• Journal of Korea Water Resources Association
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• v.41 no.4
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• pp.413-422
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• 2008

It is essential to locally adjust the Hargreaves parameter for estimating reference evapotranspiration with short data as a substitute of Penman-Monteith equation. In this study, evaluation of daily-based reference evapotranspiration is computed with Hargreaves equation. in Gyeonggi bay area including Ganghwa, Incheon, Suwon, Seosan, and Cheonan station for the time period of 1997-2004. Hargreaves coefficient is adjusted to give the best fit with Penman-Monteith evapotranspiration, being regarded as a reference. Then, the preferred parameters are validated for the same stations for the time period of 2005-2006. The optimization-based correction in calibration for 1997-2004 shows improved performance of the Hargreaves equation, giving 0.68-0.77 to 0.92-0.98 in Nash-Sutcliffe coefficient of efficiency (NSC) and 14.63-23.30 to 5.23-11.75 in RMSE. The validation for 2005-2006 shows improved performance of the Hargreaves equation, giving 0.43-0.85 to 0.93-0.97 in NSC and 14.43-26.81 to 6.48-9.09 in RMSE.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.20 no.1
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• pp.34-41
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• 2008

Future impact of anthropogenic climate-induced change on ecological regime has been an issue and information on water temperature is required for estimating coastal aquatic environment. One way to induce water temperature is to relate water temperature to air temperature and GCM is able to provide future air temperature data to do this. However, GCM data of low spatial resolution doesn't incorporate local or sitespecific air temperature in need of application, and downscaling processes are essential. In this study, a linear regression is used to relate nationally averaged air temperature to local area for the time period of 2000-2005. The RMSE for calibration (2000-2005) is 1.584, while the RMSE for validation is 1.675 for the year 2006 and 1.448 for the year 2007. The NSC for calibration (2000-2005) is 0.962, while the NSC for validation is 0.955 for the year 2006 and 0.963 for the year 2007. The results show that the linear regression is a good tool to relate local air temperature to nationally averaged air temperature with $1.0{\sim}2.0^{\circ}C$ of RMSE. The study will contribute to estimate future impact of climate-induced change on aquatic environment in Korean coastal zone.

• Journal of Life Science
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• v.28 no.12
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• pp.1397-1405
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• 2018

Mitochondria have multiple functions in cells: providing chemical energy, storing cellular $Ca^{2+}$, generating reactive oxygen species, and regulating apoptosis. Through these functions, mitochondria are also involved in the maintenance, proliferation, and differentiation of stem/progenitor cells. In the brain, the subventricular zone (SVZ) is one of the neurogenic regions that contains neural stem cells (NSCs) throughout a lifetime. However, reports on the role of mitochondria in SVZ NSCs are scarce. Here, we show that rotenone, a complex I inhibitor of mitochondria, inhibits the proliferation and differentiation of SVZ NSCs in different ways. In proliferating NSCs, rotenone decreases mitosis as measured through phosphorylated histone H3 detection; moreover, apoptosis is not induced by rotenone at 50 nM. In differentiating NSCs, rotenone blocks neurogenesis and oligodendrogenesis while glial fibrillary acidic protein-positive astrocytes are not affected. Interestingly, in this study there were more cells in the differentiating NSCs treated with rotenone for 4-6 days than in the vehicle control group which was a different effect from the reduced number of cells in the proliferating NSCs. We examined both apoptosis and mitosis and found that rotenone decreased apoptosis as detected by staining cleaved caspase-3 but did not affect mitosis. Our results suggest that functional mitochondria are necessary in both the proliferation and differentiation of SVZ NSCs. Furthermore, mitochondria might be involved in the mitosis and apoptosis that occur during those processes.

• Journal of Ginseng Research
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• v.45 no.2
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• pp.325-333
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• 2021

Background: Alzheimer's disease (AD) is one of the most prevalent neurodegenerative disorders. Enhancing hippocampal neurogenesis by promoting proliferation and differentiation of neural stem cells (NSCs) is a promising therapeutic strategy for AD. 20(S)-protopanaxadiol (PPD) and oleanolic acid (OA) are small, bioactive compounds found in ginseng that can promote NSC proliferation and neural differentiation in vitro. However, it is currently unknown whether PPD or OA can attenuate cognitive deficits by enhancing hippocampal neurogenesis in vivo in a transgenic APP/PS1 AD mouse model. Here, we administered PPD or OA to APP/PS1 mice and monitored the effects on cognition and hippocampal neurogenesis. Methods: We used the Morris water maze, Y maze, and open field tests to compare the cognitive capacities of treated and untreated APP/PS1 mice. We investigated hippocampal neurogenesis using Nissl staining and BrdU/NeuN double labeling. NSC proliferation was quantified by Sox2 labeling of the hippocampal dentate gyrus. We used western blotting to determine the effects of PPD and OA on Wnt/GSK3β/β-catenin pathway activation in the hippocampus. Results: Both PPD and OA significantly ameliorated the cognitive impairments observed in untreated APP/PS1 mice. Furthermore, PPD and OA significantly promoted hippocampal neurogenesis and NSC proliferation. At the mechanistic level, PPD and OA treatments resulted in Wnt/GSK-3β/β-catenin pathway activation in the hippocampus. Conclusion: PPD and OA ameliorate cognitive deficits in APP/PS1 mice by enhancing hippocampal neurogenesis, achieved by stimulating the Wnt/GSK-3β/β-catenin pathway. As such, PPD and OA are promising novel therapeutic agents for the treatment of AD and other neurodegenerative diseases.

• Biomolecules & Therapeutics
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• v.32 no.1
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• pp.154-161
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• 2024

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a nonessential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the pathogenesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [¹⁴C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1^G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [¹⁴C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [¹⁴C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [¹⁴C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor alpha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection.

• Proceedings of the Korean Institute of Industrial Safety Conference
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• 2000.06a
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• pp.264-269
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• 2000

최근의 농업은 작업의 기계화 등으로 생산성이 높아진 반면, 재해나 건강 문제 등 부작용을 수반하게되었고 그 부작용은 점점 심각한 수준에 이르고있는 반면, 예방측면의 대책은 제조업에 비하여 상대적으로 미미한 수준에 머무르고 있다. 미국의 경우 농업의 재해율(4.3%)은 전 산업평균 재해율의 약1.4배로(NSC,1998) 광업, 건설업 다음의 위험업종이라고 알려져 있고, 일반재해자는 전체의 약 3%임에도 사망자는 전체의 14%나 차지하고 있어서 재해의 강도는 매우 큰 것으로 나타나고 있다. (중략)

• Journal of Korea Foundry Society
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• v.28 no.1
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• pp.41-44
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• 2008

레오다이캐스팅 공정은 용탕상태의 원소재로부터 최종형상에 가장 가까운 정형제품(near-net shape)을 원하는 기계적 성질과 동시에 경제적으로 생산할 수 있는 제조방법으로 개발되어 왔다. 본 논문에서는 전자교반 시스템을 이용하여 결정립이 제어된 반용융 상태의 소재를 수직형 다이캐스팅기를 사용하여 리어파트(rear parts) 자동차 부품을 성형실험 하였다. 성형된 시제품들은 성형조건에 따른 조직 및 인장강도를 관찰하였으며, 시효경화 시간에 따른 T6 최적 열처리 조건을 조사하였다.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.05b
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• pp.357-360
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• 2005

This research investigates the drying shrinkage of non-sintering cement(NSC) matrix added phosphogypsum(PG) and waste lime(WL) to granulated blast furnace slag(GBFS) as sulfate and alkali activators with various curing condition. The experimental results are follow: When the moisture is fully supplied at the early curing age, there is effect which carries out abundant generation of the ettringite which is an expansion nature mineral, and compensates for contraction with a chemical prestress concept.

• Journal of the Korean Professional Engineers Association
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• v.33 no.3
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• pp.59-63
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• 2000

The purpose of this study is to understand the method of scheduling of Petronas twin tower project. Petronas twin tower is the highest building in the world today. Kuala Lumpur City Center Berhad which is owner of Petronas twin tower, managed this construction project by use of useful scheduling techniques, that is, milestone, NSC, NS, PDM, rolling wave, hammock. Now we consider this techniques and try to apply to our construction management.

• Biomolecules & Therapeutics
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• v.27 no.1
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• pp.15-24
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• 2019

Neural stem cells (NSCs) can proliferate and differentiate into multiple cell types that constitute the nervous system. NSCs can be derived from developing fetuses, embryonic stem cells, or induced pluripotent stem cells. NSCs provide a good platform to screen drugs for neurodegenerative diseases and also have potential applications in regenerative medicine. Natural products have long been used as compounds to develop new drugs. In this review, natural products that control NSC fate and induce their differentiation into neurons or glia are discussed. These phytochemicals enable promising advances to be made in the treatment of neurodegenerative diseases.