• 한국자기공명학회논문지
• /
• 제10권1호
• /
• pp.115-125
• /
• 2006

Hippuryl-L-histidyl-L-leucine(HHL) is widely used as a substrate of angiotensin converting enzyme(ACE) cleaving the neurotransmitter angiotensin(I) to the octapeptide angiotensin(II). The structure of the substrate molecules should provide information regarding the geometric requirements of the ACE active site. For the purpose of determination of in vivo reaction, metallo(Cu, Zn)-HHL complexes were synthesized and the degree of complex formation were identified by MALDITOF, ESI mass spectrometric analysis. Tn addition, the pH-dependent species distribution curves were obtained by potentiometric titration. Nitrogen atoms of imidazole ring and oxygen atom of caboxylate groups in the peptide chain were observed to be participated in the metal complex formation. After purification of complexes further structural characterization were made by utilizing UV-Vis, electrochemical methods and NMR. Complete NMR signal assignments were carried out by using 2D-spectrum techniques COSY, TOCSY, NOESY, HETCOR. A complex that two imidazole and carboxylate groups are asymmetrically participating to coordination mode was predicted to the solution-state structure of $Cu(II)-HHL_2$ based on $^{13}C-NMR$ signal assignment and NOE information.

• 한국자기공명학회논문지
• /
• 제11권1호
• /
• pp.10-23
• /
• 2007

A new disintegrin protein named saxatilin was purified from Korean snake venom (Gloydius saxatilis). Saxatilin is a 73 residue small ploypeptide, which has a primary recognition motif in extracellular matrix, Arg-Gly-Asp (RGD) sequence. Data from inhibition activity assay for the ${\alpha}_v{\beta}_3$ integrin showed that saxatilin showed about 5000-fold higher activity than those of RGD peptides, suggesting that RGD sequence may not be sufficient to induce full cellular function of this site. The solution structures calculated from NMR data were well converged for backbone atoms except RGD loop. The structure revealed that most of tight turns are stabilized by medium range NOE contacts and the RGD motif is located far from the rigid core of the C-terminal domain. The three-dimensional fold and biological function of saxatilin are discussed with those of salmosin, which is a disintegrin protein derived from Agkistrodon halys brevicaudus.

• 한국미생물·생명공학회지
• /
• 제23권6호
• /
• pp.641-646
• /
• 1995

During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR304 which was capable of producing high level of an inhibitor was selected. Based on taxonomic studies, this fungus could be classified as Trichoderma harzianum. The active compound (MR304-1) was purified from culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatographv and HPLC. The inhibitor was identified as 3-(1,5-dihvdroxy-3-isocyanocyclopent-(E)-3-envl)prop-2-enoate by spectroscopic methods of UV, ESIMS, $^{1}$H-NMR, $^{13}$C-NMR, NOE, HMQC and HMBC. MR304-1 showed strong mushroom tyrosinase inhibitory activity with IC$_{50}$ value of 0.25 $\mu $g/ml. It inhibited melanin biosynthesis with 15 mm inhibition zone at 30 $\mu $g/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work. It also inhibited melanin biosynthesis in B16 melanoma cells with a niinimum inhibitory concentration of 0.05 $\mu $g/ml.

• 한국콘텐츠학회논문지
• /
• 제10권10호
• /
• pp.94-101
• /
• 2010

본 논문에서는 SC-FDMA 기술을 적용한 WLED 통신 시스템을 제안하였다. 기존의 WLED 통신 시스템에서는 OFDM 기술을 이용하여 고속의 통신 기술이 구현 가능한 장점이 있지만, 비선형 왜곡에 의한 전송모드에서 PAPR이 발생함에 따라 시스템 성능이 저하되는 단점을 가진다. 더욱이 WLED 장치에서 PAPR에 따른 peak 전력에 의해 정류기가 갑작스럽게 과부하가 발생되면 사람의 시력뿐만 아니라 WLED 시스템에 큰 손상을 미치게 된다. 이를 해결하고자 OFDM에 비해 상대적으로 낮은 PAPR을 갖는 SC-FDMA 기술을 WLED 시스템에 적용하였다. 컴퓨터 시뮬레이션을 통해 본 논문에서 제안하는 WLED 시스템의 PAPR을 측정하고 서로 다른 변조 기법을 적용하였을 경우의 성능 또한 분석한다.

• 한국전자파학회논문지
• /
• 제22권1호
• /
• pp.98-105
• /
• 2011

최대 비 전송 기법에 비해 송신단의 고출력 증폭기에서 발생하는 비선형 왜곡 전달 특성에 강인한 QEGT 코드북 기반의 동 이득 전송 기법에 대해 개선된 인덱스 검색 기법을 제안, 이를 MIMO-OFDM 시스템에 적용한다. 제안하는 검색 기법은 QEGT 코드북을 Q개의 인덱스들로 구성된 M개의 그룹으로 분할한 후, 선택된 최적의 그룹 내 인덱스들 중에서 피드백 인덱스를 결정하는 것으로 기존 검색 기법에 비해 피드백 인덱스 결정을 위한 전체 계산량이 감소하는 장점이 있다. Monte-Carlo 시뮬레이션을 통해 송신 안테나 수가 3~7인 경우, 인덱스 검색 수가 감소하면서도 기존 검색 기법과 거의 동일한 성능을 보임을 확인한다.

• 한국지능시스템학회논문지
• /
• 제13권2호
• /
• pp.222-230
• /
• 2003

의료 시스템이 개발되면 시스템이 사용하는 knowledge source의 신뢰도가 시스템의 수행능력에 큰 영향을 미치게 되므로, knowledge source의 신뢰도를 검증해야한다. 본 논문은 의료 시스템 CLINAID의 knowledge source의 신뢰성 조사에 대한 연구의 방법과 결과를 발표하였다. 그 방법으로는 CLINAID에 사용된 Cardiovascular body system 데이터에 fuzzy relational method를 적용하여 구조적 분석을 통해 만들어진 인공의 syndrome을 knowledge base에 저장되어있는 의료 전문가의 syndrome과 비교하였다. 7 가지 fuzzy implication operator를 사용하여 거의 비슷한 결과들을 산출해 냈으며, 그 결과들이 전문가가 제공한 syndrome과 거의 일치하였다.

• 대한전자공학회논문지TC
• /
• 제48권1호
• /
• pp.62-69
• /
• 2011

LTE 하향링크 시스템에서 새로운 동 이득 전송 코드북 인덱스 탐색기법을 제안한다. 제안하는 탐색기법은 QEGT 코드북을 Q 개의 인덱스들로 구성된 M 개의 그룹으로 분할한 후, 선택된 최적의 그룹 내 인덱스들 중에서 최적의 인덱스를 결정하는 것으로 기존 탐색기법에 비해 인덱스 결정을 위한 전체 계산량이 감소하면서 링크레벨 성능은 유지가 되는 장점이 있다. TI사(社)의 TMS320C6713 DSP보드를 이용한 인덱스 탐색 알고리즘의 구현을 통해 송신 안테나 수가 4인 경우 최적의 피드백 인덱스 결정을 위한 전체 clock-cycle 수가 기존보다 약 25%로 감소됨을 확인한다.

• 대한치과보철학회지
• /
• 제28권2호
• /
• pp.175-182
• /
• 1990

This study was performed to investigate the influence of occlusal change on the masticatory muscle activity. 8 students without any symptom of T.M.J. dysfunction, any history of prosthodontic or orthodontic treatment on dental college of WonKwang Univ. were participated in this study. The activity of masseter and anterior temporal muscles were measured by bioelectric processor(EM2, Myotronics, Inc., U.S.A.) during voluntary maximal clenching on natural teeth, by splint with bilateral posterior surface contact, by splint with unilateral posterior surface contact, and by splint without unilateral posterior teeth contact. The obtained results were as follows ; 1. The loss of posterior contact on noe side resulted in change of the activity of anterior temporal and masseter muscle during clenching on ipsilateral side, but there was no change of muscle activity on contralateral side. 2. The activity of anterior temporal and masseter muscle during clenching were not affected by the pattern of occlusal contact. 3. There were no difference between the activity of anterior temporal and masseter miuscle during clenching by natural teeth and by occlusal splint.

• BMB Reports
• /
• 제42권6호
• /
• pp.387-392
• /
• 2009

Here, we report the first biochemical and structural characterization of the hypothetical protein HP0902 from Helicobacter pylori, in terms of structural genomics. Gel-permeation chromatography and dynamic light scattering indicated that the protein behaves as a dimer in solution. Circular dichroism spectroscopy showed that HP0902 primarily adopts a $\beta$-structure and the protein was highly thermostable with a denaturing temperature higher than $70^{\circ}C$. Finally, the backbone NMR assignments were obtained on the [$^{13}C,^{15}N$]HP0902 and the secondary structure was determined using the chemical shift data. Additionally, the local flexibility was assessed via a heteronuclear $^1H-^{15}N$ steady state NOE experiment. The results revealed that HP0902 would adopt a compactly folded, all-$\beta$ topology with 11 $\beta$-strands. All of the results clearly support the notion that HP0902 belongs to the cupin superfamily of proteins.

• Food Science and Biotechnology
• /
• 제14권2호
• /
• pp.185-189
• /
• 2005

The effect of NaCl solution concentration (from 0 to 3 M) on the extraction of pectinesterase (PE) from jalapeno chili pepper (Capsicum annuum) was studied by determining its solubilization degree from the chili tissue. All concentrations of the salt favored the solubilization of PE in jalapeno chili pepper, compared to that in water. Maximum enzyme activity was obtained with NaCl 2.0 M. The effect of temperature on the PE activity of jalapeno chili pepper in the extracts was also studied. The PE residual activity of jalapeno pepper was 75% after 60 min of incubation at $55^{\circ}C$ and 10% at $75^{\circ}C$. At $85-95^{\circ}C$, PE residual activity was 5% after 5 min of incubation.