Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Proteomic Analysis of Diesel Oil Biodegradation by Bacillus sp. with High Phosphorus Removal Capacity Isolated from Industrial Wastewater

  • Hee-Jung Kim (Department of Public Administration, Chungbuk National University) ;
  • Deok-Won Kim (Department of Earth Resources and Environmental Engineering, Hanyang University) ;
  • Jin-Hyeok Moon (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Ji-Su Park (EHS Part, Doosan Corporation Electro-Materials) ;
  • Eun-Ji Oh (Water and Land Research Group/Division for Natural Environment, Korea Environment Institute) ;
  • Jin Yoo (Indoor Environment Division, Incheon Research Institute of Public Health and Environment) ;
  • Deok-Hyun Kim (Chemical Accident Investigation Team, National Institute of Chemical Safety) ;
  • Sun-Hwa Park (National Institute of Environmental Research) ;
  • Keun-Yook Chung (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • Received : 2023.08.08
  • Accepted : 2023.11.13
  • Published : 2023.12.10
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Abstract

This study was initiated to evaluate the phosphorus (P) removal and diesel oil degradation by bacteria isolated from industrial wastewater. The bacteria isolated were identified as Bacillus sp. The P removal efficiencies by Bacillus sp. were 99% at the initial 20 mg/L P concentration. The diesel degradation efficiencies by Bacillus sp. were 86.4% at an initial 1% diesel concentration. Lipophilicity by bacteria was the highest in the log phase, whereas it was the lowest in the death phase. As the diesel was used as a carbon source, P removal efficiencies by Bacillus sp. were 68%. When glucose, acetate, and a mixture of glucose and acetate as second carbon sources were added, the diesel degradation efficiencies were 69.22%, 65.46%, and 51.46%, respectively. The diesel degradation efficiency was higher in the individual additions of glucose or acetate than in the mixture of glucose and acetate. When P concentration increased from 20 mg/L to 30 mg/L, the diesel degradation efficiency was increased by 7% from 65% to 72%, whereas when P concentration was increased from 30 mg/L to 40 mg/L, there was no increase in diesel degradation. One of the five proteins identified by proteome analysis in the 0.5% diesel-treated samples may be involved in alkane degradation and is known as the cytochrome P450 system. Also, two of the sixteen proteins identified in the 1.5% diesel-treated samples may be implicated in the fatty acid transport system and alcohol dehydrogenation.

Keywords

Acknowledgement

This research was supported by Chungbuk National University Korea National University Development Project (2022).

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