Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Extracts of Caesalpina sappan L. Potentiate the Apoptosis of NIH3T3 Cells Exposed to Methymethane Sulfonate

알킬화제인 MMS를 선처리한 NIH3T3 세포에서 소목 추출물 의한 세포고사의 촉진

  • 박종군 (원광대학교 자연대 생명과학부) ;
  • 황성진 (원광대학교 자연대 생명과학부) ;
  • 이정섭 (조선대학교 자연대 생물과학부) ;
  • 전병훈 (원광대학교 한의과대학) ;
  • 김원신 (원광대학교 자연대 생명과학부)
  • Published : 2002.04.01
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Abstract

In this study we have investigated the effect of Caesalpina sappann L. extracts on the apoptosis in NIH3T3 cells exposed to methylmethan sulfonate (MMS), an alkylating agent. MTT assay study showed that Caesalpina sappan L. extracts potentiate the MMS-induced viability. Cell morphology studies, acridine orange (AO) staining, and DNA fragmentation analysis indicated that the postincubation of Caesalpina sappan L. extracts increase the nuclear condensation of MMS-induced apoptotosis. These results suggest that Caesalpina sampan L. extracts contain components potentiating MMS-induced apoptosis of NIH3T3 cells.

본 연구에서는 알킬화제인 methylmethane sulfonate (MMS)를 선처리한 NIH3T3세포에서 소목추출물의 효과를 분석하였다. MTT 분석결과 MMS에 의해서 유도된 세포생존률이 소목추출물에 의해서 감소되었다. 세포형태분석, acridine orange 염색법, 그리고 DNA fragmentation 분석에서 MMS에 의해서 유도된 세포고사의 특징인 핵 응축 및 DNA laddering이 소목추출물에 의해서 증가됨이 관찰되었다. 이러한 결과들로 소목추출물은 NIB73T3 세포에서 MMS에 의해서 유도된 세포고사를 촉진시킴을 보여준다.

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