• Bulletin of the Korean Chemical Society
• /
• 제33권8호
• /
• pp.2609-2612
• /
• 2012

A simple green method was developed for rapid synthesis of silver and gold nanoparticles (AgNPs and AuNPs) has been reported using Lonicera japonica flower extract as a reducing and a capping agent. AgNPs and AuNPs were carried out at $70^{\circ}C$. The successful formation of AgNPs and AuNPs have been confirmed by UV-Vis spectro photometer, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray Analysis (EDAX), scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM). To our knowledge, this is the first report where Lonicera japonica flower was found to be a suitable plant source for the green synthesis of AgNPs and AuNPs.

• 한국언어정보학회:학술대회논문집
• /
• 한국언어정보학회 2007년도 정기학술대회
• /
• pp.57-66
• /
• 2007

Case markers in Korean are omissible in colloquial speech. Previous discourse studies of Caseless bare NPs in Korean show that the information structure of zero Nominative not only differs from that of overt Nominative but it also differs from that of zero Accusative in many respects. This paper aims to provide a basis for these semantic/pragmatic properties of Caseless NPs through the syntactic difference between bare subjects and bare objects: namely, the former are left-dislocated NPs, whereas the latter form complex predicates with the subcategorizing verbs. Our analysis will account for the facts that (i) the distribution of bare subject NPs are more restricted than that of bare object NPs; (ii) bare subject NPs must be specific or topical; (iii) Acc-marked NPs in canonical position tend to be focalized.

• 한국언어정보학회지:언어와정보
• /
• 제11권1호
• /
• pp.53-76
• /
• 2007

Case markers in Korean are omissible in colloquial speech. Previous discourse studies of Caseless bare NPs in Korean show that the information structure of zero Nominative not only differs from that of overt Nominative but it also differs from that of zero Accusative in many respects. This paper aims to provide a basis for these semantic/pragmatic properties of Caseless NPs through the syntactic difference between bare subjects and bare objects: namely, the former are left-dislocated NPs, whereas the latter form complex predicates with the subcategorizing verbs. Our analysis will account for the facts that (i) the distribution of bare subject NPs are more restricted than that of bare object NPs; (ii) bare subject NPs must be specific or topical; (iii) Acc-marked NPs in canonical position tend to be focalized.

• 물과 미래
• /
• 제26권3호
• /
• pp.125-135
• /
• 1993

본 연구의 목적은 도시유역에 적합한 유량 및 수질 모형을 선택하고 그 모형을 임의의 대상유역에 적용하여 그 적합성을 판정하는 데 있다. 본 연구를 위하여 선택된 모형은 EPA의 SWMM 모형으로, 이 모형은 도시유역의 유량 및 수질, 특히 NPS(non-point source) 오염물의 배출의 모의에 적합한 모형이며, 실제 적용에 있어서는 지표면유출을 위해서는 Runoff Block을, 관거 추적을 위해서는 Transport Block을 사용하였다. 본 연구의 적용대상유역은 전형적인 도시유역인 서울시 동대문구 용두유수지 유역이며, 이 유역을 위한 기존의 4개의 연속유량 측정치와 2개의 연속수질 측정치를 가지고 모의를 수행하였다. 유량 및 수질, 특히 NPS 부하량의 검정을 첨두치, 첨두시간, 유출 및 배출 용적과 그들의 상대오차에 대하여 수행하였으며, 그 결과, SWMM 모형은 유출 및 NPS 오염물 배출 모두를 모의하는데 적합한 모형임이 밝혀졌다. 본 연구의 결과는 차후 도시유역의 유출과 NPS 오염물 배출간의 상관관계의 분석 및 그를 통한 유역의 오염물의 년, 월, 부하량의 산정을 통한 물질수지계산 방법의 연구에 바탕이 될 수 있을 것이다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6773-6779
• /
• 2014

Background: Nanotechnology opens new applications in many fields including medicine. Among all metallic nanoparticles, silver nanoparticles (silver NPS) have proved to be the most effective against a large variety of organisms including toxic cyanobacteria. Materials and Methods: Silver NPs were biosynthesized in vivo with different alga species namely, Spirulina piatensis, Chlorella vulgaris and Scenedesmus oh/iquus following two scenarios. First: by suspending a thoroughly washed algae biomass in 1 mM aqueous $AgN0_3$ solution. Second: by culturing them individually in culture media containing the same concentration of $AgN0_3$. Silver NPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FfIR) spectroscopy. The biosynthesized silver NPs were tested for cytotoxic activity against a cancer promoter cyanobacteruim Microcystis aeruginosa, considering effects on cell viability and chlorophyll content. Results: The surface plasmon band indicated the biosynthesis of silver NPs at ~400 nm. Transmission electron microscopy (TEM) revealed that the silver NPs had a mean average size below 100 nm. Energy-dispersive analysis X-ray (EDX) spectra confirmed the presence of silver element. FfIR spectral analyses suggested that proteins and or polysaccharides may be responsible for the biosynthesis of silver NPs and (-COO-) of carboxylate ions is responsible for stabilizing them. The toxic potentialities ofthe biosynthesized silver NPs against the cancer promoter cyanobacterium, Microcystis aeruginosa showed high reduction in viable cells count and the total chlorophyll content. Conclusions: The potential activity of the biosynthesized silver NPs from the studied algae species against Microcystis aernginosa cells is expected to be mainly mediated by the release of silver ions (Ag+) from the particle surface and bioactive compounds as indicated by FfIR analysis.

• 물과 미래
• /
• 제26권4호
• /
• pp.85-95
• /
• 1993

본 연구의 목적은 도시소유역에서의 부족한 유량 및 NPS 오염물 자료의 확충과 이를 통한 유출과 NPS 오염물 배출 간의 상관관계의 분석 및 월, 년 NPS 부하량 산정에 의한 물질수지의 분석에 있다. 본 연구를 위한 자료의 확충을 위해서 미국 EPA의 SWMM 모형을 사용하였으며, 상관관계의 분석 및 물질수지의 분석은 NPS 오염에 가장 영향이 큰 선행괘천기간을 긴 경우(CASE 1.)와 짧은 경우(CASE 2.)로 분리하여 수행하였으며, 연구 결과를 요약하면 다음과 같다. 1) 사상별 시간자료의 분석 : CASE 1.과 CASE 2. 모두, 유량과 농도간의 상관관계 보다는 유량과 부하량간의 상관관계가 좋았으며, 선형 상관관계보다는 비선형 상관관계가 좋았다. 결과적으로 두 CASE 모두 비유량-비부하율 관계는 비선형 산관관계가 가장 좋았다. 2) 사상별 총량자료의 분석 : CASE 1.은 비누적유량-비누적부하량의 선형상관관계가 CASE 2.는 비선형 상관관계가 좋았다. 3) 유역의 NPS 년비부하량 산정 결과, 년평균 단위 강우량당 비부하량은 CASE 1.이 CASE 2.보다 모든 오염물에서 약 2.5배였다. 4) 호우기와 갈수기의 NPS 오염물의 비교 결과, 갈수기의 CASE 1.의 비중이 훨씬 컸다. 5) NPS 년비부하량과 PS 년비부하량을 비교한 결과, NPS 오염물이 유역 및 하천의 오염에 상당히 기여함을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권3호
• /
• pp.1299-1306
• /
• 2014

Background: Nano-biotechnology is recognized as offering revolutionary changes in various fields of medicine. Biologically synthesized silver nanoparticles have a wide range of applications. Materials and Methods: Silver nanoparticles (AgNPs) were biosynthesized with an aqueous extract of Pterocladiella (Pterocladia) capillacea, used as a reducing and stabilizing agent, and characterized using UV-VIS spectroscopy, Fourier Transform Infra red (FT-IR) spectroscopy, transmission electron microscopy (TEM) and energy dispersive analysis (EDX). The biosynthesized AgNPs were tested for cytotoxic activity in a human hepatocellular carcinoma ($HepG_2$) cell line cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, 1% antibiotic and antimycotic solution and 2 mM glutamine. Bacterial susceptibility to AgNPs was assessed with Staphylococcus aureus, Bacillus subtilis [Gram+ve] and Pseudomonas aeruginosa and Escherichia coli [Gram-ve]. The agar well diffusion technique was adopted to evaluate the bactericidal activity of the biosynthesized AgNPs using Ampicillin and Gentamicin as gram+ve and gram-ve antibacterial standard drugs, respectively. Results: The biosynthesized AgNPs were $11.4{\pm}3.52$ nm in diameter. FT-IR analysis showed that carbonyl groups from the amino acid residues and proteins could assist in formation and stabilization of AgNPs. The AgNPs showed potent cytotoxic activity against the human hepatocellular carcinoma ($HepG_2$) cell line at higher concentrations. The results also showed that the biosynthesized AgNPs inhibited the entire panel of tested bacteria with a marked specificity towards Bacillus subtillus. Conclusions: Cytotoxic activity of the biosynthesized AgNPs may be due to the presence of alkaloids present in the algal extract. Our AgNPs appear more bactericidal against gram-positive bacteria (B. subtillus).

• Journal of Microbiology and Biotechnology
• /
• 제31권12호
• /
• pp.1656-1666
• /
• 2021

Dental pathogens lead to chronic diseases like periodontitis, which causes loss of teeth. Here, we examined the plausible antibacterial efficacy of copper nanoparticles (CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. The antimicrobial properties of CME-CuNPs were then assessed against oral microbes (M. luteus. B. subtilis, P. aerioginosa) that cause periodontal disease and were identified using morphological/ biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized, and accordingly, the peak found at 577 nm using UV-Vis spectrometer showed the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents that consequently had a role in Cu reduction and CME-CuNP formation. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates which was superior to the reported values in literature. The antibacterial efficacy of CME-CuNPs on oral bacteria was compared to the synergistic solution of clindamycin with CME-CuNPs. The solution exhibited a superior capacity to prevent bacterial growth. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms were observed between the range of 2.6-3.6 ㎍/ml, 4-5 ㎍/ml and 0.312-0.5, respectively. Finally, the synergistic antimicrobial efficacy exhibited by CME-CuNPs with clindamycin against the tested strains could be useful for the future development of more effective treatments to control dental diseases.

• 한국관개배수논문집
• /
• 제21권1호
• /
• pp.99-108
• /
• 2014

This study investigated the effects of NPS(non point source) pollution reduction of sediment traps through field experiments. Various sizes of 4 sediment traps were applied in a upland field located in Gunwi and assessed the infiltration and storage effects as well as NPS pollution reduction effects of this technique. The characteristics of deposited soil in the sediment traps were also analyzed including distribution of particle size, soil texture, and chemical properties. The results showed that slightly different composition of soil particle size from each sediment trap with high proportion of 0.15mm and 0.25mm ranges of soil particle diameters, while the loamy sand is the main types of deposited soils in the sediment traps. Decreased NPS pollution were observed from the water quality analysis of the samples taken from the sediment traps. Further research need to be proceeded continuously to improve this technique in order to utilize on upland fields for management of agricultural NPS pollutions.

• Advances in nano research
• /
• 제5권3호
• /
• pp.253-260
• /
• 2017

We report a simple eco-friendly process for the synthesis of gold nanoparticles (AuNPs) using aqueous extract from Coffea Arabica fruit. The formation of AuNPs was confirmed using absorption spectroscopy and scanning electron microscopy images. FT-IR analysis demonstrates the major functional groups present in Coffee Arabica fruit extract before and after synthesizing AuNPs. The Face Center Cubic (FCC) polycrystalline nature of these particles was identified by X-Ray diffraction (XRD) analysis. Taking into account the contribution of the biomass surrounding the AuNPs, dynamic light scattering (DLS) results revealed an average particle size of ~59 nm.