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Characterization of Extended-Spectrum $\beta$-Lactamases (ESBL) Producing Klebsiella and Enterobacter Isolated from Sewerage Plant Drain Water at Kwang-An in Pusan (광안리 오수처리장에 분리된 Extended-Spectrum $\beta$-Lactamase (ESBL) Klebsiella와 Enterobacter의 유형)

  • 이훈구
    • Korean Journal of Microbiology
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    • v.37 no.4
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    • pp.277-283
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    • 2001
  • The emergence of extended spectrum beta-lactamase(ESBL) producing bacteria is causing very serious problems in Korea. Although there have been many reports about these bacteria isolated from patients and clinical specimens, there is no report of ESBL-producing organisms isolated from natural evironment in Korea. This is the first study on the ESBL producing bacteria out of the medical system in Korea. Twenty-six ESBL producing bacteria were isolated only from sewerage plant drain water at Kwang-an beach among the sampling collected sites including snakehead fish plants in Myungi, Aquaculture Engineering Lab. in Pukyong National University and two public-bathrooms in Pusan, Korea. ESBL producing bacteria were identified by double-disk synergy test, conjugation, isoelectric focusing values and PCR. The species of ESBL producing bacteria were Enterobacter cloacae(4 strains), E. sakazakii(8 strains), Klebsiella pneumoniae subsp. pneumoniae(8 strains) and K. pneumoniae subsp. ozaenae(6 strains). TEM and SHV specific PCR products were detected from all the ESBL strains produced TEM+SHV products on the PCR plates. The pI values of ESBL produced by Klebsiella pneumoniae subsp. pneumoniae, K. pneumoniae subsp. ozaenae, Enterobacter cloacae, and E. sakazakii were 5.9, 5.9+5.4; 5.9, $5.9+5.4;{\ge}8.5$, 8.0+5.4, and 8.0+5.4, respectively on the IEF. Seven strains of the isolates were transfered their genes to E. coli RG488 $Rif^r$ by conjugation.

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A Review Study on Adjustment of College Freshmen for Suggesting Adaptation Program (적응프로그램 제언을 위한 대학신입생 학교적응 관련 문헌분석)

  • Yoo, Kyung Hee;Kim, Jong Kyung
    • The Journal of the Korea Contents Association
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    • v.16 no.8
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    • pp.580-591
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    • 2016
  • The purpose of this study was to review articles and theses on adjustment of college freshmen from domestic researches conducted during the past 11 years, to identify overall research trends, and to suggest direction and strategies for college freshman's adjustment. The final 24 articles were selected from three databases, RISS, KISS, and NAL (National assembly library) using specific inclusion criteria. Twenty were correlation studies and four were experimental studies. In 2009, the first study on adjustment of college freshmen was published. Since then several more studies have been conducted to date. This means that people have recognized the importance of adjustment of college freshmen. Twenty three studies that are reviewed used Baker and Siryk's college adjustment tool. From reviewed correlation studies, mean score of college students' adjustment was 3.25. The related variables with adjustment of college freshmen revealed 20 items, especially self-esteem, self-efficacy, and stress showed important related concepts. The general characteristics related to adjustment of freshmen were outgoing character, living with family, aged students, satisfied with the area of study, and satisfied with school life. The findings of this study indicated that colleges when planning for the freshmen orientation program should focus on improving self-efficacy of students, stress management, mentor program, and freshmen adjustment program for each department of the school and college.

Comparison of Methods for Detection of Escherichia coli O157:H7 in Ground Beef and Radish Sprouts

  • Lee, Jae-Hoon;Hyeon, Ji-Yeon;Heo, Seok;Hwang, In-Gyun;Kwak, Hyo-Sun;Choi, In-Soo;Park, Chan-Kyu;Seo, Kun-Ho
    • Food Science of Animal Resources
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    • v.30 no.2
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    • pp.179-184
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    • 2010
  • Escherichia coli O157:H7 is a food-borne pathogen that causes bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). We compared three selective media and evaluated the performance of immunomagnetic separation (IMS) for the detection of low levels of E. coli O157:H7 in ground beef and radish sprouts with different levels of background flora. Bulk food samples (500 g for each trial) were artificially inoculated with nalidixic acid-resistant E. coli O157:H7 at the lowest dose that would generate 20 partial-positive samples of 25 g each. All samples were homogenized in mTSB (225 mL) and incubated overnight at $37^{\circ}C$. IMS was performed using the enriched mTSB samples (1 mL) along with conventional spreads plated onto three different selective media: Sorbitol MacConkey agar (SMAC), Sorbitol MacConkey agar with cefixime and tellulite (CT-SMAC), and Sorbitol MacConkey agar with nalidixic acid (NAL-SMAC) as the gold standard. Two suspicious colonies from each medium were selected and confirmed usinga serological test after transfer to tryptic soy broth with yeast extract (TSAYE). CT-SMAC was better than SMAC for detecting E. coli O157:H7 in all food types. Although there was no statistical difference in the number of positive samples when using IMS vs. non-IMS techniques, more positive samples were detected when IMS was used in both ground beef and radish sprouts. It appears that the improvement was more significant in radish sprouts, which had a higher level of background flora than ground beef. The results also suggest that the combination of CT-SMAC and IMS is sufficient to recover low levels of E. coli O157:H7 in high background flora food samples.

A Review of the Korean Nursing Research Literature with MBTI Personality and Nursing Students (국내 간호학생 성격관련 연구 문헌고찰 : MBTI를 중심으로)

  • Hong, Eun-Young
    • Journal of the Korea Convergence Society
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    • v.13 no.1
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    • pp.425-436
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    • 2022
  • The purpose of this study was to explore the trends of research on MBTI (Myers-Briggs Type Indicator) and nursing students, and to suggest directions for nursing education geared to the needs of the future. The literature was searched using the National Assembly Library, Korean Studies Information Service System, DBPIA, Korean Medical database and National Discovery for Science Library to identify studies including MBTI personalty among nursing students. This study selected a total of 22 precedent studies regarding the investigation of MBTI personalty and nursing students. All studies were quantitative study and 63.6% of them were nonexperimental cross-sectional study. The most frequently selected category of variables was cognitive-perceptual related variables includes self-efficacy, self-eseem, academic self efficacy and etc. The most frequent MBTI personality type of nursing students was ISTJ (12.3%) and ESTJ (11.5%) was the second. Based on the findings of this study, longitudinal reasearch is recommanded on MBTI personality type and nursing specialty choice. Implications for teaching and learning strategies, and for using the results of MBTI in nursing students' career guidence are discussed.

Recipe Standardization and Nutrient Analysis of 'Dong-rae Pajeon' (Local Food in Busan) (부산 향토음식 동래파전의 조리표준화 및 영양분석)

  • Kim, Sang-Ae;Shin, Eun-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.11
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    • pp.1472-1481
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    • 2007
  • The purposes of the study were to find refined taste of ancestor through historical research about traditional cooking method and ingredient for the purpose of enriching today#s dietary life and to hand down a particular style of regional dish and excellence of nutritional aspect by providing a standard recipe and nutrition analysis data on #Dong-rae Pajeon#. To collect data about traditional ingredients and cooking method, researcher interviewed seven local natives who have kept a traditional food costumes, visited four restaurants, and reviewed ten cookbooks. The interviewees recalled and demonstrated the cooking procedure. The standard recipe of #Dong-rae Pajeon# was created after three experimental cookings, based on the recipes of the natives, restaurants, and cookbooks. According to the natives# statements, #Dong-rae Pajeon# was a special dish that was offered to the king at #Samzi-nal# (March 3rd of the lunar calendar). It was also a seasonal (before cherry blooming time) and memorial service dish of the province#s high society. The main ingredients were small green onion, dropwort, beef, seafood (large clam, mussel, clam meat, oyster, shrimp, fresh water conch), waxy rice powder, non-wax rice powder, and sesame oil which were abundant in Busan and Kijang region. Energy per 100 g of #Dong-rae Pajeon# was 148 kcal. Protein, lipid, fiber, Ca, and Fe contents were 8.8 g, 2.0 g, 8.6 g, 57.7 mg, and 1.8 mg respectively. Contents of cystine, lysine, leucine, valine, isoleucine which are essential amino acids were high in #Dong-rae Pajeon#. Fatty acids contents are oleic acid (20.5%), linoleic acid (20.1%) and linolenic acid (10.4%) while P/M/S ratio was 0.73/0.67/1.