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Investigation of the Lactic Acid Bacteria Content of Probiotic and Lactic Acid Bacteria Products: a Study on Changes in the Preservation Method of Probiotic Products (프로바이오틱스와 유산균 제품의 균수 함량과 보존방법에 따른 변화 연구)

  • Kim, Young-Su;Hwang, Sun-Il;Kim, Sang-Tae;Han, Na-Eun;Kim, Hye-Young;Kim, Hyun-Soo;Park, Kwang-Hee;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.33 no.6
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    • pp.474-482
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    • 2018
  • The aim of this study was to determine and analyze probiotic contents, pH, and acidity of 120 samples of health functional and processed foods containing lactic acid bacteria distributed in 2017. The changes due to the preservation methods were also determined and analyzed in five probiotic products. Two samples of the 85 health functional food products had lactic acid bacteria at lower levels than the marked amount required to meet Korean food standards, whereas the 35 processed products were all suitable for distribution. The averages for probiotic contents, pH, and acidity were $1.2{\times}10^{10}CFU/g$, 5.35, and 1.29%, respectively. The average count of lactic acid bacteria in the 17 samples with marked amount among the 35 processed foods was $5.8{\times}10^8CFU/g$. The effects of preservation temperature ($-20^{\circ}C$, $4^{\circ}C$, $20^{\circ}C$, and $40^{\circ}C$) and storage period (1, 3, and 6 months) on probiotic content, pH, and acidity were determined for 5 probiotic products. After 1 to 6 months, the average reduction in probiotic content was by 59%; the lowest reduction occurred at $4^{\circ}C$ and the highest reduction occurred at $40^{\circ}C$. In addition, 3 of the 5 products showed a rapid decrease in probiotic content by more than 70% at $40^{\circ}C$ after a storage period of 1 to 3 months. Therefore, from this study results, it is recommended that products containing lactic acid bacteria should be refrigerated and consumed shortly after purchase.

Study on the Lubricity Characteristics of Bio-heavy Oil for Power Generation by Various feedstocks (다양한 원료에 따른 발전용 바이오중유의 윤활 특성 연구)

  • Kim, Jae-Kon;Jang, Eun-Jung;Jeon, Cheol-Hwan;Hwang, In-Ha;Na, Byung-Ki
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.985-994
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    • 2018
  • Bio-heavy oil for power generation is a product made by mixing animal fat, vegetable oil and fatty acid methyl ester or its residues and is being used as steam heavy fuel(B-C) for power generation in Korea. However, if the fuel supply system of the fuel pump, the flow pump, the injector, etc., which is transferred to the boiler of the generator due to the composition of the raw material of the bio-heavy oi, causes abrasive wear, it can cause serious damage. Therefore, this study evaluates the fuel characteristics and lubricity properties of various raw materials of bio-heavy oil for power generation, and suggests fuel composition of biofuel for power generation to reduce frictional wear of generator. The average value of lubricity (HFRR abrasion) for bio-heavy oil feedstocks for power generation is $137{\mu}m$, and it varies from $60{\mu}m$ to $214{\mu}m$ depending on the raw materials. The order of lubricity is Oleo pitch> BD pitch> CNSL> Animal fat> RBDPO> PAO> Dark oil> Food waste oil. The average lubricity for the five bio-heavy oil samples is $151{\mu}m$ and the distribution is $101{\mu}m$ to $185{\mu}m$. The order of lubricity is Fuel 1> Fuel 3> Fuel 4> Fuel 2> Fuel 5. Bio-heavy oil samples (average $151{\mu}m$) show lower lubricity than heavy oil C ($128{\mu}m$). It is believed that bio-heavy oil for power generation is composed of fatty acid material, which is lower in paraffin and aromatics content than heavy oil(B-C) and has a low viscosity and high acid value, resulting in inhibition of the formation of lubricating film by acidic component. Therefore, in order to reduce friction and abrasion, it is expected to increase the lubrication of fuel when it contains more than 60% Oleo pitch and BD pitch as raw materials of bio-heavy oil for power generation.

Investigation of Unintentionally Hazardous Substance in Commercial Herbs for Food and Medicine (유통 식약공용농산물 중 비의도적 유해물질 오염도 조사)

  • Seo, Mi-Young;Kim, Myung-Gil;Kim, Jae-Kwan;Jang, Mi-Kyung;Lee, Yu-Na;Ku, Eun-Jung;Park, Kwang-Hee;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.33 no.6
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    • pp.453-459
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    • 2018
  • This study was performed to investigate the contamination levels of heavy metals (such as lead, cadmium, arsenic and mercury) and aflatoxin (such as $B_1$, $B_2$, $G_1$ and $G_2$) in commercial herbs for food and medicine. The concentrations of the heavy metals were measured by the ICP-MS and a mercury analyzer. The aflatoxins were analyzed by a HPLC-florescence coupled with photochemical derivatization. The detection ranges of the lead, cadmium, arsenic and mercury were found to be 0.006~4.088 mg/kg, 0.002~2.150 mg/kg, ND~0.610 mg/kg and ND~0.0139 mg/kg respectively. Among the total samples, the 3 samples (2.6%) were not suitable for the specification of cadmium by the MFDS (Ministry of Food and Drug Safety). The 13 samples of the total 117 samples were aflatoxin positive (11.1%). The amount of aflatoxin $G_1$ was $0.7834{\mu}g/kg$ in the Puerariae Radix and aflatoxin $G_2$ were $0.3517{\mu}g/kg$, $0.4881{\mu}g/kg$ in two samples of the Glycyrrhizae Radix et Rhizoma, respectively. The aflatoxins $B_2$ and $G_1$ were simultaneously detected in the 10 Angelicae Gigantis Radix. The detection ranges of aflatoxins $B_2$ and $G_1$ were $0.2324{\sim}1.0358{\mu}g/kg$ and $0.7552{\sim}1.6545{\mu}g/kg$ respectively in Angelicae Gigantis Radix. The results of the current study suggest that continuous monitoring is needed for the proactive management of commercial herbs for food and medicine safety.

Organic Cucumber Productivity Affected by Long-term Application with Homemade Liquid Fertilizers (자가제조액비 장기연용 처리가 유기 오이의 생산성에 미치는 영향)

  • Choi, Hyun-Sug;Jung, Ji-Sik;Jung, Seok-Kyu
    • Korean Journal of Organic Agriculture
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    • v.27 no.1
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    • pp.87-100
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    • 2019
  • The study was initiated to compare crop productivity as affected by a long-term application with homemade liquid fertilizers in leading organic cucumber (Cucumis sativus L.) farms in Suncheon and Kimcheon provinces. A Suncheon farm have applied an EM (effective organism) liquid fertilizer for one year and fifteen years, designating as EM 1-year and EM 15-year plots, respectively, with 4-year and 5-year application of native microbes-liquid fertilizer in Kimcheon farm, designating as Micro 4-year and Micro 5-year plots, respectively. pH in the EM-liquid fertilizer was high to approximately 7.7, and EC in the Micro-liquid fertilizer was 0.1 dS/m higher than those of EM-liquid fertilizer, with similar macro-nutrient concentrations observed in the both liquid fertilizers. Soil EC was the highest to the 10.0 dS/m for the liquid fertilizer with EM 1-year and showed less than 1.5 dS/m for other liquid fertilizer plots. Micro-liquid fertilizer plots had soil OM contents less than 20 g/kg, which was approximately two times less than those of EM plots. Soil microbial properties were not significantly different among the liquid fertilizer plots. SPAD and PS II values were significantly increased by EM 15-year plots with high levels of soil OM and EC. Liquid fertilizer plot with EM 1-year had high concentrations of T-N, Ca, and Na in the cucumber crops but low concentrations of P and Mg, in particular for low K of 1.2% which was two times less than those of desired level for an optimum cucumber growth. The lowest fruit yield was observed for the liquid fertilizer plot with EM 1-year with the highest soil EC accumulated. Liquid fertilizer plot with EM 15-year produced the expanded volume of crop canopy and increased fruit yield. Therefore, long-term of continuous application with an organic liquid fertilizer would have sustainably improved soil stability and the crop productivity.

Classification and identification of organic aerosols in the atmosphere over Seoul using two dimensional gas chromatography-time of flight mass spectrometry (GC×GC/TOF-MS) data (GC×GC/TOF-MS를 이용한 서울 대기 중 유기 에어로졸의 분류 및 동정)

  • Jeon, So Hyeon;Lim, Hyung Bae;Choi, Na Rae;Lee, Ji Yi;Ahn, Yun Kyong;Kim, Yong Pyo
    • Particle and aerosol research
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    • v.14 no.4
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    • pp.153-169
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    • 2018
  • To identify a variety of organic compounds in the ambient aerosols, the two-dimensional gas chromatography-time of flight mass spectrometry (GCxGC) system (2DGC) has been applied. While 2DGC provides more peaks, the amount of the generated data becomes huge. A two-step approach has been proposed to efficiently interpret the organic aerosol analysis data. The two-dimensional 2DGC data were divided into 6 chemical groups depending on their volatility and polarity. Using these classification standards, all the peaks were subject to both qualitative and quantitative analyses and then classified into 8 classes. The aerosol samples collected in Seoul in summer 2013 and winter 2014 were used as the test case. It was found that some chemical classes such as furanone showed seasonal variation in the high polarity-volatile organic compounds (HP-VOC) group. Also, for some chemical classes, qualitative and quantitative analyses showed different trends. Limitations of the proposed method are discussed.

Epidemiological investigation on the outbreak of foodborne and waterborne disease due to Norovirus with delayed notification (노로바이러스에 기인한 수인성·식품매개감염병 집단발생의 지연신고에 대한 역학조사)

  • Ha, Mikyung;Kim, Hyeongsu;Kim, Yong Ho;Na, Min Sun;Yu, Mi Jung
    • Journal of agricultural medicine and community health
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    • v.43 no.4
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    • pp.258-269
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    • 2018
  • Objectives: There was an outbreak of foodborne and waterborne disease among high school students at Okcheon in June, 2018. First attack occurred June $5^{th}$ but seven days later it was notified. The purpose of this investigation was to evaluate the pathogen of outbreak and cause of delayed notification. Methods: First, we did a questionnaire survey for 61 cases and 122 controls to find what symptoms they had and whether they ate foods or drank water from June $2^{nd}$ to June $12^{th}$. Second, we investigated the environment of cafeteria and drinking water. Third, we examined specimen of cases and environment to identify bacteria or virus. Results: Attack rate of this outbreak was 7.8%. Drinking water was strongly suspected as a source of infection in questionnaire survey but we could not find the exact time of exposure. Norovirus was identified in specimen of cases (2 students), drinking water (at main building and dormitory) and cafeteria (knife, dishtowel, hand of chef) Conclusions: We decided norovirus as the pathogen of this outbreak based on the clinical features of cases with diarrhea vomiting, abdominal pain and recovery within 2 or 3 days after onset, outbreak due to drinking water and microbiologic examination, And the cause of delayed notification might be the non-existence of the nurse teacher at that time and the lack of understanding of teachers on immediate notification under the outbreak. To prevent the delayed notification, notification system about outbreak of foodborne and waterborne disease in school is needed to be improved.

A Comparative Analysis of Korean and Chinese Medicinal Plant Resources and Traditional Knowledge Using Data Analysis (데이터 분석기법을 이용한 한국과 중국의 약용식물자원과 전통지식 정보 비교분석)

  • Na, Minho;Hong, Seong-Eun;Kim, Ki-Yoon;Cheong, Eun Ju
    • Journal of Korean Society of Forest Science
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    • v.107 no.4
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    • pp.456-477
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    • 2018
  • We analyzed the data on medicinal plants and related traditional knowledge in Korea and China using data analysis method. There are 108 families 214 genera 542 species, and 202 families 660 genera 1,261 species were found in Korea and China respectively. Total of 86 families (79.6%) and 130 genera (60.7%) were in common both countries. More than one information were recorded in many species, however, there was only one information from some species (32.7% of genera in Korea, and 58.8% of genera in China). The most frequent appeared plant family was Compositae (Asteraceae) in both countries (8.4% in Korea and 10.7% in China) and followed by Rosecease and Leguminocae (Fabaceae). Plant parts were classified 11 parts and roots were mostly used in Korea and whole plants in China. Usages were described in different terms of ailments or symptoms. There are 120 usage in Korea and 230 in China. Plant uses for the ailment or symptoms are pain, digestive system disorder, cold and etc. in Korea. In China, plants were mostly used for clear heat, digestive system disorder, cough and etc. Relation between the plant and ailment(symptom) of the top 10 plants in Korea and China was different although from same plant family. We also analyzed the relations between plant species and part used, and plants parts and ailment(symptom). With the data analysis method, we were able to collect the medicinal plant resources data and found the differences in plant resources, usage, and plant part for use. The result provide important information of the plant resources and related traditional knowledge of Korea for use of plant resources in industry and facilitate to plan a strategy to cope with Nagoya Protocol in the future.

Antioxidant Effect of Extracts from 9 Species of Forest Plants in Korea (국내 9종 산림식물 추출물의 항산화 효능)

  • Sim, Wan-Sup;Lee, Jong Seok;Lee, Sarah;Choi, Sun-Il;Cho, Bong-Yeon;Choi, Seung-Hyun;Han, Xionggao;Jang, Gill-Woong;Kwon, Hee-Yeon;Choi, Ye-Eun;Kim, Jong-Yea;Kim, Jong-Dai;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.34 no.4
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    • pp.404-411
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    • 2019
  • This study was carried out to investigate the antioxidant effects of extracts from 9 species of forest plants in Korea. DPPH, ABTS, $NaNO_2$, hydrogen peroxide radical scavenging activity and reducing power activity were evaluated to measure the antioxidant activities of plant extracts. As a result, Geranium thunbergii has been identified as the most effective antioxidant resource. Also, total phenolic content was highest in Geranium thunbergii ($303.94{\pm}0.63mg\;GAE/g$) among 9 species extracts. Total flavonoid content was highest in Rosa multiflora ($24.32{\pm}0.22mg\;QE/g$) and proanthocyanidin content was highest in Vitis ficifolia ($279.00{\pm}4.58mg\;CE/g$) among 9 species extracts. In addition, the protective effect of plant extracts in $H_2O_2-induced$ human dermal fibroblast (HDF) cell systems were also assessed. Significant protective effects in $H_2O_2-induced$ human dermal fibroblast (HDF) cell systems were found in all plant extracts, especially in Geranium thunbergii. These results suggest that Geranium thunbergii could be a potential natural resource for antioxidant activity.

Assessment on Accuracy of Stereotactic Body Radiation therapy (SBRT) using VERO (VERO system을 이용한 정위적 체부 방사선치료(SBRT)의 정확성 평가)

  • Lee, Wi Yong;Kim, Hyun Jin;Yun, Na Ri;Hong, Hyo Ji;Kim, Hong Il;Baek, Seung Wan
    • The Journal of Korean Society for Radiation Therapy
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    • v.31 no.1
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    • pp.17-24
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    • 2019
  • Purpose: The present study aims to assess the level of coherency and the accuracy of Point dose of the Isocenter of VERO, a linear accelerator developed for the purpose of the Stereotactic Body Radiation Therapy(SBRT). Materials and Method: The study was conducted randomly with 10 treatment plans among SBRT patients in Kyungpook National University Chilgok Hospital, using VERO, a linear accelerator between June and December, 2018. In order to assess the equipment's power stability level, we measured the output constancy by using PTW-LinaCheck, an output detector. We also attempted to measure the level of accuracy of the equipment's Laser, kV(Kilo Voltage) imaging System, and MV(Mega Voltage) Beam by using Tofu Phantom(BrainLab, Germany) to assess the accuracy level of geometrical Isocenter. We conducted a comparative analysis to assess the accuracy level of the dose by using an acrylic Phantom($30{\times}30{\times}20cm$), a calibrated ion chamber CC-01(IBA Dosimetry), and an Electrometer(IBA, Dosimetry). Results: The output uniformity of VERO was calculated to be 0.66 %. As for geometrical Isocenter accuracy, we analyzed the error values of ball Isocenter of inner Phantom, and the results showed a maximum of 0.4 mm, a minimum of 0.0 mm, and an average of 0.28 mm on X-axis, and a maximum of -0.4 mm, a minimum of 0.0 mm, and an average of -0.24 mm on Y-axis. A comparison and evaluation of the treatment plan dose with the actual measured dose resulted in a maximum of 0.97 % and a minimum of 0.08 %. Conclusion: The equipment's average output dose was calculated to be 0.66 %, meeting the ${\pm}3%$ tolerance, which was considered as a much uniform fashion. As for the accuracy assessment of the geometric Isocenter, the results met the recommended criteria of ${\pm}1mm$ tolerance, affirming a high level of reproducibility of the patient's posture. The difference between the treatment plan dose and the actual measurement dose was calculated to be 0.52 % on average, significantly less than the 3 % tolerance, confirming that it obtained predicted does. The current study suggested that VERO equipment is suitable for SBRT, and would result in notable therapeutic effect.

Toxicity Assessment of Antifouling Agent using the Survival and Population Growth Rate of Marine Rotifer, Brachionus plicatilis (해산로티퍼(Brachionus plicatilis)의 생존 및 개체군 성장률을 이용한 신방오도료(Zinc undecylenate)의 독성평가)

  • Hwang, Un-Ki;Choi, Hoon;Park, Yun-Ho;Park, Na-Young;Jang, Soo-Jung;Lee, Seung Min;Choi, Yun-Seok;Yang, Joon-Yong;Lee, Ju-Wook
    • Korean Journal of Environmental Biology
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    • v.36 no.3
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    • pp.392-399
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    • 2018
  • Toxicity assessment of antifouling agent, zinc undecylenate (ZU) has been investigated using the rate of survival and population growth in marine rotifer, Brachionus plicatilis. The survival rate of Brachionus plicatilis was determined after 24 h of exposure to ZU and was not affected up to the maximum level of $100.0mg\;L^{-1}$ of ZU. The population growth rate (r) was determined after 72 h of exposure to ZU. It was observed that r in the controls (absence ZU) was greater than 0.5 but exhibited a sudden decrease with an increase in the concentration of ZU. ZU reduced r in a dose-dependent manner and a significant reduction occurred at a concentration greater than $12.5mg\;L^{-1}$. The 50% effective concentration ($EC_{50}$) value of r during ZU exposure was $26.4mg\;L^{-1}$, No-observed-effect-concentration (NOEC) was $6.3mg\;L^{-1}$ and Lowest-observed-effect-concentration (LOEC) was $12.5mg\;L^{-1}$, respectively. Based on the results, it is apparent that ZU concentration greater than $12.5mg\;L^{-1}$ exhibited a toxic effect on the r of zooplankton, B. plicatilis in natural ecosystems.