• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.463-469
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• 2010

Rice is a staple food for more than 50% of the world's population. Embryo comprises only 2 to 3% of the weight distribution of the entire pericarp but has higher concentration of vitamins, proteins, and essential fatty acids than the other parts of grains. Moreover, ${\alpha}$-tocoperol, ${\gamma}$-oryzanol, phytic acid and ${\gamma}$-aminobutric acid that have nutraceutical value are abundant. Increasing the volume of embryo assures the fortification of nutritional value of rice grain. We developed new black waxy giant embryo rice, Milyang 263 by crossing Josaengheugchal, a black waxy rice variety, and $ge^t$, a giant embryo mutant generated by tissue culture. The nutrient contents and physical properties of Milyang 263 were compared with several giant embryo mutants and normal embryo rice varieties. Changes in the nutrient properties after germination were also observed. Results indicated that this new black waxy giant embryo rice, Milyang 263, offers a promising source for improving nutritional quality of rice especially anthocyanin, essential minerals, and GABA.

• Korean Journal of Veterinary Service
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• v.43 no.4
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• pp.245-250
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• 2020

Infectious bovine keratoconjunctivitis (IBK) is the most common ocular disease in cattle, manifesting as corneal opacity, corneal ulcerations and potentially vision loss. The present report describes a 10-month-old Holstein Friesian heifer with IBK treated by systemic tulathromycin, and subconjunctival injection of penicillin and dexamethasone. We investigated changes in the hematological indices and microorganisms related to IBK after treatment. Neutrophils and monocytes decreased during recovery, so it was assumed that these two types of white cells are associated with IBK. Moraxella bovoculi was cleared in the eye, nasal cavity, and oral cavity after treatment. The distribution of M. bovoculi before treatment indicated that a combined systemic and subconjunctival treatment was necessary. The lesioned eye was found to be overwhelmed by Mycoplasma bovoculi, while pathogen abundance was reduced in the nasal cavity and oral cavities. These results suggest that antibiotic treatment can alter the composition and relative abundance of microorganisms.

• Plant Breeding and Biotechnology
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• v.2 no.3
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• pp.289-300
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• 2014

A tissue-specific and developmentally expressed gene was isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis), designated BrREF (B. rapa Rubber elongation factor). BrREF transcripts were expressed at high levels in seedlings and at low levels in flower buds and roots. To study the activity of this promoter, the 2.2 kb upstream sequence of BrREF gene was fused to a β-glucuronidase (GUS) reporter gene and was introduced into Arabidopsis thaliana and B. napus by Agrobacterium-mediated transformation. Strong expression of GUS driven by the BrREF promoter was detected in the cotyledons and hypocotyls of transgenic plant seedlings, but GUS expression was weak in roots, excluding the root tips. GUS expression in the cotyledons and hypocotyls decreased dramatically as the seedlings matured and was not detected in the tissues of mature plants. During floral development, GUS expression was observed in immature anthers. These findings suggest that the BrREF promoter can modulate the tissue-specific and developmental expression of gene at the early stages of growth and development.

• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.227-228
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• 2009

• Natural Product Sciences
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• v.23 no.3
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• pp.217-221
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• 2017

Eleven compounds (1-11) were isolated from the rhizomes of Osmunda japonica, and their structures were elucidated based on $^1H$, $^{13}C-NMR$ and LC-IT-TOF MS data. Of these compounds, all compounds (1 - 11) have been previously reported, although five (6 - 9, 11) have not previously been isolated from this plant. The antioxidant activities of isolated compounds (1 - 11) were measured by DPPH and ABTS assays, and compound 10 showed the high antioxidant activity.

• Korean Journal of Veterinary Service
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• v.41 no.1
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• pp.47-49
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• 2018

A 2-month-old female Holstein Friesian calf was noted to leak urine from the umbilicus during urination. She urinated through both the umbilicus and the urethra. A conduit from the umbilicus to the bladder was identified with ultrasonography. Uroperitoneum was ruled out because blood urea nitrogen and creatinine were within reference ranges. Surgery was performed to resect the patent urachus. Urachitis was identified during surgery. Dexamethasone and an antibiotic were used for the inflammation and postoperative cares. Meloxicam was substituted for dexamethasone since the calf showed side effects to dexamethasone. The calf recovered completely and there was no urine leakage from the umbilicus.

• Food Science of Animal Resources
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• v.36 no.4
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• pp.458-462
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• 2016

The effect of ultra-sonication on quality characteristics and flavor of baked eggs was studied. One hundred and twenty eggs were cooked and assigned to six treatments groups (n=20 each) that were then soaked in saline solution at various concentrations (5, 10 and 18%) with/or without further ultra-sonication treatment at 35 kHz for 1 h. The pH values were lower in the ultra-sonicated samples in comparison with the non-ultra-sonicated samples (p<0.05). The values for texture traits were higher in the samples soaked in 10% saline solution with ultra-sonication in comparison with other remaining treatments or control (p<0.05). The sodium content in samples soaked in 10% saline solution with ultra-sonication was similar to that of the ones soaked in 18% saline solution without ultra-sonication. The higher flavor scores were also given for the ultra-sonicated samples in comparison with the control or non-ultra-sonicated ones. These results suggest that the application of ultra-sonication may produce a faster sodium penetration into baked eggs, simultaneously improves some textural traits (e.g., hardness) as well as flavor of the products.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.1
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• pp.35-44
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• 2021

A pregnancy diagnosis is an important standard for control of livestock's reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows ("open" cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy ("open" cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.

• Research in Plant Disease
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• v.22 no.2
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• pp.111-115
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• 2016

Wilt disease appeared the first in greenhouse-grown safflower (Carthamus tinctorius) in Jeonju, Korea. With the advancement of the disease, the infected plants were withered and died. In order to investigate the causal organism of this symptom disease, fungus was isolated from the infected plants and cultured on potato dextrose agar medium. The fungus showed the white or orange colony color with aerial mycelium. Macroconidia were from falcate to straight, usually 3-5 septate with $38.0-66.7{\times}2.9-4.4{\mu}m$. The fungus was inoculated to a new safflower plant and caused the same wilt. With morphological characters and pathogenicity results, sequence analyses (internal transcribed spacer ribosomal DNA and translation elongation factor $1{\alpha}$) suggested that, the isolated fungus is Fusarium proliferatum. This is the first report of Fusarium wilt disease caused by F. proliferatum on safflower in Korea.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.51-51
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• 2020

콩의 원산지는 만주를 중심으로한 동북아시아를 원산지로 추정하고 있으며, 동아시아인 한국, 중국, 일본의 주요 식재료로 다양하게 이용되어왔다. 콩은 단백질과 지방의 공급원으로서 뿐 아니라 최근에는 콩의 생리활성 물질이 항암효과, 골다공증, 신장질환과 같은 질병예방에도 효과가 있는 것으로 알려지면서 활용가능성이 높아지고 있다. 본 연구는 유전자원센터에 보존 중인 콩 수집단에서 농업형질, 기초정보 등을 토대로 핵심집단을 작성하고 그 중 한국, 중국, 일본 수집단 각 425, 93, 42자원의 개화일수, 생육일수, 주당협수, 100립중, 주당수량과 같은 양적형질을 비교하였다. 개화일수와 생육일수는 중국수집 자원에서 평균38일, 107일로 가장 빠르며, 한국수집 자원은 평균 53일, 127일로 가장 늦었다. 협당립수는 중국수집 자원에서 협당 평균3립으로 가장 많았으며, 한국과 일본 수집자원은 각 2.3과 2.4립으로 유사하였다. 주당협수는 한국 수집자원에서 주당 평균 327.4개로 가장 많고 일본이 248.0개 중국이 224.4순으로 조사되었다. 100립중은 일본 수집 자원이 평균 33.6g으로 대립이 많고 한국 26.5g , 중국 21.1g 이었다. 주당수량은 한국 수집자원이 평균 119.8g, 일본 84.2g, 중국 76.8g순이었다. 이들 5개 양적형질을 토대로 군집분석한 결과 한국 자원은 대다수가 C3 그룹에 속하고, C1 그룹에 일부가 속하였으며, 일본 자원은 C3 그룹과 C2 그룹에, 중국 자원은 C2 그룹에 속하여 한국과 일본자원이 유사하고 중국자원과는 차이를 보이는 것으로 나타났다. 이러한 특성조사 결과는 자원의 수집, 도입시 다양성 확보를 위한 기초정보로 이용할 수 있다.