• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.4
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• pp.391-400
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• 2015

In the present study, 50% ethanol extract, the ethyl acetate and aglycone fraction were prepared from mate (Ilex paraguariensis) and their antioxidative ability was evaluated. The yields of extract and fractions were 32.0, 4.48 and 0.82% per dried powder, respectively. Free radical scavenging activities were performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and total antioxidant capacity was estimated using luminol-dependent chemiluminescence assay. Free radical scavenging activities ($FSC_{50}$) of 50 % ethanol extract, ethyl acetate fraction and aglycone fraction were 8.83, 5.84 and $6.05{\mu}g/mL$, respectively. Their total antioxidant capacities ($OSC_{50}$) were similar to that of L-ascorbic acid ($1.72{\mu}g/mL$), known as a prominent water soluble antioxidant, in all extracts and 50% ethanol extract ($1.03{\mu}g/mL$) was the most effective. The cellular protective effects on the $^1O_2$-induced cellular damage of erythrocytes were evaluated and the results showed that all extracts were significantly higher than (+)-${\alpha}$-tocopherol at $10{\mu}g/mL$. Especially, the ${\tau}_{50}$ value of aglycone fraction was 5 times higher than (+)-${\alpha}$-tocopherol at $10{\mu}g/mL$ and $50{\mu}g/mL$. The inhibitory effects of the ethyl acetate and aglycone fractions on tyrosinase were similar to arbutin, known as the whitening agent in cosmetics. These results suggest that the extracts of mate have the applicability as antioxidant and anti-aging cosmeceutical ingredients.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.4
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• pp.325-331
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• 2015

Skin is exposed to sunlight or artificial indoor light on a daily. The reached solar light on the earth surface consist of 50% visible light and 45% infrared (IR) except for ultra violet (UV). The negative effects of UV including UVB and UVA have been steadily investigated within the last decades. However, little is known about the effects of visible or IR light. In this study, we irradiated human dermal fibroblasts using light emitting diode (LED) to investigate the optimal parameter for enhancing cell growth and collagen synthesis. We found that red of 630 nm and green of 520 nm enhance the cell proliferation, but irradiation with purple and blue light exerts toxic effects. To examine the response of irradiation time and light intensity on the fibroblasts, cells were exposed to red or green light with intensities from 0.05 to $0.75mW/cm^2$. Procollagen secretion was increased of 1.4 fold by 10 min irradiation, while 30 min treatment decreased the collagen synthesis of dermal fibroblasts. Treatment with red of $0.3mW/cm^2$ and green of 0.15 and $0.3mW/cm^2$ resulted in enhancement of collagen mRNA. Lastly, we investigated the combinatorial effect of red and green light on dermal fibroblasts. The sequential irradiation of red and green light is an efficient way for the purpose of the increase in the number of fibroblasts than single light treatment. On the other hand, the exposure of red light alone was more effective method for enhancing of collagen secretion. Our study showed that specific light parameters accelerated cell proliferation, gene expression and collagen secretion on human dermal fibroblasts. In conclusion, we demonstrate that light exposure with specific parameter has beneficial effects on the function of dermal fibroblasts, and suggests the possibility of its cosmetically and clinical application.

• Food Science and Preservation
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• v.15 no.5
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• pp.635-641
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• 2008

This study was conducted to investigate the optimal sub-zero temperature for storage of two garlic cultivars, the northern ecotype 'Jaerae' and the southern ecotype 'Daeseo'. These cultivars of garlic bulbs were stored at different temperature (room temperature, $0^{\circ}C$, and $-4^{\circ}C$) conditions after predrying. Southern ecotype garlic showed higher weight loss than northern ecotype garlic. Northern ecotype garlic at $-4^{\circ}C$ exhibited less than 5% of weight loss during 10 month storage. Sprouting rate was higher in southern than in northern ecotype at room temperature storage. The value of injury by disease and insect was similar between southern ecotype and northern ecotype for 5 month storage, and then northern ecotype showed higher value of injury by disease and insect than southern ecotype. Hunter 'b' value of northern ecotype garlic was the lowest at $-4^{\circ}C$ storage. Northern ecotype garlic showed no cold injury at freezing point temperature storage. However, southern ecotype garlic had $0{\sim}5.3%$ cold injury occurrence at $-4^{\circ}C$ storage. Respiration and ethylene production exhibited the highest at room temperature storage, those had the slight increase at $0^{\circ}C$ and $-4^{\circ}C$ storages. Northern ecotype showed higher enzymatic pyruvic acid and fructan contents than those of southern ecotype. Enzymatic pyruvic acid content increased and fructan contents decreased during storage time.

• Food Science and Preservation
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• v.19 no.6
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• pp.825-832
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• 2012

In this study, the quality characteristics and antioxidant activity of mulberry salad dressing prepared with yam juice during storage at $5^{\circ}C$ for four weeks were evaluated. The salad dressing was prepared with a salad base, mulberry, omija, and yam (4:4:6:1 or 4:4:6:3). Fresh yam juice was added to the salad dressing at 0, 7, and 18% levels. After four weeks storage, the pH increased whereas the acidity decreased. As the yam concentration increased, the pH increased to 3.84 and the acidity decreased to 0.14%. The Hunter color L (lightness), b (yellowness), and a (redness) values decreased over the storage period. After four weeks storage, the viscosity increased from 83.2 to 158.5 cp according to the amount of yam juice that was added. The antioxidant activity, such as the DPPH radical scavenging activity, and the total polyphenol content of the salad dressing improved as the yam concentration increased. Moreover, according to the storage time, the peroxide value did not increase. These results show that the mulberry salad dressing to which omija and fresh yam juice were added maintained its freshness with high antioxidative activity during storage.

• Food Science and Preservation
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• v.17 no.5
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• pp.652-658
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• 2010

This study was to investigate physicochemical properties of Shinan seomcho growing in Jeollanamdo. The samples used were Shinan seomcho(Josaeng, Mansaeng varieties), Pohangcho(Josaeng, Mansaeng varieties), Namhaecho (Josaeng, Mansaeng varieties) and greenhouse spinach(Josaeng varieties). The results were as follows ; The thickness of spinach leaves were contained in order of Shinan seomcho > Pohangcho > Namhaecho > greenhouse spinach, and the hardness of those were contained in order of Pohangcho > Namhaecho > Shinan seomcho > greenhouse spinach. In Hunter's color value, L, a, b value in greenhouse spinach was lower than those of the others. Among the spinach varieties, Josaeng-jong(3.39~5.38 mg%) had the highest level of total dietary fiber contents than that of Mansaeng-jong(2.94~3.91 mg%). By regional groups, total dietary fiber were contained in order of Pohangcho > Namhaecho > Shinan seomcho > greenhouse spinach. The free sugar contents in the sample group, Shinan seomcho(4 varieties), Pohangcho(1 variety) and Namhaecho(2 varieties) were higher than those of other spinach varieties. The total sugar contents in the sample group, Shinan seomcho(3 varieties), Pohangcho(1 variety) and Namhaecho(2 varieties) were higher than those of other spinach varieties. Total flavonoid contents of Shinan seomcho(Josaeng-jong) and Namhaecho(Josaeng-jong) were lower than 130 mg%, but the other spinach had more than 130 mg% of total flavonoid contents. Germanium contents were detected in the following order ; Shinan seomcho(47.46 ${\mu}g$/kg) > Namhaecho(30.13 ${\mu}g$/kg) > Pohangcho(26.39 ${\mu}g$/kg) > greenhouse spinach(25.32 ${\mu}g$/kg).

• Food Science and Preservation
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• v.24 no.3
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• pp.464-471
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• 2017

The purpose of this study was to evaluate the effect of superheated steam drying on physicochemical and microbial characteristics of Korean traditional actinidia (Actinidia arguta) leaves. Actinidia leaves were dried at steam temperature of $350^{\circ}C$ and oven temperature of $150^{\circ}C$ for 40-200 sec. Moisture content and water activity decreased with increasing the drying time, while color values including L, a, and b values and total color difference (${\Delta}E$) increased as drying time increased. The relationship between moisture content and water activity showed an exponential fit with high correlation vlaue ($R^2=0.9909$). Total phenolics and flavonoids content and antioxidant activity such as DPPH radical scavenging activity, ABTS radical scavenging activity, and FRAP assay of dried actinidia leaves increased with increasing the drying time up to 160 sec, but dramatically decreased at drying of 200 sec. The numbers of total areobic bacteria of leaves was not detected at drying time over 120 sec and coliform of all the samples was not detected. As a results, the superheated steam was an very effective drying method of increase to the nutritional and sanitary quality of dried Korean traditional actinidia leaves.

• Food Science and Preservation
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• v.25 no.1
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• pp.136-144
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• 2018

This study aimed to provide basic evidence regarding the development of materials by analyzing the physicochemical properties and antioxidant activities of Streptococcus thermophilus KCCM 3782 strain-fermented Cordyceps militaris grown on Tenebrio molitor. Among the solvents tested, DPPH radical scavenging activity was the highest in the hot water-extracted sample after 30 min of extraction. Moisture content decreased, whereas crude protein and fat content increased, after lactic acid bacteria-mediated fermentation. Sodium, magnesium, calcium, and zinc contents increased, with potassium levels attaining the maximum value, whereas free amino acid content decreased after the fermentation. Among Hunter's color values, a value increased to $66.7{\rightarrow}149.92$ after fermentation, whereas the L and b values decreased to $15.79{\rightarrow}-15.75$ and $54.45{\rightarrow}0.01$, respectively. Cordycepin content assay increased from 7.02 mg% to 8.66 mg%. The DPPH free radical scavenging activity of the fermented product was 91.92 EDA%, which was higher than that of the extract (84.69 EDA%). The ABTS free radical scavenging and superoxide dismutase (SOD) activities were also higher in the fermented products.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.353-360
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• 2011

In order to develop the application method of pig compost (PC) and liquid manure (LM) for whole crop barley cultivation, experiments were conducted at Munpo series (coarse loamy, mixed, nonacid, mesic family of Typic Fluvaquents) soil in Gyehwa-reclaimed land, six plots, a LM applied rate as N% ; non-application, chemical fertilizer (CF)100, 100, 50+50, 50+CF50 and (PC30+LM40)+LM50 as basal and additional fertilizer. $NO_3^-$-N content in soil was decreased as along with the growth of plant, highest in LM100% as basal fertilization at early growth stage and highest in (PC30%+LM40%)+LM40% and CF100% at last growth stage. Amount of $NO_3^-$-N and $NH_4^+$-N in soil was high in (PC30%+LM40%)+LM40% and CF100% of top soil but in subsoil significant difference was little in all treatment. Amount of OM, $A_V.P_2O_5$, T-N, exchangeable Ca and Na in soil was higher (PC30%+LM40%)+LM40% than non-application after harvest. Amount of nutrient uptake in plant was higher in CF100% and split application of LM than LM 100% application. Nitrogen utilization rate was in the order of CF100% >LM50%+LM50%=LM50%+CF50%>(PC30%+LM40%)+LM40% >LM100%. The yield of whole crop barley in (PC30%+LM40%)+LM40% and CF100% was 3.2 times more than in non-application ($309kg\;10a^{-1}$). Feed values such as crude protein and TDN was increased 1.0% ~ 1.4% in LM as split application than basal 100% treatment. Accordingly, in order to increase yield of a whole crop barley with application PC+LM in reclaimed land treat split application rather than to treat LM 100% into the land.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.361-367
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• 2011

Soil fertility of alpine soils in Gangwon-Do has been deteriorating because of heavy input of chemical fertilizers for intensive crop production. To reduce application of chemical fertilizers, use of livestock manure compost in alpine soils increases consistently. Soil loss and runoff due to heavy rainfall in alpine area cause nutrient loss from soil, and subsequently pollute stream water. Therefore, the objective of this study was to assess nutrient efficiency and loss in Chinese cabbage cultivated soil with different livestock manure composts in several slopes. As control, chemical fertilizer was applied at the rate of $250-78-168kg\;ha^{-1}$ for $N-P_2O_5-K_2O$. Each pig-and chicken manure compost was applied at the rate of $10MT\;ha^{-1}$. Chemical fertilizer + chicken manure compost was applied as same rate. Four treatments was practiced in 5, 20, and 35% filed slopes, respectively. We monitored the amounts of soil loss and runoff water after rainfalls, and we also analyzed the contents of nutrients in soil and runoff water through lysimeter installed in alpine agricultural institute in Gangwon-Do. T-N loss due to soil loss was much greater with increasing filed slops rather than different fertilizer treatments. T-N loss has positive relationship with field slopes, which showing soil loss (MT/ha) = 1.66 slopes (%) - 3.5 ($r^2$ = 0.99). Available phosphate and exchangeable cations showed similar tendency with increasing slopes. T-N and T-P losses caused by runoff water were highest in chemical fertilizer (NPK) + chicken manure compost treated plot, while lowest in chemical fertilizer treatment. T-N contents (2.13, 1.95%) in chinese cabbage treated either pig and chicken manure composts compared to that (2.65%) of chemical fertilizer were significantly less. This could be resulted from much greater T-N loss in soil treated with pig and chicken manure composts.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.1018-1023
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• 2010

Climatic change was observed and analyzed in view of impacts on agricultural ecosystem, inter alia on rice cropping. The changed climate gave rise to earlier transplanting of rice seedling and later harvest after 40 years. Also phenological change and prolonged growth duration was observed. The meteorological data was selected from the standardized climatological data of 30 year normals of 1960s and 2000s, which were published by Korea Meteorological Administration. Development stages and growing periods of rice crop were compared by analyzing critical and optimum temperatures of each growth stage during these two periods. The first appearance date of $15^{\circ}C$ was ranged from Apr. 29 to May 23 in the year-normals of 1960s and it varied from Apr. 24 to May 16 in the normals of 2000s. The difference of the first appearance date of $15^{\circ}C$ was 0~10 days earlier in the year-normals of 2000s than the 1960s. The last harvesting date was determined to be the last appearance date of mean air temperature $15^{\circ}C$. The difference in the last appearance date of $15^{\circ}C$ was 1 to 13 days later in the year-normals of 2000s than in 1960s. The plant height of a rice variety, Hwayoung-byeo was 101~109 cm in 4 local areas, Seoul, Kangneung, Kwangju and Daegu. The plant height became 1~4 cm taller under warm condition. Rice grain yields estimated with daily weather data for the year-normals of 1960s and 2000s were 453~580 kg $10a^{-1}$ and 409~484 kg $10a^{-1}$ respectively. Rice grain yield of the former period was 50~100 kg $10a^{-1}$ higher than that hat in the later period.