• 한국패류학회지
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• 제29권3호
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• pp.171-179
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• 2013

동양달팽이의 arginine kinase 유전자는 염기서열 1065개로 이루어져있으며 355개의 아미노산으로 이루어져 있으며, BLAST 결과를 토대로 유사도가 높은 25개의 참고 서열과 동양달팽이의 arginine kinase의 아미노산 서열을 MEGA5 프로그램의 clustalW 모듈을 이용하여 multiple sequence alignment 를 수행한 결과, 연체동물문에 속하는 복족강 (5종), 두족강 (5종), 이매패강 (4종) 등에 속하는 생물들이 같은 군으로 묶였으며, 절지동물문 곤충강 에 속하는 나비목 (2종), 벌목 (1종), 노린재목 (2종) 등에 속하는 생물들이 같은 군으로 묶이고, 갑각강 (5종), 거미강 (1종) 에 속하는 생물들이 묶이는 것을 알 수 있었다. Psipred 소프트웨어를 통해 2D 구조를 비교 분석한 결과도 multiple align 및 phylodendrogram 결과와 밀접한 관계가 있음을 알 수 있었다. 시간에 따른 arginine kinase의 발현양상을 확인한 결과 control에 비하여 6시간에서 약 1.2배 정도 발현이 증가하는 것을 확인할 수 있었으며, 12시간이 지나면 점차 감소하는 것을 확인할 수 있었다. EST를 통해 밝혀진 N. samarangae 의 Ark 서열은 근연종들의 서열과 일치함을 알 수 있었으며, 본 연구 결과를 통해 무척추동물에서의 선천성 면역 관련 유전자 연구에 동양달팽이가 좋은 모델이 될 수 있음을 제시하고 있다.

• Genomics & Informatics
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• 제6권4호
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• pp.173-180
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• 2008

The human genome has evolved as a consequence of evolutionary forces, such as natural selection. In this study, we investigated natural selection on the human genes by comparing the numbers of nonsynonymous (NS) and synonymous (S) mutations in individual genes. We initially collected all coding SNP data of all human genes from the public dbSNP. Among the human genes, we selected 3 different selection groups of genes: positively selected genes (NS/S${\geq}$3), negatively selected genes (NS/S${\leq}$1/3) and neutral selection genes (0.9

• Journal of Acupuncture Research
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• 제21권4호
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• pp.197-206
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• 2004

Objective : This study was designed to find out whether NS(normal saline) is able to be constituted as an appropriate control group for HHT(Hwangryunhaedoktang herbal acupuncture) and HP(Homnis Placenta herbal acupuncture). Methods : NS and two herbal acupuncture were inserted into Quchi(LI 11) of the subjects. After 5 minutes the subjects completed a questionnaire rating the intensity of 21 kinds of acupuncture sensation; hurting, penetrating, sharp, aching, intense, spreading, radiating, tingling, pricking, stinging, pulling, heavy, dull, numb, electric, shocking, hot, burning, cool, pulsing, and throbbing. We compared the subjective acupuncture sensation between the two groups. Results : As for HHT seven items of the acupuncture sensation were significantly different from NS. In case of HP just two items were significantly different from NS. Conclusion : We found that NS may be able to be an placebo herbal acupuncture for HP. Further study is needed for the appropriate placebo herbal acupuncture.

• 전자공학회논문지SC
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• 제38권2호
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• pp.38-49
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• 2001

본 논문은 IC chip내에서 전압을 낮추는 목적으로 사용되는 VDC 회로의 주파수 특성을 향상시키기 위한 새로운 회로를 제안한다. 제안된 회로에는 적응 바이어싱 방법을 통해 저전력소모 및 고속동작을 동시에 만족하는 두 개의 센서와 이 센서로 구동되는 3개의 transistor가 부가적으로 첨가되어 구동 transistor의 gate 충.방진 전류를 보상하여 구동회로의 정상동작을 유지시켜준다. 본 연구에 사용된 회로는 $0.62{\mu}m$ N well CMOS 공정을 사용하였으며, H spice simulation 결과, 내부전압의 변화폭은 부하전류가 0에서 $200m{\Lambda}$까지 5ns동안 증가할 경우 약 1.0V로, $200m{\Lambda}$에서 0으로 감소할 경우 약 0.6V로, 내부전압 회복시간은 증가시 7ns, 감소시 10ns로, 일반적인 구동방식에 비해 성능이 향상되었으며 전체 회로에 소모하는 power는 약 1.2mW로 매우 작았다.

• Natural Product Sciences
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• 제9권1호
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• pp.22-27
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• 2003

This study was designed to investigate the effects of main constituents of Nigella sativa (NS) seed on the survival and CNS responses in experimental animals. The toxicological investigations were conducted for the determination of median lethal doses $(LD_{50})$ of NS seed constituents [i.e. aqueous extract (AE), fixed oil (FO), volatile oil (VO)] and main components of its VO [i.e. thymoquinone (TQ), ${\alpha}-pinene$ (AP) and p-cymene (PC)]. A part of this study includes evaluation NS constituents in the induction of minimal neurological deficit (MND) as a parameter for neurotoxicity using chimney test. In this study, the i.p. $LD_{50}$ values of AE, FO, VO, TQ (suspended In 0.5%CMC), TQ (dissolved in corn oil), AP and PC, were 3020, 3371, 1853, 616.6, 90.3, 1726 and 1523 mg/kg, respectively. All the NS constituents can be considered moderately toxic ($LD_{50}$ ranged from 616.6 to 3371 mg/kg), except the oily solution of TQ, which was very toxic ($LD_{50}$ was 90.3 mg/kg). It appeared that the toxicity of the whole VO is mainly due to its content of TQ and to some extent PC. All the NS constituents induced different degrees of MND at certain dose levels. The median neurotoxic (or sedating) doses $(TD_{50})$ of AE, FO, VO, TQ (suspended in CMC) and AP and PC, were 950, 1403, 306, 88.1, >173 and 368 mg/kg, respectively. TQ was the most potent component in inducing MND, whereas the FO and AE were the least. Neurotoxicity induced by the VO in the chimney test may refer basically to its contents of TQ and to some extent PC and AP.

• 한국식품조리과학회지
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• 제14권4호
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• pp.380-387
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• 1998

The stability of red pepper seed oils during storage at 20${\pm}$3$^{\circ}C$, 40${\pm}$3$^{\circ}C$ and heating at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$ were measured to evaluate red pepper seed oil as a cooking oil. Two species of red pepper seeds (native, improved) were dried by either sunlight or heating to prepare the oil samples of NS (native, sunlight-dried), IS (improved, sunlight-dried), NF (native, heated), and IF (improved, heated). During storage at 20${\pm}$3$^{\circ}C$ or 40${\pm}$ 3$^{\circ}C$, acid values (AV) of all red pepper seed oils were higher than that of soy bean oil (SBO), however, peroxide values (POV) were similar to SBO. Antioxidative stability of NS was better than SBO but IF was not. By the heat treatments at 140${\pm}$3$^{\circ}C$ or 180${\pm}$3$^{\circ}C$, NS was identified to have better antioxidative stability than SBO and IF was the lowest. In sensory evaluation of each deep-fat fried potato-chip at 180${\pm}$5$^{\circ}C$, potato-chips fried in NS were better than that of SBO for color, taste, and flavor. NS-fried potato-chips got the highest score in overall acceptance (p<0.05), however, those of IF showed little acceptance. When blended oils (SBO: NOS, 0, 25, 50, 75%) were used, 50% blended oil was the best for taste, color, flavor, cripness, and total acceptance.

• 대한미생물학회지
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• 제19권1호
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• pp.85-108
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• 1984

In order to study the relationship between resistance of tumor cells to anticancer drugs and immunologic cytotoxicity and their chromosome number, a line of cancer cells (NS-1) was exposed to BCNU and CCNU in vitro. Characteristics of the distribution of chromosome number of the survived cells were then comparatively analyzed. Effect of immune mediated cytotoxicity, i.e. complement and cell-mediated cytotoxicity, on the ploidy characteristics was observed in the same way. NS-1 cells were found to be a population of neoplastic cells of heterogeneity having 5 to 115 chromosomes per cell in metaphase. The majority of the cells were belong to the class of chromosome number 56 to 60 which were considered as the stem cell line. Dramatic changes in the distribution of chromosome number following drug treatment were not observed. However the range of chromosome distribution was slightly changed. Characteristics of chromosomal distribution of drug treated cells were not significantly varied by different doses of drug treated. Changed chromosomal distribution patterns of drug treated cells were reversible, especially the cells having 56 to 60 chromosomes recovered rapidly. Cells having 41-60 and 61-80 chromosomes among cells treated with BCNU and cells with 41-60 chromosomes after CCNU treatment were the major population which regenerated continuously. Following BCNU treatment cells having 61-80 chromosomes were not varied much whereas CCNU treatment affects the population in the same class. Chromosomal aberrations were significantly enhanced by BCNU and CCNU treatment. The frequency of chromosomal aberrations was greater in cells having more than 40 chromosomes compared with that in cells having less than 40 chromosomes. Changes in ploidy characteristics of the cells following complement mediated and cell mediated cytotoxicity were not significant. Therefore it was tentatively concluded that association of numerical distribution pattern of NS-1 cells with the response to the treatment used in this experiment was not recognized.

• Clinical and Experimental Pediatrics
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• 제62권7호
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• pp.274-280
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• 2019

Purpose: To analyze the growth response to growth hormone (GH) therapy in prepubertal patients with Noonan syndrome (NS) harboring different genetic mutations. Methods: Twenty-three patients with prepubertal NS treated at Pusan National University Children's Hospital between March 2009 and July 2017 were enrolled. According to the disease-causing genes identified, the patients with NS were divided into 4 groups. Three groups were positive for mutations of the PTPN11, RAF1, and SOS1 genes. The five genes undetected (FGU) group was negative for PTPN11, RAF1, SOS1, KRAS, and BRAF gene mutations. The influence of genotype was retrospectively analyzed by comparing the growth parameters after GH therapy. Results: The mean chronological age at the start of GH treatment was $5.85{\pm}2.67years$. At the beginning of the GH treatment, the height standard deviation score (SDS), growth velocity (GV), and lower levels of insulin-like growth factor-1 (IGF)-1 levels were not statistically different among the groups. All the 23 NS patients had significantly increased height SDS and serum IGF-1 level during the 3 years of treatment. GV was highest during the first year of treatment. During the 3 years of GH therapy, the PTPN11, RAF1, and SOS1 groups showed less improvement in height SDS, IGF-1 SDS, and GV, and less increase in bone age-to-chronological age ratio than the FGU group. Conclusion: The 3-year GH therapy in the 23 prepubertal patients with NS was effective in improving height SDS, GV, and serum IGF-1 levels. The FGU group showed a better response to recombinant human GH therapy than the PTPN11, RAF1, and SOS1 groups.

• 전력전자학회논문지
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• 제10권6호
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• pp.560-568
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• 2005

제안된 Multi-level PDP sustain Driver는 기존 L. Webber에 의해 제안된 방식에 비해 낮은 전압 rating을 갖는 소자를 사용하며 sustain 전압파형의 rising/falling 구간이 각각 2번의 공진에 의해 이루어진다. 본 논문에서는 rising time을 구성하는 3단계$(T_{r1},\;T_{i1},\;T_{r2})$의 변화에 따라 PDP의 방전 특성에 미치는 영향을 비교하고, 기존 LG전자의 상용화 제품인 42V6와 특성을 비교한다. 실험결과는 3단계의 rising time 중에 $T_{i1}$의 변화에 따른 특성 변화가 가장 크며, $T_{r2}$의 변화에 의한 영향도 있으며, $T_{r1}$의 변화에 따른 특성 변화는 거의 없다. 제안된 PDP driver는 $T_{r1}$이 60ns, $T_{i1}$이 120ns, $T_{r2}$가 350ns 인 경우, Full white display pattern에서 기존제품에 비해 휘도 $14.6\%$증가, 소비전력 $5.9\%$감소, panel 효율 $24.2\%$ 증가, module 효율 $21.2\%$증가 등 특성을 얻을 수 있었다. 실제 PDP module 응용에 적합할 것으로 기대된다.

• 생명과학회지
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• 제34권5호
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• pp.320-332
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• 2024

본 연구는 연소담배와 비교해 서로 다른 가열담배(heated tobacco products, HTPs) 사용 시 혈역학적 지표들의 급성 반응을 비교하고, 급성 HTPs 에어로졸 노출이 심장 자율신경조절(cardiac autonomic regulation, CAR)에 미치는 영향을 탐색하려는 목적으로 수행되었다. 또한, 서로 다른 연초 스틱 간 급성 심혈관 효과의 비교 검증을 목표로 하위연구를 설계했다. 외관상 건강한 남성 흡연자 16명은 개방표지, 무작위 교차시험으로, 최소 48시간 세척기간과 함께 비-흡연(non-smoking, NS), 담배 흡연 및 서로 다른 두 가지 HTPs 사용 세션을 각각 수행했다. 하위연구는 NS와 함께 서로 다른 두 가지 연초 스틱 간 급성 심혈관 효과를 비교하기 위해, 참여자 중 8명을 대상으로 실시되었다. 수축기와 확장기 혈압 및 CAR을 반영하는 심박변이도(heart rate variability, HRV)가 각 세션 시작 전, 제품 사용 중 그리고 사용 후 30분까지 평가되었다. NS 동안 혈역학적 변화는 통계적 차가 없었으나, HTPs 에어로졸에 급성 노출은 담배 연기와 상응하는 수축기와 확장기 혈압의 유의한 증가를 초래했으며, 이들 지표에서 HTPs 기기 간 통계적 차는 없었다. 연소담배와 HTPs 사용 후 통계적으로 유의한 심장 교감신경활동(cardiac sympathetic nerve activity, CSNA)의 급성 증가와 심장 미주신경 긴장도(cardiac vagal tone, CVT)의 급격한 저하가 나타났다. 사용 기기와 독립적으로 HTPs 사용에 따른 CSNA와 CVT 반응은 연소담배와 통계적 차가 없었다. 서로 다른 두 가지 연초 스틱은 NS와 비교해 혈역학적 지표들의 유의한 증가를 유도했으나, 연초 스틱 간 통계적 차는 없었다. CVT는 두 가지 연초 스틱 사용에 따라 유의하게 감소했으며, 연초 스틱의 종류에 따라 CVT 저하에 통계적 차가 있었다. 이상의 결과들은 HTPs 에어로졸 노출이 사용 기기와 독립적으로 연소담배와 동등한 급성 심혈관 효과를 유도할 수 있으며, 연초 스틱의 종류에 따라 급성 CAR 저하가 강화될 수 있음을 시사한다.