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Physiology and Growth of Transgenic Tobacco Plants Containing Bacillus subtilis Protoporphyrinogen Oxidase Gene in Response to Oxyfluorfen Treatment (Bacillus subtilis Protoporphyrinogen Oxidase 유전자 형질전환 담배의 Oxyfluorfen 처리에 대한 생리 · 생장반응)

  • Lee, J.J.;Kuk, Y.I.;Chung, J.S.;Lee, S.B.;Choi, K.W.;Han, O.S.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.237-245
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    • 1998
  • The transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants containing Bacillus subtilis protoporphyrinogen oxidase gene with cauliflower mosaic virus 35S promoter have recently been generated by using Agrobacterium-mediated gene transformation. The nontransgenic and the transgenic tobacco plants were compared with respect to responses to diphenyl ether herbicide oxyfluorfen and under various environmental conditions. Both cellular leakage and lipid peroxidation caused by oxyfluorfen were found to be less in the transgenic than in the nontransgenic plants. Growth responses of the transgenic plants under various temperature, light, and water conditions were almost the same as those of the nontransgenic plants, although the transgenic plants exhibited slightly more retarded growth under low light or saturated water condition. These results revealed that the transgenic tobacco plants containing B. subtilis protoporphyrinogen oxidase gene under cauliflower mosaic virus 35S promoter were relatively resistant to oxyfluorfen and exhibited normal growth pattern. Possible mechanism of resistance to oxyfluorfen in the transgenic plants is also discussed.

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Research Review on Turfgrass Insect Pests in Korea (한국의 잔디해충 연구사)

  • Choo, Ho Yul;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.6 no.2
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    • pp.77-85
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    • 2017
  • Insect pests have been one of the main constraints affecting turf sod cultivation areas, playgrounds, parks, golf courses, airports and other related recreational grounds. However, turfgrass research has been very limited and confined within a limited period of time compared to other branches of crop science in Korea. This review was surveyed from all papers in KCI (Korea Citation Index), SCI (Scientific Citation Index) and SCIE (SCI Expended) journals related to turfgrass research in Korea. Fifty two papers concerning turfgrass insect pest in Korea have hitherto been published in 8 different scientific journals since 1990. Thirty three papers (63.5% of total) were published in Weed and Turfgrass Science. The main topics of focus were divided into two parts; ecological study and control of insect pests. Before the 1990 decade, there were no scientific papers published in relation to turfgrass insect pest science. However, during the 1990, 2000 and 2010 decades, 9, 10 and 14 papers were published respectively in Weed and Turfgrass Science. From 1997, about 2 papers were published per year in the scientific journals. Thirty three insect pest species belonging to 13 families in 6 orders, one Eriophyidae mite, Aceria zoysia and one Armadillidae sowbug, Armadillidium vulgare, have hitherto been listed as turfgrass arthropod pests in Korea. Fifty percent of turfgrass insect pest papers published were focused on whitegrub, Adoretus tenuimaculatus, Popillia quadrigutta and earthworm research in Korea.

Effect of Cover Crop on Weed Control in No-tilled Organic Soybean Field (콩 무경운 유기재배 시 피복식물의 잡초억제 효과)

  • Cho, Jung-Lai;An, Nan-Hee;Nam, Hong-sik;Lee, Sang-min;Ok, Jung-hun
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.189-195
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    • 2017
  • This study was conducted to evaluate weed control effect in organic soybean upland field as affected by cover crops including rye, hairyvetch, and its mixture. The experiment was conducted during two years (2015 and 2016) at the NAS (National Institute of Agricultural Sciences) organic farming experimental field. The cover crops were seeded after tillage at fall crop season in 2014, and then, the soybean field was managed with no tillage system from 2015. The weed suppression rates of cover crops application for rye and mixture (rye+hairyvetch) treatment during 60 days after transplanting were 80% and 30%, respectively. However, weed suppression rate of hairyvetch treatment was not significantly different as compared to control. Weed flora in experimental field were less than general soybean field. The average organic soybean yield was generally low compare to normal year, nevertheless, the soybean yield for rye and mixture treatment in 2016 were significantly higher than PE mulching treatment.

Herbicidal Activity of Essential Oil from Amyris (Amyris balsamifera) (아미리스 정유의 제초활성)

  • Yun, Mi Sun;Yeon, Bo-Ram;Cho, Hae Me;Choi, Jung Sup;Kim, Songmun
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.44-49
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    • 2012
  • The objective of this study was to know the herbicidal activity of the essential oil from amyris (Amyris balsamifera). In a seed bioassay experiment, the amyris essential oil inhibited the growth of rapeseed (Brassica napus) by fifty percent at 8.8 ${\mu}g\;g^{-1}$. And in a greenhouse experiment, sorghum, barnyard grass and Indian jointvetch, which was applied in above-ground parts, with the amyris essential oil at 4,000 ${\mu}g\;ml^{-1}$ showed visual injuries of 90, 70, and 70, respectively (0, no damage; 100, total damage). However, soil application of the essential oil did not show such herbicidal injuries. In a field experiment, foliar application of the amyris essential oil at 5% controlled effectively weeds such as barnyardgrass, shepherd's purse, and clover in 24 hours. Our results indicated that the amyris essential oil had herbicidal activity. To understand the composition of the amyris essential oil, the oil was analyzed by gas chromatography-mass spectometry with solid-phase micro-extraction apparatus. There were 15 organic chemicals in the oil and the major constituents were calarene, elemol, ${\gamma}$-eudesmol, curcumene, ${\beta}$-sesquiphellandrene, zingiberene, selina-3,7(11)-diene, 1,3-diisopropenyl-6-methyl-cyclohexene, ${\beta}$-bisabolene, and ${\beta}$-maaliene. Overall results suggest that the amyris essential oil had a herbicidal activity with fast, contact, and non-selective mechanism.

Molecular Identification of Pooideae, Poaceae in Korea (국내 농경지에 발생하는 포아풀아과 잡초의 분자생물학적 동정)

  • Lee, Jeongran;Kim, Chang-Seok;Lee, In-Yong
    • Weed & Turfgrass Science
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    • v.4 no.1
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    • pp.18-25
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    • 2015
  • A universal DNA barcoding for agricultural noxious weeds is a powerful technique for species identification without morphological knowledge, by using short sections of DNA from a specific region of the genome. Two standard barcode markers, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used to examine the effectiveness of the markers for Pooideae barcoding using 163 individuals of 29 taxa across 16 genera of Korean Pooideae. The rbcL and ITS revealed a good level of amplification and sequencing success while matK did not. Barcode gaps were 78.6% for rbcL, 96.2% for matK, and 91.7% for ITS, respectively. Resolving powers were 89.3% for rbcL, 92.3% for matK, and 79.1% for ITS. The matK obtained the best both barcode gap and resolving power. However, it should be considered not to employ matK for Pooideae barcode because of low rate of PCR amplification and sequencing success. As a single DNA marker, rbcL and ITS were reasonable for Pooideae barcode. Barcode gap and resolving power were increased when ITS was incorporated into the rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use.

Spreading and Distribution of Lactuca scariola, Invasive Alien Plant, by Habitat Types in Korea (침입외래식물 가시상추의 확산과 생육지 유형별 분포 특성)

  • Kim, Young-Ha;Kil, Ji-Hyon;Hwang, Sun-Min;Lee, Chang-Woo
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.138-151
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    • 2013
  • This study was carried out to investigate the distribution and habitat types of prickly lettuce, Lactuca scariola of Europe origin for supplying the basic data of management plan. It showed fairly nation-wide distribution although excessive growth was rather limited in the wild. Its habitat types were divided into four types like open fields, roadsides, seashores and riversides. Species diversity examined by species rank-dominance curve tended to increase over riverside > seashores > open fields > roadsides. As a result of analyzing life form, therophytes were more than 50%, means that the habitats of L. scariola were disturbed by human activities etc. Urbanization Index was analyzed 9.1% in roadsides, 7.4% in seashores, 5.8% in open fields and riversides. It has high spread potential with a large number of wind-flying seed per plant. It was evaluated that prickly lettuce was mainly spread along the newly constructed road, expressway and invaded the original ecosystem in the cultivated land as weeds. But it has played a role as a pioneer species in open fields. It is recommended to remove where it has considerable impact on the native plant species of conservation value.

A research on the Construction and Sharing of Authority Record-focusing on the Case of Social Networks and Archival Context Project (전거레코드 구축 및 공유에 관한 연구 SNAC 프로젝트 사례를 중심으로)

  • Lee, Eun Yeong
    • The Korean Journal of Archival Studies
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    • no.71
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    • pp.49-89
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    • 2022
  • This study suggests the necessity and domestic application plan a national authority database that promotes an integrated access, richer search, and understanding of historical information sources and archival resources distributed among cultural heritage institutions through the "Social Networks and Archive Context" project case. As the SNAC project was transformed into an international cooperative organization led by NARA, it was possible to secure a sustainable operating system and realize cooperative authority control. In addition, SNAC authority records have the characteristics of providing richer contextual information about life and history and social and intellectual network information compared to libraries. Through case analysis, First, like SNAC, a cooperative body led by the National Archives and having joint ownership of the National Library of Korea should lead the development and expand the scope of participating institutions. Second, in the cooperative method, take a structure in which divisions are made for each field with special strengths, but the main decision-making is made through the administrative team in which the two organizations participate. Third, development of scalable open source software that can collect technical information in various formats when constructing authority data, designing with the structure and elements of archival authority records, designing functions to control the quality of authority records, and building user-friendly interfaces and the need for a platform design reflecting content elements.

Weed Occurrence in Organic Apple Orchards by Different Cover Crops (유기재배 사과과원의 피복작물별 잡초발생)

  • Oh, Young-Ju;Song, Yang-Ik;Kang, Seok-Beom;Hong, Sun-Hee;Choi, Jin-Ho;Paik, Weon-Ki
    • Weed & Turfgrass Science
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    • v.1 no.4
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    • pp.24-30
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    • 2012
  • This study was conducted to select the cover crops in organic apple orchard by estimating the coverage of each cover crops and time-periodic weed occurrence. Seeding of Festuca myuros and Trifolium repens showed high contents of soil organic matter and phosphorus as compared with control. Seeding of Trifolium sp., Hordeum vulgare and Secale cereal reduced relatively occurrence of weed in May and S. cereal among others showed the greatest effect in June. In July, seeding F. arundinaceae and Poa pratensis showed low coverage and a lot of weeds occurred in their field. Fifty one species were occurred as weeds and it needs to removal of high and broadleaf weeds such as Chenopodium ficifolium, C. album var. centrorubru and Humulus japonicus in spring to increase the rate of ground cover. P. pratensis having the lowest cover rate showed the similar dry weight of weeds as compared with control. Trifolium sp. and Persicaria hydropiper had a high correlation with contents of soil organic matter and C. album var. centrorubru had a correlation with contents of soil phosphorus by CCA analysis.

Antibiotic Properties of Helicosporium sp. KCTC 0635BP to Rhizoctonia solani AG2-2 IV (Rhizoctonia solani AG2-2 IV에 대한 Helicosporium sp. KCTC 0635BP의 항균활성)

  • Lee, Sang Myeong;Kim, Dong Soo;Lee, Kwang-Soo;Lee, Chong-Kyu;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.202-206
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    • 2013
  • Biocontrol potential of an isolate of Helicosporium spp. against Rhizoctonia solani, Fusarium oxysporium and Phytophthora drechsleri was evaluated in vitro and in vivo. A selected biocontrol agent designated as Helicosporium 0635BP strongly inhibited growth and lysed mycelium of Rhizoctonia solani and Fusarium oxysporium on PDA. Autoclaved culture filtrate of the agent also completely inhibited growth of the turfgrass large patch pathogen, R. solani AG2-2 IV at the concentration of 50 ml $L^{-1}$. The pathogen was killed when dipped under the 20% filtrate for four hours or 50% for one hr. In a field trial, plots applied with the crude or times diluted culture filtrate showed 100% control efficacy of the turfgrass large patch as a chemical applied for a comparison. Results indicated that Helicosporium 0635BP is a promising biocontrol agent on control of the turfgrass large patch disease and its culture filtrate contained unknown heat suitable antifungal substance (s). Further studies on mass production, purification and identification of the unknown compound (s) are in progress for practical use.

Isolation of a New Herbicidal Compound Angelicin from Curly Dock (Rumex crispus L.) (소리쟁이(Rumex crispus L.)로부터 신규 살초활성물질 Angelicin의 분리)

  • Cho, Nam-Kyu;Lee, Sa-Eun;Choi, Jung-Sup;Hwang, Ki-Hwan;Koo, Suk-Jin;Wang, Hai-Ying;Kim, Song-Mun
    • Korean Journal of Weed Science
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    • v.30 no.3
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    • pp.183-190
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    • 2010
  • The objective of this study was to isolate a herbicidally active compound from curly dock (Rumex crispus), a native weed in Korea and to identify its' chemistry. The $GR_{50}$ value of methanol extracts which is determined by a seed bioassay using rapeseed (Brassica napus) seedlings was $935\;{\mu}g\;g^{-1}$. Activity-directed isolation of ethylacetate extract led to the isolation of ECDA fraction with $GR_{50}$ value $53\;{\mu}g\;g^{-1}$. Based on data of GC-MS, $^1H$-NMR and $^{13}C$-NMR, the chemical structure of ECDA was determined as 2H-furo[2,3-H]-[1]-benzopyran-2-one which is known as angelicin. The $GR_{50}$ values of angelicin to barnyardgrass (Echinochloa crus-galli), southern crabgrass (Digitaria ciliaris), and indian jointvetch (Aeschynomene indica) were 426, 66 and $216\;{\mu}g\;g^{-1}$, respectively. Our results suggest that angelicin could be used as a lead compound for the development of new herbicides.