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Effects of Glucose and Inorganic Phosphate on the Development of Rat 8-Cell Embryos In Vitro (Glucose와 Inorganic Phosphate가 Rat 8-세포기 난자의 체외배양에 미치는 영향)

  • 이홍미;진동일
    • Korean Journal of Animal Reproduction
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    • v.20 no.3
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    • pp.251-258
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    • 1996
  • This study was designed to evaluate the potential inhibitory effects of glucose (5.56 mM vs. 0 mM) and/or phosphate (potassium phosphate, 1.19 mM vs. 0 mM) on the in vitro devel-opment of rat 8-cell embryos (n=345 embryos from 36 mature rats). Evaluation of embryos at 48 h for developmental stage (STG) indicated that 37% (31/84), 70% (64/91), 69% (59/85), and 77% (67/85) developed to the blastocyst stage in media with glucose+phosphate, glucose only, phosphate only, and no glucose or phosphate, respectively. Embryo development (2.90${\pm}$0.097 for STG) in medium with glucose + phosphate was significantly reduced (P<0.001), while no significant differences were observed between all other media (3.4~3.5${\pm}$0.093-0.097 for STG). Evaluation of embryos for final cell number (FCN) indicated that the greatest number of cells (nuclei) resulted in medium with glucose alone (29.3${\pm}$0.97 cells, P<0.001). No significant differences were observed for FCN for the remaining three media (l7.5${\pm}$1, 04 cells, 18.6${\pm}$1.Ol cells, and 19.8${\pm}$1.01 cells for glucose+phosphate, phosphate only, and no glucose or phosphate, respectively). Our results suggest that glucose and phosphate together exert an inhibitory effect on 8-cell rat embryo development, while glucose alone was beneficial, yielding greater numbers of cells per embryo.

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Sex Determination in Somatic and Embryonic Cells of the Pig by FISH and PCR (FISH와 PCR에 의한 돼지 체세포 및 배아세포의 성 판정)

  • Chung, Y.;Jeon, J.T.;Kim, K.D.;Lee, S.H.;Hong, K.C.
    • Korean Journal of Animal Reproduction
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    • v.20 no.3
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    • pp.323-331
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    • 1996
  • Predetermination of sex in mammalian species has many aspects of application including the prenatal diagnoses of genetic disorders in humans and sex-selected breeding programs in the animal industry. Embryos sexing can be carried out using the polymerase chain reaction (PCR) to amplify specific sequences present in the sex chromosomes, or by fluorescent in situ hybridization (FISH) of specific probes to the X and Y chromosomes. A 3.3 kb porcine male-specific DNA fragment (pEM39) was cloned previously in our laboratory. In this study, FISH and PCR methods were employed to examine if the pEM39 can be used a sex-specific DNA probes Porcine ovaries were obtained from a local slaughter house and oocytes collected. All oocytes were subjected to in vitro maturation followed by 1n vitro fertilization. Parthenogenetically activated embryos were served as a negative control. Embryonic samples were collected at the 2-cell stages and PCR was performed to analyze DNA. Among 10 embryos examined, four embryos were identified as males and six were females. The cloned male-specific DNA fragment showed male-specificity for the cells in the liver tissue and the porcine early embryos by FISH. It was also demonstrated that the cloned male-specific DNA is localized on the hetero chromatic region of the long arm in the Y chrom-osome (Yq) as shown by the FISH and karyotyping. The results suggest that the cloned male-specific DNA fragment may be useful for predetermination of sex with a few embryonic cells. The porcine male-specific sequence can be a reliable index for embryo sexing by PCR.

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MPEG-21 Terminal (MPEG-21 터미널)

  • 손유미;박성준;김문철;김종남;박근수
    • Journal of Broadcast Engineering
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    • v.8 no.4
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    • pp.410-426
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    • 2003
  • MPEG-21 defines a digital item as an atomic unit lot creation, delivery and consumption in order to provide an integrated multimedia framework in networked environments. It is expected that MPEG-21 standardization makes it Possible for users to universally access user's preferred contents in their own way they want. In order to achieve this goal, MPEG-21 has standardized the specifications for the Digital Item Declaration (DID). Digital Identification (DII), Rights Expression Language (REL), Right Data Dictionary (RDD) and Digital Item Adaptation (DIA), and is standardizing the specifications for the Digital Item Processing (DIP), Persistent Association Technology (PAT) and Intellectual Property Management and Protection (IPMP) tot transparent and secured usage of multimedia. In this paper, we design an MPEG-21 terminal architecture based one the MPEG-21 standard with DID, DIA and DIP, and implement with the MPEG-21 terminal. We make a video summarization service scenario in order to validate ow proposed MPEG-21 terminal for the feasibility to of DID, DIA and DIP. Then we present a series of experimental results that digital items are processed as a specific form after adaptation fit for the characteristics of MPEG-21 terminal and are consumed with interoperability based on a PC and a PDA platform. It is believed that this paper has n important significance in the sense that we, for the first time, implement an MPEG-21 terminal which allows for a video summarization service application in an interoperable way for digital item adaptation and processing nth experimental results.

Development of 1.0 Tesla Compact MRI System (1.0 Tesla 자기 공명 진단 장치의 개발)

  • Lee, H.K.;Oh, C.H.;Ahn, C.B.;Chang, Y.H.;Shin, D.W.;Lee, K.N.;Jang, K.H.
    • Proceedings of the KOSOMBE Conference
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    • v.1996 no.11
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    • pp.129-134
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    • 1996
  • 1차 년도 G-7 개발 과제로 수행된 자기 공명 진단 장치 (Magnetic Resonance Imaging System)의 개발 내용을 간략히 소개하였다. 성공적인 IT Compact 자기 공명 진단 장치의 완성을 위해 일차적으로 (1)RF (고주파), Gradient(경사 자계), Spectrometer 등의 Hard-ware 관련 MRI 핵심부분, (2) RF, Gradient, Spectrometer, Magnet 등의 각 Sub-system을 연결, 조합, 조정하여 하나의 체계적인 시스템으로 통합하고 운영하는 과정(System Integration), (3)사용자와 시스템을 연결하는 User Interface, Data Base Management, Real time 운영 SW 등과 (4)임상에 적용하여 구체적인 성능과 효용성을 확인하는 기술 등에 대하여 집중 연구하였다. 개발 방법은 (1)지난 16년간 국내에 축적 된 연구 개발 인력들을 최대한 활용하고 (2)연구 개발을 국제화 시켜 필요한 경우 부분별로 개발 인력을 해외에서 보완하고 (3)소수 정예 전문 인력 주의와 요소 기술 또는 중요 부품을 경쟁성 검토 후 필요 시 Out-sourcing 활용으로 최저의 비용으로 개발 기간을 최소화 하는 데 두었다. 개발된 1.0Tesla자기 공명 영상 장치는 미국 물리 학회에서 규격화한 Phantom및 임상 적용을 통하여 서울대 의대 연구 팀과 지속적으로 성능을 평가해 왔다. 개발된 시스템의 해상도는 $256{\times}256$ head 영상에서 1mm 이 하의 해상도를 가짐을 resolution phantom 을 통하여 확인할 수 있었고, $512{\times}512$ 영상에서 는 약 0.5 mm 의 물체를 분리 해냄으로써 외제 시스템들 보다 우수하게 평가 되었다. 차폐 경사코일의 Eddy current영향은2%이내로 촬영 시 영향은 거의 무시할 수 있었다. 또한, 개발된 영상 기법들, 즉 Multislice/Multi Echo, Oblique angle imaging, 64 Echo train을 갖는 고속 촬영 기술들이 자기 공명 장치에 장착되어 임상 적용에 문제가 없도록 하였다. 또한 20mT/m/Amp의 강력한 능동 차폐 경사 자계 코일(Active Shield Gradient Coil)을 기본 사양으로 하고, 수신단을 최대 6개로 확장토록 하여 2차년도의 초고속 촬영 기법(EPI) 및 Phased Array 코일 촬영이 가능토록 하였다. 1차 년도 개발 과제 수행 결과와 향후 개발 과제를 바탕으로 최종 목표인 국제 경쟁력이 있는 자기 공명 진단 장치 즉 기능과 영상의 질은 선진국 제품과 동일하거나 우수하되, 저가격을 구현한 상용화 제품이 완성되어, 첨단 의료기기로서 산업 구조 고도화에 기여하고 수입대체 뿐만 아니 라 수출을 통한 국익 창출과 국가의 기술을 통한 위상 제고에 기여되길 기대한다.

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Investigation of Nonylphenols Contamination in Solvents and Solid-phase Extraction Cartridge, and its Removal Protocols (정밀분석용 용매 및 SPE의 Nonylphenols 오염평가 및 제거)

  • Park, Jong-Min;Choi, Geun-Hyonng;Kim, Jung-Im;Hong, Su-Myeong;Kwon, Oh-Kyung;Im, Geon-Jae;Kim, Jin-Hyo
    • The Korean Journal of Pesticide Science
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    • v.15 no.1
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    • pp.22-27
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    • 2011
  • Nonylphenols are toxic compounds classified as endocrine disruptors. We investigated the nonylphenols clean-up procedures for the contamination control in the quantitative analysis. In this research we analyzed the residual nonylphenols in the solvent and the SPE cartridges. First, at the analysis of HPLC grade solvents (n-hexane, diethyl ether, ethyl acetate and its mixture), diethyl ether was confirmed the residue as 0.963 ${\mu}g/mL$, and we eliminated the contaminant through the distillation with $CaH_2$, Second, at the analysis of SPE cartridges (silica gel and Florisil), all products were showed the residue at 0.046~13.0 ${\mu}g/mL$, but unfortunately the residue in the cartridge were not easily removed with referenced methods in all tested SPE cartridges except in silica gel SPE cartridge with glass ware.

A study on Fire Case and Countermeasure of Tourist Hotel (관광호텔의 화재손해 위험관리방안 - 화재발생현황과 대형화재사례 분석 중심)

  • Han, Sukman;Son, Jung Hyoun;Kim, Jong Won
    • Journal of the Society of Disaster Information
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    • v.8 no.4
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    • pp.362-375
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    • 2012
  • Tourist hotels are equipped with facilities such as accommodation and restaurants, exercise, recreation. Unspecified guests, visitors and management of tourist hotels are very vulnerable on the casualties and property losses due to fire peril exist. In this study, we analysis that the fire statistics status of tourist hotels from 2001 to 2010. And the 15 cases of a large hotel fire are reviewed. The total number of fires on hotel are consist of a hotel rooms fire(33.2%), a restaurant kitchen fire(11.8%). And the major causes of the fire are an electrical fire (40.8%), a cigarette fire (14.5%) and a hot-work fire (9.2%). In case study, the fire wall defect and combustible materials are major fire loss causes for 10year. Each tourist hotels are needed a development of suitable fire risk management and a field operations. A hotel is required an active fire risk management on a preventive inspection, an education and training, and a preventive maintenance. It is necessary that a fire wall maintenance to prevent of the spread of a fire and a sprinkler installation of whole area to protect fire. And it is very important an emergency response for evacuation of guest, and operate emergency procedures on a fire or emergency situation.

Effect of cassia cinnamon intake on improvement of the glycemic response: An updated meta-analysis - Focus on preparation of dehydrated powder and water extract (계피의 혈당 개선 기능성 평가 : 메타분석 - 건조분말과 물추출물을 중심으로)

  • Kwak, Jin Sook;Park, Min young;Kwon, Oran
    • Journal of Nutrition and Health
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    • v.50 no.5
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    • pp.437-446
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    • 2017
  • Purpose: This meta-analysis aimed to evaluate the effect of cassia cinnamon (Cinnamomum. cassia and C. aromaticum) on the glycemic response with a focus on the preparation of dehydrated powder and water extract. Methods: We searched Pubmed, Cochrane, EMBASE, Science Direct, and the Korean Studies Information Service System (KISS) through May 2017. In the meta-analysis for the preparation of powder, eight trials reporting fasting blood glucose (FBG), four trials reporting HbA1c, and three trials reporting the postprandial glycemic response were included. For the water extract, six trials reporting FBG and four trials reporting HbA1c were eligible for this study. A random-effects model was used to calculate the pooled effect size. Results: Cassia cinnamon powder intake significantly lowered FBG by -1.55 mmol/L (95% CI, -2.45, -0.64; p = 0.001) and the AUC of postprandial blood glucose level by $-51.8mmol/L{\cdot}min$ (95% CI, -85.5, -18.1; p = 0.003). There was a significant difference in FBG between water extract of cinnamon and placebo of -0.76 mmol/L (95% CI, -1.09, -0.43; p = 0.000). However, blood HbA1c level was not significantly altered by any preparation of cinnamon. No statistical heterogeneity was observed for any analysis except in the case of FBG for cinnamon powder. Results of funnel plots and Egger's regression suggest a low likelihood of publication bias in all biomarkers (p > 0.05). Conclusion: According to this meta-analysis, there was possible evidence to support a relationship between cassia cinnamon intake and fasting glucose in both preparation of powder and water extract. Furthermore, new evidence of the health benefits on postprandial glucose regulation of cinnamon powder was obtained.

Studies on the browning inhibition of yam(Dioscorea aimadoimo) during hot air dehydration (단마(Dioscorea aimadoimo)의 열풍건조 시 갈변 억제 방안 연구)

  • Chung, Yong-Yul;Jeong, Woo-Sik;Chung, Shin-Kyo
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.384-388
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    • 1996
  • This research was conducted to investigate the effective methods for browning inhibition on yam (Dioscorea aimadeimo) during dehydration by physical and chemical pretreatments. Moisture, crude protein, crude fiber and N-free extract contents of yam were 81.17%, 1.43%, 0.29% and 15.81%, respectively. Yams were sliced to 0.5 cm thickness and placed to single and poly layer in plastic tray, and then changes of their weights were measured during air dehydration at $50^{\circ}C,\;65^{\circ}C,\;and\;80^{\circ}C$. The dehydration time reaching to optimum moisture level for the pulverization of the yam slices were 10, 6, 3 hours(single layered) and 12, 7, 5 hours(multi layered) at the respective temperature. To inhibit browning at $80^{\circ}C$ air dehydration, water and steam blanching, microwave treatment effects were investigated on yam slices for 30 sec. and 60 sec. Steam blanching for 30 sec. was comparatively effective to inhibit browning of yam slices. Yam slices were immersed in single and combined browning inhibitor solutions and evaluated for browing degree during dehydration by the values of Hunter L, a, b and ${\Delta}E$. The most effective pretreatment to inhibit browning of yam slices was immersion In the solution containing 500 ppm of citric acid and 1000 ppm of cysteine for 1 min.

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Soil Condition and Vegetation Structure in Acer mono for. rubripes Stand in Geoje, Gyeongnam Province (경남 거제지역 붉은고로쇠나무림의 토양특성과 식생구조)

  • Moon Hyun Shik;Roh Il;Kim Jong Kab;Kwon SuDeok
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.6 no.4
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    • pp.265-271
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    • 2004
  • This study was carried out to furnish basic information on the habitat through soil condition and vegetation structure of an Acer mono for. rubripes stand in Geoje, Gyeongnam Province. Soil pH of the A. mono for. rubripes stand was 5.17. The contents of organic matter, total N and available P205 were 8.7%,0.38%, 15.7ppm, respectively. A. mono for. rubripes, Meliosma oldhamii, Zelkova serrata, Styrax japonica, Sapium japonicum, Lindera erythrocarpa and Euonymus oxyphyllus appeared in all layers. The importance values of A. mono for. rubripes, Z. serrata and Quercus serrata in the upper layer were high, S. japonicum and M. myriantha were high in the middle layer and lower layer, respectively. Species diversity and evenness ranged from 0.931 of the middle layer to 1.638 of the lower layer, and from 0.706 of the upper layer to 0.959 of the lower layer, respectively. The study results provide basic information on soil condition and vegetation structure of the Acer mono for. rubripes stand native to Geoje, Korea.

The Design and Implementation of Ontology for Simulation based Architecture Framework (ONT-AF) in Military Domain (SBA AF의 구축을 지원하는 온톨로지의 설계 및 구현(ONT-SAF))

  • Kwon, Youngmin;Sohn, Mye;Lee, Wookey
    • Journal of Information Technology and Architecture
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    • v.9 no.3
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    • pp.233-241
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    • 2012
  • Architecture framework (AF) is a guideline to define components needed to develop and operate enterprise architecture (EA), and to define relationships among the components. There are many architecture frameworks to operate EA of governments and businesses such as Zachman framework, DoDAF, TOGAF, FEAF, and TEAF. DoDAF is the most representative AF to support the development of the EA in the military domain. DoDAF is composed of eight viewpoints and 40 views that are affiliated with the viewpoints. To develop an AF for a specific goal, system architects decide a set of views. Furthermore, they determine data that are needed for a view modeling. However, views and data in DoDAF are structurally inter-related explicitly and/or implicitly. So, developing an AF for a specific goal is going to be a project to be carried out over a long haul. To reduce the burden of its development, in this paper, we develop ONT-SAF (Ontology for DoDAF) that can infer inter-relationships like referential and transitive relationships and the sequences among the views. Furthermore, to promote reusability and consistency of the views and the data within an AF, we adopt the view-data separation strategy. ONT-DAT contains classes like 'viewpoint', 'view', 'data', 'expression method', and 'reference model', and 11 properties including 'hasView.' To prove the effectiveness of ONT-SAF, we perform a case study.