Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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MR304-1, A Melanin Synthesis Inhibitor Produced by Trichoderma harzianum

Trichoderma harzianum이 생산하는 melanin 생성 저해물질 MR304-1

  • 이충환 (한국과학기술연구원 생명공학연구소) ;
  • 정명철 (한국과학기술연구원 생명공학연구소) ;
  • 이호재 (한국과학기술연구원 생명공학연구소) ;
  • 이계호 (서울대학교 농업생명과학대학 식품공학과) ;
  • 고영희 (한국과학기술연구원 생명공학연구소)
  • Published : 1995.12.01
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Abstract

During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR304 which was capable of producing high level of an inhibitor was selected. Based on taxonomic studies, this fungus could be classified as Trichoderma harzianum. The active compound (MR304-1) was purified from culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatographv and HPLC. The inhibitor was identified as 3-(1,5-dihvdroxy-3-isocyanocyclopent-(E)-3-envl)prop-2-enoate by spectroscopic methods of UV, ESIMS, $^{1}$H-NMR, $^{13}$C-NMR, NOE, HMQC and HMBC. MR304-1 showed strong mushroom tyrosinase inhibitory activity with IC$_{50}$ value of 0.25 $\mu $g/ml. It inhibited melanin biosynthesis with 15 mm inhibition zone at 30 $\mu $g/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work. It also inhibited melanin biosynthesis in B16 melanoma cells with a niinimum inhibitory concentration of 0.05 $\mu $g/ml.

Keywords

References

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