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Herbicide Resistance in Plant Tissue Culture (식물체(植物體) 조직배양(組織培養)과 제초제(除草劑) 저항성(抵抗性))

  • Kim, Kil-Ung
    • Korean Journal of Weed Science
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    • v.5 no.1
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    • pp.9-13
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    • 1985
  • This study was conducted to evaluate herbicide resistant plant through tissue culture. Callus was induced from embryos of Echinochloa crusgalli Beauv. (var, oryzicola Ohwi, var. caudata Kitagawa and var, crusgalli). An optium medium for callus induction and succinate dehydrogenase activity in inducted callus were detected and callus growth of various varieties of Echinochloa crusgalli was assessed under the treatment of various rates of butachlor[N-(butoxymethyl)-2-chloro-N-(2,6-diethylphenyl)acetamide]. MS medium seemed to be the most appropriate to induce callus from the embryos of varieties of E. crusgalli by using 2,4-D about 5.5mg/l as a hormone source. The activity of succinate dehydrogenase in inducted callus showed positive reaction against to TTC(2,3,5-triphenyltetrazolium chloride) regardless of concentrations of butachlor and varieties of E. crusgalli, indicating that all the callus induced were alive. The callus growths derived from seeds of E, cnesgalli were greatly affected by various rates of butachlor and were completely inhibited at the highest concentration of butachlor, $10^{-3}M$, regardless of varieties of E. crasgalli. $10^{-6}M$ of butachlor inhibited 24.6% of the callus growth of E. crusgalli Beauv, var. oryzicola Ohwi, while E. crusgalli Beauv. var. crusgalli showed 42% of inhibition, showing that there was difference in response of varieties of E. crusgalli Beauv. to butachlor.

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Occurrence Patterns of Paddy Weeds and Distribution of Resistant Weeds to an ALS Inhibiting Herbicide in Jeonnam by a Soil Assay Method (토양검정법에 의한 전남지역 논잡초 발생양상과 ALS 저해제 제초제 저항성 논잡초 분포)

  • Jeong, Jang Yong;Yun, Young Beom;Jang, Se Ji;Hyun, Kyu Hwn;Shin, Dong Young;Lee, Jeongran;Kwon, Oh Do;Kuk, Yong In
    • Weed & Turfgrass Science
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    • v.7 no.3
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    • pp.191-199
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    • 2018
  • This study was to investigate the occurrence patterns of paddy weeds, their resistance levels to an ALS inhibiting herbicide, and to estimate the areas of resistance in these paddy fields. We used soil collected from 358 paddy fields of Jeonnam province in 2017. Based on their life cycles, weeds were 96% annuals and 4% perennial. Additionally, according to morphological classification, 59% were broad leaves, 28% were sedges and 13% were grasses. Different areas within Jeonnam province contained different numbers and occurrence rates of weed species. However, generally, we observed Lindernia dubia var. dubia, Monochoria vaginalis var. plantaginea, Ludwigia prostrata, L. procumbens, Cyperus difformis, Scirpus juncoides, Eleocharis Kuroguwai, Echinochloa oryzoides, and E. crus-galli var. echinata. We also observed seven weeds resistant to an ALS inhibiting herbicide. They were M. vaginalis, S. juncoides, C. difformis, L. dubia, Ludwigia prostrata, E. oryzoides, and E. crus-galli var. echinata. Although there were differences in the number and occurrence rate of resistant weed species to an ALS inhibiting herbicide among areas in Jeonnam province, the M. vaginalis, C. difformis, and S. juncoides occurred in 23 cities and counties in Jeonnam including Gwangju metropolitan city. Based on the rates (52%) of resistant occurrence to an ALS inhibiting herbicide in Jeonnam province, the area of weed resistant paddy fields was estimated to be 91,543 ha.

Ecological Traits of Weed Flora with Different Soil Moisture in Remodeled Paddy Field of Nakdong River (낙동강 리모델링 농경지 수분함량별 잡초발생 특성 분석)

  • Hwang, Jae-Bok;Yun, Eul-Soo;Jung, Ki-Yeol;Park, Chang-Young;Choi, Young-Dae;Jeon, Seong-Ho;Oh, In-Seok
    • Weed & Turfgrass Science
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    • v.2 no.1
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    • pp.8-14
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    • 2013
  • Collection of weeds were done in 3 remodeled paddy fields located in different districts with different soil moisture conditions such as Samolpo district (14.6%), Gimhae (9.8%) and Chilhyun (5.6%). Remodeled paddy fields composed of 62 plant species belonging to 23 families. Among 23 families, Compositae was the largest family with 11 species belonged to (17.7%) followed by Poaceae with 9 species (14.5%), Leguminosae with 8 species (12.9%), Cyperaceae with 7 species (11.3%), and Polygonaceae with 5 species (8.1%). Summed dominance ratio of each species based on number, coverage, frequency, and plant height in Samolpo district was Echinochloa oryzoides (100%) > Cyperus difformis (9.0%) > Bidens frondosa (5.3%) > Panicum dichotomiflorum (4.8%) > Eclipta prostrata (4.4%). In Gimhae; Digitaria ciliaris (100%) > Panicum dichotomiflorum (49.4%) > Echinochloa oryzoides (48.8%) > Persicaria hydropiper (27.1%) > Chenopodium ficifolium (10.2%), and in Chilhyun were Panicum dichotomiflorum (90.8%) > Digitaria ciliaris (66.7%) > Cyperus iria (8.6%) > Bidens frondosa (7.6%). Total dry weight of weeds recorded was 535.4 g $m^{-2}$, 316.1 g $m^{-2}$ and 346.2 g $m^{-2}$ in Samolpo, Gimhae and Chilhyun districts, respectively. Simpson's index was calculated to 0.09~0.28, which showed that weed occurrence in remodeled paddy fields with different soil moistures varied.

Effect of Brassinolide and Auxin Type Plant Growth Regulators on Radish Petiole Curvature (Brassinolide와 Auxin류(類)의 식물(植物) 생장조절제(生長調節劑)가 무우 자엽병(子葉柄)의 굴절(屈折)에 미치는 영향(影響))

  • Choi, C.D.;Takematsu, T.;Takeuchi, Y.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.84-89
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    • 1987
  • This study was conducted to determine the combining effects of a synthetic brassinolide such as homobrassinolide (HBR) with auxin types on the curvature of radish petiole. HBR has no direct effect on the petiole curvature of radish, regardless of concentrations used. Among the natural known plant growth regulators, NAA at 100ppm showed the highest ourvature at 12 hours after treatment. The synthetic auxins such as 2,4-D and MCP resulted in about three times greater effect on curvature than the untreated control, and their effects lasted till 36 horns after treatment, showing much better effect on curvature than that of the natural auxin types of plant growth regulators. The mixture of MCP at 3 ppm with HBR at 100ppm increased petiole curvature of radish seedlings as much as three times greater than that of a single application of HBR. However, the mixture of HBR at 100 ppm plus 2, 4-D at 100ppm increased the petiole curvature of radish seedlings about eight times greater than that of the HBR single application, showing existance of strong synergistic reaction and the most effective combination, and suggesting possibility of a prectical implementation in agriculture.

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Effects of Cover Crops and Sowing Methods on Weed Occurrences and Growth and Yield of Sorghum (Sorghum bicolor) (피복작물과 파종법에 따른 잡초발생과 수수의 생육 및 수량)

  • Jeon, Seung-Ho;Yun, Eul-Soo;Park, Chang-Young;Hwang, Jae-Bok;Jung, Ki-Youl;Choi, Young-Dae;Kim, Hyun-Ju;Shim, Sang-In
    • Korean Journal of Weed Science
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    • v.32 no.2
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    • pp.107-114
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    • 2012
  • This study was conducted to evaluate the weed suppressing effects of different cropping system including hairy vetch (Vicia villosa) and rye (Secale cereale) cover crops, polyethylene plastic film, and transplanting film mulching in direct sowing and transplanted sorghum field in 2011. Crop growth and development and weed occurrences in the fields were examined to know the efficiency of proposed cropping system. In polyethylene film mulching treatment, heading date of sorghum was earlier by 3 days than control, on the other hands, rye cover crop mulching delayed heading date by 11 days. Besides the effect of cover crop on the heading of sorghum, the residues changed growth characteristics. Plant height of sorghum was increased by 27.3% at hairy vetch treatment although it was reduced by 47.5% in the rye cover crop treatment. Hairy vetch treatment showed beneficial effects on sorghum growth reduced the occurrences of grasses and broadleaf weeds to 84% and 96%, respectively, as compared to control in sorghum fields. While rye cover crop treatment showed poor sorghum growth reduced less strongly grasses and broadleaf weeds by 35% and 71%, respectively. At harvest, yield of sorghum was greater in order of hairy vetch cover crop > polyethylene film mulching > rye cover crop ${\fallingdotseq}$ control in both transplanted and direct sown fields.

Effective Weed Control in Direct Seeded Rice on Puddled Paddy Surface of Southern Region (남부지역 무논직파 재배 논에서 잡초 방제)

  • Park, Tae-Seon;Park, Hong-Kyu;Kang, Sin-Koo;Ku, Bon-Il;Choi, Min-Kyu;Lee, Keong-Bo;Ko, Jae-Kwon
    • Korean Journal of Weed Science
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    • v.32 no.1
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    • pp.57-69
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    • 2012
  • This study was conducted to find the effective management of main weeds including herbicide-resistant Echinochloa oryzicola, Scirpus juncoides and Monochoria vaginalis in direct seeded rice on puddled paddy surface. The dominant dominances in direct seeded rice on puddled paddy surface were increased for three years between 2009-2011. In weed distribution in 2011, the occurrence density of S. juncoides decreased greatly, but it of E. oryzicola increased especially as compared with it of 2009. Benzobicyclon, mesotrione and pyrimisulfan were effective to sulfonylurea(SU)-resistant S. juncoides and M. vaginalis at 5 and 12 days after seeding, however, the efficacy of bromobutide to sulfonylurea-resistant M. vaginalis was decreased slightly at 12 days after seeding. And carfentrazon and pyrazolate were ineffective to S. juncoides. In direct seeded rice on puddled paddy surface which ACCase and ALS inhibitor-resistant E. oryzicola was not occurred, benzobicyclon+penoxsulam+pyrazosulfuron-ethyl ET, bromobutide+imazosulfuron+metamifop GR, and flucetosulfuron+mesotrione+pretilachlor GR showed the control effect over 94% to main weeds included sulfonylurea-resistant S. juncoides and M. vaginalis. The E. oryzicola dominating in direct seeded rice on puddled paddy surface were not any affected to the survival by treatment with recommended doses of cyhalofop-butyl and penoxsulam tested. The ACCase and ALS inhibitor-resistant E. oryzicola was effectively controlled by mefenacet and fentrazamide up to the 2nd leaf stage. In direct seeded rice on puddled paddy surface domiating coincidentally by herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis, "one-shot herbicide" included with mefenacet, fentrazamide, penoxsulam and metamifop could't be used because of low control effect or rice injury. The sequential treatment of benzobicyclon+thiobencarb SE and bensulfuron+mefenacet+thiobencarb GR controlled effectively the herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis in direct seeded rice on puddled paddy surface. The days required by the 3rd leaf stage of herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis in direct seeded rice on puddled paddy surface seeded in May 20 shortened by 4 days as compared with it of June 10.

Studies on Factors Affecting the Injury Caused by Simetryne to Rice Plants of Tongil Variety (제초제(除草劑) Simetryne제(劑에) 의한 통일(統一)벼의 약해발생요인(藥害發生要因) 구명(究明)에 관(關)한 연구(硏究))

  • Ryang, H.S.;Kim, I.G.
    • Korean Journal of Weed Science
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    • v.6 no.1
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    • pp.48-58
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    • 1986
  • In order to eluciate the factors affecting the injury caused by simetryne(2-methyl thio-4,6-bis(ethylamino)-S-triazine) to rice plants of Tongil variety(Indica ${\times}$ Japonica), the leaching properties of simetrynes, compared with Japonica varieties, Mangyeung and Sadominori were investigated. Bioassay technique and $^{14}C$-simetryne for leaching study and bioassay for the characteristics of the rice plants were employed. The leaching range of simetryne was very narrow regardless of soil types under upland soil conditions but it increased little in Silt clay soil and greatly in Sandy loam soil under flooded soil conditions. The amount of the roots of Tongil Jas a little more than that of Mangyeung and the roots of the Tongil mostly distributed near the surface soil while those of Mangyeung rooted deep into the soil. The foliage area and the rate of transpiration of Tongil were larger and greater than those of Mangyeung by 1.3 times and 1.65 times, respectively. The sensitivity of Tongil to simetryne was about 3 times larger than that of Sadominori in water culture.

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Antagonistic Mode of Action of Fenoxaprop-P-ethyl Phytotoxicity with Bentazon (Fenoxaprop-P-ethyl의 제초활성에 대한 Bentazon의 길항작용기구)

  • Ma, S.Y.;Kim, S.W.;Chun, J.C.
    • Korean Journal of Weed Science
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    • v.18 no.2
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    • pp.161-170
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    • 1998
  • Antagonistic mode of action of fenoxaprop-P-ethyl [ethyl(R)2-4-{(6-chloro-2-benzoxazolyloxy) phenoxy}propionate] with bentazon was investigated with respect to absorption, translocation, metabolism, and change in target site response of fenoxaprop-P-ethyl using four-leaf stage of rice(Oryza sativa L.) and barnyardgrass [Echinochloa eras-galli (L.) P. Beauv.]. Shoots of rice and barnyardgrass was more sensitive to fenoxaprop-P-ethyl than the roots. More than 90% of fenoxaprop-P-ethyl was absorbed within 6 hours after treatment and 30% of the absorbed was acropetally and basipetally translocated at 24 hours after treatment. Fenoxaprop-P-ethyl was rapidly transformed to its acid form, fenoxaprop(2-[4-(6-chloro-2-benzoxazolyloxy)phenoxy]propionic acid), which was subsequently metabolized to polar conjugates. However, changes in absorption, translocation, and metabolism of fenoxaprop-P-ethyl by bentazon treatment were not found in both species. Background activity of acetyl-CoA carboxylase(ACCase) in rice and barnyardgrass was 26.5 and 23.2nmol/min/mg, respectively. Concentration required to inhibit fifty percent enzyme activity$(I_{50})$ in vitro was 6.5~7.4${\mu}M$ of fenoxaprop-P-ethyl and more than 500${\mu}M$ of bentazon. There were no significant differences in $I_{50}$ value between two treatments of fenoxaprop-P-ethyl alone and its bentazon mixture. However, bentazon reduced ACCase activity in vivo and inhibited electron transport in chloroplast thylakoid. Based on the results obtained, it is concluded that the antagonistic effect of bentazon occurs due not to direct effect on target site of fenoxaprop-P-ethyl, but to indirect involvement in reducing herbicidal activity of fenoxaprop-P-ethyl through physiological disturbances caused by bentazone at whole chloroplast level.

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Effect of Tuber Size, Bud and Tuber Removal, Tuber Cutting on Growth of Eleocharis kuroguwai Ohwi (괴경(塊莖)의 크기 및 절제(切除)가 올방개의 생육(生育)에 미치는 영향(影響))

  • Kim, J.C.;Son, S.Y.;Yu, K.R.;Yoon, J.S.
    • Korean Journal of Weed Science
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    • v.9 no.1
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    • pp.28-33
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    • 1989
  • This study was examined for the effect of tuber size, bud and tuber removal, and tuber cutting on growth of Eleockaris kuroguwai Ohwi. Elongation of plumule and radicle, and the weights of tuber, rhizome and shoot were increased as tuber size is large. The weights of shoot and tuber reduced by bud planting without tuber at 15 days after budding, however number of tubers were increased compared with normal tuber. When cross-cutting tubers were seeded, the weights of shoot, rhizome and tuber were produced as 84%, 85%, 71%, respectively compared with normal tuber, and the number of tubers was not influenced. The weights of tuber, rhizome and shoot were lowed as the bud removal, and there were a great number of tubers at bud removal of 15 days after budding. Tuber of E. k, contained as nonfibrous 78.9gr, phosphorous 77mg, iron 16.8mg, niacin 1.3mg per 100gr edble portion. The positive correlations were observed between shoot weight and tuber weight, and number of tubers.

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Growth and Quality Changes of Creeping Bentgrass by Application of Keratin Amino Acid Fertilizer (케라틴 아미노산 비료 시비에 따른 크리핑 벤트그래스의 생육과 품질 변화)

  • Jo, Gi-Woong;Kim, Young-Sun;Ham, Soun-Kyu;Lee, Jae-Pil;Kim, Doo-Hwan;Kim, Woo-Sung;Lee, Geung-Joo
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.260-267
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    • 2016
  • Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.