• 제목/요약/키워드: NO degradation

검색결과 1,431건 처리시간 0.032초

Proteomic Analysis of Diesel Oil Biodegradation by Bacillus sp. with High Phosphorus Removal Capacity Isolated from Industrial Wastewater

  • Hee-Jung Kim;Deok-Won Kim;Jin-Hyeok Moon;Ji-Su Park;Eun-Ji Oh;Jin Yoo;Deok-Hyun Kim;Sun-Hwa Park;Keun-Yook Chung
    • 공업화학
    • /
    • 제34권6호
    • /
    • pp.649-659
    • /
    • 2023
  • This study was initiated to evaluate the phosphorus (P) removal and diesel oil degradation by bacteria isolated from industrial wastewater. The bacteria isolated were identified as Bacillus sp. The P removal efficiencies by Bacillus sp. were 99% at the initial 20 mg/L P concentration. The diesel degradation efficiencies by Bacillus sp. were 86.4% at an initial 1% diesel concentration. Lipophilicity by bacteria was the highest in the log phase, whereas it was the lowest in the death phase. As the diesel was used as a carbon source, P removal efficiencies by Bacillus sp. were 68%. When glucose, acetate, and a mixture of glucose and acetate as second carbon sources were added, the diesel degradation efficiencies were 69.22%, 65.46%, and 51.46%, respectively. The diesel degradation efficiency was higher in the individual additions of glucose or acetate than in the mixture of glucose and acetate. When P concentration increased from 20 mg/L to 30 mg/L, the diesel degradation efficiency was increased by 7% from 65% to 72%, whereas when P concentration was increased from 30 mg/L to 40 mg/L, there was no increase in diesel degradation. One of the five proteins identified by proteome analysis in the 0.5% diesel-treated samples may be involved in alkane degradation and is known as the cytochrome P450 system. Also, two of the sixteen proteins identified in the 1.5% diesel-treated samples may be implicated in the fatty acid transport system and alcohol dehydrogenation.

Evaluation of Mulberry (Morus alba) as Potential Feed Supplement for Ruminants: The Effect of Plant Maturity on In situ Disappearance and In vitro Intestinal Digestibility of Plant Fractions

  • Saddul, D.;Jelan, Z.A.;Liang, J.B.;Halim, R.A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제18권11호
    • /
    • pp.1569-1574
    • /
    • 2005
  • The in situ nylon bag degradation and in vitro intestinal digestibility of dry matter (DM), and crude protein (CP) of mulberry (Morus alba) plant fractions was studied at four harvest stages, 3 (W3), 5 (W5), 7 (W7) and 9 (W9) weeks. Degradability of DM and CP of the whole plant and stem fractions declined significantly (p<0.01) with advancing plant maturity in the order W3>W5 and W7>W9 and W3>W5>W7>W9, respectively. The degradation of DM and CP of the leaf fraction was also influenced by plant maturity but no trend was observed. The degradation of DM and CP of the whole plant and leaves increased rapidly during the first 48 and 24 h of incubation, respectively, when maximum degradation was reached. In vitro intestinal digestibility of CP was more influenced by the residence time in the rumen than by plant maturity. This study showed that mulberry is suitable as a supplement, particularly to low-quality roughages, in providing a source of rapidly available nitrogen to the rumen microbes, hence improving the roughage degradability and intake.

페녹시계 제초제 MCPP [2-(2-Methyl-4-Chlorophenoxy) Propionic Acid]의 미생물학적 분해 (Microbiological Degradation of the Phenoxy Herbicide MCPP [2-(2-Methyl-4-Chlorophenoxy) Propionic Acid])

  • Oh, Kye Heon;Olli H. Tuovinen
    • 미생물학회지
    • /
    • 제30권2호
    • /
    • pp.96-100
    • /
    • 1992
  • 토양세균의 혼합배양에 의한 MCPP [2-(2-Methyl-4-Chlorophenoxy) Propionic Acid]의 미생물학적 분해를 조사하였다. 혼합배양은 Pseudomonas 종, Flavobacterium 종, 그리고 Achromobacter종을 포함하였다. 세균들은 유일한 탄소 및 에너지원으로서 MCPP를 이용하였으나, 부분적인 분해만을 부여주었다. MCPP의 분해는 2-methyl-4-chlorophenol의 형성에 의하여 진행되었으며, 이는 HPLC 에 의하여 발견되었고, GC-MS에 의하여 확인되었다. 이 중간대사물질은 일시적으로 생성되었으며, reverse phase HPLC와 Uv absorbance에 의하여 다른 대사산물의 존재는 관찰되지 않았다.

  • PDF

Effect of Protease Inhibitors on Degradation of Recombinant Human Epidermal Growth Factor in Skin Tissue

  • Ryou, Hae-Won;Lee, Jang-Won;Kyung, Kyung-Ae;Park, Eun-Seok;Chi, Sang-Cheol
    • Archives of Pharmacal Research
    • /
    • 제20권1호
    • /
    • pp.34-38
    • /
    • 1997
  • Recombinant human epidermal growth factor (rhEGF), a polypeptide of 53 amino acid residues, is subject to degradation by numerous enzymes, especially proteases, when it is applied on the skin for the treatment of open wound. Amastatin, aprotinin, bestatin, EDTA, EGTA, gabexate, gentamicin, leupeptin, and TPCK were investigated for the possible protease inhibitors, which may use to protect rhEGF from degradation by the enzymes in the skin. Skin homogenates containing protease inhibitors and rhEGF were incubated at $37^{\circ}C$ for 30 minutes. After the reaction was stopped with trifluoroacetic acid, the amount of rhEGF remaining in the sample was determined with an HPLC method. The percentages of rhEGF degraded, at the skin/PBS ratio of 0.25, in the mouse, rat, and human skin homogenate were 85%, 70%, and 46%, respectively. The degree of degradation of rhEGF in the cytosolic fraction was higher than that in the membrane fraction and these enzyme reactions were completed in 30 minutes. Bestatin, EGTA, and TPCK showed significant inhibitory effects on the degradation of rhEGF in the two fractions (p<0.05), while the other protease inhibitors had no significant inhibitory effects or, even resulted in deleterious effects. Therefore, the formulation containing one or several inhibitors among these effective inhibitors would be a promising topical preparation of rhEGF for the treatment of open wound.

  • PDF

Y$_2$O$_3$ 를 첨가한 정방정 지르코니아에서의 접촉손상 및 강도저하 (Contact Damage and Strength Degradation of Yttria doped Tetragonal Zirconia Polycrystal)

  • 정연길;최성철
    • 한국세라믹학회지
    • /
    • 제35권5호
    • /
    • pp.429-436
    • /
    • 1998
  • The mechanical properties and damage mode of {{{{ {Y}_{2 } {O}_{3} }}-doped tetragonal (Y-TZP) can-didated as biomaterials were performed under indentation stress-strain curve critical load for yield and cracking strength degradation and fatigue behavior with Hertzian indentation tests. This material shows the brittle behavior which is confirmed by indentation stress-strain response. The critical load for cracking(Pc) is much higher than that for yields (Py) indicating crack resistance Strength were strongly dependant on contact area and there were no degradation when the indenter size was ${\gamma}$=3.18 mm suggesting that Y-TZP should be highly damage tolerant to the blunt contacts. Multi-cycle contact were found to be innocuous up to {{{{ {10 }^{6 } }} cycles at 500N and {{{{ {10 }^{5 } }} cycles at 1000N in water. On the other hand contacts at {{{{ {10 }^{6 } }} cycles at 1000 N in water did show some signs of incipient degradation. By contrast contacts with Vickers indenter pro-duced substantial strength losses at much lower loads suggesting that the mechanical integrity of this ma-terial would be compromised by inadvertent sharp contacts.

  • PDF

저전류/저가습 조건에서 고분자전해질 막 열화 (Degradation of Polymer Electrolyte Membrane under Low Current/Low Humidity Conditions)

  • 김태희;이정훈;이호;임태원;박권필
    • 한국수소및신에너지학회논문집
    • /
    • 제18권2호
    • /
    • pp.157-163
    • /
    • 2007
  • During PEMFC operation, low current and low humidity conditions accelerate the degradation of perfluorosulfonic acid membrane. But, there have been no studies that clearly explain why these conditions accelerate the membrane degradation. In this study, the hydrogen permeability through the membrane, I-V polarization of MEA, fluoride emission rate(FER) in effluent water were measured during cell operation under low current densities and low relative humidity(RH). The experimental results were evaluated with oxygen radical mechanism the most commonly known for membrane degradation. It seems that low RH of anode is a good condition for $H{\cdot}$ radical formation on the Pt catalyst and the low current condition accelerates the $H{\cdot}$ to form $HO_2{\cdot}$ radical attacking the polymer membrane.

소규모 농업용 저수지의 노후도 및 안전도 평가 -고삼 저수지에 대한 사례 연구- (Evaluation of Degradation and Safety of Small Agricultural Reservoir)

  • 장병옥;박영곤;우철웅
    • 한국농공학회지
    • /
    • 제40권1호
    • /
    • pp.49-56
    • /
    • 1998
  • Ths study was peformed to evaluate the degree of degradation and safety of a small agricultural reservoir, Kosam Reservoir, in Kyungki Province. Evaluation was done by the program developed by the authors. Results of the study are as follows: 1) Although many burrows were found in downstream side of embankment and cracks were found in wall joining spillway, it appeared that degree of degradation of embankment was in good conditions. 2) Compressive strengths of concrete of crest, side channel, chute floor of spillway were in poor condition. But it appeared that overall degree of degradation of structures was in medium condition based on the criteria of the evaluation system 3) From the analysis of slope stability, safety factor of downstream slope was over 3.3 for the worst condition, such as flood and high water level and that of upstream slope was also over 3.6 for rapid drawdown. In case of earthquake, safety factors were over 2.5 for all conditions. Therefore embankment slopes of Kosam Reservoir were very stable for normal and earthquake condition. 4) As upon assumed failure of embankment of Kosam Reservoir, degree of damage was estimated to be very serious because of many loss of life and properties in the downstream area. 5) Overall grade of safety of Kosam Reservoir was in good condition. Therefore safety was considered to be "No problems" at the present time but further degradation may be proceeded partly and continuously as time goes by.e goes by.

  • PDF

Environmental Quality in Indonesia: Disruption by Economic Agents

  • AZWARDI, Azwardi;SUKANTO, Sukanto;ADNAN, Nazeli;KURNIAWAN, Arika
    • Asian Journal of Business Environment
    • /
    • 제12권1호
    • /
    • pp.17-24
    • /
    • 2022
  • Purpose: This study aims to determine the effect of economic agents, such as the amount of government expenditure on the environment, households, manufacturing industry, and shipping activities; on environmental degradation in Indonesia. Research design, data, and methodology: This study is conducted with 264 observations from panel data of 33 provinces during 2010-2017. Environmental degradation is measured by using the environmental quality index collected from Indonesian Ministry of Forestry and Indonesian Central Bureau of Statistics. Three testing models are used to test the panel data, namely Common Effect Model (CEM), Fixed Effect Model (FEM), and Random Effect Model (REM). Results: The research findings show that the amount of government expenditure on the environment, households, and shipping activities have a negative and significant effects on environmental degradation, while the number of manufacturing industry has positive and significant effect on environmental degradation. Unlike the previous studies, the result also shows that government expenditure on environmental has a positive and significant effect on environmental quality index. Conclusion: It can be concluded that even though Indonesian government spent a low budget on environment, their environmental regulation has succeeded both in reducing environmental degradation and increasing the environmental quality as indicated by Indonesian environmental quality index.