• 제목/요약/키워드: NH3-N

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Effect of Cassava Hay and Rice Bran Oil Supplementation on Rumen Fermentation, Milk Yield and Milk Composition in Lactating Dairy Cows

  • Lunsin, R.;Wanapat, Metha;Rowlinson, P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권10호
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    • pp.1364-1373
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    • 2012
  • Four crossbred (75% Holstein Friesian) lactating dairy cows, with an average live weight of $418{\pm}5$ kg and $36{\pm}10$ d in milk were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design to evaluate the effects of cassava hay (CH) and rice bran oil (RBO) on feed intake, nutrient digestibility, ruminal fermentation, milk yield, and milk composition. Factor A was non-supplementation or supplementation with CH in the concentrate. Factor B was supplementation with RBO at 0% or 4% in the concentrate mixture. The four dietary treatments were (T1) control (Concentrate with non-CH plus 0% RBO; C), (T2) Concentrate with CH plus 0% RBO (CH), (T3) Concentrate with non-CH plus 4% RBO (RBO), and (T4) Concentrate with CH plus 4% RBO (CHRBO). The cows were offered concentrate, at a ratio of concentrate to milk production of 1:2, and urea-lime treated rice straw was fed ad libitum. Urea-lime treated rice straw involved 2.5 g urea and 2.5 g $Ca(OH)_2$ (purchased as hydrated lime) in 100 ml water, the relevant volume of solution was sprayed onto a 100 g air-dry (91% DM) straw, and then covering the stack with a plastic sheet for a minimum of 10 d before feeding directly to animals. The CH based concentrate resulted in significantly higher roughage intake and total DM intake expressed as a percentage of BW (p<0.05). Ruminal pH, $NH_3$-N, BUN and total VFA did not differ among treatments, while RBO supplementation increased propionate, but decreased acetate concentration (p<0.05). Furthermore, the population of total ruminal bacteria was significantly lower on the RBO diet (p<0.05). In contrast, the total ruminal bacteria and cellulolytic bacteria on the CH diet were higher than on the other treatments. Supplementation with CH increased (p<0.05) F. succinogens and R. flavefaciens populations, whereas the populations of B. fibrisolvens and M. elsdenii were increased on the RBO diet. In addition, supplementation with CH and RBO had no effect on milk production and composition in dairy cows, while fatty acid composition of milk was influenced by RBO supplementation, and resulted in significantly lower (p<0.05) concentrations of both short-chain and medium-chain FA, and increased (p<0.05) the proportion of long-chain FA in milk fat, as well as significantly increased cis-9, trans-11 CLA and total CLA. In conclusion, RBO or CH exhibited specific effects on DMI, rumen fermentation, microbial population, milk yield and composition in lactating dairy cows, which were not interactions between CH and RBO in the diets. Feeding lactating dairy cows with RBO could improve fatty acid in milk fat by increasing cis-9, trans-11 CLA.

야제 (夜啼)의 한의학 치료에 대한 최신 중의학 임상 연구 동향 -2000년대 이후 발표된 임상 연구 논문을 중심으로- (Review of Clinical Research Literatures on Effect of Traditional Chinese Medicine for Pediatric Night Crying)

  • 김상민;이진용;이선행;도태윤
    • 대한한방소아과학회지
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    • 제32권3호
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    • pp.100-118
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    • 2018
  • Objectives The purpose of this study is to analyze some of the TCM (traditional Chinese medicine) clinical research literatures about pediatric night crying in order to learn clinical application of Korean medicine treatment for pediatric night crying. Methods We searched clinical trial literatures about TCM treatment of pediatric night crying from the CNKI (China National Knowledge Infrastructure) (January 2000 to June 2018). We analyzed the literature in regards to the treatment methods and the results. Results Among the 459 searched studies, 13 randomized controlled trials and 41 case studies were selected and analyzed. In most of the studies, the effectiveness of TCM in treating patients was significantly high, so we confirmed the effectiveness of TCM on pediatric night crying. The most commonly used pattern differentiations (辨證) were Spirit damage due to fright and fear (驚恐傷神), Spleen deficiency and cold (脾虛寒) and Heart fire heat (心火熱). Methods of treatment include herbal medicines (internal medicine and external application), massage therapy (Tuina methods), acupuncture and other treatments (bloodletting). The most commonly used herb medicines were Cicadidae Periostracum (蟬?), Poria Cocos (茯?), Glycyrrhizae Radix (甘草), Uncariae Ramulus et Uncus (釣鉤藤), Junci Medulla (燈心草), Fossilia Ossis Mastodi (龍骨), Atractylodis Rhizoma Alba (白朮), Cinnabaris (朱砂), and Coptidis Rhizoma (黃連). The most commonly used massage methods were Clearing Liver Channel (淸肝經), Clearing Heart Channel (淸心經), Kneading $Xi{\check{a}}oti{\bar{a}}nx{\bar{i}}n$ (?小天心), Supplementing Spleen Channel (補脾經), Clearing $Ti{\bar{a}}nh{\acute{e}}shu{\check{i}}$ (淸天河水), and Rubbing Abdomen (摩腹). Conclusions Based on the results of clinical studies from China, the use of Korean medicine for the treatment of pediatric night crying has been shown to be effective in relieving symptoms. Based on the results of this study, it is possible to widen the scope of Korean medicine by additionally reviewing clinical and experimental studies on pediatric night crying.

Evaluation of Coarsely Ground Wheat as a Replacement for Ground Corn in the Diets of Lactating Dairy Cows

  • Guo, Y.Q.;Zou, Y.;Cao, Z.J.;Xu, X.F.;Yang, Z.S.;Li, Shengli
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권7호
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    • pp.961-970
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    • 2013
  • Eight multiparous Holstein cows ($569{\pm}47$ kg of BW; $84{\pm}17$ DIM) were used to evaluate the effects of different levels of coarsely ground wheat (CGW) as replacements for ground corn (GC) in diets on feed intake and digestion, ruminal fermentation, lactation performance, and plasma metabolites profiles in dairy cows. The cows were settled in a replicated $4{\times}4$ Latin square design with 3-wk treatment periods; four cows in one of the replicates were fitted with rumen cannulas. The four diets contained 0, 9.6, 19.2, and 28.8% CGW and 27.9, 19.2, 9.6, and 0% GC on dry matter (DM) basis, respectively. Increasing dietary levels of CGW, daily DM intake tended to increase quadratically (p = 0.07); however, apparent digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly decreased (p<0.01) in cows fed the 28.8% CGW diets. Ruminal pH remained in the normal physiological range for all dietary treatments at all times, except for the 28.8% CGW diets at 6 h after feeding; moreover, increasing dietary levels of CGW, the daily mean ruminal pH decreased linearly (p = 0.01). Increasing the dietary levels of CGW resulted in a linear increase in ruminal propionate (p<0.01) and ammonia nitrogen ($NH_3$-N) (p = 0.06) concentration, while ruminal acetate: propionate decreased linearly (p = 0.03) in cows fed the 28.8% CGW diets. Milk production was not affected by diets; however, percentage and yield of milk fat decreased linearly (p = 0.02) when the level of CGW was increased. With increasing levels of dietary CGW, concentrations of plasma beta-hydroxybutyric acid (BHBA) (p = 0.07) and cholesterol (p<0.01) decreased linearly, whereas plasma glucose (p = 0.08), insulin (p = 0.02) and urea nitrogen (p = 0.02) increased linearly at 6 h after the morning feeding. Our results indicate that CGW is a suitable substitute for GC in the diets of dairy cows and that it may be included up to a level of 19.2% of DM without adverse effects on feed intake and digestion, ruminal fermentation, lactation performance, and plasma metabolites if the cows are fed fiber-sufficient diets.

Shrub coverage alters the rumen bacterial community of yaks (Bos grunniens) grazing in alpine meadows

  • Yang, Chuntao;Tsedan, Guru;Liu, Yang;Hou, Fujiang
    • Journal of Animal Science and Technology
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    • 제62권4호
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    • pp.504-520
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    • 2020
  • Proliferation of shrubs at the expense of native forage in pastures has been associated with large changes in dry-matter intake and dietary components for grazing ruminants. These changes can also affect the animals' physiology and metabolism. However, little information is available concerning the effect of pastoral-shrub grazing on the rumen bacterial community. To explore rumen bacteria composition in grazing yaks and the response of rumen bacteria to increasing shrub coverage in alpine meadows, 48 yak steers were randomly assigned to four pastures with shrub coverage of 0%, 5.4%, 11.3%, and 20.1% (referred as control, low, middle, and high, respectively), and ruminal fluid was collected from four yaks from each pasture group after 85 days. Rumen fermentation products were measured and microbiota composition determined using Ion S5™ XL sequencing of the 16S rRNA gene. Principal coordinates analysis (PCoA) and similarity analysis indicated that the degree of shrub coverage correlated with altered rumen bacterial composition of yaks grazing in alpine shrub meadows. At the phyla level, the relative abundance of Firmicutes in rumen increased with increasing shrub coverage, whereas the proportions of Bacteroidetes, Cyanobacteria and Verrucomicrobia decreased. Yaks grazing in the high shrub-coverage pasture had decreased species of the genus Prevotellaceae UCG-001, Lachnospiraceae XPB1014 group, Lachnospiraceae AC2044 group, Lachnospiraceae FCS020 group and Fretibacterium, but increased species of Christensenellaceae R-7 group, Ruminococcaceae NK4A214 group, Ruminococcus 1, Ruminococcaceae UCG-002, Ruminococcaceae UCG-005 and Lachnospiraceae UCG-008. These variations can enhance the animals' utilization efficiencies of cellulose and hemicellulose from native forage. Meanwhile, yaks grazed in the high shrub-coverage pasture had increased concentrations of ammonia nitrogen (NH3-N) and branched-chain volatile fatty acids (isobutyrate and isovalerate) in rumen compared with yaks grazing in the pasture without shrubs. These results indicate that yaks grazing in a high shrub-coverage pasture may have improved dietary energy utilization and enhanced resistance to cold stress during the winter. Our findings provide evidence for the influence of shrub coverage on the rumen bacterial community of yaks grazing in alpine meadows as well as insights into the sustainable production of grazing yaks on lands with increasing shrub coverage on the Qinghai-Tibet Plateau.

돈분액비 시용법이 논토양의 이화학적 특성과 벼 생육 및 수확량에 미치는 영향 (Effects of Liquid Pig Manuare Application Method on the Chemical Properties of Paddy Soil and Growth of Paddy Rice)

  • 안창현;김우식;박지성;안인
    • 한국토양비료학회지
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    • 제45권6호
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    • pp.1043-1048
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    • 2012
  • 돈분액비의 시용법이 벼재배시 토양과 생육에 미치는 영향을 비교하고자 미사질양토 논에서 각각의 시용법에 따라 돈분액비를 논토양에 시용하여 토양의 이화학적 특성과 벼 생육 및 수량을 조사하였다. 사용된 돈분액비의 유효성분 함량은 질소 0.44%, 인산 0.07%, 가리 0.14%였으며, 벼 표준시비량에 준하여 10a당 6.8톤을 기비와 추비로 나누어 시비하였다. 액비 시비 후 토양의 이화학성은 처리구간에 큰 차이가 없었다. 질소 함량은 관수혼입 추비구에서 다소 높았으나 균일성이 떨어지고 혼입부에 벼의 도장이 발생하였다. 액비 시비 후 경시적인 암모니아 가스발생은 살포량이 많을수록 높았으며 살포 당일에 가장 높았고, 써레질 후에는 거의 발생하지 않았다. 벼의 수량은 담수 기비구보다 건답 기비구에서 증수되었으나 처리구간에 큰 차이는 없었으며, 제트분무 추비구에서는 유수형성기에 과도한 질소공급으로 영양생장을 지속하여 등숙율이 약 20% 저하되었고 식물체에 물리적 피해도 나타났다. 가장 적은 수확량을 보인 담수 기비+제트분무 추비구와 비교하여 건답 기비+관수혼입 추비구에서 9.6% 증수되었다.

산화촉매를 이용한 Fast SCR에서의 SCR 촉매 저감 연구 (A Study for SCR Catalyst Reduction in Fast SCR Using Oxidation Catalyst)

  • 이재옥;이대훈;송영훈;오동규;서정욱
    • 공업화학
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    • 제24권3호
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    • pp.333-336
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    • 2013
  • 산업현장에서 배출되는 저온($150{\sim}250^{\circ}C$)의 배기가스에 포함된 질소산화물($NO_x$)을 제거하기 위한 공정으로써 산화 촉매와 암모니아 SCR 공정을 복합시킨 fast SCR 탈질공정에 대한 실험적인 연구가 수행되었다. Fast SCR 탈질공정을 위해서는 산화촉매를 이용 NO를 $NO_2$로 전환하는 것이 요구된다. 산화촉매 부피(Oxidation Catalyst Volume, OCV)에 따른 NO 전환율을 실험적으로 확인하였다. OCV 563000 h, 375000 h, 281000 h 각 경우에 NO 전환율은 37% : 45% : 51%이었다. 온도에 따른 fast SCR 탈질효율은 $NO_2/NO_x$ 비율 45%일 때 가장 높았다. $NO_2/NO_x$ 비율 45%, 온도 $200{\sim}250^{\circ}C$ 및 공간속도 $10000{\sim}30000h^{-1}$에서 standard SCR과 fast SCR 탈질효율을 비교한 결과, standard SCR은 공간속도 에 따라 온도별로 차이를 보였으나 fast SCR은 차이를 보이지 않았다. Fast SCR 반응을 고려한 탈질운전에서 공간속도 $10000h^{-1}$에서의 촉매부피와 비교하여 공간속도 $30000h^{-1}$으로 운전할 경우, NH3 SCR 촉매부피를 50% 이상 줄일 수 있음을 알 수 있었다.

논 토양성분(土壤成分)의 용탈(溶脫)에 관(關)한 연구(硏究) -III. 논 토양(土壤) 화학성분(化學成分)의 용탈(溶脫)에 미치는 볏짚의 영향(影響) (Studies on the Leaching of the Constituents in Paddy Soil -III. Effects of Rice Straw on the Leaching of the Constituents in Paddy Soil)

  • 김광식;김용웅
    • 한국토양비료학회지
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    • 제16권4호
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    • pp.311-317
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    • 1983
  • 논토양성분(土壤成分)의 용탈(溶脫)에 미치는 볏짚의 영향(影響)을 구명(究明)하기 위하여 논토양(土壤)에 볏짚을 시용(施用)하고 투수조건하(透水條件下)에서 수도(水稻)를 재배(栽培)하면서 침투수중(浸透水中) 용탈성분(溶脫成分)을 분석(分析) 검토(檢討)한 결과(結果)는 다음과 같다. 석회(石灰)와 고토(苦土)의 용탈(溶脫)은 초기(初期)에는 볏짚시용(施用)의 영향(影響)을 받았고 후기(後期)에는 수도근(水稻根)의 영향(影響)을 받았으며 철(鐵)과 망간의 초기용탈(初期溶脫)은 볏짚시용(施用)에 의(依)한 토양환원(土壤還元) 발달(發達)의 영향(影響)을 받았으나 후기(後期)에는 수도근(水稻根)의 영향(影響)을 크게 받아 증대(增大)되었다. 칼리, $NH_4-N$, 염소(鹽素) 등(等)의 용탈(溶脫)은 볏짚시용(施用)의 영향(影響)이 크지 않았으며 $CO_2$의 용탈(溶脫)은 볏짚의 영향(影響)을 받아 증대(增大)되고 동시(同時)에 수도근(水稻根)의 영향(影響)이 컸다. 침투수중(浸透水中) 용탈(溶脫)되는 양(陽)이온과 음(陰)이온의 양(量)은 대체로 같은 당량비(當量比)였으며 양(陽)이온은 석회(石灰)와 고토(苦土), 철(鐵), 음(陰)이온은 $HCO_3{^-}$$SO_4{^{-2}}$이 중요(重要)한 용탈성분(溶脫成分)이었다.

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고효율 혐기성반응조 및 호기성여상 조합시스템에 의한 질소·유기물 동시 제거 (Simultaneous Carbon and Nitrogen Removal Using an Integrated System of High-Rate Anaerobic Reactor and Aerobic Biofilter)

  • 성문성;장덕;서성철;정보림
    • 상하수도학회지
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    • 제13권2호
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    • pp.55-65
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    • 1999
  • AF(anaerobic filter)/BAF(biological aerated filter) system and UASB(upflow anaerobic sludge blanket)/BAF system, of which system effluents were recirculated to the anaerobic reactors in each system, were operated in order to investigate the performance in simultaneous removal of organics and nitrogen in high-strength dairy wastewater. Advanced anaerobic treatment processes of AF and UASB were evaluated on applicability as pre-denitrification reactors, and BAF was also evaluated on the performance in oxidizing the remaining organics and ammonia nitrogen. At system HRTs of 4.0 to 4.5 days and recirculation ratios of one to three, the AF/BAF system could achieve more than 99% of organics removals and 64 to 78% of total nitrogen removals depending upon the recirculation ratio. Although the UASB/BAF system also showed more than 99% of organics removals, total nitrogen removals in the UASB/BAF system were 53 to 66% which are lower than those in the AF/BAF system at the corresponding recirculation ratios. Optimum recirculation ratios considering simultaneous removal of organics and nitrogen and cost-effectiveness, were in the range of two to three. The upflow AF packed with crossflow module media, as a primary treatment of the anaerobic reactor/BAF system, showed better performances in denitrification, SS removals, and gas production than the UASB. Higher loading rate of suspended solids from the UASB increased the backwashing times in the following BAF. Especially, at a recirculation ratio of three in the UASB/BAF system, the increase in head loss due to clogging in the BAF caused frequent backwashing, at least once d day. The BAF showed the high nitrification efficiency of average 99.2% and organics removals more than 90% at organics loading rate less than $1.4KgCOD/m^3/d$ and $COD/NH_3-N$ ratio less than 6.4. It was proved that the simplified anaerobic reactor/BAF system could maximize the organics removal and achieve high nitrogen removal efficiencies through recirculation of system effluents to the anaerobic reactor. The AF/BAF system can, especially, be a cost effective and competitive alternative for the simultaneous removal of organics ana nitrogen from wastewaters.

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Effects of Dietary Supplementation with Hainanmycin on Protein Degradation and Populations of Ammonia-producing Bacteria In vitro

  • Wang, Z.B.;Xin, H.S.;Wang, M.J.;Li, Z.Y.;Qu, Y.L.;Miao, S.J.;Zhang, Y.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권5호
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    • pp.668-674
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    • 2013
  • An in vitro fermentation was conducted to determine the effects of hainanmycin on protein degradation and populations of ammonia-producing bacteria. The substrates (DM basis) for in vitro fermentation consisted of alfalfa hay (31.7%), Chinese wild rye grass hay (28.3%), ground corn grain (24.5%), soybean meal (15.5%) with a forage: concentrate of 60:40. Treatments were the control (no additive) and hainanmycin supplemented at 0.1 (H0.1), 1 (H1), 10 (H10), and 100 mg/kg (H100) of the substrates. After 24 h of fermentation, the highest addition level of hainanmycin decreased total VFA concentration and increased the final pH. The high addition level of hainanmycin (H1, H10, and H100) reduced (p<0.05) branched-chain VFA concentration, the molar proportion of acetate and butyrate, and ratio of acetate to propionate; and increased the molar proportion of propionate, except that for H1 the in molar proportion of acetate and isobutyrate was not changed (p>0.05). After 24 h of fermentation, H10 and H100 increased (p<0.05) concentrations of peptide nitrogen and AA nitrogen and proteinase activity, and decreased (p<0.05) $NH_3$-N concentration and deaminase activity compared with control. Peptidase activitives were not affected by hainanmycin. Hainanmycin supplementation only inhibited the growth of Butyrivibrio fibrisolvens, which is one of the species of low deaminative activity. Hainanmycin supplementation also decreased (p<0.05) relative population sizes of hyper-ammonia-producing species, except for H0.1 on Clostridium aminophilum. It was concluded that dietary supplementation with hainanmycin could improve ruminal fermentation and modify protein degradation by changing population size of ammonia-producing bacteria in vitro; and the addition level of 10 mg/kg appeared to achieve the best results.

여러 가지 첨가제가 맥주박과 베지밀박의 부패방지에 미치는 영향 (Effects of Various Additives for Spoilage Prevention on Brewers grain and Soymilk by-product)

  • 이현준;김현섭;전병순;김상우;기광석;조광근;조재순;이홍구;최윤재
    • 유기물자원화
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    • 제10권3호
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    • pp.74-84
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    • 2002
  • 고수분 유기성 물질인 맥주박, 베지밀박 등의 농산가공부산물들은 상온에서 변패성이 강하여 가축의 사료로 이용하기위해서는 보관성증진을 위한 첨가제의 처리가 중요하다. 본 연구에서는 맥주박과 베지밀박을 소금, 유기산, 발효미생물 및 메치오닌수산화물(MHA)을 처리하여 고온다습한 7월중순경에 상온에서 20일간 방치한 후 보관일수에 따른 시료의 표면연화현상과 곰팡이 발생양상, 시료내부 pH, 암모니아태질소 함량변화를 조사하여 각 첨가제의 시료에 대한 변패방지효과를 비교한 결과, MHA 5%이상 처리구에서 특별한 효과가 있는 것으로 나타내었다.

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