• Reproductive and Developmental Biology
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• 제35권3호
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• pp.313-317
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• 2011

This study was conducted to examine the effect of oocyte donor age and micromanipulation medium on the development of mouse cloned embryos receiving cumulus cells. Mouse oocytes were obtained from 6 to 11 week-old mice BDF1 female mice(experiment 1) and cumulus cells were used as donor cells. Micromanipulation procedures for nuclear transfer(NT) were performed in FHM, M2 or Hepes-buffered TCM199(TCM199) medium(experiment 2). After nuclear transfer, the reconstructed oocytes were activated by 10 mM $SrCl_2$ in Ca-free CZB medium in the presence of 5 II ${\mu}$g/ml cytochalasin B for 5 h and cultured in KSOM medium for 4 days. In experiment 1, the survival rate of oocytes after injection of cumulus cells were significantly(p<0.05) lower in oocytes from 6~7 week-old mice(53.3%) than in oocytes from 8~9(80.9%) and 10~11 week-old mice(77.1%). In experiment 2, the survival rate of oocytes after cell injection were significantly(p<0.05) higher in FHM and M2 medium(71.7% and 76.9%) than in TCM199 medium(51.2%). The activation rates of cloned embryos were not different among the micromanipulation media. However, the embryos developed to blastocyst stage were significantly(p<0.05) higher in FHM medium(13.9%) than in M2 and TCM199 medium(0.0% and 0.0%). In conclusion, the present study suggest that oocytes from above 8 week-old mice are superior to oocytes from 6~7 week-old mice as a source of recipient cytoplasm and FHM is superior to M2 and TCM199 as a micromanipulation medium for mouse somatic cell cloning.

• 정보관리학회지
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• 제5권1호
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• pp.53-78
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• 1988

The purpose of this study is to verify the factors determining core journals in a field based upon the results of the citation analysis of the journals in the field of Korean History(KH). In order to verify the determinant factors, dividing the articles of the KH journals into their novelty and author's seniority, the following hypotheses were established. Hypothesis 1: When a KH journal publishes more articles on prehistoric and ancient eras than others, the journal will have higher chance to be included in the core journals. Hypothesis 2: When a KH journal publishes more articles by senior researchers than others, the journal will have higher chance to be included in the core journals. To achieve the purpose, the cited articles found in the two sample journals, namely, Backsan Hakbo and Hanguksa Yongu were analysed during the past 20 years from 1962 to 1981. Applying the Hirst's Discipline Impact Factor method to the the study, Historical Science Impact Factor(HSIF) on the samples itself and the historical era dealt with in the articles and the seniority of the authors during every five-year period. Based upon thr present study to ver~fy the two hypotheses, the following conclusions have been reached. 1) Tht. most influencing sublect mattcr era or1 thix liSIF of the lo~lrual is arranged in prehistoric arid ancient, Koryo, Chosun, recent era, and the others by order of era. 2 ) Clial~g~~lg a fringe or semicore journal u~to a core j ~ u r n a l , very IISIF' 011 it> article e r a 1s grown up arrd prcllistoric a ~ ~ d ancierlt era:. are, s p c c ~ a l l ~ , incr.eased high relatively. Char~ging a core journal int:~ a fr~ngr or semicort. journal, t.very IISIF ti dropped and prel~istc~ric and ancieut taras arc, fallen sharp relatively. 3) The most irlflr~encin~ s~niority 011 the t1SII- of ihc journal I. the senior.., written or1 prehistoric and ancient eras. Above all, the senilir'b HSIF olr prehistoric anti anclent e r a s a r e most ~nfluencing the cliangi~rg of the core journal into the fringe ur bemicore. .i) flypothesis fl is no1 vcrif~ed i r t general. T l ~ e rt, lative connections bet wee^^ the senior's HSIF 011 each era and tht, ~ l ~ a l ~ g i t l g of the tort. ji1ur.tia1 arc \:er~fied in part hut r~ot in the wliole. 5) 'The reason why the 11SIF or1 prehistori~. arid ar~cic~nt e r a s is higl~er than others can be assumed becaltic the new <, xcavations a r r contit~ued actively i l l the prrsr.llt as to the relics arid remains or1 that eras. Accordingly, the HSIF' r i l l thr 11ew 3ubject matter l~ke arcl~acmlogy trends to grow up greatly. Bctween the senior's and juriior's JISIE', junior'.; is higl~er than s., nior's except ancient e r a . That reason can be assumed bccausr Iunlorb t r j to solve the ex~sting problems thr~urglr their ow11 new viewpoints and sights. So, i t should be studied whether the 11SlF on the artic1t.s of the new subject matters and viewpoirrts is influenced to the changing of the core jorlrnal or not.

• Journal of Acupuncture Research
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• 제18권1호
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• pp.170-185
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• 2001

Objective : This experimental study was designed to investigate the effect of Chaenomelis fructus herbal acupuncture on the soleus muscle and tibialis anterior muscle of hindlimb-suspended rats. The measurement have been performed on the composition of type I, type IIa and type IIb muscle fibers in the soleus muscle and tibialis anterior muscie - histochemical study. Materials and Methods : Sprague-Dawley rats weighing about 250g were subjected to hindlimb suspension and divided into six groups; 1. Normal group: normai group 2. Control group: group of hindlimb suspension for 7 days 3. NT group: non-treatment group for 2 or 4 weeks after 7days of hindlimb suspension. 4. EX gtoup: running exercise group for 2 or 4 weeks after 7days of hindlimb suspension. 5. HA group: administration of Chaenomelis fructus herbal acupuncture during 2 or 4 weeks after 7 days of hindlimb suspension. 6. EXHA group: administration of Chaenomelis fructus herbal acupuncture and running exercise during 2 or 4 weeks after 7 days of hindlimb suspension. Results Results : 1. The composition of type I muscle fibers in the soleus muscle significantly increased in 2 or 4 weeks HA and EXHA groups more than control group, and the composition of type IIa muscle fibers in the soleus muscle significantly decreased in 2 or 4 weeks HA and EXHA goups more than control group. 2. The composition of type I muscie fibers in the tibialis anterior muscle significantly increased in 2 or 4 weeks HA and EXHA groups more than control group, and the composition of type IIb muscle fibers in the soleus muscle significantly decreased in 2 or 4 weeks HA and EXHA groups more than control group. 3. The size of type I muscle fibers in the soleus muscle significantly increased in 2 or 4 weeks HA and EXHA groups more than control group, and the size of type IIa muscle fibers in the soleus muscle significantly increased in 2 weeks EXHA group and 4 weeks HA and EXHA groups more than control group. 4. The size of type I, IIa muscle fibers in the tibialis anterior muscle significantly increased in 2 or 4weeks HA, EXHA and EX groups more than control group, and the size of type IIb muscle fibers significantly increased in 2 weeks EXHA group and 4 weeks HA, EXHA and EX groups more than control group. 5. The capiliaries number per fiber of muscle fibers in the soleus and tibialis anterior muscle showed no differences in all experimental groups more than control group. Conclusion : According to the above results, it is shown that Chaenomelis fructus herbal acupuncture could be effective in the treatment of muscle atrophy.

• 대한치과교정학회지
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• 제28권2호
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• pp.189-201
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• 1998

조선대학교 치과대학 학생 중 안모형태와 교합상태가 양호하고, 교정치료의 경험이 없는 정상교합자 74명 (남자 38명, 여자 36명)을 대조군으로 하였고 조선대학교 부속 치과병원 교정과에 내원하여 악교정 수술을 통한 교정치료가 필요하다가 진단된 골격성 III급 부정교합자 성인 45명 (남자 23명, 여자 22명)을 실험군으로 하여 두부규격방사선 사진을 촬영하고 Burstone & Legan방법에 의해 각각의 계측항목별로 남여를 비교 분석하여 악교정 수술이 필요한 골격성 III급 부정교합환자의 악안면 골격 특성에 대해 다음과 같은 결론을 얻었다. 1. 골격성 III급 부정교합 환자는 함몰된 안모를 지니고 있었고 (P<0.001), 여자에서 뚜렷한 상악골 열성장 (P<0.nt)과 하악골 과성장 (P<0.01)을 보인 반면, 남자에서는 통계학적인 유의성이 존재하지 않았고 상,하악골 연조직의 전,후방적인 비교에서는 특징적으로 남자는 하악골 연조직의 과다한 전방돌출 (P<0.001), 여자는 상악골 연조직의 과다한 후퇴 (P<0.001)을 보였는데 이는 골격성 III급 부정교합이 남자는 하악골 과성장, 여자는 상악골 열성장에 의해 주로 기인됨을 알 수 있다. 2. 골격성 III급 부정 교합을 지닌 남여 모두에서 특징적으로 MP-HP 각과 genial angle이 커서 하악골의 전하방 과성장을 보인 반면, ramus height는 남여 모두에서 아무런 영향도 미치지 않았으며, 상악중절치는 순측경사, 하악전치는 설측경사 되어 있었는데 이는 악교정 수술을 위한 술전교정 치료시 이러한 치성보상을 정상으로 회복시켜 각 골격구조내에서 치아들이 적절하게 위치되도록 하는 것이 중요하다고 것을 의미한다. 3. 여자에서 상안면고경에 비해 하안면고경이 특징적으로 짧았고, 남자의 lower facial throat angle이 특징적으로 작았다. 이는 골격성 III급 부정교합이 남자에서는 하악골의 과다한 하방 성장및 긴 목, 여자에서는 하악골의 전방으로의 과다성장및 짧은 목에 기인하는 것으로 보인다.

• The Plant Pathology Journal
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• 제23권3호
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• pp.143-150
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• 2007

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

• Asian-Australasian Journal of Animal Sciences
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• 제26권12호
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• pp.1680-1688
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• 2013

Many different approaches have been developed to improve the efficiency of animal cloning by somatic cell nuclear transfer (SCNT), one of which is to modify histone acetylation levels using histone deacetylase inhibitors (HDACi) such as trichostatin A (TSA). In the present study, we examined the effect of TSA on in vitro development of porcine embryos derived from SCNT. We found that TSA treatment (50 nM) for 24 h following oocyte activation improved blastocyst formation rates (to 22.0%) compared with 8.9% in the non-treatment group and total cell number of the blastocysts for determining embryo quality also increased significantly ($88.9{\rightarrow}114.4$). Changes in histone acetylation levels as a result of TSA treatment were examined using indirect immunofluorescence and confocal microscopy scanning. Results showed that the histone acetylation level in TSA-treated embryos was higher than that in controls at both acetylated histone H3 lysine 9 (AcH3K9) and acetylated histone H4 lysine 12 (AcH4K12). Next, we compared the expression patterns of seven genes (OCT4, ID1; the pluripotent genes, H19, NNAT, PEG1; the imprinting genes, cytokeratin 8 and 18; the trophoblast marker genes). The SCNT blastocysts both with and without TSA treatment showed lower levels of OCT4, ID1, cytokeratin 8 and 18 than those of the in vivo blastocysts. In the case of the imprinting genes H19 and NNAT, except PEG1, the SCNT blastocysts both with and without TSA treatment showed higher levels than those of the in vivo blastocysts. Although the gene expression patterns between cloned blastocysts and their in vivo counterparts were different regardless of TSA treatment, it appears that several genes in NT blastocysts after TSA treatment showed a slight tendency toward expression patterns of in vivo blastocysts. Our results suggest that TSA treatment may improve preimplantation porcine embryo development following SCNT.

• Journal of Ginseng Research
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• 제13권1호
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• pp.42-48
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• 1989

PAH 계 화합물들의 Bay region diol epoxide 들의 target tissue에 대한 결합은 암유발과 관련 되어 있다. 본 논문에서는 ICR mice의 간에서의 BP-DNA-adduct 생성에 미치는 poly acetylene 화합물인 panaxynol 과 panaxydol 의 효과를 조사하였 다. Panaxynol 과 panaxydol 을 전처리한 ICR mice 의 간 마이크로좀을 포함하는 incubation system 은 calf thymus DNA 에 대한 BP binding을 뚜렷이 감소시켰다. [$^3H$]-BP($300\mu$Ci/21nmoles/0.1ml DMSO. i. v. ) 즐 mice 에 주사 후 24시간 후에 간 DNA 에서의 방사능을 측정하였다. HPLC 에 의해 cochromatography 한 두개의 standard marker (acetophenone. bytyrophenone)을 사이에 나타나는 DNA adduct 들을 잠정적으로 확인한 결과 (-) BP-7.8-diol로부터 생성되는 major adduct 인 (+) BP-diol epoxide I: dGuo adduct (peak II)는 대조군보다 약 22% 감소된 반면에 minor adduct 인 (-) BP-diol epoxide I: dGuo adduct (peak III)는 대조군의 69%로 감소되었다. 그리고 (+) BP-7, 8-diol로부 터 생성되는 minor adduct 인 BP-diol epoxide I II : Guo adduct (peak IV)는 대조군의 58%로 감소되었다. 이러한 결과는 panaxydol이 ($\pm$) B BP-7,8-diol로부터 일반적으로 생성되는 adduct들 중 major보다는 minor adduct들의 생성에 더 많이 관석했음을 보여준다.

• 대한토목학회논문집
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• 제31권2D호
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• pp.267-274
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• 2011

한반도에서 기후변화가 가장 현저하게 나타나는 제주지역을 중심으로, 해수면 상승(서귀포 5.6 mm/yr, 제주 5.3 mm/yr)에 따른 해수면 상승고와 대조평균고조시 대형화된 태풍이 내습할 경우에 대한 해수면 상승 시나리오를 작성하고, 수치표고모델 생성 및 GIS공간분석기법을 이용하여, 침수면적, 도로 및 시설물에 대한 영향을 평가하였다. 그 결과, 침수면적은 2040년 2.9 $km^2$, 2070년 4.1 $km^2$, 2100년 최대 5.4 $km^2$으로 나타났다. 도로종류별 침수영향은 군도의 침수가 주를 이루고 있다. 침수시설물은 대부분 단독주택이 차지했으며, 화북동은 단독주택의 침수영향이 높게 나타나 재해에 따른 인명피해가 발생될 가능성이 있다. 또한, 주요시설로서 항만시설의 침수는 물류 및 해운활동에 상당한 영향을 미칠 것으로 사료된다. 본 연구는 해수면 상승에 따른 건설 분야의 영향분석을 통해 정부 산하기관의 전략적인 대응방안 및 정책수립에 활용될 기초자료로 생각된다.

• 대한수의학회지
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• 제36권4호
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• pp.845-858
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• 1996

The purpose of this study was to establish early diagnostic methods for the detection of Aujeszky's disease viral antigens and nucleic acid in nasal cells, and buffy coats from experimentally infected living pigs by a combination of immunocytochemistry, in situ hybridization with digoxigenin(DIG)-labled probe and electron microscopy. Forty days old piglets were inoculated intranasally with $10^{7.0}TCID_{50}$ of Aujeszky's disease virus (ADV, NYJ-1-87 strain). The viral antigens and nucleic acid of ADV were detected in nasal cells, and buffy coat for 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopical method. The results were compared with conventional methods such as a porcine Aujeszky's disease serodiagnostic(PAD) kit, neutralization test(NT) and virus isolation. 1. The viral antigens, nucleic acids and capsids of ADV were detected in nasal cells, buffy coats from 3 days to 20 days after inoculation by immunocytochemistry, in situ hybridization with DIG-labeled probe and electron microscopy, respectively. 2. When viral antigens were detected by the immunocytochemical technique, a diffuse brown deposit was observed in the nucleus and cytoplasm of nasal cells, buffy coats and PK-15 cells under a microscope. 3. DIG-labeled DNA probe was prepared by amplification of conserved sequence of recombinant ADV-gp50 clone with polymerase chain reacction. When ADV-DNA was detected by ISH with DIG-labeled probe, purplish blue pigmentation were observed in the nuclei and cytoplasms of ADV-infected cells under a microscope. Positive signals were observed in nasal cells and in the buffy coat and PK-15 cells at the first day after inoculation. 4. Where ADV-capsids were detected by transmission electron microscopical method, aggregation of capsids was observed in the nuclei and cytoplasms of nasal cells, buffy coats and PK-15 cells. The results suggested that these methods were considered as the highly sensitive and reliable tools for rapid and confirmative diagnosis of Aujeszky's disease in living pigs.

• The Plant Pathology Journal
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• 제35권4호
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• pp.381-388
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• 2019

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.