• Title/Summary/Keyword: National development

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Determination of the effective spray- interval of Bacillus thuringiensis against diamond-back moth (Plutella xylostella) on chinese cabbage

  • Han, E.J.;Park, J.H.;Hong, S.J.;Ahn, N.H.;Jee, H.J.;Kim, Y.K.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.259-262
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    • 2011

  • In organic Chinese cabbage fields, Commercial Bacillus thuringiensis products are used widely against diamond back moth, Plutella xylostella. We conducted the study to determine the effective spray-interval of commercialized B. thuringiensis against diamond back moth on Chinese cabbages. Chinese cabbage leaves were collected 0, 1, 2, 3, 6, 10days after treatment in first trial and 0, 2, 4, 7, 9, 11days after treatment. We compared the insecticidal property of sprayed B. thuringiensis and the density of it on surface of Chinese cabbages using collected leaves. The insecticidal property maintained high until nine days after commercial B. thuringiensis products sprayed.

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Complete Genome Sequence of a Staphylococcus aureus Sequence Type 188 Strain SA73-2 Isolated from Lettuce

  • Kwang-Kyo Oh;Seung-Mi Seo;Hyunsuk Jin;Bo-Eun Kim;Husna;Dong Suk Park
    • Microbiology and Biotechnology Letters
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    • v.51 no.4
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    • pp.559-561
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    • 2023

  • Staphylococcus aureus is a ubiquitous pathogen associated with both human and animal diseases. In this study, we present the complete genome sequence of SA73-2, which was isolated from lettuce at an agricultural farm in South Korea. The assembled genome consists of a 2,750,304-bp circular chromosome and a 24,654-bp plasmid. This strain belongs to sequence type 188 (ST188), with spa type 189, and carries the β-lactamase gene (blaZ) on the plasmid.

  • https://doi.org/10.48022/mbl.2310.10018 인용 PDF

Anti-Inflammatory Activity of Antimicrobial Peptide Periplanetasin-5 Derived from the Cockroach Periplaneta americana

  • Kim, In-Woo;Lee, Joon Ha;Seo, Minchul;Lee, Hwa Jeong;Baek, Minhee;Kim, Mi-Ae;Shin, Yong Pyo;Kim, Sung Hyun;Kim, Iksoo;Hwang, Jae Sam
    • Journal of Microbiology and Biotechnology
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    • v.30 no.9
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    • pp.1282-1289
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    • 2020

  • Previously, we performed an in silico analysis of the Periplaneta americana transcriptome. Antimicrobial peptide candidates were selected using an in silico antimicrobial peptide prediction method. It was found that periplanetasin-5 had antimicrobial activity against yeast and gram-positive and gram-negative bacteria. In the present study, we demonstrated the anti-inflammatory activities of periplanetasin-5 in mouse macrophage Raw264.7 cells. No cytotoxicity was observed at 60 ㎍/ml periplanetasin-5, and treatment decreased nitric oxide production in Raw264.7 cells exposed to lipopolysaccharide (LPS). In addition, quantitative RT-PCR and enzyme-linked immunosorbent assay revealed that periplanetasin-5 reduced cytokine (tumor necrosis factor-α, interleukin-6) expression levels in the Raw264.7 cells. Periplanetasin-5 controlled inflammation by inhibiting phosphorylation of MAPKs, an inflammatory signaling element, and reducing the degradation of IκB. Through LAL assay, LPS toxicity was found to decrease in a periplanetasin-5 dose-dependent manner. Collectively, these data showed that periplanetasin-5 had anti-inflammatory activities, exemplified in LPS-exposed Raw264.7 cells. Thus, we have provided a potentially useful antibacterial peptide candidate with anti-inflammatory activities.

  • https://doi.org/10.4014/jmb.2004.04046 인용 PDF KSCI