• 제목/요약/키워드: National Characters

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박테리오파지 저항성을 갖는 Pseudomonas tolaasii 변이주 분리 및 이들의 병원특성 (Isolation of bacteriophage-resistant Pseudomonas tolaasii strains and their pathogenic characters)

  • 박수진;한지혜;김영기
    • Journal of Applied Biological Chemistry
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    • 제59권4호
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    • pp.351-356
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    • 2016
  • 세균성 갈반병은 느타리버섯(Pleurotus ostreatus)의 주된 병중의 하나이다. 박테리오파지를 이용한 파지테라피 방법은 병원균의 농도를 감소시켜 병없는 재배사를 만드는데 성공적이었다. 병원균 사멸을 위한 파지의 이용은 숙주균의 특이성 때문에 매우 제한적 효과를 보이며, 이것은 병원균의 작은 변이에도 파지의 민감성은 크게 감소할 수 있기 때문이다. 본 연구에서는 파지테라피의 효용성을 높이기 위하여 갈반병의 원인균인 P. tolaasii 균주로부터 파지-저항성 변이주를 분리하였고 병원성 특성을 조사하였다. 16S rRNA 유전자의 염기서열 분석을 통한 근연관계 분석에서 파지저항성 균주들은 모두 원래의 숙주균과 일치하였고, 용혈활성이나 갈반형성 능력 등 병원성은 파지저항성 획득과 관련이 없는 것으로 확인되었다. 그럼에도 불구하고, 파지저항성 균주의 다양한 병원성은 균의 종류에 따라 증감이 다르게 나타났다. 따라서, 성공적인 파지테라피를 위해서는 넓은 숙주 범위를 갖는 파지의 분리가 필요하다.

덩굴딸기(Rubus oldhami)의 종내변이 (Intraspecific Variations of Rubus oldhami)

  • 김미나;장수길;유기억
    • 한국자원식물학회지
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    • 제18권2호
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    • pp.260-269
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    • 2005
  • 덩굴딸기의 종내 변이를 알아보기 위하여 꽃의 형질에 의해 유형화 한 3가지 유형에 대하여 외부형태학적 형질 분석, 주성분분석. 화분학적형질 분석, RAPD 및 PCR-RFLP분석을 실시하였다. 외부형태에서는 꽃잎과 꽃받침의 크기. 정소엽과 소엽의 크기 등이 3개의 유형을 구별하는데 유용한 형질이었지만 줄기, 화경의 길이, 잎의 거치형태, 엽축의 길이, 잎에 털의 분포양상 등은 변이가 없었다. 14가지의 외부형태형질을 이용한 주성분분석에서 3가지 변이형태는 유형별로 구별이 가능하였다. 화분학적 형질에서는 화분입상, 발아구의 수, 표면무늬 등에 있어 매우 유사한 형태로 나타나 구별이 불가능하였다. RAPD분석에서는 20개의 primer 중 8개가 분류군 전체에서 반응을 보였고 이로부터 총 33개($60\%$)의 다형화 밴드를 얻었으며, 유집분석 결과 type 2는 독립적으로 소그룹을 형성하였지만 다른 두 가지 변이형의 개체들은 서로 중복되어 유집되어 나타났다. PCR-RFLP분석은 엽록체 DNA 중 증폭된 일부 구간을 얻을 수 있었지만 제한효소 처리 결과에서는 다형화 밴드를 전혀 찾을 수 없었다. 이상의 결과에서 덩굴딸기의 종내변이 형태는 외부형태형질에 따라 유형별 구분이 가능하였지만 화분학적 형질이나 DNA수준에서는 전혀 뒷받침되지 못하였다. 따라서 type 1과 2는 정상개체와는 꽃의 형질에 차이가 있으나 동일한 자생지에 함께 분포하므로 자연잡종에 의한 일시적인 변이형이거나 생태종으로 보는 것이 타당할 것으로 생각된다.

Analysis of Isolation, Connection and Circulation with the Neighborhood park in Daegu

  • Ryu, Yeon-Su;Ra, Jung-Hwa;Cho, Hyun-Ju;Kim, Jin-Hyo;Kwon, Oh-Sung
    • Current Research on Agriculture and Life Sciences
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    • 제30권1호
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    • pp.13-25
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    • 2012
  • Major and reckless development which have been continued during the last half century, have caused decrease and damage of urban green spaces in the point of qualitative and quantitative view. Particularly, it brought about reduction to urban neighborhood park which has taken the main role for landscape ecological value. Recently, neighborhood park planning based on the landscape ecological results has been increasing gradually. Most of all, diverse attempts such as the application of shape character analysis and the step of landscape ecological planning in urban park planning have been proposed. Today, we recognize the importance of comprehensive approach in urban green planning and neighborhood park planning, but landscape ecological approach which is analyzing character and making proposal with isolation, connection and circulation is still insufficient. Most of neighborhood parks in Korea are surrounded by buildings and isolated from adjacent green spaces. Besides, these parks have landscape ecological problems such as reduction of size, isolation from adjacent green spaces, decline of nature, and excessive pavement which we ignored during urban development process. We have sympathy for understanding landscape ecological characters and considering improvement proposals for neighborhood parks Therefore, the purpose of this study was to 1) select five neighborhood parks in Daegu, 2) analyze landscape ecological characters with isolation, connection and circulation, and 3) compare data. It is certain that these results should be the main data for the arrangement of improvement proposals which landscape ecological characters were appled to.

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Triangulation Based Skeletonization and Trajectory Recovery for Handwritten Character Patterns

  • Phan, Dung;Na, In-Seop;Kim, Soo-Hyung;Lee, Guee-Sang;Yang, Hyung-Jeong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제9권1호
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    • pp.358-377
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    • 2015
  • In this paper, we propose a novel approach for trajectory recovery. Our system uses a triangulation procedure for skeletonization and graph theory to extract the trajectory. Skeletonization extracts the polyline skeleton according to the polygonal contours of the handwritten characters, and as a result, the junction becomes clear and the characters that are touching each other are separated. The approach for the trajectory recovery is based on graph theory to find the optimal path in the graph that has the best representation of the trajectory. An undirected graph model consisting of one or more strokes is constructed from a polyline skeleton. By using the polyline skeleton, our approach accelerates the process to search for an optimal path. In order to evaluate the performance, we built our own dataset, which includes testing and ground-truth. The dataset consist of thousands of handwritten characters and word images, which are extracted from five handwritten documents. To show the relative advantage of our skeletonization method, we first compare the results against those from Zhang-Suen, a state-of-the-art skeletonization method. For the trajectory recovery, we conduct a comparison using the Root Means Square Error (RMSE) and Dynamic Time Warping (DTW) in order to measure the error between the ground truth and the real output. The comparison reveals that our approach has better performance for both the skeletonization stage and the trajectory recovery stage. Moreover, the processing time comparison proves that our system is faster than the existing systems.

Fruit Yield and Quality Evaluation of Sweet Pepper (Capsicum annuum L.) F1 Hybrids Derived from Inbred Lines

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • 한국육종학회지
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    • 제42권4호
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    • pp.344-350
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    • 2010
  • Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.