• Journal of Ginseng Research
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• v.46 no.6
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• pp.809-818
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• 2022

Background: The non-saponin fraction (NSF) of Korean Red Ginseng is a powder in which saponin is eliminated from red ginseng concentrate by fractionation. In this study, we examined the effect of NSF on age-associated sarcopenia in old mice. Methods: NSF (50 or 200 mg/kg/day) was administered orally daily to young (3-6-month-old) and old (20-24-month-old) C57BL/6 J mice for 6 weeks. Body weight and grip strength were assessed once a week during the oral administration period. The gastrocnemius and quadriceps muscle were excised, and the muscle fiber size was compared through hematoxylin and eosin staining. In addition, the effect of NSF on sarcopenia and inflammation/oxidative stress-related factors in hindlimb muscles was investigated by western blotting. Flow cytometry analysis was conducted to investigate the effect of NSF on immune homeostasis. Blood samples were collected by cardiac puncture, and the serum levels of insulin-like growth factor 1, pro-inflammatory cytokines, and glutathione were evaluated. Results: NSF significantly alleviated muscle strength, mass, and also fiber size in old mice. Age-associated impairment of immune homeostasis was recovered by NSF through retaining CD11b⁺F4/80⁺ macrophages and regulating inflammatory biomarkers. NSF also decreased the age-induced expression of oxidative stress factors. Taken together, NSF showed the effect of improving sarcopenia by inhibiting low-grade chronic inflammatory/oxidative stress factors. Conclusion: NSF exhibited anti-sarcopenia effects by regulating chronic inflammation and oxidative stress in old mice. Thus, we suggest that NSF is a promising restorative agent that can be used to improve sarcopenia in the elderly as well as maintain immune homeostasis.

• Korean Journal of Food Science and Technology
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• v.51 no.1
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• pp.70-80
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• 2019

The aim of this study was to investigate the effects of red ginseng-derived non-saponin fraction (NSF) on inflammatory responses and monocyte-to-macrophage differentiation in RAW264.7 and THP-1. NSF effectively inhibited inflammatory responses by downregulating nitric oxide (NO) production and protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). NSF ($2000{\mu}g/mL$) decreased the levels of NO, iNOS, and COX-2 by 33, 83, and 64%, respectively. NSF inhibited the differentiation of monocyte-to-macrophage by decreasing cell adherence along with downregulation of the cluster of differentiation molecule $11{\beta}$ ($CD11{\beta}$) and CD36. In addition, pro-inflammatory cytokines, such as tumor necrosis factor-alpha, interleukin 6, and monocyte chemoattractant protein 1 (MCP-1), were significantly reduced with NSF treatment. The NSF-mediated inhibition of inflammatory responses was due to the regulation of nuclear factor kappa-light-chain-enhancer of activated B cells ($NF-{\kappa}B$) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2). NSF effectively suppressed the translocation of $NF-{\kappa}B$ into the nucleus, while nuclear Nrf2 and its target protein, heme oxygenase-1, levels were significantly increased.

• Journal of Digital Convergence
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• v.14 no.10
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• pp.473-481
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• 2016

This research aims to examine the relationship between the overseas Koreans' return visit for the participation in the National Sports Festival(NSF) and transnational identity negotiation. The subjects for this study were 378 overseas Koreans who took part in the 96th NSF in 2015. The results are as follows; First, the higher the motivation for socializing with others, maintaining a national identity and self-realization, the more the positive experience in the NSF. However, the higher the motivation for releasing stress and helping business, the more the negative experience they have in the NSF. Second, while the higher the motivation for releasing stress and helping business, the stronger the identity with settlement. However, the higher the motivation for maintaining a national identity, the stronger the identity with origin. Third, while the more the negative experience in the NSF, the stronger the identity with settlement. However, the less the negative experience in the NSF, the stronger the identity with origin.

• Journal of Ginseng Research
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• v.44 no.4
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• pp.655-663
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• 2020

Background: Korean Red Ginseng is known to exhibit immune-enhancing and anti-inflammatory properties. The immune-enhancing effects of the nonsaponin fraction (NSF) of Korean Red Ginseng have been studied in many reports. However, the gastroprotective effect of this fraction is not fully understood. In this study, we demonstrate the activities of NSF for gastrointestinal protection and its related critical factor. Methods: The in vitro and in vivo regulatory functions of NSF on cyclooxygenase-1 (COX-1) messenger RNA and protein levels were examined by reverse transcription polymerase chain reaction and immunoblotting analyses. Gastroprotective effects of NSF were investigated by histological score, gastric juice pH, and myeloperoxidase activity on indomethacin-induced, cold stress-induced, and acetylsalicylic acid-induced gastritis and dextran sulfate sodium-induced colitis in in vivo mouse models. Results: NSF did not show cytotoxicity, and it increased COX-1 messenger RNA expression and protein levels in RAW264.7 cells. This upregulation was also observed in colitis and gastritis in vivo models. In addition, NSF treatment in mice ameliorated the symptoms of gastrointestinal inflammation, including histological score, colon length, gastric juice pH, gastric wall thickness, and myeloperoxidase activity. Conclusion: These results suggest that NSF has gastroprotective effects on gastritis and colitis in in vivo mouse models through COX-1 upregulation.

• Magazine of the Korea Concrete Institute
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• v.7 no.5
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• pp.189-199
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• 1995

Naphthalene sulfonated condensate has been widely used as a superplasticizer for cement and concrete, but the application was limited due to its large slump loss with elapsed time. To complement this demerit of NSF, polycarboxylic acid copolymer from nlaleic anhydride and acryl~c acid(MA) was synthesized to retain the mobility of cement and concrete, and then mixed with NSF. The physical properties, such as fluidity, fluidityretention and rheology, were measured by applying these admixtures to cement paste as a function of elapsed time. And also compressive strength of mortar was measured with curing time. NIv-l and NM-2 containing 10, 20 wt% of MA respectively had a excellent fluidity and a fluidity- retention. In rheological property, the increases of shear stress and viscosity with elapsed time were delayed with the increasing of shear rate in cornparision with NSF only. The marked slump loss of cement paste could be controlled by these admixture. Also the added ainount of admixture and the ratio of water to cement affected these properties.

• Proceedings of the Korea Concrete Institute Conference
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• 1995.04a
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• pp.94-99
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• 1995

Naphthalene sulfonated condensatd(NSF) has been widely using for the superplasticizing of ement and concrete. But NSF has a very large mobility loss with elapsed tiom. To retain mobility of NSF during a certain time, maleic anhydride and acrylic acid copolymer(MA) was polymerized and mixed with NSF in order to perpare admixture holding mobility-retention property of cement. By applying this admixture for ement paste, we examined the fluidity and mobility retention property as a function to elapsed time and measured the compressive strength of mortar with curing time. As a result, NSF containing 20wt% of MA showed very excellent fluidity and mobility-retention property. These properties were affected by the added amount of admixture and the ratio of water to cement.

• Proceedings of the Membrane Society of Korea Conference
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• 2002.11a
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• pp.177-179
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• 2002

• Journal of the Korean Ceramic Society
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• v.37 no.8
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• pp.751-759
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• 2000

Effects of the dosage change, from 0 to 2.0 wt% based on cement weight, of naphthalenic (NSF) and polycarboxylic(NT-2) superplasticizers, on the fluidity of cement paste substituted by 10 wt% II-anhydrite and fly ash respectively as well as II-anhydrite and fly ash itself were investigated. Dispersion properties between particles in suspension were investigated by zeta potential test. Initial fluidity and slump loss in the paste system were observed through mini-slump and apparent viscosity changes with elapsed time. Zeta potential on the particle surface was a tendency to increase according to increasing of NSF dosage. Especially, zeta potential of fly ash has the highest value among all particles equivalent to NSF dosage. In the fluidity of cement paste substituted by inorganic particles, the specimen with substitution of 10 wt% II-anhydrite and fly ash for cement was more effective than cement itself to improve initial fluidity and retain stable fluidity of cement paste. In addition, effect of NT-2 and NSF to improve the fluidity of cement paste, addition of 1.0 wt% NT-2 was more effective than 1.5wt% NSF.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.10c
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• pp.191-196
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• 1998

Under concentrational paste and dilute suspension systems, we have been tested the effects in the dosage of two superplasticizers [naphtalenic(NSF) and polycarboxylic(NT-2)] which has been varied from 0 to 2.0 wt%, in both one case of cement Ⅱ-anhydrite and fly ash itself and the other case that cement has been substituted 10wt% to Ⅱ-anhydrite and fly ash. The result of tested zeta potential tells that zeta potential values increase in addition to increasing of the NSF dosage. And it's effect was high in case of fly ash. We has confirmed the 10wt% substitution of Ⅱ -anhydrite and fly ash would be more effective than the cement itself in initial fluidity and stable fluidity. And in case of NT-2 and NSF, addition of 1.0wt% NT-2 improved the fluidity of paste cement more than that for NSF.

• Applied Chemistry for Engineering
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• v.10 no.4
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• pp.592-598
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• 1999

Exterior finishing materials of artificial stones are manufactured with the mixture of water, cement, stone powder and light-weight aggregate. In this research, we tried to find a way of increasing the physical properties and decreasing the manufacturing cost of artificial stone. So, we used ${\beta}$-NSF base surfactant and vinsol base surfactant to the artificial stone mixture instead of light-weight aggregate. The optimum dosage of the ${\beta}$-NSF and vinsol surfactants for artificial stone are found to be 1.0 wt % of cement, respectively. The physical properties increased ca. 20% and the durability for freezing & thawing of the new artificial stone increased ca. 300%. While the manufacturing cost of the new artificial stone decreased as much as 30%.