• Title/Summary/Keyword: NO decay rate

Search Result 103, Processing Time 0.031 seconds

Influence of Arg72 of pharaonis Phoborhodopsin on M-intermediate Decay and Proton Pumping Activity

  • Ikeura, Yukako;Shimono, Kazumi;Iwamoto, Masayuki;Sudo, Yuki;Kamo, Naoki
    • Journal of Photoscience
    • /
    • v.9 no.2
    • /
    • pp.311-313
    • /
    • 2002
  • X-ray structures of pharaonis phoborhodopsin (ppR) show the different direction of the side chain of Arg72 from that of the corresponding residue (Arg82) of bacteriorhodopsin, BR. For BR, this residue is considered to play an important role in the proton pumping. In order to investigate the role of Arg72 in ppR, we constructed Arg72 mutants of R72A, R72K and R72Q, and measured the photocycle and proton pumping activities. The pH-titration curves on the absorption maximum of the mutants were shifted to alkaline in comparison of that of the wild-type. This may imply the increase of pKa of D75, suggesting the presence of the (probably electric) interaction between D75 and Arg72. Rate constants of the M-decay were 3-7 times faster than that of the wild-type, and the time for the completion of the photocycling was also reduced. Using Sn0$_2$ electrode, the rate of transmembrane proton transport was measured upon illumination. The photo-induced proton pumping activities were estimated after the corrections that are the percentages of the associated form of D75 (which has no pumping activity) and the photocycling rates. R72A and R72Q showed the reduced activity while R72K did not reduce the activity.

  • PDF

Storage Quality of Early Harvested Satsuma Mandarin as Influenced by Hot Air Treatment (열풍처리에 따른 조생 온주감글의 저장 중 품질특성 변화)

  • Lee Hyun-Hee;Hong Seok-In;Son Seok-Min;Kim Dongman
    • Food Science and Preservation
    • /
    • v.11 no.3
    • /
    • pp.304-312
    • /
    • 2004
  • Early harvested Satsuma mandarin (Citrus unshiu) was treated with hot air at 45$^{\circ}C$ for 2, 4, and 6 hours and stored at 5$^{\circ}C$ for 3 weeks and additional one week at 18$^{\circ}C$ (simulated shelf-life) in order to examine the effect of mild heat treatment on the storage quality of the citrus fruits. Quality attributes of the sample fruits evaluated during storage included the respiration rate, internal gas composition, pH, titratable acidity, soluble solids content, flesh weight loss, firmness, peel color, decay ratio, and sensory properties. The initial respiration rates, just after hot air treatment, were significantly higher in the heat-treated fruits compared to the untreated. However, during storage at 5$^{\circ}C$, the respiration rates showed a similar level in all treatments. Internal gas composition exhibited no significant difference between the heat-treated and untreated samples. Hot air treatment also exerted no significant effects on the pH, titratable acidity, soluble solid contents, flesh weight loss, firmness, and peel color of the fruits during the whole storage period. The decay ratio was manifestly lower in the heat-treated fruits than the untreated. For sensory attributes including visual and organoleptic quality, no significant difference was observed among sample fruits. Results suggested that mild heat treatment with hot air at 45$^{\circ}C$ for 4-6 hours could be used as an effective preconditioning method to keep the postharvest quality of Satsuma mandarin by reducing the decay incidence remarkably during storage.

Analysis on Ventilation Efficiency of Standard Duck House using Computational Fluid Dynamics (전산유체역학을 이용한 표준 오리사 설계안에 대한 환기효율성 분석)

  • Yeo, Uk-Hyeon;Jo, Ye-Seul;Kwon, Kyeong-Seok;Ha, Tae-Hwan;Park, Se-Jun;Kim, Rack-Woo;Lee, Sang-Yeon;Lee, Seung-No;Lee, In-Bok;Seo, Il-Hwan
    • Journal of The Korean Society of Agricultural Engineers
    • /
    • v.57 no.5
    • /
    • pp.51-60
    • /
    • 2015
  • In Korea, 69.4 % of duck farms had utilized conventional plastic greenhouses. In this facilities, there are difficulties in controlling indoor environments for raising duck. High rearing density in duct farms also made the environmental control difficult resulting in getting more stressed making their immune system weaker. Therefore, a facility is needed to having structurally enough solidity and high efficiency on the environmental control. So, new design plans of duck house have recently been conducted by National Institute of Animal Science in Korea. As a study in advance to establish standard, computational fluid dynamics (CFD) was used to estimate the aerodynamic problems according to the designs by means of overall and regional ventilation efficiencies quantitatively and qualitatively. Tracer gas decay (TGD) method was used to calculate ventilation rate according to the structural characteristics of duck houses including installation of indoor circulation fan. The results showed that natural ventilation rate was averagely 164 % higher than typically designed ventilation rate, 1 AER ($min^{-1}$). Meanwhile, mechanically ventilated duck houses made 81.2 % of summer ventilation rate requirement. Therefore, it is urgent to develop a new duck house considering more structural safety as well as higher efficiency of environmental control.

Mass Loss and Nutrients Dynamics During the Litter Decomposition in Kwangnung Experimental Forest (광릉(光陵) 시험림(試驗林) 내(內)의 임분별(林分別) 낙엽(落葉)의 분해(分解)와 분해과정(分解過程)에 따른 영양염류(營養鹽類)의 변화(變化))

  • You, Young-Han;Namgung, Jeong;Lee, Yun-Young;Kim, Jeong-Hee;Lee, Jong-Young;Mun, Hyeong-Tae
    • Journal of Korean Society of Forest Science
    • /
    • v.89 no.1
    • /
    • pp.41-48
    • /
    • 2000
  • Mass loss and dynamics of mineral nutrient during decomposition of deciduous leaves and 3 species of needles were investigated for 38 months from October in 1992 to November in 1995 in Kwangneung, Korea. After 38 months, the remaining mass of deciduous leaves, Pinus koraiensis, Pinus rigida and Abies holophylla was 16.2%, 29.8%, 33.5% and 53.9%, respectively. The decay rate (k) of deciduous leaves, Pinus koraiensis, Pinus rigida and Abies holophylla was 0.61, 0.40, 0.37, $0.21yr^{-1}$, respectively. The lowest decay rate in fir needle might be, in part, due to low N concentration. N concentration of the decomposing litter increased during the experimental period except for P. rigida. Deciduous leaves showed a short immobilization period during the early stage of decomposition, and big-cone pine and pitch pine had no immobilization period. However, there was no net N mineralization in fir litter. P increased during the experimental period for all litter. Except for deciduous leaves, there was no net mineralization period. In case of deciduous leaves, however, remaining P after 38 months was 53% of the initial P capital. Remaining cations of the decomposing litter after 38 months were lower than those of initial contents.

  • PDF

Wavelet Estimation of Regression Functions with Errors in Variables

  • Kim, Woo-Chul;Koo, Ja-Yong
    • Communications for Statistical Applications and Methods
    • /
    • v.6 no.3
    • /
    • pp.849-860
    • /
    • 1999
  • This paper addresses the issue of estimating regression function with errors in variables using wavelets. We adopt a nonparametric approach in assuming that the regression function has no specific parametric form, To account for errors in covariates deconvolution is involved in the construction of a new class of linear wavelet estimators. using the wavelet characterization of Besov spaces the question of regression estimation with Besov constraint can be reduced to a problem in a space of sequences. Rates of convergence are studied over Besov function classes $B_{spq}$ using $L_2$ error measure. It is shown that the rates of convergence depend on the smoothness s of the regression function and the decay rate of characteristic function of the contaminating error.

  • PDF

Characteristics of Indoor Air Quality of Acidic Air Pollutants in a Private Home During Home During the Summer Season (여름철 가정집에서의 산성오염물질에 대한 실내 공기질 특성)

  • 이학성;강병욱;강충민;여현구
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.17 no.2
    • /
    • pp.193-201
    • /
    • 2001
  • Acidic air pollutants were collected to characterize indoor air quality in a residential area in the summer. All indoor and outdoor samples were measured simultaneously using an annular denuder system(ADS) in Seoul. The data set was collected from July 26 to September 11, 1997. The mean indoor/outdoor ratios measured from this study were : 0.34 for $HNO_3$; 0.91 for $HNO_2$; 0.22 for $SO_2$; 1.34 for $NH_3$; 0.78 for $PM_{2.5}(d_p$ <2.5 $mutextrm{m}$); 0.90 for $SO_{4}^{2-}$; 0.68 for $NO_{3}^{-}$ and 0.79 for $NH_{4}^{+}$. Indoor concentrations of $HNO_3$, $SO_2$ and $PM_{2.5}$ were highly correlated with the outdoor concentrations. The relationship between indoor and outdoor air is dependent, to a large extent, on the rate of air exchange between these two environments. A tracer-gas decay technique with sulfur hexafluoride ($SF_{6}$) as a tracer gas was used to estimate the air exchange of a private home in the summer. The average air exchange rate was estimated to be 23.7 hr(sup)-1. The deposition velocities for $SO_{4}^{2-}$, $NO_{3}^{-}$ and $NH_{4}^{+}$ calculated were 0.17, 0.69 abd 0.39 cm/sec, respectively.

  • PDF

Effect of Anion Generating Air Cleaner on the Components of ETS in a Closed Room (음이온 발생 공기청정기에 의한 밀폐된 실내공간에서의 ETS성분 변화)

  • Hwang, Keon-Joong;Rhee, Moon-Soo;Ra, Do-Young
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.20 no.1
    • /
    • pp.124-130
    • /
    • 1998
  • This study was conducted to evaluate the ability of anion generating air cleaner to remove gases, vapor and particles from closed room contaminated with environmental tobacco smoke (ETS). The measurements covered particle sizes of 13.8-542.5nm, particle concentration, surface area, volumes UVPM, FPM, solanesol, and the following gases and vapor; carbon dioxide, carbon monoxide, nicotine, and 3-ethenylpyridine. Tobacco smoke was generated and mixed in a closed room in which the airflow rates were in the range of 0.00-0.04 m/s. The anion generating air cleaner was startedl and the decay rates for the gases, vapor and particles were measured, When the use of anion generating air cleaner, solid components of ETS, such as respirable suspended particle (RSP), utraviolet particulate matter (UVPM, fluorescent particulate matter (FPM) and solanesol was sharply decreased, and vapor phase components of ETS, such as nicotines 3-ethenylpyidine were modelately decreased by time elapse. Even the use of anion generation air cleaner, the decreasing rate of carbon dioxide concentration was similar with control, and the decreasing rate of carbon monoxide was slower than that of control. Our results indicated that the use of anion generting air cleaner had an effect on reduction of solid and vapor components from ETs, but it had no effect on gaseous components of ETS.

  • PDF

Biomechanical Analysisz of Varying Backpack Loads on the Lower Limb Moving during Downhill Walking (내림 경사로 보행시 배낭 무게에 따른 하지 움직임의 운동역학적 분석)

  • Chae, Woen-Sik;Lee, Haeng-Seob;Jung, Jae-Hu;Kim, Dong-Soo
    • Korean Journal of Applied Biomechanics
    • /
    • v.25 no.2
    • /
    • pp.191-198
    • /
    • 2015
  • Objective : The purpose of this study was to conduct biomechanical analysis of varying backpack loads on the lower limb movements during downhill walking over $-20^{\circ}$ ramp. Method : Thirteen male university students (age: $23.5{\pm}2.1yrs$, height: $175.7{\pm}4.6cm$, weight: $651.9{\pm}55.5N$) who have no musculoskeletal disorder were recruited as the subjects. Each subject walked over $20^{\circ}$ ramp with four different backpack weights (0%, 10%, 20% and 30% of body weight) in random order at a speed of $1.0{\pm}0.1m/s$. Five digital camcorders and two force plates were used to obtain 3-d data and kinetics of the lower extremity. For each trial being analyzed, five critical instants were identified from the video recordings. Ground reaction force, loading rate, decay rate, and resultant joint moment of the ankle and the knee were determined by the inverse dynamics analysis. For each dependent variable, one-way ANOVA with repeated measures was used to determine whether there were significant differences among four different backpack weight conditions (p<.05). When a significant difference was found, post hoc analyses were performed using the contrast procedure. Results : The results of this study showed that the medio-lateral GRFs at RHC in 20% and 30% body weight were significantly greater than the corresponding value in 0% of body weight. A consistent increase in the vertical GRFs as backpack loads increased was observed. The valgus joint movement of the knee at RTO in 30% body weight was significantly greater than the corresponding values in 0% and 10% body weight. The increased valgus moment of 30% body weight observed in this phase was associated with decelerating and stabilizing effects on the knee joint. The results also showed that the extension and valgus joint moments of the knee were systematically affected by the backpack load during downhill walking. Conclusion : Since downhill walking while carrying heavy external loads in a backpack may lead to excessive knee joint moment, damage can occur to the joint structures such as joint capsule and ligaments. Therefore, excessive repetitions of downhill walking should be avoided if the lower extremity is subjected to abnormally high levels of load over an extended period of time.

Quality Analysis of Welsh Onion (Allium fistulosum L.) as Influenced by Storage Temperature and Harvesting Period (대파의 수확기간별 저장온도에 따른 품질 특성)

  • Cha, Hwan-Soo;Youn, Aye-Ree;Kim, Sang-Hee;Jeong, Jin-Woung;Kim, Byeong-Sam
    • Korean Journal of Food Science and Technology
    • /
    • v.40 no.1
    • /
    • pp.1-7
    • /
    • 2008
  • Quality attributes of welsh onion (Allium fistulosum L.) as affected by harvest timing (November, December 2006 and January 2007) and storage temperature (5, 10 and $20^{\circ}C$) were investigated in terms of respiration rate, weight loss, decay rate, color, hardness, sensory quality. A higher respiration rate was found when welsh onions were harvested later and storage temperature was higher. A smaller weight loss was observed in welsh onions that were harvested in November and stored at temperature of $5^{\circ}C$, demonstrating a 9.35% reduction. In other words, there were a minimum difference of 2.15% and a maximum difference of 9.92% between the weight loss in test samples harvested in November and those of test samples harvested in other months. The decay rate was higher in welsh onions harvested in January. The degree of color was more stable in test samples kept at temperature of $5^{\circ}C$ than those kept at higher temperature (10 and $20^{\circ}C$). There were, however, no significant differences in color changes among test samples harvested at different times of the year. Thus color change is closely associated with temperature. Also, welsh onions were harvested in November and stored at temperature of $5^{\circ}C$ showed a good sensory quality.

Analysis of Natural Ventilation Rates of Venlo-type Greenhouse Built on Reclaimed Lands using CFD (전산유체역학을 통한 간척지 내 벤로형 온실의 자연환기량 분석)

  • Lee, Sang-Yeon;Lee, In-Bok;Kwon, Kyeong-Seok;Ha, Tae-Hwan;Yeo, Uk-Hyeon;Park, Se-Jun;Kim, Rack-Woo;Jo, Ye-Seul;Lee, Seung-No
    • Journal of The Korean Society of Agricultural Engineers
    • /
    • v.57 no.6
    • /
    • pp.21-33
    • /
    • 2015
  • Recently, the Korean government announced a new development plan for a large-scale greenhouse complex in reclaimed lands. Wind environments of reclaimed land are entirely different from those of inland. Many standard books for ventilation design didn't include qualitative standard for natural ventilation. In this study, natural ventilation rates were analyzed to suggest standard for ventilation design of venlo type greenhouse built on reclaimed land. CFD (Computational Fluid Dynamics) simulation models were designed according to the number of spans, wind conditions and vent openings. The wind profile at a reclaimed land was designed using ESDU (Engineering Sciences Data Unit) code. Using the designed CFD simulation model, ventilation rates were computed using mass flow rate and tracer gas decay method. Additionally computed natural ventilation rates were evaluated by comparing with ventilation requirements. As a result of this study, ventilation rates were decreased with increasing of the number of spans. Ventilation rates were linearly increased with increasing of wind speed. When the wind speed was $1.0\;m{\cdot}s^{-1}$, only side vent was open and wind direction was $45^{\circ}$, homogeneity of ventilation rate at 0~1 m height is the worst. Finally, chart for computing natural ventilation rate was suggested. The chart was expected to be used for establishing standard of ventilation design.