• Title/Summary/Keyword: ND4

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Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions (염분, 저온 및 가뭄 스트레스 조건에서 벼 ND0001 oscpk11 돌연변이체의 OsCPK11 발현 분석)

  • Kim, Hyeon-Mi;Kim, Sung-Ha
    • Journal of Life Science
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    • v.31 no.2
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    • pp.115-125
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    • 2021
  • Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs' functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4℃ and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.

Temperature Changes in Dentin upon Pulsed Nd:YAG Lasing Distance (Pulsed Nd:YAG 레이저 조사거리에 따른 상아질의 온도변화)

  • Jae-Hyung Kim;Woo-Cheon Kee
    • Journal of Oral Medicine and Pain
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    • v.20 no.2
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    • pp.327-334
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    • 1995
  • In order to observe the influence of pulsed Nd:YAG laser at its out-of-contact with dentin on tooth temperature, we have applied pulsed Nd:YAG laser to 2mm thick dentin sample at a point of contact and from a distance of 1mm, 2mm, 3mm and 4mm with an energy of 0.3W, 0.5W, 0.8W, 1.5W and 2.0W. They were exposed to periods of 3 seconds, 6 seconds, 9 seconds and 15 seconds respectively and measured temperature changes. The results as follows : 1. When the time ad intensity of power were constant, the temperature changes on dentin of tooth depended on the distance. The temperature increased when the laser intensity increased bu two other conditions were contact. 2. At the point of contact, the temperature has risen over $5^{\circ}C$ regrdless of intensity of the power or the time. However, there was $5^{\circ}C$ fluctuation with 0.3 W for 3 seconds treatment. 3. The temperature change was less than $5^{\circ}C$ thermal change at the distance of 1mm and 2mm respectively when lased for 3 seconds, 6 seconds, 12 seconds and 15 seconds with 0.3 W. Similar results were observed at 3 and 6 seconds treatment with 0.5 W and at 3 seconds treatment with 0.8 and 1.0W respectively. 4. It showed less than 5(C thermal change when lased for 3 seconds, 6 seconds, 9 seconds, 12 seconds and 15 seconds with 0.3W at the distance of 3mm and 4mm. The same results were seen in 3 seconds, 6 seconds and 9 seconds treatment with 0.5W and in 3 seconds with 0.8W and 1.0W respectively. As we have seen the above, the results has indicated that pulsed Nd:YAG lasing at its off contact on dentin of 2mm thickness will not cause irreversible changes if lasing intensity, lasing distance and lasing time are appropriate.

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ITO, PR, 격벽 재료의 레이저 직접 미세가공

  • Lee, Cheon;Lee, Gyung-Chul;Ahn, Min-Young;Lee, Hong-Gyu
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.80-80
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    • 1999
  • 플라즈마 디스플레이 패널(PDP)의 공정을 간단히 하기 위하여 포토레지스트, ITO, 격벽재료를 Ar+ laser(λ-514 nm, CW)와 Nd:YAG laser(λ=532, 266nm, pulse)로 직접 패터닝 하였다. 레이저에 의한 포토레지스트의 패턴결과, 아르곤 이온 레이저의 포토레지스트 가공의 반응 메카니즘은 레이저 빔의 열에 의한 시료 표면의 국부적인 온도상승에 의한 용융작용이며, 그 결과 식각 후 형성된 패턴의 단면 모양도 레이저빔의 profile과 같은 가우시안 형태를 나타낸다. Nd:YAG 레이저의 4고조파(532nm)를 이용한 경우 200$\mu\textrm{m}$/sce의 주사속도에서 포토레지스트를 패턴하기 위한 임계에너지(threshold energy fluence) 값은 25J/cm2이며, 약 40J/cm2의 에너지 밀도에서 하부기판의 손상이 발생하기 시작하였다. 글미 1은 Nd:YAG 레이저 4고조파를 이용하여 포토레지스트를 식각한 경우 SEM 표면사진(위)과 단차특정기에 의한 단면형상(아래)이다. ITO 막의 레이저에 의한 직접 패턴 결과, ITO 막은 레이저 펄스에 의한 급속 가열 및 증발에 의한 메커니즘으로 식각이 이루어지며, 레이저 파장에 따른 광흡수 정도의 차이에 의해 2고조파 (532nm)에서 ITO 막의 가공 품질이 4고조파(266nm)에 비해 우수하며 패턴의 폭도 출력에 따라 제어가 용이하였다. 그림 2는 Nd:YAG 레이저 2고조파를 이용하여 ITO를 식각한 경우 SEM표면 사진(위)과 단차측정기에 의한 단면형상(아래)이다. 격벽 재료의 레이저에 의한 직접 패턴 결과, Ar+ 레이저(514nm)는 출력 밀도 32NW/cm2에서 격벽을 유리 기판의 경계면까지 식각하였다. Nd:YAG 레이저(532nm)는 laser fluence가 6.5mJ/cm2에서 격벽을 식각하기 시작하였으며, 19.5J/cm2에서 유리기판의 rudraus(격벽 두께 130$\mu\textrm{m}$)까지 식각하였다.

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THE CLINICAL STUDY OF THE EFFECT OF OCCLUSAL PLANES ON THE STOMATOGNATHIC SYSTEM I. THE CONSIDERATION OF OCCLUSAL PLANES USING PANORAMIC VIEWS (교합평면이 악구강계에 미치는 영향에 대한 임상적 연구 I. Panoramic View를 이용한 교합평면의 검토)

  • Jo, Byung-Woan;Ahn, Sang-Hun;Kim, Jong-Pil
    • The Journal of Korean Academy of Prosthodontics
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    • v.33 no.4
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    • pp.705-717
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    • 1995
  • In clinical prosthetic dentistry the occlusal planes were represented to the dental arch form sagittally and composed of the occlusion morphologically. From now on the determinations of the occlusal planes were done through the facebow transfer and the cephalometric radiography, but they were not exact method for the diagnosis of the stomagnathic function. The purpose of this study is to examine the sagittal characteristics of occlusal planes using panoramic view between the normal subjects and the subjects with stomatognathic dysfunction. 60 normal subjects and 16 subjects with stomatognathic dysfunction were slectec for this investigation. We measured the inclination of occlusal planes to Frankfurt Horizontal plane. The occlusal planes were divided into 4 groups : 1) The plane between the midpoint of upper central incisor and the mesiobuccal cusp of upper 1st molar, 2) The plane between the midpoint of upper central incisor and the mesiobuccal cusp of upper 2nd molar, 3) The plane between the midpoint of upper central incisor and the distobuccal cusp of upper 2nd molar, 4) The plane between the mesiobuccal cusp of 1st molar and the distobuccal cusp of 2nd molar. The results were as follows : 1) The occlusal planes could be determined very easy by using panoramic view, 2) Among the angles between the occlusal planes and the FH plane, the angles related with the 2nd molar were more steep than those of the 1st molar. The angle between the 1st molar and the 2nd molar is the most steep in the other angles. 3) In the angles between occlusal planes and FH plane, the subjects with stomatognathic dysfunction were more significant steep(p<0.05) than the normal subjects.

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The Content and Risk Assessment of Heavy Metals in Herbal Medicines used for Food and Drug (식약공용 농·임산물의 유해중금속 분석 및 위해도 평가)

  • Cho, In-Soon;Kim, Su-Jin;Park, Ae-Suk;Kim, Jin-Ah;Jang, Jung-Im;Lee, Sung-Deuk;Yu, In-Sil;Shin, Yong-Seung
    • Journal of Food Hygiene and Safety
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    • v.35 no.4
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    • pp.354-364
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    • 2020
  • For this paper we analyzed heavy metals including lead, cadmium, arsenic and mercury, and conducted risk assessment on 171 types of herbal medicines used as foods and drugs distributed in Seoul's Yangnyeongsi and Kyungdong (Gyeongdong) herbal medicine markets. The concentrations of the heavy metals were measured by the ICP-MS and a mercury analyzer. The detection ranges of the lead, cadmium, arsenic and mercury were found to be ND-4.719 mg/kg, ND-1.019 mg/kg, 0.002-8.696 mg/kg and ND-0.058 mg/kg, respectively. Artemisiae Capillaris Herba exceeded the standards of the MFDS (Ministry of Food and Drug Safety) for cadmium items, and Actinidiae Ramulus et Fulium et Fructus Vermicultus exceeded the standards for arsenic items. The non-carcinogenic risk assessment showed that there were no items exceeding 1 and that they were safe. The risk of carcinogenicity to lead was below 10-6 in all items and was evaluated as safe. The risk of carcinogenicity of arsenic was almost all in the range of 10-4 to 10-6. All %PTWI are considered to be safe below 100.

A Canine Model of Tracheal Stenosis Using Nd-YAG Laser (Nd-YAG laser를 이용한 기관협착 동물모델의 개발)

  • Kim, Jhin-Gook;Suh, Gee-Young;Chung, Man-Pyo;Kwon, O-Jung;Suh, Soo-Won;Kim, Ho-Joong
    • Tuberculosis and Respiratory Diseases
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    • v.52 no.1
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    • pp.54-61
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    • 2002
  • Background: Tracheal stenosis is an urgent but uncommon disease. Therefore, primary care clinicians have limited clinical experience. Animal models of a tracheal stenosis can be used conveniently for the learning, teaching, and developing new diagnostic and therapeutic modalities for tracheal stenosis. Recently, a canine model of a tracheal stenosis was developed using a Nd-YAG laser. To describe the methods and results of developed animal model, we performed this study. Methods : Six Mongrel dogs were generally anesthetized and the anterior 180 degree of tracheal cartilage of the animal was photo-coagulated using a Nd-YAG laser. The animals were bronchoscopically evaluated every week for 4 weeks and a pathologic evaluation was also made. Results : Two weeks after the laser coagulation, the trachea began to stenose and the stenosis progressed through 4 weeks. All animals suffered from shortness of breath, wheezing, and weight loss in the 3 weeks after the laser treatment, and two died of respiratory failure just before the fourth week. The gross pathologic findings showed the loss of cartilage and a dense fibrosis, which resulted in a fibrous stricture of the trachea. Microscopy also showed that the fibrous granulation tissue replaced destroyed cartilage. Conclusion : The canine model can assist in the understanding and development of new diagnostic and therapeutic modalities for tracheal stenosis.

A Study on the Coloration of the $Nd_2O_3$ Containing Glass by X-ray Irradiation ($Nd_2O_3$ 함유 유리의 X-선조사에 따른 변색에 관한 연구)

  • 박용원;강원호
    • Journal of the Korean Ceramic Society
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    • v.21 no.4
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    • pp.373-381
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    • 1984
  • The coloration of the $Nd_2O_3$ contained $R_2O-BaO-SrO-ZrO_2-SiO_2$ glass added the various amount of $CeO_2$ $MnO_2$, $Fe_2O_3$ and $As_2O_3$ alone or together by the irradiation of X-ray irradiation,. The glasses added $CeO_2$ in proportion to amount were more effective on preventing coloration by X-ray irradiation but the addition of $MnO_2$ produced different color according to the amount of addition. The addition of the $Fe_2O_3$, $TiO_2$ and $As_2O_3$ did not give much effects to the transmission changes of $Nd_2O_3$ contained glass by X-ray irradiation but the glass added $CeO_2$ , $Fe_2O_3$, $TiO_2$ together was most effective to prevent coloration and transmisson changes.

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A Study on the Welding Behavior of A3003 Aluminium Alloy Thin Sheet by Nd : YAG Laser Beam (박판 A3003 Al합금의 Nd : YAG 레이저빔 용접에 관한 연구)

  • 허인석;김병철;김도훈;김진수;이한용
    • Laser Solutions
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    • v.4 no.1
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    • pp.29-38
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    • 2001
  • This work was carried out to investigate the welding behavior of thin A3003 Al alloy sheets by Nd : YAG laser beam. Considering bead shape and mechanical properties, the laser pulse shapes selected were two kinds of 2-division and 3-division by varying power level and pulse duration. In order to obtain optimum conditions, the factorial design method and central composite design method were applied. Tensile test, optical microscopy, micro hardness test and TEM analysis were performed. Due to the annealing caused by thermal effect during laser welding, precipitates were coarsended. The HAZ was softened and failed during tensile test. The hardness of HAZ was lower than that of base metal, since the heat input relieved the work hardening effect and caused grain growth.

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Changes of maternal antibodies in chicks vaccinated to breeder against infectious bronchitis, infectious Bursal disease, and Newcastle disease virus (모계의 전염성기관지염, 전염성 F낭병 및 뉴캣슬병 백신투여에 따른 모체이행 항체의 변동)

  • 고원석;김태중;이정원;서이원;송희종;오언평
    • Korean Journal of Veterinary Service
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    • v.21 no.2
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    • pp.133-139
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    • 1998
  • Serum samples collected from 30 breeders and their progeny 30 chicks. The antibodies against infectious bronchitis(IB), infectious bursal disease (IBD) and Newcastle disease(ND) viruses were detected by ELISA using commercial ELISA kit. The breeders were vaccinated against IB, IBD and ND viruses according to general vaccination program. Geometric mean titers(GMT) of ELISA were monitored from 1-day old to 17-day old chicks and compared with breeder chickens. The GMT of ELISA to IB, IBD and ND were declined half level of the breeder antibody titer at 6-, 8- and 7-day old. And, the GMT of ELISA to IB, IBD and ND were declined than that of protective titer at 6-, 1-, and 4-day old. Thereafter, the GMT of ELISA was declined and disappeared according to ages of chicks. Taken together, this study led to conclusion that time-course of maternal antibody titers of chicks from vaccinated breeders, and this is very important data for vaccination to chicks.

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