• 제목/요약/키워드: ND4

검색결과 5,022건 처리시간 0.039초

한국산 연어의 미토콘드리아 NADH Dehydrogengse Subunit 3 영역의 클로닝 및 DNA 염기서열 분석 (Cloning and DNA Sequences Anaylsis of Mitochondrial NADH Dehydrogenase Subunit 3 from Korean Chum Salmon, Oncorhynchus keta)

  • 최윤실;이윤호;진덕희
    • 한국수산과학회지
    • /
    • 제36권2호
    • /
    • pp.94-99
    • /
    • 2003
  • Mitochondrial DNAs has been used frequently as genetic markers for the population genetic studies of salmonid fishes. Samples used in this experiment were chum salmons (Oncorhynchus keta) from Korea. We analyzed variation of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) among 4 individuals of the Korea population. Genomic DNA was extracted from the liver of the chum salmon samples. Then, the ND3 gene was amplified by polymerase chain reaction (PCR) including the 3' region of cytochrome oxidase III gene (COIII) and the 5` region of NADH dehydrogenase subunit 4L gene (ND4L). The size of the PCR product was 752 Up and the sequences showed some genetic variation among those four individuals. Genetic variations were observed in 7 sites as single nucleotide polymorphism (SNP). Within the open reading frame of the ND3 gene which encodes 116 amino acids, 5 nucleotide substitutions were found. Both transitional and transversional changes occurred more frequently with transitional changes. Comparison of these sequences with the others of a Japanese chum salmon in GenBank showed 5 sites of SNPs. This study provided the basic information of SNP in ND3 gene among Korean chum salmons and demonstrated the possible use of the SNP data as a genetic marker.

양식산 굴(Crassostrea gigas)의 생굴 및 가공소재용으로서 화학적 및 생물학적 위생 특성 (Chemical and Biological Properties on Sanitary of Cultured Oyster Crassostrea gigas Intended for Raw Consumption or Use in Seafood Products)

  • 박선영;이경돈;이정석;허민수;이태기;김진수
    • 한국수산과학회지
    • /
    • 제50권4호
    • /
    • pp.335-342
    • /
    • 2017
  • Oysters Crassostrea gigas are a globally popular shellfish for human consumption. As filter-feeding bivalve mollusks, oysters may harbor many microorganisms and chemicals that could pose potential human health risks. The objective of this study was to investigate the suitability of cultured oysters for raw consumption or use in seafood products by measuring concentrations of harmful microorganisms and chemicals in their flesh. Microbial concentrations in cultured oysters were found to be: $1.0{\times}10^2-6.0{\times}10^4CFU/g$ (viable cell counts), not detected $(ND)-5.4{\times}10^3CFU/g$ (coliform bacteria), $ND-1.3{\times}10^2CFU/g$ (E. coli), and $ND-4.6{\times}10^3CFU/g$ (Vibrio parahaemolyticus). Other pathogenic bacteria, including Enterohemorrhagic E. coli (EHEC), Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp., were not detected in any samples. Heavy metal concentrations of cultured oysters were ND-0.239 mg/kg (total mercury), ND-1.091 mg/kg (lead), ND-0.968 mg/kg (cadmium). The concentrations of benzo(a)pyrene ranged from $0.280-0.880{\mu}g/kg$. Paralytic shellfish poison ranged from ND-0.58 mg/kg, while diarrhetic shellfish poison was not detected. No radioactivity was detected. These results suggest that oysters intended for raw consumption or use in seafood products should be subjected to chemical and biological controls.

연분홍색의 절화용 스프레이국화 'Noble ND' 육성 (Breeding of a New Light Pink Spray-mum 'Noble ND' as Cut Flower)

  • 김현석;성전중;한윤열;최동진;우진하
    • 원예과학기술지
    • /
    • 제28권5호
    • /
    • pp.895-898
    • /
    • 2010
  • 절화용 스프레이국화 'Noble ND'는 백색의 아네모네 화형인 '모나리자'에 분홍색의 아네모네 화형인 '다크웰던'을 2004년 10월에 교배를 하여 선발한 계통으로부터 육성하였다. 2006년부터 2년간 촉성재배, 자연재배 및 억제재배 작형에서 특성검정을 실시하였는데, 'Noble ND'는 연분홍색의 아네모네 화형으로 10월 하순에 자연개화하는 중생 추국으로 자연재배 작형에서 본당 착화수는 9.2개, 꽃의 직경은 4.8cm이었다. 촉성재배 작형에서의 개화소요일수는 57일이었고, 본당 착화수는 7.4개였다. 억제재배 작형의 개화소요 일수는 48일이었다.

유전적 형질에 의한 북태평양 연어 (Oncorhynchus keta)의 계군 구분 (Genetic Identification of the North Pacific Chum Salmon (Oncorhynchus keta) Stocks)

  • 정웅식;이윤호;김수암;진덕희;성기백
    • 한국수산과학회지
    • /
    • 제36권6호
    • /
    • pp.578-585
    • /
    • 2003
  • The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.