• 대한바이러스학회지
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• 제26권2호
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• pp.191-204
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• 1996

The characterization of the 5 Korean isolates (K96P10, K94P05, K91P55, K87P39, and K82P01) of Japanese encephalitis virus (JEV) was compared with JE virus prototype Nakayama-NIH (NKY-NIH) using prM/M and envelope gene sequences of the JEV genome and phylogenetic analysis. The antigenic analysis of these viruses were done by the cross-hamagglutination inhibition (HI) test using polyclonal antibodies against Korean isolates and NKY-NIH. The sequence homology of the Korean isolates and NKY-NIH ranged between 87.4 % - 95.6 % at the nucleotide level and between 98.2 % - 97.2 % at the amino acid level over the E nucleotides compared. Alignment of E protein amino acid sequences revealed that residue positions E89, E129, E221, E244, E327, E366, E459, and E477 characterized the Korean strains. According to phylogenetic analysis bases on the E nucleotide, there are at least 2 genetic types of JEV existing in Korea and Korean strains were distinct from NKY-NIH. However, the cross HI test results of all the Korean isolates were serologically no different from NKY-NIH strain.

• Toxicological Research
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• 제16권2호
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• pp.173-178
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• 2000

This study was carried out to investigate cytotoxicity of several herbicides (Bentazone, Butachlor. Paraquat and Ethalfluralin) in cultured mouse NIH 3T3 fibroblasts. Tetrazolium (MTT), neutral red (NR) and sulforhodamine protein B (SRB) of the colorimetric assays were performed to evaluate the cytotoxicity on cell organelles. 2 x 10$^4$cell/$m\ell$ of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution (1, 25, 50 or 100 $\mu$M) of each herbicide. After the NIH 3T3 fibroblasts of all groups were cultured in the same condition for 48 hours, MTT, NR and SRB assays were performed to evaluate the cytotoxicity. The light microscopic study was carried out to examine morphological changes of cultured NIH 3T3 fibroblasts. The MTT$_{50}$ of Bentazone, Butachlor, Paraquat and Ethalfluralin were 1560.97 $\mu$M, 56.15 $\mu$M, 3138.81 $\mu$M and 1301.82 $\mu$M, respectively. The NR$_{50}$ of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1763.93 $\mu$M, 45.98 $\mu$M, 1030.85 $\mu$M and 1808.29 $\mu$M, respectively. The SRB$_{50}$ of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1913.38 $\mu$M, 65.30 $\mu$M, 1860.73 $\mu$M and 1086.93 $\mu$M, respectively. The morphological changes of NIH 3T3 fibroblasts showed severe degeneration in Butachlor 50 $\mu$M and 100 $\mu$M concentrations. These results indicate that Butachlor has high cytotoxicity, Bentazone, Paraquat and Ethalfluralin very weak cytotoxicity against NIH 3T3 fibroblasts.lasts.

• 대한한방내과학회지
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• 제36권3호
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• pp.391-399
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• 2015

목적 본 증례 보고에서는 보중익기탕 가감방인 배농익기산의 만성 전립선염/만성 골반 동통 증후군의 치료효과를 보고한다.방법 본 원(청춘 한의원)에 내원한 만성 전립선염/만성 골반 동통 증후군 11명 환자에게 배농익기산을 처방한 효과적인 증례를 보고한다. 증상의 변화는 미국 국립보건원 만성전립선염 증상 점수표(NIH-CPSI)로 측정하였다.결과 배농익기산 처방 후 NIH-CPSI에서 통계학적으로 유의미한 감소가 나타났다. NIH-CPSI 총점의 평균은 치료 전 37.6±5.8점에서 치료 후 7.5±3.9 점으로 감소하였다(p<0.001). NIH-CPSI 총점은 평균 30.2±7.3 점 감소하였다. 배농익기산 처방 후 NIH-CPSI의 총점, 통증 점수, 배뇨증상 점수, 삶의 질 점수에서 모두 유의미한 감소가 나타났다.결론 배농익기산은 만성 전립선염/만성 골반 동통 증후군을 치료하는 매우 효과적인 한약이다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2002년도 추계학술대회 및 총회
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• pp.144-144
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• 2002

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.329.2-329
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• 1998

No Abstract, See Full Text

• 암석학회지
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• 제11권3_4호
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• pp.250-258
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• 2002

석영의 파동소광은 암석의 변형도를 나타낼 수 있는 좋은 지표로서 사용할 수 있다 암석의 변형도를 결정하기 위해 석영의 파동소광 강도(IWE)를 편광현미경, 디지털 카메라, NIH Image로 측정하였다. 본 연구 에서는 이러한 방법을 이용하여 복잡한 변형을 받은 청산지역일대의 청산반상화강암, 청산복운모화강암, 백록화강암의 변형도를 구하여 청산일대의 변형사를 밝혔다. NIH Image로 측정한 결과, 청산화강암과 백록화강암 사이의 주향이동단층 부근과 청산화강암과 영동층군 사이의 부정합면 부근에서 높은 변형도가 나타났다. 따라서 이 지역의 최근의 주된 변형 요인은 영동분지를 형성한 단층과 청산화강암과 백록화강암 사이에 있는 주향이동단층이다.

• 대한미생물학회지
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• 제22권1호
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• pp.71-78
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• 1987

In order to determine the virulence properties of eleven strains of Sh. flexneri isolated from diarrheal patients the congo red test, the $Ser{\acute{e}}ny$ test, the HeLa cell invasion test and electrophoresis of plasmids were carried out. The results were summarized as follows. 1. Virulent strains were not determined by the result of Congo red absorption test. 2. Virulent strains showed positive reaction by the Sereny test and the HeLa cells invasion, but avirulent strains revealed negative reaction at those tests. 3. The temperature condition of bacterial growth was a factor of virulent expression. 4. Virulent strains were mostly possessed of a 130.3 Mdal plasmid, but avirulent strains were not.

• 대한의생명과학회지
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• 제14권3호
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• pp.187-192
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• 2008

To clerify the protective effect of omega-3 of polyunsaturated fatty acid docosahexaenoic acid (DHA) on the cytotoxicity induced by organic mercury in cultured NIH3T3 fibroblasts. The measurement of cell viability on ogranic mercury wad done by XTT assay after NIH3T3 fibroblasts were cultured with various concentrations of methyl mercuric chloride (MMC). And also, the effect of DHA on the MMC-mediated cytotoxicity was examined by cell viability, and antioxidant effect of DHA was also assessed by superoxide dismutase (SOD)-like activity and the lipid peroxidation activity in cultured NIH3T3 fibroblasts. In this study, MMC decreased cell viability and $XTT_{50}$ value was determined at $50{\mu}M$ of MMC in these culture. In the effect of DHA against the cytotoxicity induced by MMC, DHA significantly increased the cell viability damaged by MMC in cultured NIH3T3 fibroblasts. And also, DHA showed the antioxidant effect by showing the increase of SOD-like activity and the decrease of lipid peroxidation activity. From these results, it is suggested that organic mercury such as MMC has highly toxic effect on cultured NIH3T3 fibroblasts, and also, omega-3 of polyunsaturated fatty acid, DHA showed the protection on MMC-induced cytotoxicity and antioxidant effect.

• 생명과학회지
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• 제12권2호
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• pp.182-187
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• 2002

본 연구에서는 알킬화제인 methylmethane sulfonate (MMS)를 선처리한 NIH3T3세포에서 소목추출물의 효과를 분석하였다. MTT 분석결과 MMS에 의해서 유도된 세포생존률이 소목추출물에 의해서 감소되었다. 세포형태분석, acridine orange 염색법, 그리고 DNA fragmentation 분석에서 MMS에 의해서 유도된 세포고사의 특징인 핵 응축 및 DNA laddering이 소목추출물에 의해서 증가됨이 관찰되었다. 이러한 결과들로 소목추출물은 NIB73T3 세포에서 MMS에 의해서 유도된 세포고사를 촉진시킴을 보여준다.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.169-176
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• 2007

Objectives : The purpose of this study was to investigate inhibitory effect of Gagamtongsung-San(GTS) on the cancer. Methods : This study estimated the cytotoxicity of GTS about L1210 and NIH3T3. We used GTS extract distilled with water, n-Hexane, Ethyl acetate and Butanol. The cytotoxicitys of GTS about cancer cells and normal cells were tested using a colorimetric tetrazoliun assay(MTT assay). Results : The results of this study were obtained as follow ; l. Cytotoxicity of water extract of GTS in L1210 cell lines was significantly increased, compared with NIH3T3. 2. n-Hexane fraction of GTS had similar cytotoxicity between L1210 and NIH3T3, and that have similar $IC_{50}$ of water extract of GTS at 276 ${\mu}g/ml$ 3. Ethyl acetate fraction of GTS had low degree cytotoxicity both L1210 and NIH3T3 cell lines. 4. Butanol fraction of GTS had cytotoxicity between L1210 and NIH3T3. Significantly, Cytotoxicity of GTS in L1210 cell lines was significant increased. 5. $H_2O$ fraction of GTS had no cytotoxicity both L1210 and NIH3T3.