• 생명과학회지
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• 제33권1호
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• pp.64-72
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• 2023

심장 발생과정에 관여하는 주요 전사인자들의 기능에 대한 규명 등의 발전에도 불구하고 줄기 세포에서 매우 효율적인 심근 세포로의 분화를 촉진하는 새로운 생체 활성 분자를 찾는 것이 여전히 필요하다. 마우스배아줄기세포(mESC) 유래 심근세포의 Illumina 발현 마이크로어레이 데이터를 분석하였다. 미분화 mESCs와 비교하여 mESC 유래 심근세포에서 4배 이상 유전자 발현이 증가한 276개 유전자가 스크리닝되었다. Secreted phosphoprotein 2 (Spp2)는 후보물질 중 하나이며 bone morphogenetic protein 2 (BMP2)에 대한 슈도수용체로서 BMP2 신호 전달을 억제하는 것으로 알려져 있다. 그러나 심근 형성과의 연관성은 알려진 바 없다. 우리는 mESC 세포주인 TC-1/Kh2와 E14를 이용하여 기능성 심근세포로 분화하는 동안 Spp2 발현이 증가함을 검증하였다. 흥미롭게도, Spp2 분비는 배아체(embryoid body, EBs) 형성 후 3일차에 일시적으로 증가했는데, 이는 Spp2의 분비가 ESCs의 심근세포로의 분화에 관여함을 시사한다. Spp2의 기능을 분석하기 위해, 우리는 BMP2를 처리하면 분화 경로를 근모세포에서 골모세포로 전환되는 특성을 가진 C2C12 마우스 근모세포 세포주를 사용하여 실험을 수행하였다. mESCs의 분화와 유사하게, Spp2의 전사는 C2C12 근모세포가 근관으로 분화됨에 따라 증가하였다. 특히, 분화 초기 단계에서 Spp2의 세포외 분비가 극적으로 증가하였다. 또한, Spp2-Flag 재조합 단백질로 처리하면 C2C12 근모세포의 근관으로의 분화가 촉진되었다. 종합하면, ESCs를 심근 세포로 분화시키는 새로운 생체 활성 단백질로 Spp2를 제안한다. 이것은 심근형성의 분자 경로를 이해하고 허혈성 심장질환에 대한 줄기세포 요법의 실험적 또는 임상적 발전을 촉진하는 역할을 할 것으로 기대한다.

• 수산해양기술연구
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• 제52권3호
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• pp.197-208
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• 2016

The WCPFC CMMs related to tropical tunas have been strongly restricting fishing capacity and operating activities of main fisheries, especially purse seine fishery. In terms of changes in fishing trend by implementing relevant measures of FAD set, the number of FAD set by Korean purse seine fleet showed a decreasing pattern with the lowest level of FAD set proportion among major distant water fishing nations. With regards to the FAD closure, there was no significant change in fishing effort but a drop in catch, especially bigeye tuna during the period of FAD closure. Moreover, it showed closely similar trends between the number of FAD set and bigeye tuna catch. In terms of measures related to the high sea area including a ban on fishing on the HSP and limitation of fishing days on the high sea by flag state, proportions of catch and effort on the high sea had sharply declined after implementing those measures. As relevant measures are expected to be strengthened, it should pay attention to change of coastal nations policies and focus on improving fishing efficiency of unassociated school set with multi-pronged efforts.

• 복식
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• 제61권10호
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• pp.42-54
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• 2011

The study was focused on surveying the cultural identity and the origin of T-shirts, and examining their functions. Six cities were selected to categorize the design motifs of cultural identity appearing in the design of contemporary T-shirts. These cities include a city in France, United Kingdom, Italy, United States and Japan where collections are currently presented at, and also a city in China that has shown a high economic growth as one of the most popular sightseeing cities. Cultural identity was presented in the T-shirts that were examined in this study through diversified design motifs. Motifs used in T-shirt designs to reflect cultural identity included national flags, notable places, letters, names of geographic places, and individuals. A national flag is a motif that signifies national identity and it was used in the front of T-shirts as it is or was just partially used. Also, many world-renowned structures or architectural buildings were applied to T-shirt design. Noted places such as the White House, the Statue of Liberty in the U.S., Tienanmen Square in China and the Eiffel Tower in France were used. The unique characters of language, such as Hangeul in Korea were used as design elements to reflect the structural characteristics of letters. Names of geographic places were used as design elements through the disclosure of the name of a symbolic city or a tourist site in each country. In terms of figures, not only the historical figures or celebrities from each country were used, but the image of bodies and faces that represent each culture were used as well. In addition, this study was used to develop T-shirt designs that reveal the cultural identity of Korea.

• 한국초지조사료학회지
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• 제43권3호
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• pp.162-167
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• 2023

The experiment aim to breed an early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.). It was conducted at the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea, from 2022 to 2023. The new variety named "Oasis" is a diploid with green leaves and has a semi-erect growth habit in late fall, and an erect growth habit in early spring. With a heading date of May 10, Oasis is categorized as an early-maturing variety. Compared with the "Florida80" as a control variety, Oasis's flag leaf was 1 mm wider and 2 cm shorter, while the plant length was 7.8 cm longer. Additionally, the ear of Oasis was 1.2 cm longer than that of Florida80, and it showed lodging resistance. The dry matter yield of Oasis (8,805 kg/ha) was higher than that of Florida80. The total digestible nutrient and crude protein contents of Oasis were 64.5%, and 9.3%, respectively, which were 1.1% and 0.6% higher than those of Florida80, respectively. The neutral and acid detergent fiber contents in Oasis were 54.2% and 30.8%, respectively, which was 1.9% and 1.4% lower than those of Florida80, respectively. These results indicate that Oasis has good dry matter yield in the most of region and especially, middle and northern region of South Korea.

• 한국수산과학회지
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• 제57권4호
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• pp.467-478
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• 2024

The fish assemblage characteristics in the coastal waters of Sinchang-ri in the Western region of Jeju Island were determined using trammel net samples collected from 2012 to 2022. During the survey period, 81 fish species (43 families, 12 orders) were identified. Tetraodontiformes (4 families, 9 species) and Perciformes (26 families, 57 species) accounted for 84% of the total number. Annual species variation was highest in 2017 and 2019 (36 species) and lowest in 2015 (26 species). Seasonal species variation was higher in summer (peak, 58 species) than winter (peak, 41 species). The number of individuals (1,509) and biomass (446.5 kg) peaked in August (summer) and were lowest in February (winter; 415 individuals, and 150.7 kg). 41 (50.6%) subtropical fish species were identified, and the number of species was highest in 2020 {21 (61.8%) and lowest in 2014, 2017 [16-18 (47.1-47.4%)]}. Subtropical fish species, individuals and biomass were significantly correlated with environmental factors (summer temperatures) at the sampling sites. Scarbreast tuskfish Choerodon azurio, balloon fish Diodon holocanthus, flag fish Goniistius zonatus, bluestriped angelfish Chaetodontoplus septentrionalis, hairy stingfish Scorpaenopsis cirrhosa, threeline grunt Parapristipoma trilineatum, and blackfin sweeper Pempheris japonica were the dominant species of subtropical fish in the study sites.

• Fisheries and Aquatic Sciences
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• 제14권1호
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• pp.62-69
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• 2011

The effects of actively stimulating devices (ASD) on juvenile flatfish escape were studied to increase escape rates from the cod end by encouraging fish to approach the net wall. Two kinds of ASD were designed: a fluttering net panel, i.e., a free-end flag-like net panel, and a double conical rope array. Escape responses of juvenile bastard halibut were observed in a circulating water channel using two model cod ends, one made with diamond-shaped 43-mm-mesh-size polyethylene (PE) as a high-contrast cod end and the other with polyamid (PA) mono-ply as a low-contrast cod end. Retention rate was significantly lower with the double conical rope-array ASD in the PE cod end than with conventional PE cod ends only or the fluttering net-panel ASD inside the PE cod end. Mean retention rate with the low-contrast PA cod end was also significantly lower than that with the high-contrast PE conventional cod end. Therefore, active fluttering devices using a double conical rope array together with less visible low-contrast netting in the cod end could help to reduce the bycatch of juvenile flatfish by weakening their optomotor response and actively driving fish to the side net panel.

• 한국항만경제학회지
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• 제36권1호
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• pp.23-40
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• 2020

본 연구는 IMO 2020 시행에 따른 국내외 선사의 대응전략을 살펴보고, 선사의 주요 대응방안인 저유황유로 연료를 전환할 경우 국적선사가 부담해야 할 추가비용과 그것이 선사의 수익성에 미치는 영향을 시나리오별로 분석하였다. 분석결과, 저유황유 사용 시 국적선사는 상당한 추가 비용이 발생하게 되고 이로 인해 모든 시나리오에서 영업이익 적자를 기록하는 등 수익성이 악화될 것으로 분석되었다. 이러한 결과는 대화주 운임교섭력이 약한 근해선사의 경우 연료유 가격에 탄력적으로 적용할 수 있는 합리적인 BAF 설정 기준이 필요하며, 또한 정유사, 화주, 선사 간 상호협력을 통해 상생할 수 있는 방안을 모색할 필요가 있음을 시사한다.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.233-236
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• 2006

Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

• The Plant Pathology Journal
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• 제16권3호
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• pp.147-150
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• 2000

Sporulation patterns of rice blast fungus were studied at relatively later stages of leaf blast and neck blast seasons in Icheon, Korea. This experiment was done by detaching lesion-bearing leaves and panicle bases. The number of conidia remaining on the leaf blast lesions of different cultivars from Jul 20 to Jul 23 ranged from 3,640 to 82,740 spores. More conidia were observed on the adaxial surface because they were released from abaxial surface. After heading, sporulation was observed from the lesions on the flag leaves but the number of spores was less than in the late July. Detached panicle bases or uppermost internodes infected by Pyricularia grisea produced abundant amount of conidia. Among these panicle bases, 30.1 mm size lesion recorded the highest count of 244,560 spores. When we compared the sporulation amount using the KY-type spore trap, more conidia were recorded from intact lesions than from the lesions which removed conidia and conidiophore The ratio of conidia release against total sporulation ranged from 20.5%-25.0% for leaf blast and 8.2%-25.3% in the neck blast. Effective inoculum potential was also discussed.

• 한국작물학회지
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• 제43권3호
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• pp.161-167
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• 1998

Root and shoot development of two rice (Oryza sativa L.) cultivars with different genetic backgrounds was studied with reference to their relative growth. Tongil type (indica-japonica hybrid) cultivar 'Kuemkangbyeo' and japonica cultivar 'Koshihikari' were grown in $5000^{-1}$ a Wagnar pots under flooded condition. Three plants with roots of both cultivars were taken in every phyllochron through the heading stage to record morphological characteristics of shoot and root system. Compared to Koshihikari, Kuemkangbyeo produced more tillers and had greater shoot weight and leaf area per hill. Length and weight of the root system in both cultivars increased exponentially with time. At the same time, root system development was significantly faster in Kuemkangbyeo than in Koshihikari after the panicle initiation stage. As a result, Kuemkangbyeo has a vigorous root system which consists of larger number of nodal roots compared to Koshihikari. Also, the root length and weight per unit leaf area of Kuemkangbyeo were larger than those of Koshihikari in the later half of growing period, which suggests possible higher physiological activity of the root system of Kuemkangbyeo which is known as a high-yielding cultivar. The relationship between root traits (crown root number, total root length, and root dry weight) and shoot traits (leaf area and leaf+culm dry weight) in both cultivars closely showed allometry until the flag leaf stage.