their sponser, and plant form and number of leaves or flowers, character or ingredient of plants and shape of stem, tree crown form. In this paper, about 300 species(contains varieties, formas) are interpreted into etymological meanings.

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Bremia itoana (Oomycota, Peronosporales), a Specialized Downy Mildew Pathogen on an East Asian Plant, Crepidiastrum sonchifolium (Asteraceae)

  • Choi, Young-Joon;Park, Ji Hoon;Lee, Jeongran;Shin, Hyeon-Dong
    • Mycobiology
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    • v.46 no.4
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    • pp.416-420
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    • 2018
  • Crepidiastrum sonchifolium, a flowering plant in the daisy family (Asteraceae), is native to East Asia. In Korea, this plant is a locally cultivated vegetable, and its market size is gradually growing. Since the plants with downy mildew infection were initially found at a private farm of Chuncheon city, the occurrences have continued in commercial farms of other regions, highlighting that this disease is spreading throughout Korea. The pathogen was attributed to a member of the genus Bremia that contains many specialized species, each of which displays a narrow host spectrum on Asteraceae. Based on morphological and molecular phylogenetic analyses, along with the high host specificity recently proven for Bremia species, the identity of the causal agent was confirmed as a so far undescribed species of Bremia. Here, we introduce Bremia itoana sp. nov., specific to C. sonchifolium.

The complete chloroplast genome sequence of Hibiscus sabdariffa (Malvaceae)

  • KWON, Soon-Ho;PARK, Yunmi;JANG, You Lim;KWON, Hae-Yun
    • Korean Journal of Plant Taxonomy
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    • v.52 no.2
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    • pp.123-126
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    • 2022
  • Hibiscus sabdariffa L., (roselle) in the Malvaceae family is an erect subshrub known to be native to India and Malaysia. It is widely used as a food or tea material around the world, and its therapeutic effects have been widely studied. In this study, the sequencing of the complete chloroplast genome of H. sabdariffa was carried out. The result indicates a genome size of 162,428 bp, which is composed of a large single copy of 90,327 bp, two inverted repeats of 26,242 bp each, and a small single copy of 19,617 bp. Overall, a total of 131 genes were predicted, including 86 coding sequences, 37 tRNAs, and 8 rRNAs. According to a phylogenic analysis, it was clearly distinguished from outgroups such as other species of the genus Hibiscus used in the analysis.

A newly recorded alien plant, Silene fissipetala(Caryophyllaceae) from Korea

  • Jung-Hyun Kim;Yang-Hoon Cho;Seok-Soon Kim;Sunhee Sim;Min-Ha Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.266-272
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    • 2023
  • Silene fissipetala Turcz., which is native to Continental China and Taiwan, was newly found in Chungcheongnam-do, Korea. S. fissipetala is similar to the related taxa S. antirrhina L., S. armeria L., and S. koreana Kom. in that it has glutinous zones. However, S. fissipetala is distinguished from the congeneric species by the presence of laciniate at the petals. The species grows on slopes and roadsides, suggesting that it is likely to have been introduced through the installation of green sites and road construction. A precise description, photographs, voucher specimens and a key to related taxa are provided.