• Development and Reproduction
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• v.27 no.3
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• pp.137-147
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• 2023

Pharyngeal pouches are an important epithelial structure controlling facial skeletal development in vertebrates. A series of pouches arise sequentially in the pharyngeal endoderm through collective cell migration followed by rearrangement of pouch-forming cells. While crucial transcription factors and signaling molecules have been identified in pouch formation, a role for Neuropilins (Nrps) in pouch development has not yet been analyzed in any vertebrates. Nrps are cell surface receptors essential for angiogenesis and axon guidance. In all vertebrates, the two Nrp family members, Nrp1 and Nrp2, are conserved in the genome, with two paralogs for Nrp1 (Nrp1a and Nrp1b) and Nrp2 (Nrp2a and Nrp2b) being identified in zebrafish. Here, I report a potential requirement of Nrp signaling in pouch development in zebrafish. nrp1a and nrp2b were expressed in the developing pouches, with sema3d, a ligand for Nrps, being expressed in the pouches. Knocking down Nrps signaling in the pharyngeal endoderm led to severe defects in pouches and facial cartilages. In addition, blocking Mitogen-activated protein kinase (MAPK) activities, a downstream effector of Nrp signaling, in the pharyngeal endoderm caused similar defects in pouches and facial skeleton to those by knocking down Nrps signaling. My results suggest that Nrp signaling acts for pouch formation through MAPK.

• Korea Journal of Hospital Management
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• v.25 no.4
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• pp.60-75
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• 2020

Purpose: This study aims to develop a cost model for NRP (Nursing Residency Program) operation and ultimately provide evidence for financial factors for NRP operation in the future by simulating a cost model. Methodology: This study developed a model for the NRP education cost calculation model based on the review of Hansen's model, which has systematically reported on the development and operation of NRP, and discussions with nursing education experts at a university-affiliated hospital. With the simulation, it was intended to predict nurses' supply and demand in the long term and to calculate changes in long-term education costs. Findings: Firstly, turnover model, term model, cost model necessary for calculating a model for the NRP education cost calculation model was set up. Secondly, the simulation showed the following results; 1) the proportion of newly graduated nurses less than 5 years of working decreases gradually over time, which will make the composition of nurses more balanced. 2) In the first year of the partial introduction of NRP, the cost of training new nurses was about 2.1 times higher than before. After the introduction, the training cost in the 13th year began to be lesser than before the introduction, and in the 25th year, it decreased by 28.1% compared to before the introduction. Practical Implications: Firstly, NRP would be an effective way to solve the higher turnover and frequent departure of new nurses and the imbalance of nurses' composition. Secondly, although the costs of NRP are incurred in the early stages, in the end, NRP training costs are reduced compared to before the introduction of NRP. It is necessary to systematically understand the contribution effect of NRP by analyzing the economic value of NRP considering financial and non-monetary returns in the future and providing a basis for decision-making related to NRP implementation.

• Korean Journal of Applied Biomechanics
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• v.17 no.2
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• pp.23-29
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• 2007

The purpose of this study was to examine if there are kinematic variables differences between national representative players (NRP) and non national representative players (NNRP) during 500 m inline skate starting motion. Four NRP and six NNRP were recruited for the study. Each subject executed starting motion five times on a $2{\times}12m$ start way in a gymnasium. Kinematic variables were analyzed by the three-dimensional motion analysis system (60Hz). It was hypothesized that there are difference in elapsed time and center of mass acceleration in starting phase between groups since starting phase has been considered important in sprinting. The results showed that the NRP had significantly shorter starting phase time than that of NNRP. 1) An elapsed time in phase P1 of NRP was shorter than that of NNRP, and excellent players have early started their first stroke. 2) Both NRP and NNRP have started at the same spot, and displacement of the center of gravity in starting posture of NRP group was at the front compared to NNRP group. 3) Average step lengths of NRP were longer than those of NNRP, and a step change of NRP was stabler compared to that of NNRP. 4) In a speed change of the center of gravity NRP showed comparatively high speed from P1 to P4.

• BMB Reports
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• v.55 no.7
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• pp.348-353
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• 2022

Gastrointestinal cancer is associated with a high mortality rate. Here, we report that the splice variant of NRP/B contributes to tumorigenic activity in highly malignant gastric cancer through dissociation from the tumor repressor, HDAC5. NRP/B mRNA expression is significantly higher in the human gastric cancer tissues than in the normal tissues. Further, high levels of both the NRP/B splice variant and Lgr5, but not the full-length protein, are found in highly tumorigenic gastric tumor cells, but not in non-tumorigenic cells. The loss of NRP/B markedly inhibits cell migration and invasion, which reduces tumor formation in vivo. Importantly, the inhibition of alternative splicing increases the levels of NRP/B-1 mRNA and protein in AGS cells. The ectopic expression of full-length NRP/B exhibits tumor-suppressive activity, whereas NRP/B-2 induces the noninvasive human gastric cancer cells tumorigenesis. The splice variant NRP/B-2 which loses the capacity to interact with tumor repressors promoted oncogenic activity, suggesting that the BTB/POZ domain in the N-terminus has a crucial role in the suppression of gastric cancer. Therefore, the regulation of alternative splicing of the NRP/B gene is a potential novel target for the treatment of gastrointestinal cancer.

• Journal of Korean Society on Water Environment
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• v.23 no.1
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• pp.97-102
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• 2007

The present investigation deals to achieve an accurate determination of the phosphorous present in the wastewater samples using the membrane reactor. The study may enable to quantify the dissolved (DP) and adsorbed phosphorous (AP), also the adsorbed phosphorous categorically identified as inorganic coupled phosphorous (DRP) and organic coupled phosphorous (NRP). Moreover, the study has been conducted separately in anaerobic and aerobic chamber. The results obtained showed that dissolved phosphorous only can occur in anaerobic chamber with ca. 25%. The study conducted for adsorbed phosphorous showed that the DRP has the percent composition in anaerobic and aerobic chamber respectively 33% and 40% i.e., 7% more in aerobic chamber. The similar values obtained for NRP was found to be 42% and 60% i.e., 18% more in aerobic chamber. On the other hand while comparing the results for NRP and DRP, it has to be noted that NRP has 9% and 20% more percent composition than DRP respectively in anaerobic and aerobic chamber. Further, the adsorbed phase showed the species Al-P, Fe-P in the aerobic chamber with the quotient of 7.73 mg/g TS (total solid) whereas in the anaerobic chamber it showed the species Fe-P and $Fe(OH)_3$-P with the 7.16 mg/g TS.

• Proceedings of the IEEK Conference
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• 1998.06a
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• pp.30-33
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• 1998

In this paper, 60 GHz indoor broadband wireless channels are measured with various configurations in a typical coffice environment. Mesurements are taken at nine positions of the room and the base-station antenna is placed either at the center or at an edge of the measurement room, and the remote-station antenna is either sharp beam or broad beam type. The rms delay spread(RDS) and normalized received power (NRP) are estimated from the measurements. Bit error rate simulations are performed using impulse responses for two measurement positions with QPSK/DQPSK OFDM modulation. Using sharp beam antenna results in superior performances than using broad beam antenna in terms of both bit error rates (BER) and NRP penalty. Also, placing the bese-station antenna at the center is superior to placing it at an edge in terms of BER and NRP.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.356-360
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• 2008

As a seedbed for American Public Administration, Traditional Reform Perspective (TRP) produced Wilsonian formulation of administrative science in modern academic setting. From the beginning of the second half of the twentieth century, ideas were transported into Public Administration scholarship. New Reform Perspective (NRP) entails a belief that these reasonings are largely based on assumptions of Economics. Looking into this belief is a necessary condition, if not a sufficient one, for answering the question: Does NRP hold in the study of administration? This initial reading, though somewhat primitive, tries to set up a proposal for identifying a meaningful context for understanding the situation. As a representative version, Wilson's original perspective must be reconsidered in order to articulate the field of study within traditional reform context. According to NRP, new administrative theories are the results of the influence of economic reasoning into the field of Public Administration. Therefore, rereading of Tiebout's 'Pure Theory' and Ostrom, Tiebout and Warrens's 'Theoretical Inquiry' is a prerequisite to satisfy this particular condition.

• Nutrition Research and Practice
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• v.3 no.3
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• pp.253-254
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• 2009

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.4
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• pp.193-198
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• 2003

In the present study, the vestibularly evoked activity of inferior olive (IO) neurons was examined to investigate the vertical vestibular information transmitted through the vestibulo-olivo-cerebellar climbing fiber pathway. The extracellular recording was made in 74 neurons of the IO of cats, while animals were sinusoidally rotated. Most of vestibularly activated IO neurons responded to the vertical rotation (roll) test and were found in or near the ${\beta}$ subnuclei $(IO{\beta})$. The vestibular IO neurons were activated, when the animal was rotated to the side contralateral to the recording site. In contrast to the observation that the gain of responses of yaw sensitive cells (YSC) was not changed by the rotation frequency, that of the roll-sensitive cells (RSC) decreased as the rotation frequency was increased. Regardless of RSC or HSC, IO neurons showed the tendency of phase-lag in their responses. The alternating excitatory and inhibitory phases of responses of RSC were dependent on the direction of head orientation, the characteristics of which are the null response plane (NRP) and the optimal response plane (ORP). The analysis based on the NRP of RSC showed that vestibular inputs from the ipsilateral anterior semicircular canal induced the NRP of the RSC response at about 45 degree counterclockwise to the longitudinal axis of the animal, and that those inputs were distributed to RSC in the rostral part of $IO{\beta}$. On the other hand, those from the posterior semicircular canal were related with the NRP at about 45 degree clockwise and with the caudal part of the $IO{\beta}$. These results suggest that IO neurons receive and encode the vestibular information, the priority of which seems to be the vertical component of the body movement rather than the horizontal ones.

• Journal of Gastric Cancer
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• v.24 no.3
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• pp.300-315
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• 2024

Purpose: Gastric cancer (GC) is among the deadliest malignancies and the third leading cause of cancer-related deaths worldwide. Galectin-1 (Gal-1) is a primary protein secreted by cancer-associated fibroblasts (CAFs); however, its role and mechanisms of action of Gal-1 in GC remain unclear. In this study, we stimulated GC cells with exogenous human recombinant galectin-1 protein (rhGal-1) to investigate its effects on the proliferation, migration, and resistance to cisplatin. Materials and Methods: We used simulated rhGal-1 protein as a paracrine factor produced by CAFs to induce GC cells and investigated its promotional effects and mechanisms in GC progression and cisplatin resistance. Immunohistochemical (IHC) assay confirmed that Gal-1 expression was associated with clinicopathological parameters and correlated with the expression of neuropilin-1 (NRP-1), c-JUN, and Wee1. Results: Our study reveals Gal-1 expression was significantly associated with poor outcomes. Gal-1 boosts the proliferation and metastasis of GC cells by activating the NRP-1/C-JUN/Wee1 pathway. Gal-1 notably increases GC cell resistance to cisplatin The NRP-1 inhibitor, EG00229, effectively counteracts these effects. Conclusions: These findings revealed a potential mechanism by which Gal-1 promotes GC growth and contributes to chemoresistance, offering new therapeutic targets for the treatment of GC.