• Korean Journal of Environmental Biology
• /
• v.39 no.4
• /
• pp.415-422
• /
• 2021

Cotton is an important fiber crop, and its seeds are used as feed for dairy cattle. Crop biotechnology has been used to improve agronomic traits and quality in the agricultural industry. The frequent unintentional release of LM cotton into the environment in South Korea is attributed to the increased application of living modified (LM) cotton in food, feed, and processing industries. To identify and monitor the LM cotton, a method for detecting the approved LM cotton in South Korea is required. In this study, we developed a method for the simultaneous detection of four LM cotton varieties, MON757, MON88702, COT67B, and GHB811. The genetic information of each LM event was obtained from the European Commission-Joint Research Centre and Animal and Plant Quarantine Agency. We designed event-specific primers to develop a multiplex PCR method for LM cotton and confirmed the specific amplification. Using specificity assay, random reference material(RM) mixture analysis and limit of detection(LOD), we verified the accuracy and specificity of the multiplex PCR method. Our results demonstrate that the method enabled the detection of each event and validation of the specificity using other LM RMs. The efficiency of multiplex PCR was further verified using a random RM mixture. Based on the LOD, the method identified 25 ng of template DNA in a single reaction. In summary, we developed a multiplex PCR method for simultaneous detection of four LM cotton varieties, for possible application in LM volunteer analysis.

• Proceedings of the Zoological Society Korea Conference
• /
• 2001.10a
• /
• pp.273.1-273
• /
• 2001

• Proceedings of the Zoological Society Korea Conference
• /
• 2001.10a
• /
• pp.272.3-273
• /
• 2001

• Proceedings of the KHMA Conference
• /
• 2005.11a
• /
• pp.283-283
• /
• 2005

• Journal of English Language & Literature
• /
• v.60 no.2
• /
• pp.395-400
• /
• 2014

• Journal of Distribution Science
• /
• v.2 no.1
• /
• pp.99-124
• /
• 2004

본 연구는 교수 및 학습효과를 증대시키기 위하여 실제 상황에서 일어나고 있는 사회현상들에 대한 보도형태의 자료인 신문기사를 비롯한 각종 매체 자료를 활용하여 사회 제 현상이 현재 학습과정의 교과과정에는 어떻게 연관되며 경영적 이론이 실제 어떻게 적용되는가를 학습함으로써 교과목에 대한 관심도의 증대와 학습심화의 필요성을 증대시키는데 그 목적이 있다. 이러한 목적을 달성하기 위하여 최근의 선별된 자료를 중심으로 각종 경제경영 관련주제의 주요 기사 내용을 숙지하고 주요 기사 내용들의 배경과 문제점을 파악하며 여러 관점에서 다양한 현상의 원인과 배경에 대한 접근으로 사회과학적인 현상을 습득하는 사고방법을 훈련할 수 있다. 또한 다양한 사회 경제경영분야의 요구사항과 사회적인 욕구들을 주지하고 향후 학생들의 사회진출에 따라 사회현상을 이해하고 이를 통하여 보다 적극적인 자신감 고취와 현실사회에 대한 이해력을 증진시킨다.

• Proceedings of the Korean Society for Environmental Edudation Conference
• /
• 2002.12a
• /
• pp.86-94
• /
• 2002

• Korean Journal of Ecology and Environment
• /
• v.52 no.4
• /
• pp.394-402
• /
• 2019

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

• Korean Journal of Veterinary Research
• /
• v.33 no.2
• /
• pp.199-209
• /
• 1993

The effects of ${\alpha}_1$-adrenergic stimulation on membrane potential, intracellular sodium activity $(a_N{^i{_a}})$, and contractility were investigated in the isolated papillary muscle of euthyroid, hyperthyroid, and hypothyroid guinea pigs. Cardiac alterations in the thyroid state have been shown to induce marked changes in action potential characteristics, the most pronounced shortening of action potential duration by hyperthyroidism and an increase in duration by hypothyroidism. $10^{-5}M$ Phenylephrine produced a decrease in $(a_N{^i{_a}})$ in euthyroid and hypothyroid preparations, but an increase in $(a_N{^i{_a}})$ in hyperthyroid ones. The major findings were that phenylephrine produced a stronger positive inotropic effect(PIE) without initial negative inotropic effect(NIE) in hyperthyroid preparations, while phenylephrine produced markedly NIE in hypothyroid ones. The alterations in membrane potential, $(a_N{^i{_a}})$, and contractility were abolished by $3{\times}10^{-5}M$ prazosin in hypothyroidism. In hypothyroid ventricular muscle, the decrease in $(a_N{^i{_a}})$ caused by phenylephrine were not abolished or reduced by $10^{-5}M$ strophanthidin, $10^{-5}M$ TTX, $3{\times}10^{-4}M$ lidocaine, or $100^{-5}M$ verapamil. These results indicate that the ${\alpha}_1$-adrenoceptor-mediated decrease in $(a_N{^i{_a}})$ is not associated with a stimulation of the $Na^+$-$K^+$ pump, inhibition of the $Na^+$ or $Ca^+$ channel in hypothyroid ventricular muscle. $10^{-5}M$ Phenylephrine decreased $(a_N{^i{_a}})$ but increased $(a_N{^i{_a}})$ in the presence of a PKC activator phorbol dibutyrate$(PDB_u)$. In conclusion, it is suggested that the following sequence of events in response to phenyleplhane occur in guinea pig ventricular muscle. First, changes in thyroid state may contribute to the ventacular electrophysiological propeties or ion transport system. Second, the adrenoceptor-mediated initial transient NIE may be associated with the decrease in $(a_N{^i{_a}})$ by PKC activation.

• Korean Journal of Environmental Biology
• /
• v.36 no.4
• /
• pp.638-646
• /
• 2018

The purpose of this study was to investigate the long-term habitat morphological alteration resulting from a large dam and weirs in the Tamjin River. To achieve this, we carried out a hydrograph analysis and a substrate size distribution analysis. We also estimated the channel width, bar area and vegetation encroachment using aerial photographs taken before and after the construction of the dam and weirs. The result of the hydrological analysis showed that flooding downstream was greatly reduced with small peaks occurrence after the dam construction. Interestingly, normal hydrographs in the main channel appeared just after tributary conjunction. There was a similar pattern in the substrate size analysis. Despite coarsened substrate just downstream of the dam site, more sand appeared again after introduction of the tributary. However, there was an increase in the bar area downstream of the dam's channels with most bars covered with vegetation. The channel width increased at the upper area of weirs through impoundment, but decreased downstream because of vegetation encroachment. This study indicate that unregulated tributary plays an important role in restoring hydro-physical habitat conditions in mainstream channels below a large dam. However, numerous weirs could be a causal factor to accelerate habitat deterioration in the dam downstream channels.