• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2003년도 추계학술발표대회 논문요약집
• /
• pp.35-35
• /
• 2003

We, for the first time, reported molecular sequences of large subunit ribosomal DNA Dl-D3 region of A. hiranoi, A. leei and A. satoanum hitherto unreported. In addition, this study presented the full-length sequences of A. affine, A. fraterculus, A. catenella and A. tamarense occurring in Korean coastal waters. In total, 17 Alexandrium morphospecies were subjected to the phylogenetic analysis using the Maximum-likelihood (ML) method. The alignment result of sequences of A. hiranoi and A. pseudogonyaulax showed that there were only two substitutions without length heterogeneity implying their genetic affiliation. In ML tree, A. leei formed a deeply diverging branch probably because of the accelerated evolutionary rate, and its phylogenetic position was so ambiguous to resolve the phylogenetic relationship to the residual taxa. An A. satoanum culture showing morphological variation in the sulcal plate formed an independent divergent branch with consistent sister relationship to A. hiranoi/A. pseudogonyaulax clade supported by the high posterior probability (PP) value. Blast search in GenBank showed the sequence data of A. affine, A. fraterculus, A. catenella and A. tamarense corresponded to their morphological species designation. In ML tree, Alexandrium species were commonly split into four main clades. The inter-clade relationships were not clear and usually supported by the week PP values. In general, the sulcal plate of Alexandrium species seemed to reflect the true phylogeny at the main clade level, and the connection between the 1 and the apical pore complex seemed to reflect the phylogeny at the subclade level.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권4호
• /
• pp.2599-2605
• /
• 2013

The study aimed to evaluate immune-stimulating effects of a well-known Thai folkloric remedy when used for adjuvant therapy with conventional chemotherapeutics for treatment of breast cancer. Immunostimulating influence of the remedy (215 mg/kg body weight per day) on NK cell activity and TNF-${\alpha}$ release from the monocytes/macrophages were investigated in a total of 15 healthy women and 13 female patients with breast cancer (Group 1). The effect of breast tumor surgery on NK cell activity was further investigated in 18 female patients with breast cancer (Group 2). NK cell cytotoxic activity was determined by chromium release cytotoxic assay using K562, an erythroleukemic cell line. TNF-${\alpha}$ release from monocytes/macrophages separated from blood samples was determined through a biological assay using actinomycin D-treated L929 mouse fibroblast cells in the presence and absence of LPS. Baseline NK cell activity of the monocytes/macrophages separated from Group 2 patients expressed as %cytotoxicity was significantly lower than in the healthy subjects at E:T ratios of 100:1 and 25:1. In healthy subjects, there was no change in NK cell cytotoxic activity (%cytotoxicity or LU) following 1 and 2 weeks of treatment with the remedy compared with the baseline at various E:T ratios but the binding activity (%binding) was significantly increased after 2 weeks of treatment. The addition of one or two conventional chemotherapeutic regimens did not significantly reduce the NK cytotoxic activity but did affect release of TNF-${\alpha}$ in both unstimulated and LPS-stimulated samples. Surgery produced a significant suppressive effect on NK cell activity. The use of the remedy as an adjunct therapy may improve therapeutic efficacy and safety profiles of conventional chemotherapeutic regimens through stimulation of the immune system in cancer patients.

• 한국경제지리학회지
• /
• 제14권4호
• /
• pp.603-615
• /
• 2011

최근 지역 활성화정책의 일환으로 관광의 중요성이 날로 강조되고 있는 가운데, 한국인관광객의 비중이 약 60%를 차지하고 있는 일본 큐슈 지역에서는 한국인관광객을 겨냥한 국제교류 축제 및 이벤트가 활발히 개최되고 있다. 그 이유로는 첫째, 큐슈 지역이 타 국가들보다도 한국과 가장 가깝고 교통이 편리하다는 점, 둘째, 큐슈 지역에는 한일양국의 국제교류의 소재가 될 만한 한국관련 역사가 풍부하다는 점, 셋째, 큐슈지역은 일본 내에서도 특히 한국과 자매결연을 맺고 있는 시정(市町)의 수가 많아, 국내 자매도시들과의 국제교류행사가 용이하다는 점, 등을 들 수 있다. 특히 큐슈 지역 내에서도 나가사키현 쓰시마시는 이상의 조건들을 가장 만족시키는 지역이라는 점에서, 한국과의 국제교류를 이용한 지역 활성화의 움직임이 가장 적극적인 것으로 분석된다.

• BMB Reports
• /
• 제48권1호
• /
• pp.36-41
• /
• 2015

Recently, we demonstrated that human serum amyloid P component (SAP) specifically recognizes exposed bacterial peptidoglycan (PGN) of wall teichoic acid (WTA)-deficient Staphylococcus aureus ${\Delta}$tagO mutant cells and then induces complement-independent phagocytosis. In our preliminary experiments, we found the existence of human serum immunoglobulins that recognize S. aureus PGN (anti-PGNIgGs), which may be involved in complement-dependent opsonophagocytosis against infected S. aureus cells. We assumed that purified serum anti-PGN-IgGs and S. aureus ${\Delta}$tagO mutant cells are good tools to study the molecular mechanism of anti-PGN-IgG-mediated phagocytosis. Therefore, we tried to identify the intracellular molecule(s) that is involved in the anti-PGN-IgG-mediated phagocytosis using purified human serum anti-PGN-IgGs and different S. aureus mutant cells. Here, we show that anti-PGN-IgG-mediated phagocytosis in phorbol myristate acetate-treated U937 cells is mediated by $Ca^{2+}$ release from intracellular $Ca^{2+}$ stores and anti-PGN-IgGdependent $Ca^{2+}$ mobilization is controlled via a phospholipase C${\gamma}$-2-mediated pathway.

• 대한지리학회지
• /
• 제43권6호
• /
• pp.951-960
• /
• 2008

본 연구에서는 쓰시마시의 아리랑 축제를 사례로, 쓰시마시는 어떠한 과정을 거천 아리랑 축제를 한국인을 겨냥한 장소마케팅의 수단으로 이용해 왔는지, 아리랑 축제의 역사를 통한 민과 관의 활동에 착안하여 고찰하였다. 쓰시마시는 기존의 축제에 조선통신사 행렬과 아리랑 축제의 타이틀을 추가함으로써, 한국인 관광객을 유치하려고 노력하여 왔으나, 독도문제 등으로 인한 일부 주민들의 항의에 부딪혔다. 이에 시는 협의회를 거쳐, 지역 활성화를 위해 축제를 중지할 필요는 없지만, 2007년부터는 아리랑 축제를 서브타이틀로 사용할 것에 합의하였다. 이처럼 지역주민들은 때로는 아리랑 축제의 개최를 둘러싸고 관과 첨예하게 의견대립을 보이는 경우도 있었지만, 한편으로 그들은 축제의 운영에서 프로그램의 공연에 이르기까지 적극적으로 참가하고 있어, 쓰시마시의 아리랑 축제는 지역주민의 아이덴티티에 기초한 그들의 축제이면서 동시에 장소마케팅의 수단으로 이용되고 있음을 알 수 있고, 그 과정은 민과 관이 함께 만들어 가는 지역 만들기의 과정이라고 할 수 있다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권4호
• /
• pp.261-266
• /
• 2004

Hyperthermia (HT) in combination with anticancer drugs (ACDs) had proven to more efficacious in various cancers, although efficacies vary according to chemotherapeutic compounds and cancer types. Presently there are few data that compares anticancer efficacies among ACDs under hyperthermic conditions. Therefore, we selected three commonly used ACDs (quercetin, verapamil and doxorubicin) and compared their antitumor effects when each was treated with 43$^{\circ}C$ HT exposure. Firstly, FM3A, a murine breast cancer cell line, was treated with each ACD for 1 h followed by 43$^{\circ}C$ exposure for additional 1 h, and examined the effects of: 1) each drug, 2) 43$^{\circ}C$ HT exposure, and 3) the combination of each drug and 43$^{\circ}C$ HT exposure for 1, 6 and 24 h. The determined overall effects on FM3A cells were arrested cell proliferation, clonogenic efficiency and apoptosis. Pre-treatment of FM3A cells to each ACD followed by 43$^{\circ}C$ HT exposure produced greater antitumor effects including suppressed cell proliferation, reduced clonogenic efficiency and increased apoptotic cell death, compared to ACD treatment or HT exposure alone. Apoptotic cell death occurred in a time-dependent manner. Among the ACDs, antitumor efficacies varied in the order of doxorubicin > verapamil > quercetin. It was concluded that heat exposure during ACD treatment of caner cells may be an important factor to get a better antitumor benefit, even though this benefit may differ from one drug to another.

• 한국콘텐츠학회논문지
• /
• 제10권9호
• /
• pp.126-136
• /
• 2010

본 연구는 일본의 공영방송인 NHK의 역사드라마 콘텐츠가 가져다주는 경제적 효과'에 주목하고 있다. 연구결과, NHK의 역사드라마는 일본의 지역경제 발전에 결정적인 역할을 하는 것으로 나타났다. 일본 은행의 분석에 의하면 2010년 작품인 '료마덴'은 드라마의 무대인 고치현과 나가사키현 등에 무려 6127억원의 경제효과를 가져다주는 것으로 나타났다. 이는 드라마가 큰 인기를 끌면서 지역을 찾는 관광객이 크게 늘어난데 기인한 것이다. NHK는 또 같은 시기에 특정지역을 배경으로 한 드라마 3편을 집중적으로 방송함으로써 해당 지역에 대한 관심도를 부각시키는 전략도 사용하고 있다. NHK의 이런 노력은 제조업계와 서비스업계에도 긍정적인 영향을 미쳤다. 국민이 낸 수신료를 통해 운영되는 NHK는 역사드라마 콘텐츠를 통해 '지역경제발전'을 꾀하는 등 방송의 공공성을 극대화하기 위해 노력하고 있는 것으로 평가되고 있다.

• World Technopolis Review
• /
• 제1권1호
• /
• pp.56-64
• /
• 2012

This paper provides a case study to explore the Kitakyushu case as a good referential example on STP development for rejuvenation of declining industrial city. The major data for the case, basically, has been sourced from some materials published by the municipality of Kitakyushu City, the website of the Kitakyushu Science and Research Park (KSRP) etc. The City of Kitakyushu has promoted the KSRP development to overcome industrial decline and stimulate city renaissance as a new industrial city. The core of the development was to develop a high-level education and research environment by gathering several HEIs into one campus. Based on the environment, the Kitakyushu Foundation for the Advancement of Industry and Science and Technology (FAIS) played the critical role as a coupler to make various networks and boost industry-academiaadministration innovation. The KSRP, and then, has been designated as a project area of some cluster projects initiated by the central government. It means that local projects can be synchronized, synergized and synthesized with national projects in the KSRP. In addition, through a series of the environmental approach from the Kitakyushu Eco-town Project to the Low Carbon Society Project, the development of the KSRP is being extended into the region. In the Kitakyushu case, networking is the essence of the KSRP development from the planning stage. First of all, the plan put emphasis on networking of academia for the knowledge creation based on competitive collaboration that is expected as the mainspring of rejuvenating declining industrial area and making a new industrial city that the plan aimed. Then, the roles of two organizations are very important for networking, especially networking of networks: the Campus Management Committee as an interface and the FAIS as a coupler. STP development without some strategic considerations for networking as the way of interaction among its participants cannot be a tool to promote innovation and rejuvenate a declining industrial area. The Kitakyushu case mentioned above explains the matter clearly.

• 한국수산과학회지
• /
• 제40권2호
• /
• pp.79-83
• /
• 2007

The maturation and spawning of Shuttles hoppfish Periophthalmus modestus were studied from April to October, 2005 in the mud flat of Suncheon Bay, Korea. The specimens ranged in body length (BL) from 1.5 to 8.4 cm. The gonadosomatic index (GSI) of females was highest in May and decreased until September. The hepatosomatic index (HSI) and fatness index of females were high in April and then decreased to a nadir in July. The data indicate that the spawning season was from April to September. There was no significant difference in the ratio of females to males $(X^2-test,\;p>0.01)$. Over 50% of the females over 5 cm in BL were sexually mature. Fecundity ranged from 1,664 to 13,428. The relationship between fecundity (F) and body length (BL) of the fish was expressed as $F=0.026BL^{2.8638}\;(R^2=0.79)$. The first spawning length was 4.5 cm BL.

• 한국수산과학회지
• /
• 제45권1호
• /
• pp.17-24
• /
• 2012

We cloned and sequenced the cDNA encoding the $FSH{\beta}$ and $LH{\beta}$ subunits from the pituitary of the blacktip grouper Epinephelus fasciatus, which regulate vitellogenesis and maturation in vertebrates, to achieve stable and healthy gametes. The full-length cDNAs of $FSH{\beta}$ and $LH{\beta}$ were 571 bp and 617 bp, encoding 120 amino acid (aa) and 147 aa proteins, respectively. The deduced amino acid sequences of $FSH{\beta}$ and $LH{\beta}$ were highly homologous (68-97%) to those of other Perciformes; E. bruneus, Dicentrarchus labrax, Thunnus thynnus, and Pseudolabrus sieboldi. Phylogenetic analysis showed that the deduced $FSH{\beta}$ and $LH{\beta}$ amino acid sequences were categorized as a distinct subunit in the $GTH{\beta}$ family, and are closely related to the teleostei $FSH{\beta}$ and $LH{\beta}$, respectively. $FSH{\beta}$ and $LH{\beta}$ mRNA exhibited high abundance in the pituitary gland and low in other brain areas, but were not present in peripheral tissues, as determined by RT-PCR.