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Effects of Single or Mixed Supplementation of Bacterial Phytase and Fungal Phytase on Laying Performance and Nutrient Digestibility (Bacterial 및 Fungal Phytase의 단일 및 혼합 급여가 산란계의 생산성과 영양소 이용에 미치는 영향)

  • Kang, H.K.;Park, S.Y.;Yu, D.J.;Kim, J.H.;Kang, G.H.;Na, J.C.;Kim, D.W.;Suh, O.S.;Lee, S.J.;Lee, W.J.;Kim, S.H.
    • Korean Journal of Poultry Science
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    • v.35 no.2
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    • pp.137-142
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    • 2008
  • This study was conducted to investigate the effect of single or mixed supplementation of bacterial and fungal phytase using 45-wk-old 450 Hy-Line Brown laying hens housed in individual cages for 12-wk period. The birds were reallocated to have similar egg productivity by examining the egg production for one wk before starting the experiment. Two sources of phytase, bacterial (BP) and fungal (FP), were used either in single or mixture to determine the effects of these phytase. Five dietary treatments consisted of control (BP 0, FP 0), T1 (BP 300, FP 0), T2 (BP 300, FP 300), T3 (BP 300, FP 3000), and T4 (BP 0, FP 3000). The DPU was used for phytase activity in this experiment. The nonphytate phosphorus (NPP) content of control was 0.30%, and those of phytase treatments were set to 60% of the Control. Experimental diets were fed ad libitum throughout the experimental period. The lighting schedule of 17L7D was employed. The egg production was not different between control and bacterial phytase treatments, but the T4 showed significantly low productivity compared to control (P<0.05). No difference was found in average egg weight among all treatments. The daily egg mass did not show any statistical differences among all treatments: however, it was significantly low in T4 compared to ther control during the latter half of the experiment (P<0.05). No significant difference was found among treatments in terms of feed intake, feed conversion and egg quality. The digestibilities of dry matter, crude protein, and fat digestibility were similar regardless of the treatments. No significant trends were detected in Ca and P availability. In conclusion, the BP level of 300 DPU contributed to achieve 40% reduction of recommended nonphytate phosphorus addition. The synergistic effect of bacterial and fungal phytase was not confirmed.

Some hair mineral contents of non-violent criminal and normal control (건강인(健康人)과 비폭력(非暴力) 범죄자(犯罪者)의 두발(頭髮)중 일부 금속원소(金屬元素) 함량(含量))

  • Hong, Sung-Cheul;Kim, Doo-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.26 no.1 s.41
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    • pp.110-125
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    • 1993
  • This study was designed to determine whether non-violent criminal and normal control on the basis of concentration of levels of trace mineral and toxic metal by analysis of human scalp hair. The subjects were selected 87 nonviolent criminal from a prison population and 120 normal control from periodic health checks for study. Hair samples were taken from the napes and Minnesota Multiple Personality Inventory (MMPI) was performed also. Five trace mineral (Zn, Cu, Mg, Fe, Na) and two toxic metal (lead, cadmium) contents were determined by an atomic absorption spectrometer. The contents of zinc and magnesium in hair of non-violent criminal were significantly lower than the control group (p<0.01). In the case of lead and cadimum, mean value of criminal group was significantly higher than control group. Significantly higher T-score of MMPI was seen in non-violent criminal group fur psychopathic deviate (Pd), paranoia scale (Pa), and Mania scale (Ma) than control group, but T-score of depression scale (D) was significantly higher in the control group. In the non-violent criminal group, the content of copper inversely proportion to T-score of Hs, D, Hy, Pd, Mf, Pa, Pt, Sc, Si except Ma, also Zinc inversely proportion to T-score of Hy, Mf, Pa, Pt. These results suggest that difference of some hair mineral contents exist between criminal and normal control group. Thus further studies are necessary to determine whether violent and nonviolent criminal group attributed biochemical imbalance with carefully constructed and controlled studies.

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Relationship between Violent Criminal Behavior and Imbalance of Scalp Hair Minerals in Man (두발중 미량 중금속과 필수금속의 과다 또는 과소의 불균형과 폭력범죄 행동과의 관련성 연구)

  • Kim, Doo-Hie;Jang, Bon-Ki;Lee, Duk-Hee;Hong, Sung-Chul;Kim, Byung-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.27 no.1 s.45
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    • pp.25-43
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    • 1994
  • To estimate the factors to the inclination of the criminal violence, the content of trace minerals and toxic metals in the scalp hair were measured during the period from May 1992 to October 1992. One hundred eleven violent and 89 nonviolent criminal inmates of Taegu Correctional Institute were selected. The inmates of violent criminals were imprisoned by murder, robber, rape, injury and violent acts. Those of nonviolent criminals were swindle, larceny, and adultery and had no history of institutional violence. The contents of two toxic metals (cadmium, lead) and five trace minerals (Cu, Fe, Zn, Mg, Na) were determined by an atomic absorption spectrophotometer (IL. 551). The contents of cadmium and lead in hair of violent criminals were significantly higher as $0.56{\pm}0.14ppm,\;11.53{\pm}3.32ppm$, respectively, than $0.42{\pm}0.20ppm,\;9.63{\pm}4.31ppm$ of nonviolent group (p<0.01). But the level of copper was significantly lower than nonviolent group (p<0.05). The factors that had a significant correlation with the inclination of violence in multiple logistic regression analysis were cadmium (odds ratio=98.09), unmarried (odds ratio=0.39), many times of criminal history(odds ratio=1.57) and residence of rural area (odds ratio=0.44). The results suggest that the sub-toxic contents of cadmium and lead in the hair may be of potential effect on behavior, and the mineral analysis may be an important adjunctive diagnostic procedure. Further studies into this problem are necessary.

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Changes in Quality of Muskmelon (Cucumis melo L.) during Storage at Different Temperatures (머스크멜론의 저장온도별 저장 중 품질변화)

  • Youn, Aye-Ree;Kwon, Ki-Hyun;Kim, Byeong-Sam;Kim, Sang-Hee;Noh, Bong-Soo;Cha, Hwan-Soo
    • Korean Journal of Food Science and Technology
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    • v.41 no.3
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    • pp.251-257
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    • 2009
  • The effects of temperature variations during storage on the quality characteristics of muskmelons (Cucumis melo L.) were investigated. In samples stored at $4^{\circ}C$ and $10^{\circ}C$, weight losses were almost 2.9- and 3.4-fold higher, respectively, compared to samples stored at $0^{\circ}C$. Soluble solids slightly increased except in the samples stored at $10^{\circ}C$, but acidity decreased over the entire storage period. Firmness decreased with storage time, but the samples stored at $0^{\circ}C$ had a lesser decrease in firmness than the samples stored at other temperatures. Water loss from the muskmelon stalk was most inhibited, and vitamin C content was maintained for the longest period, with storage at $4^{\circ}C$. Mineral contents (Ca, Na, Fe, Mg, K) were best maintained in muskmelon samples stored at $10^{\circ}C$ for 15 days, but levels had decreased by 30 days. Microbiological quality was not appreciably different at any storage temperature at 18 days; however, samples stored at $4^{\circ}C$ and $10^{\circ}C$ had deteriorated by 25 days. The results of sensory evaluations indicated that taste was best retained in samples stored at $10^{\circ}C$ for 15 days, although changes in taste were evident at all storage temperatures. When the samples were stored over 22 days at $10^{\circ}C$, retention of texture and overall acceptability were more inferior compared to samples stored at $0^{\circ}C$ and $4^{\circ}C$. These results suggest that storage at $4^{\circ}C$ can be used to reduce deterioration in muskmelons without significant loss of their quality attributes.

Comparison of Antioxidant, Cytotoxicity and Flavonoid Content of Stachys sieboldii Miq. vs. Lycopus lucidus Turcz. Leaf Extracts (초석잠 및 택란 잎 추출물의 항산화 및 세포독성 활성과 총 플라보노이드 함량 비교)

  • Na, Eun;Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.30 no.2
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    • pp.147-155
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    • 2020
  • In this study, the antioxidant and cytotoxic effects and the flavonoid contents of leaf extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. were compared. The flavonoid contents of the acetone + methylene chloride (A+M) and methanol (MeOH) extracts of L. lucidus Turcz. leaves were 55.7 and 233.2 mg/g, respectively. In a DPPH assay, A+M and MeOH extracts from L. lucidus Turcz leaves had a greater scavenging effect than those of S. sieboldii Miq. leaves (p<0.05). In an ABTS assay, MeOH extracts from S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) leaves had scavenging effects of 85% and 91%, respectively (p<0.05), suggesting that both of the MeOH extracts had greater scavenging effects than both A+M extracts. In a 120 min ROS production assay, all tested extracts decreased the cellular ROS production induced by H2O2 compared to that produced by exposure to the extract-free control. The MeOH extract from L. lucidus Turcz leaves had a greater inhibitory effect on cellular ROS production (p<0.05). Treatment with A+M and MeOH extracts from both S. sieboldii Miq. and L. lucidus Turcz. leaves showed a dose-dependent increased cytotoxicity against the growth of AGS, HT-29 cancer cells, and HT-1080 (p<0.05). Both A+M extracts had a greater inhibitory effect on the growth of all cancer cells than both MeOH extracts. These results suggest that the MeOH extract of L. lucidus Turcz. leaves is effective in scavenging free radicals and inhibiting cellular oxidation, while the A+M extract inhibits proliferation of three types of cancer cell.

The Quality Characteristics and Antioxidant Activity of Extracts of Schisandra chinensis Baillon Salad Dressing Prepared with Yam Juice and Mulberry (생마즙과 오디가 첨가된 오미자청 샐러드드레싱의 이화학적 특성 및 항산화성)

  • Kim, Hyung Don;Yim, Su Bin;Oh, Hye Lim;Jeon, Hye Lyun;Kim, Cho Rong;Kim, Na Yeon;Hong, Yoon Pyo;Lee, Ji Hyun;Kim, Mee Ree
    • Korean journal of food and cookery science
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    • v.28 no.5
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    • pp.531-540
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    • 2012
  • In this study, the effect of yam juice on the quality characteristics of the salad dressing was evaluated. Salad dressings were prepared with the salad dressing base (plain yogurt:mayonnaise:fresh cream=8:1:1), mulberry, omija for acidity, and yam for viscosity in the ratios of 4:4:6:1 and 4:4:6:3. Fresh yam juice was added at the level of 0, 7, and 18% of the salad dressing. As the concentration of the yam juice increased, the salad dressing increased in pH and decreased in acidity. The Hunter color L (lightness), b (yellowness) and a (redness) values of the salad dressing decreased as the amount of yam juice increased. The viscosity increased with the amount of yam juice. The total phenol content also increased with the amount of added yam juice. The antioxidant activities such as DPPH and hydroxyl radical activity of the mulberry salad dressing increased as the amount of yam juice was increased. The sensory preference test results showed that the salad dressings with added yam juice had higher scores in the color, flavor, taste and texture in comparison to the control. In the overall preference, the 7% yam juice added salad dressing had the highest score among the treatments. From these results, it was suggested that the salad dressing with the added mulberry, omija for acidity, and yam juice for viscosity was a functional salad dressing with high antioxidant activity.

Studies on the Petroleum hydrocarbon-utilizing Micro-organisms(Part 2) - On the Production of Single Cell Protein from Petroleum hydrocarbon with a yeast strain - (석유 탄화수소 이용 미생물에 관한 연구 (제 2 보) - 효모를 이용한 석유탄화수소로 부터 단백질 생산에 관하여 -)

  • Lee, Ke-Ho;Shin, Hyun-Kyung
    • Applied Biological Chemistry
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    • v.14 no.1
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    • pp.9-18
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    • 1971
  • In order to obtain basic information on the production of single cell protein from petroleum, more than 400 yeast strains were isolated from various soil samples in Korea utilizing petroleum hydrocarbon as the sole carbon source. A yeast strain showing the highest cell yield among the isolated strains was selected and identified. The optimal culture condition was searched in the flasks shaken throughout the procedure. And the growing characteristics for the selected yeast strain and chemical analysis of the yeast cell component were carried out. The results obtained were as follows: 1. The selected yeast strain was identified as Candida curvata and we named it Candida curvata-SNU 70. 2. The composition of the medium proposed for the present yeast strain is: Light Gas Oil 30ml, Urea 400mg, Ammonium sulfate 100mg, Potasium phosphate (monobasic) 670mg, Sodium phosphate (dibasic) 330mg, Magnesium sulfate 500mg, Calcium carbonate 3g, Yeast extract 50mg, Tween 20 0.05ml, Tap water 1,000ml. 3. Other culture conditions employed for the yeast were pH 5.5-7.0, temp. $30^{\circ}C$ under an affluent aerobic state. 4. Addition of light gas oil in portions to the culture media as the growth proceeded was more effective, especially in the cultivation on the higher oil concentration media. 5. Studies on the propagation of the yeast cells in the light gas oil medium revealed that the yeast has the lag phase lasted 16 hours and the logarithmic growth phase covered 16 to 28 hours. The specific growth rate was about $0.22\;hr^{-1}$ and doubling time was 3.2 hrs. during the logarithmic growth phase. 6. Under the cultural condition employed, the cell yield against the amount of light gas oil (wt%) was 16.1% and the protein content of the dried yeast cells was 48.4%.

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Investigation of Soil Contamination of Some Major Roadsides in Seoul -II. Major Roadsides in Gangdong-, Gwangjin-, Nowon-, Seodaemun- and Seongdong-gu- (서울시 주요 도로변 토양오염 조사 -II.강동구, 광진구, 노원구, 서대문구, 성동구 내 주요 도로변 토양-)

  • Kim, Kwon-Rae;Lee, Hyun-Haeng;Jung, Chang-Wook;Kang, Ji-Young;Park, Soon-Nam;Kim, Kye-Hoon
    • Applied Biological Chemistry
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    • v.45 no.2
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    • pp.92-96
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    • 2002
  • Present contamination levels of soils along the major roadsides of Seoul, Korea were investigated, and base-line data were accumulated for future use. Topsoil $(1{\sim}5$ cm) and subsoil $(20{\sim}50$ cm) from five districts (Gangdong-, Gwangjin-, Nowon-, Seodaemun- and Seongdong-gu) were sampled. The collected samples were airdried, passed through 2-mm sieves, and analyzed to determine the physicochemical properties including pH, EC, CEC, exchangeable cations (Ca, Mg, K, and Na), and heavy metal contents (Cd, Cu, Pb, and Zn). Soil textures of topsoils and subsoils were mainly loamy sand and sandy loam, respectively. The range of pH was $4.5{\sim}10$.0 with an average of 7.5 for both topsoil and subsoil, which is much higher than that of the forest soils in Seoul. The ranges of 0.1 N HCI extractable Cd, Cu, Pb, and Zn contents for both topsoils and subsoils were $0.0l{\sim}l.19$, N.D. (not $detected){\sim}228$.99, $N.D.{\sim}352$.54, and $2.97{\sim}332$.96 mg $kg^{-1}$, respectively. Most of the average heavy metal contents were lower than the concern level of the Soil Environment Conservation Act of Korea, but were much higher than those of the , forest soils in Seoul. Some sites were higher in heavy metal contents than the concern levels; in particular, the average Cu content in Seongdong-gu was much higher than the concern level, 50 mg $kg^{-1}$. Careful management of the soil to prevent the aggravation of the present contamination level and the dissemination of contamination is highly recommended.

Variation of Growth Characteristics and Quality Related Components in Korean Indigenous Tea (Camellia sinensis) Germplasms (한국 재래종 차나무(Camellia sinensis)의 작물학적 특성 및 품질관련 성분 변이)

  • Lee, Min-Seuk;Lee, Jin-Ho;Lee, Jeong-Dae;Hyun, Jin-Wuk;Kim, Young-Gul;Hwang, Young-Sun;Lee, Hyeon-Jin;Choi, Su-San-Na;Lee, Su-Jin;Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.333-338
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    • 2008
  • The tea has traditionally been used as a foodstuff by unique flavor, however recently not only the diversity of consumer demands but also the public interest in unique favorite and functional aspects have increased. It has been also reported that the main components contained in the leaves of tea (Camellia sinensis) include total nitrogen, free amino acids, polyphenols, and fiber, of which catechin has powerful bioactive effect such as anti-cancer, anti-aging, and anti-diabetic. (-)-Epigallocatechin gallate (EGCG) which is a major phenolic constituent of green tea extract has received considerable attention for a variety of important bioactivities. This study was carried out to obtain useful information for tea breeding programs, and to investigate the concentration of quality and functional related components in Korean indigenous tea germplasms. Korean indigenous tea lines were classified into three groups of sprout time, i.e, early, medium and late sprout time, and the ratio were 20%, 43% and 37%, respectively. There was a difference in characteristics among these Korean indigenous tea lines, leaf width of those ranged from 19.8 to 75 mm, leaf length was 35.5-160.0 mm, and leaf area was $660-8,400\;mm^2$. Experimental data on chlorophyll content (SPAD value) of Korean indigenous tea genetic resources ranged from 51.3 to 82.3. The concentrations of the total nitrogen, total free amino acids, and theanine were ranged 4.18-6.07%, 2.87-4.58%, and 1.64-2.66%, respectively. Also, catechin concentration showed from 11.54 to 15.07%, and concentration of caffeine was 2.82-4.23%. These results indicated indicated that it is possible to select elite lines with high concentration of quality related components and low concentration of caffeine from Korean domestic tea germplasms.

An Analysis and Evaluation of Current Cyber Home Learning Contents - Focused on the Earth Science Area of Science Course for the 10th Grade- (현행 사이버가정학습 콘텐츠의 분석 및 평가 -고등학교 1학년 과학과정의 지구과학 영역을 중심으로-)

  • Na, Jae-Joon
    • Journal of Science Education
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    • v.34 no.2
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    • pp.225-236
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    • 2010
  • The purpose of this study is to analyze and evaluate the Cyber Home Learning contents of Earth science area in the basic course of the $10^{th}$ grade. For this purpose, we applied the 'Cyber Home Study Content Quality Control Tool' presented in Elementary Secondary Education e-Learning Quality Management Guidelines (Ver.2.0)' of Korea Education & Research Information Service(2008). The results of Cyber home learning contents analysis are as follow: First, it was presented that the study guide introduced the contents which should be studied for one class, properly. And it was not analyzed that the diagnosis assesment was not completed in the initiative study; Second, it was possible to study choosing the contents fitting the learner's level of learning in the main study, it was comprised of about 10 minutes. Third, it was performed without feedback for incorrect answers in the learning assessment, just the number of wrong questions. And the learning arrangement present the important contents learned in that class, summarizing and arranging again. The results of evaluating the contents in Cyber Home Learning are as follows: First, in evaluation section of instructional design, many text materials which were so difficult for learners to read were explained, being provided. Besides, the systematic structures leaves much to be desired, in view of learners' learning experience, contents, and environment. And in evaluation section of learning contents, the error of contents caused the learning contents not to appear, the amount of learning in each section was found too much. Second, in evaluation section of the strategy for Teaching and Learning, when we mention the strategy of Self Directed Learning, the environment to make learners search for information free and self-study possible was not possessed well. And in evaluation section of interaction, it was found that a simple click caused the learning to go on. Third, in evaluation section of evaluating, it was evaluated that there was wanting in consistency in learning aims, contents, evaluation contents.

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