Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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The Effect of Oocyte Donor Age and Micromainpulation Medium on the Development of Mouse Cloned Embryos

생쥐 복제수정란 발달에 있어서 난자공여 생쥐 연령과 미세조작 배양액의 영향

  • Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Youn-Su (Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo) ;
  • Oh, Keon-Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seong-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 김동훈 (국립축산과학원 동물바이오공학과) ;
  • 이윤수 (동경대학교 의과학연구소) ;
  • 오건봉 (국립축산과학원 동물바이오공학과) ;
  • 황성수 (국립축산과학원 동물바이오공학과) ;
  • 임기순 (국립축산과학원 동물바이오공학과) ;
  • 박진기 (국립축산과학원 동물바이오공학과)
  • Received : 2011.09.02
  • Accepted : 2011.09.09
  • Published : 2011.09.30
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Abstract

This study was conducted to examine the effect of oocyte donor age and micromanipulation medium on the development of mouse cloned embryos receiving cumulus cells. Mouse oocytes were obtained from 6 to 11 week-old mice BDF1 female mice(experiment 1) and cumulus cells were used as donor cells. Micromanipulation procedures for nuclear transfer(NT) were performed in FHM, M2 or Hepes-buffered TCM199(TCM199) medium(experiment 2). After nuclear transfer, the reconstructed oocytes were activated by 10 mM $SrCl_2$ in Ca-free CZB medium in the presence of 5 II ${\mu}$g/ml cytochalasin B for 5 h and cultured in KSOM medium for 4 days. In experiment 1, the survival rate of oocytes after injection of cumulus cells were significantly(p<0.05) lower in oocytes from 6~7 week-old mice(53.3%) than in oocytes from 8~9(80.9%) and 10~11 week-old mice(77.1%). In experiment 2, the survival rate of oocytes after cell injection were significantly(p<0.05) higher in FHM and M2 medium(71.7% and 76.9%) than in TCM199 medium(51.2%). The activation rates of cloned embryos were not different among the micromanipulation media. However, the embryos developed to blastocyst stage were significantly(p<0.05) higher in FHM medium(13.9%) than in M2 and TCM199 medium(0.0% and 0.0%). In conclusion, the present study suggest that oocytes from above 8 week-old mice are superior to oocytes from 6~7 week-old mice as a source of recipient cytoplasm and FHM is superior to M2 and TCM199 as a micromanipulation medium for mouse somatic cell cloning.

Keywords

References

  1. Costa-Borges N, Gonzalez S, Santaló J, Ibáñez E (2011): Effect of the enucleation procedure on the reprogramming potential and developmental capacity of mouse cloned embryos treated with valproic acid. Reproduction 141:789-800. https://doi.org/10.1530/REP-10-0455
  2. Delle Piane L, Lin W, Liu X, Donjacour A, Minasi P, Revelli A, Maltepe E, Rinaudo PF (2010): Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model. Hum Repord 25: 2039-2046. https://doi.org/10.1093/humrep/deq165
  3. Hirabayashi M, Kato M, Takeuchi A, Ishikawa A, Hochi S (2003): Factors affecting premature chromosome condensation of cumulus cell nuclei injected into rat oocytes. J Reprod Dev 49:121-126. https://doi.org/10.1262/jrd.49.121
  4. Inoue K, Ogonuki N, Mochida K, Yamamoto Y, Takano K, Kohda T, Ishino F, Ogura A. Biol Reprod 69:1394-1400.
  5. Kang H, Sung J, Roh S (2011): Development of resversing the usual order of somatic cell nuclear transfer in mice. J Emb Trans 26:85-89.
  6. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y (1998): Eight calves cloned from somatic cells of a single adult. Science 282:2095-2098. https://doi.org/10.1126/science.282.5396.2095
  7. Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Hwang WS, Hossein MS, Kim JJ, Shin NS, Kang SK, Lee BC (2007): Endangered wolves cloned from adult somatic cells. Cloning Stem Cells 9:130-137. https://doi.org/10.1089/clo.2006.0034
  8. Kimura Y, Yanagimachi R (1995): Intracytoplasmic sperm injection in the mouse. Biol Reprod 52:709-720. https://doi.org/10.1095/biolreprod52.4.709
  9. Kishigami S, Mizutani E, Ohta H, Hikichi T, Thuan NV, Wakayama S, Bui T, Wakayama T (2006): Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem Biophys Res Commun 340:183- 189. https://doi.org/10.1016/j.bbrc.2005.11.164
  10. Kishigami S, Wakayama S, Thuan NV, Ohta H, Mizutani E, Hikichi T, Bui H-T, Balbach S, Ogura A, Boiani M, Wakayama T (2006): Production of cloned mice by somatic cell nuclear transfer. Nat Protoc 1:125-138. https://doi.org/10.1038/nprot.2006.21
  11. Liu G, Kato Y, Tsunoda Y (2007): Aging of recipient oocytes reduces the development of cloned embryos receiving cumulus cells. J Reprod Dev 53: 785-790. https://doi.org/10.1262/jrd.18121
  12. Munsie MJ, Michalska AE, O'Brien CM, Trounson AO, Pera MF, Mountford PS (2000): Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei. Curr Biol 10:989- 992. https://doi.org/10.1016/S0960-9822(00)00648-5
  13. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000): Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90. https://doi.org/10.1038/35024082
  14. Rybouchkin A, Heindryckx B, Van der Elst J, Dhont M (2002): Developmental potential of cloned mouse embryos reconstructed by a conventional technique of nuclear injection. Reproduction 124:197-207. https://doi.org/10.1530/rep.0.1240197
  15. Rybouchkin A, Kato Y, Tsunoda Y (2006): Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol Reprod 74: 1083-1089. https://doi.org/10.1095/biolreprod.105.047456
  16. Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L, Westhusin (2002): A cat cloned by nuclear transplantation. Nature 415: 859. https://doi.org/10.1038/nature723
  17. Tsuji Y, Kato Y, Tsunoda Y (2009): The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5- aza-2-deoxycytidine. Zygote 17:109-115. https://doi.org/10.1017/S0967199408005133
  18. Wakayama T, Perry ACF, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. https://doi.org/10.1038/28615
  19. Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P (2001): Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Science 292:740-743. https://doi.org/10.1126/science.1059399
  20. Wakayama T, Yanagimachi R (2001): Mouse cloning with nucleus donor cells of different age and type. Mol Reprod Dev 58:376-383. https://doi.org/10.1002/1098-2795(20010401)58:4<376::AID-MRD4>3.0.CO;2-L
  21. Wells DN, Misica PM, Tervit HR, Vivanco WH (1998):Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby island cattle breed. Reprod Fertil Dev 10:369-378. https://doi.org/10.1071/R98109
  22. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH(1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813. https://doi.org/10.1038/385810a0
  23. Zhou Q, Boulanger L, Renadr JP (2000): A simpliied method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei. Cloning 2:35-44. https://doi.org/10.1089/15204550050145111