• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.61-70
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• 2018

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.

• 대한기계학회논문집A
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• 제38권9호
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• pp.943-951
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• 2014

마이크로/나노 압입시험에 사용되는 각뿔 혹은 원뿔형 압입자의 선단 형상은 제작한계 및 사용 중 마모 등으로 인해 필수적으로 곡면 형태를 띄게 된다. 많은 압입시험 관련 연구에서 각뿔형 압입자의 선단 형상은 편의상 구형으로 가정한 후, 얕은 압입에 대한 구형압입 이론식을 적용하고 있다. 이러한 가정에는 근본적으로 두 가지 문제점이 있는데, 첫 번째로 이론해의 정확성은 재료 물성치 및 압입자 형상에 따라 변화한다는 점이며, 두 번째로 각뿔형 압입자의 실제 선단 형상은 이상적인 구형이 아니라는 점이다. 본 연구에서는 유한요소해석에 기반하여 압입시험에 미치는 이 두 요소의 영향을 분석한다. 먼저 탄성 구형 압입시험에 대해 푸아송비와 하중-변위 곡선의 상관관계를 살펴보고, 이를 기반으로 수정된 구형 탄성 압입 관계식을 제시한다. 이어 가정된 Berkovich 선단 형상의 3차원 유한요소해석으로부터 압입깊이에 따른 하중-변위 곡선의 특성을 분석한다.

• 대한기계학회논문집B
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• 제35권2호
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• pp.113-120
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• 2011

본 논문은 나노유체의 열확산율을 측정하는 센서와 주변회로 그리고 데이터의 처리방법을 제시한 것이다. 기존 비정상열선법을 이용하면 이론상 유체의 열전도율과 열확산율을 동시에 측정할 수 있으나 열전도율과 비교하여 열확산율은 많은 오차가 발생한다. 본 연구에서 제시한 방법은 측정변수가 단순하고 복잡한 센서의 교정과정을 생략할 수 있는 실용적 측면의 장점이 있다. 먼저 열확산율이 잘 알려진 유체들에 대한 검증실험을 실시하였고 나노유체의 열확산율을 측정하여 기본유체와 비교하는 과정을 예시적으로 설명하였다. 본 연구는 기존 열전도율측정에 한정되어 왔던 나노유체 연구의 범위를 열확산율 또는 비열의 개념으로 확장하였다는 관점에서 중요성을 갖는다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.879-894
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• 2016

In micro-fluid systems, fluids are injected into extremely narrow polymer channels in small amounts such as micro-, nano-, or pico-liter scales. These channels themselves are embedded on tiny chips. Various specialized structures in the chips including pumps, valves, and channels allow the chips to accept different types of fluids to be entered the channel and along with flowing through the channels, exert their effects in the framework of different reactions. The chips are generally crystal, silicon, or elastomer in texture. These highly organized structures are equipped with discharging channels through which products as well as wastes of the reactions are secreted out. A particular advantage regarding the use of fluids in micro-scales over macro-scales lies in the fact that these fluids are much better processed in the chips when they applied as micro-scales. When the laboratory is miniaturized as a microchip and solutions are injected on a micro-scale, this combination makes a specialized construction referred to as "lab-on-chip". Taken together, micro-fluids are among the novel technologies which further than declining the costs; enhancing the test repeatability, sensitivity, accuracy, and speed; are emerged as widespread technology in laboratory diagnosis. They can be utilized for monitoring a wide spectrum of biological disorders including different types of cancers. When these microchips are used for cancer monitoring, circulatory tumor cells play a fundamental role.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.146-153
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• 2006

A passive microfluidic delivery system using hydrophobic valving and pneumatic control was devised for microfluidic handling on a chip. The microfluidic metering, cutting, transport, and merging of two liquids on the chip were correctly performed. The error range of the accuracy of microfluid metering was below 4% on a 20 nL scale, which showed that microfluid was easily manipulated with the desired volume on a chip. For a study of the feasibility of biochemical reactions on the chip, a single enzymatic reaction, such as ${\beta}-galactosidase$ reaction, was performed. The detection limit of the substrate, i.e. fluorescein $di-{\beta}-galactopyranoside$ (FDG) of the ${\beta}-galactosidase$ (6.7 fM), was about 76 pM. Additionally, multiple biochemical reactions such as in vitro protein synthesis of enhanced green fluorescence protein (EGFP) were successfully demonstrated at the nanoliter scale, which suggests that our microfluidic chip can be applied not only to miniaturization of various biochemical reactions, but also to development of the microfluidic biochemical reaction system requiring a precise nano-scale control.

• 대한기계학회논문집A
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• 제34권11호
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• pp.1613-1622
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• 2010

본 연구는 고진공 환경중 초음파 리니어 스테이지의 나노미터 위치제어를 기술하고 있다. 고진공 환경 중 초정밀 위치 제어 시스템에 응용하기 위해 3 차 종진동 모드와 6 차 횡진동 모드를 가지는 BLT를 개발 했다. 안정적인 고출력을 위해 BLT 는 하나의 공진 주파수로 두 개의 모드 진동을 발생 시켜야 한다. 하나의 공진 주파수를 이용 하기 위해 어드미턴스를 변화시켜 각 모드의 공진 주파수를 일치시켜 조건이 다른 대기 환경에서 안정적인 고출력을 얻을 수 있었다. 기압 변화에 따라 구동 특성이 달라지는 시스템을 제어하기 위해 마찰력 변화에 따른 비선형 특성을 보상한 NCTF 제어를 사용했다. 설계된 제어기를 이용해 고진공 환경에서 시스템을 나노미터 정도로 제어하는 결과를 얻을 수 있었다.

• Journal of Electrical Engineering and Technology
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• 제13권5호
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• pp.2059-2066
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• 2018

This study explored the feasibility of utilizing an SWCNT-coated fabric sensor for the development of a wearable motion sensing device. The extent of variation in electric resistance of the sensor material was evaluated by varying the fiber composition of the SWCNT-coated base fabrics, attachment methods, number of layers, and sensor width and length. 32 sensors were fabricated by employing different combinations of these variables. Using a custom-built experimental jig, the amount of voltage change in a fabric sensor as a function of the length was measured as the fabric sensors underwent loading-unloading test with induced strains of 30 %, 40 %, and 50 % at a frequency of 0.5 Hz. First-step analysis revealed the following: characteristics of the strain-voltage curves of the fabric sensors confirmed that 14 out of 32 sensors were evaluated as more suitable for measuring human joint movement, as they yield stable resistance values under tension-release conditions; furthermore, significantly stable resistance values were observed at each level of strain. Secondly, we analyzed the averaged maximum, minimum, and standard deviations at various strain levels. From this analysis, it was determined that the two-layer sensor structure and welding attachment method contributed to the improvement of sensing accuracy.

• 한국정보통신학회논문지
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• 제17권8호
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• pp.1926-1933
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• 2013

무선 센서 네트워크를 위한 다양한 위치 추적 알고리즘들이 제안되고 있다. IEEE 802.15.4a 기반의 위치인식 시스템은 두 노드간의 정밀한 거리측정 기능을 제공하며 이를 기반으로 정확도가 높은 위치 추정 서비스를 제공한다. 하지만 실내 환경에서는 다양한 장해물들로 인하여 Non-line-of-sight (NLOS) 경로가 발생한다. 이로 인하여 IEEE 802.15.4a 기반의 위치 인식 시스템에서는 위치 추정 시 추정된 위치 좌표의 정확도가 떨어지는 문제가 발생한다. 이를 해결하기 위하여 본 논문에서는 MST 토폴로지를 이용한 실내 환경에서의 위치측정에러를 보상하는 알고리즘을 제안한다. NanoPAN 5375 모트를 이용한 실내 환경에서의 실험 및 시뮬레이션 결과, 제안한 알고리즘은 기존에 제안된 위치 추정 알고리즘에 비하여 위치 정확도가 개선되었음을 확인하였다.

• 한국운동역학회지
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• 제31권3호
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• pp.183-188
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• 2021

Objective: The purpose of this study was to develop a stretchable joint motion sensor that is based on silver nano-particle. Through this sensor, it can be utilized as an equipment for rehabilitation and analyze joint movement. Method: In this study, precursor solution was created, after that, nozel printer (Musashi, Image master 350PC) was used to print on a circuit board. Sourcemeter (Keithley, Keithley-2450) was used in order to evaluate changes of electric resistance as the sensor stretches. In addition, the sensor was attached on center of a knee joint to 2 male adults, and performed knee flexion-extension in order to evaluate accurate analysis; 3 infrared cameras (100 Hz, Motion Master 100, Visol Inc., Korea) were also used to analyze three dimensional movement. Descriptive statistics were suggested for comparing each accuracy of measurement variables of joint motions with the sensor and 3D motions. Results: The change of electric resistance of the sensor indicated multiple of 30 times from initial value in 50% of elongation and the value of electric resistance were distinctively classified by following 10%, 20%, 30%, 40% of elongation respectively. Through using the sensor and 3D camera to analyze movement variable, it showed a resistance of 99% in a knee joint extension, whereas, it indicated about 80% in flexion phase. Conclusion: In this research, the stretchable joint motion sensor was created based on silver nanoparticle that has high conductivity. If the sensor stretches, the distance between nanoparticles recede which lead gradual disconnection of an electric circuit and to have increment of electric resistance. Through evaluating angle of knee joints with observation of sensor's electric resistance, it showed similar a result and propensity from 3D motion analysis. However, unstable electric resistance of the stretchable sensor was observed when it stretches to maximum length, or went through numerous joint movements. Therefore, the sensor need complement that requires stability when it comes to measuring motions in any condition.

• Advances in nano research
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• 제7권3호
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• pp.191-208
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• 2019

In the present work the dynamic analysis of the functionally graded rectangular nanoplates is studied. The theory of nonlocal elasticity based on the quasi 3D high shear deformation theory (quasi 3D HSDT) has been employed to determine the natural frequencies of the nanosize FG plate. In HSDT a cubic function is employed in terms of thickness coordinate to introduce the influence of transverse shear deformation and stretching thickness. The theory of nonlocal elasticity is utilized to examine the impact of the small scale on the natural frequency of the FG rectangular nanoplate. The equations of motion are deduced by implementing Hamilton's principle. To demonstrate the accuracy of the proposed method, the calculated results in specific cases are compared and examined with available results in the literature and a good agreement is observed. Finally, the influence of the various parameters such as the nonlocal coefficient, the material indexes, the aspect ratio, and the thickness to length ratio on the dynamic properties of the FG nanoplates is illustrated and discussed in detail.