• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1242-1248
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• 2005

This study was designed to investigate the in vitro developmental ability and apoptosis of bovine embryos nucleartransferred (NT) with frozen-thawed or cooled donor cells. Cultured adult bovine ear cells were used as donor cells after sub-culturing to confluence (CC), cooling to 4$^{\circ}C$ for 48 h, or freezing-thawing (FT). Apoptotic cells in blastocysts were evaluated for apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. Fusion, cleavage and blastocyst rates were 69.0 (167/242), 68.8 (115/167), and 29.9 (50/167) with CC cells, 70.4 (88/125), 69.3 (61/88), and 29.6 (26/88) with cooled cells and 66.1 (117/177), 70.1 (82/117), and 13.7 (16/117) with FT cells, respectively. Blastocyst rates of NT embryos derived from FT cells were significantly lower than those from CC or cooled cells (p<0.05). In addition, NT blastocysts produced by using FT cells showed significantly higher apoptosis rates (6.4${\pm}$4.0%) than those produced by CC (2.8${\pm}$1.7%) or cooled (2.3${\pm}$1.3%) cells. However, cooling of donor cells had no significant adverse effect on blastocyst rate as well as apoptosis rate. Therefore, our results suggest that cooled cells may be used as an alternative to freshly cultured confluent culture cells, as donor cells, for the production of Somatic nuclear cloned cattle.

• 한국조경학회지
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• 제30권2호
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• pp.39-49
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• 2002

The purpose of this study is to propose an objective and rational methodology for the selection of proposed sites far the National Trust(NT), which is the new alterative proposal far the conservation of natural environments destroyed by injudicious land development and economic growth. That is to enforce many analysis for the effective estimation of rare ecological and landscape resources and to propose a model based on estimation and united indicators. Using the estimative model, we apply it to the selection of the proposed site in micro scale and simultaneously offer the basic methodology of effective and systematic land conservation in macro scale. The results of this study are as follows: 1) The results of analysis for the reliability of estimative items and indicators, presented no problem in that the coefficient of reliability was over 0.7. 2) The correlation measure of the estimative indicator indicated that 'succession'and 'regenerating restorability' were highly correlative in the item of plants. Another three items showed a tendency to be alike. 3) The results of factor analysis on the characteristics of indicators, classified plants into four categories including a stable factor. The item of animals was classified as a stable and rare factor. The item of landscape was classified as a physical and mental factor and the environment as a pollutional and conditional factor. 4) The model of estimation created through factor analysis was valid for the approval of the regression model because significant probability was 0.00. When we consider the NT proposed site as a complex body that is composed of diverse natural and manmade resources, certainly the synthetic methodology of estimation is needed. If these studies are carried out, NT sites will be selected more rationally and effectively than at present. Consequently, they have the potential to play a core role of natural ecosystem conservation in Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.319-319
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• 2017

Methane is the main greenhouse gas released from rice paddy field. Methane from paddy fields accounts for 11 % of the global total methane emission. The global warming potential (GWP) of methane is 25 times more than that of carbon dioxide on a mass basis. It is well known that most effective practice to mitigate methane in paddy is related to the water management during rice growing season and the use of organic matters. This study was conducted to investigate the effects of tillage and cultivation method on methane emission in paddy. Tillage (tillage and no-tillage) and cultivation methods (transplanting and direct seeding) were combined tillage-transplanting (T-T), tillage-wet hill seeding (T-W), tillage-dry seeding (T-D) and no-till dry seeding (NT-D) to evaluate methane mitigation efficiency. Daily methane emission was decreased on seeding treatments (T-W, T-D, NT-D) than transplanting treatment (T-T). Amount of methane emission during rice growing season is highest in T-T ($411.7CH_4\;kg\;ha^{-1}y^{-1}$) and lowest in NT-D treatment (89.7). In T-W and T-D treatments, methane emissions were significantly decreased by 36 and 51 % respectively compared with T-T. Methane emissions were highly correlated with the dry weight of whole rice plant ($R^2=0.62{\sim}0.93$). T-T treatment showed highest $R^2$ (0.93) among the four treatments. Rice grain yields did not significantly differ with the tillage and cultivation methods used. These results suggest that direct seeding practice in rice production could mitigate the methane emissions without loss in grain yield.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.53-59
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• 2010

A specific deleted version of ARABIDOPSIS RESPONSE REGULATOR1 (ARR1) lacking the signal receiver domain (1.152 amino acids)-coding sequence, referred to as $ARR1{\Delta}DDK$, was amplified using Arabidopsis thaliana cDNA prepared from adult leaves and transferred into the genome of Nicotiana tabacum cv. Samsun under the transcriptional control of a ${\beta}$-estradiol-inducible expression system. The ectopic expression of $ARR1{\Delta}DDK$ affected the morphology of transgenic seedlings and their segments in vitro. In the presence of an inducer, ${\beta}$-estradiol, ectopic expression of $ARR1{\Delta}DDK$ induced only the formation of soft, pseudo-bulbous tissue in the root tip region of intact seedlings, which appeared similar to callus generated on a hypocotyl segment in the presence of 2,4-D and 6-benzyladenine (BA), both at $1\;{\mu}M$. Those callus tissues on the root tip region could not generate shoots unless $1\;{\mu}M$ BA was supplied. In segment culture, ectopic expression of $ARR1{\Delta}DDK$ induced calluslike tissue around the cut-end of cotyledon and hypocotyl segments with occasional shoot formation, suggesting that the expression of $ARR1{\Delta}DDK$ could substitute for the effects of cytokinin on these segments. Additionally, treatment with only ${\beta}$-estradiol induced NtWUS, a WUS ortholog in tobacco, which was detected during the process of callus tissue formation in the root tip region and also in cotyledon or hypocotyl segments. These findings suggest that the NtWUS might be associated in the transdifferentiation process caused by the functional regulation of $ARR1{\Delta}DDK$ in transgenic tobacco seedlings.

• Restorative Dentistry and Endodontics
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• 제29권3호
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• pp.281-287
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• 2004

The purposes of this study were to evaluate the effect of adhesive property on microtensile bond strength and to determine the failure mode. Flat occlusal dentin surfaces were prepared using low-speed diamond saw. The dentin was etched with 37％ phosphoric acid. The following adhesives were applied to the etched dentin to manufacturer's directions： Scotchbond Multi-Purpose in group SM, Prime＆Bond NT in group NT, Scotchbond Multi-Purpose followed by Tetric-flow in group TR. After adhesive application, a cylinder of resin-based composite was built up on the occlusal surface. Each tooth was sectioned vertically to obtain the $1{\;}{\times}{\;}1\textrm{mm}^2$ "sticks". Microtensile bond strength were determined. Each specimen was observed under stereomicroscope and scanning electron microscope (SEM) to examine the failure mode. Data were analyzed using one way ANOVA. The results of this study were as follows：1. The microtensile bond strength value were：group SM ($18.98{\pm}3.01MPa$). group NT ($16.01{\pm}4.82MPa$) and group TR ($17.56{\pm}3.22MPa$). No significant statistical differences were observed among the groups (P>0.05). 2. Most of specimens showed mixed failure. In group TR, there was a higher number of specimens showing areas of cohesive failure in resin.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.215.2-215.2
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• 2015

We fabricated dual active layer (DAL) thin film transistors (TFTs) with indium tin zinc oxide (ITZO) and indium gallium zinc oxide (IGZO) thin film layers using solution process. The ITZO and IGZO layer were used as the front and back channel, respectively. In order to investigate the bias stress stability of ITZO SAL (single active layer) and ITZO/IGZO DAL TFT, a gate bias stress of 10 V was applied for 1500 s under the dark condition. The SAL TFT composed of ITZO layer shows a poor positive bias stability of ${\delta}VTH$ of 13.7 V, whereas ${\delta}VTH$ of ITZO/IGZO DAL TFT was very small as 2.6 V. In order to find out the evidence of improved bias stress stability, we calculated the total trap density NT near the channel/gate insulator interface. The calculated NT of DAL and SAL TFT were $4.59{\times}10^{11}$ and $2.03{\times}10^{11}cm^{-2}$, respectively. The reason for improved bias stress stability is due to the reduction of defect sites such as pin-hole and pores in the active layer.

• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1260-1266
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• 2001

The effect of protein supplementation, $O_2$ concentration and co-culture on the development of embryos produced by nuclear transfer using cultured cumulus cell was investigated. Recipient oocytes and cumulus cells were obtained from the ovaries of the slaughtered Hanwoo cows. Donor cumulus cells were cultured in Dulbecco's modified Eagle medium containing 10% fetal bovine serum at 5% $CO_2$ in air at $38.5^{\circ}C$. The 1 to 6 passages of cumulus cells were isolated and used as donor cells. The in vitro matured oocytes were enucleated and then the isolated donor cells were introduced. One $15{\mu}s$ pulse of 180 volts was applied to induce the fusion between karyoplast and cytoplast. The fused embryos were activated with $10{\mu}M$ calcium ionophore for 5 min and 2 mM 6-dimethylaminopurine for 3 h. To examine the effect of protein supplementation, nuclear transfer (NT) embryos were cultured in one of the following 4 treatments : 1) CR1aa + 3 mg/ml BSA for 7 days ; 2) CR1aa + 10% FBS for 7 days ; 3) CR1aa + 1.5 mg/ml BSA + 5% FBS for 7 days ; and 4) CR1aa + 3 mg/ml BSA for first 3 days and then CR1aa + 1.5 mg/ml BSA + 5% FBS for 4 days. Culture took place at 5% $CO_2$, 5% $O_2$ and 90% $N_2$ at $38.5^{\circ}C$. Although there were no significant differences in cleavage rate among different protein supplements, the rates of blastocyst formation were significantly different. When NT embryos were cultured in the medium supplemented with only BSA, they could develop to only morula not to blastocyst. However, when FBS was supplemented, NT embryos developed to blastocyst stage. In order to investigate the effect of $O_2$ concentration and co-culture, NT embryos were cultured in CR1aa + 1.5 mg/ml BSA + 5% FBS with or without cumulus cell co-culture at an atmosphere of 5% $CO_2$ in air (20% $O_2$) or 5% $CO_2$, 5% $O_2$, 90% $N_2$ (5% $O_2$) at $38.5^{\circ}C$ for 7 days. The percentage of blastocyst development was significantly higher when the NT embryos were cultured at an atmosphere of 5% $O_2$ than that of 20% $O_2$ (p<0.05). However, there was no significant difference between with and without cumulus cell co-culture at an atmosphere of 5% $O_2$ or 20% $O_2$. Fifty embryos were transferred to 25 recipients and 5 recipients were pregnant at 100 days. From 5 pregnant cows, only one cow was delivered of female twin. In conclusion, the embryos reconstructed by enucleation of metaphase II oocytes and introduction of the cycling and quiescent cumulus donor cells in Hanwoo had developmental potential to term after embryo transfer to recipient cows.

• 한국가축번식학회지
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• 제22권4호
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• pp.419-424
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• 1998

토끼 수정란의 전핵에 MT-hGH 유전자를 주입하고 핵이식 기법으로 형질전환 복제수정란의 생산효율과 PCR 검색으로 복제수정란에서 유전자존재 여부를 조사한 바 다음과 같은 결론을 얻었다. 1. MT-hGH 유전자를 주입하여 8- 및 16- 세포기로 자란 수정란을 공핵란으로 사용하여 핵이식을 실시하였던 바, 세포융합률은 각각 60.0%, 62.8% 로 비슷하였으나 정상수정란을 공급핵으로 사용한 80.4% 보다 유의적으로 낮은 융합률을 보였다. 그러나 이들 복제수정란의 체외발달률은 처리군간에 유의적인 차이는 인정되지 않았다. 2. 유전자 주입 후 8- 및 16- 세포기로 자란 수정란의 할구를 이용하여 핵이식으로 복제하고 체외에서 배반포까지 자란 수정란을 PCR -screening으로 유전자를 검출한 결과, 각각 23% 와 33% 의 유전자 양성 수정란을 감별하였다.

• 한국작물학회지
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• 제61권4호
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• pp.251-256
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• 2016

벼 재배시 경운과 재배방법 따른 메탄 발생량 및 토양탄소함량변화를 구명하기위해 경운-이앙, 경운-무논점파, 최소경운-건답직파 및 무경운-건답직파를 비교하는 시험을 수행한 결과 다음과 같다. 1. 메탄 발생량은 경운-이앙 처리구에서 가장 많았으며 경운-무논점파>최소경운-건답직파=무경운 건답직파 순으로 적었다. 2. 메탄의 발생량과 벼 생육과의 관계를 보면 생육초기 작물의 생체량이 많아질수록 메탄 발생량이 증가는 경향을 보였다. 3. 토양 탄소함량은 시험전과 비교하여 경운-이앙 처리구에서 가장 낮은 값을 나타내었고, 최소경운-건답직파와 무경운 처리구에서 높은 값을 나타내었다. 4. 최소경운-건답직파 및 무경운 처리구의 쌀수량이 경운-이앙 처리구에 비해 다소 적었으나 통계적으로 유의한 차이는 없었다.

• 한국작물학회지
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• 제67권1호
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• pp.67-75
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• 2022

Roots play important roles in water and nutrient uptake and in response to various environmental stresses. Investigating diversification of cultivars through root phenotyping is important for crop improvement in adzuki beans. Therefore, we analyzed the morphological and architectural root traits of 22 adzuki bean cultivars using 2-dimensional (2D) root imaging. Plants were grown in plastic tubes [6 cm (diameter) × 40 cm (height)] in a greenhouse from July 25^th to August 28^th. When the plants reached the 2^nd or 3^rd trifoliate leaf stage, the roots were removed and washed with tap water to remove soil particles. Clean root samples were scanned, and the scanned images were analyzed using the WinRHIZO Pro software. The cultivars were analyzed based on six root phenotypes [total root length (TRL), surface area (SA), average diameter (AD), and number of tips (NT) were included as root morphological traits (RMT); and link average length (LAL) and link average diameter (LAD) were included as root architectural traits (RAT)]. According to the analysis of variance (ANOVA), a significant difference was observed between the cultivars for all root morphological traits. Distribution analysis demonstrated that all root traits except LAL followed a normally distributed curve. In the correlation test, the most important morphological trait, TRL, showed a strong positive correlation with SA (r = 0.97^***) and NT (r = 0.94^***). In comparison, between RMT and RAT, TRL showed a significantly negative correlation with LAL (r = -0.50^***); however, TRL did not show a correlation with LAD. Based on RMT and RAT, we identified the cultivars that ranked 5% from the top and bottom. In particular, the cultivar "IT 236657" showed the highest TRL, SA, and NT, while the cultivar "IT 236169" showed the lowest values for TRL, SA, and NT. In addition, the coefficient of variance for the six tested root traits ranged from (14.26-40%) which suggested statistical variability in root phenotypes among the 22 adzuki bean varieties. Thus, this study will help to select target root traits for the adzuki bean breeding program in the future, generating climate-resilient adzuki beans, especially for drought stress, and may be useful for developing biotic and abiotic stress-tolerant cultivars based on better root trait attributes.