• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.52-58
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• 1991

Interspecific cross between Nicotiana trigonophylla and N. tabacum cv. BY4 is highly sterile because of abnormal ovule and embryo development. In vitro culture of excised N. trigonophylla ovules after polination by N. tabacum allows significant numbers of hybrid embryos to develop into mature plants. Total yield of seedlings and number of normal seedlings were produced following in vitro culture of individual fertilized ovules of N. trigonophylla X N. tabacum at four days post-pollination on B5 medium containing 6% sucrose. Hybrids were uniform in morphology and peroxidase isozyme composition and the majority were cytologically stable: flower characteristics were generally intermediate between those of the parents. All hybrids evaluated were self-sterile.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.123-129
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• 1993

Mesophyll protoplasts derived from young leaves of Nicotiana rustica and N. tabacum cv Burley 21 were fused with the aid of polyethylene glycol(PEG). Cytological examination of protoplasts after PEG treatment revealed 12.8 % heterokaryocytes. After 7 weeks culture, the hybrid calli showing greenish white with a compact appearance were selected in contrast to parental type calli tinged with white or green color. The somatic hybrid plants were verified by morphological, biochemical and cyclological analysis. A heterosis effect for plant vigor and height was observed but the shape of leaves and flower characteristics were intermediate between N. tabacum and N. rutstica. The isozyme banding patterns for peroxidase of somatic hybrid lines were compared with the parent species. A number of isozyme bands derived from both parental species were found in the hybrids. Somatic hybrid plants have been successfully backcrossed to the parental N. tabacum particularly with somatic hybrid plants as female parents. These hybrid plants yielded small seeds, only few which were germinable.

• KSBB Journal
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• v.5 no.2
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• pp.175-182
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• 1990

Protoplasts, isolated from leaf of N. tabacum NR-/SR+ and N. glottnosa were electrofused and divided with a plating efficiency of 30∼35% in AAPI 9M medium. Green callus lines were selected in protoplast-derived colonies on MSNO3 selection medium with 1.2mg/ml streptomycin sulfate on the basis of nitrate reductase proficiency and streptomycin resistance. Four putative hybrid plant lines regenerated from the green callus lines had intermediate morphology between that of parents with respect to floral shape, corolla length and ovate leaf blade. Zymograms of leaf peroxidase and esterase from these putative hybrid plant lines showed isozyme profiles derived from both parents and also, they exhibited additional and lost bands. Cytological analysis of two putative hybrid plant lines gave chromosome counts of 2n=66 in L22 and 2n=54 in L44 which were less than the expected number of N. tabacum(2n=48) and N. glutinosa(2n=24).

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.93-96
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• 1985

This study was conducted to establish the maternal haploid method for the practical breeding of M tabacum using the interspeciflc hybridization between M tabacum and N. aflicana. The frequency of surviving seedling per seed capsule of interspecific hybridization was 4.15. Among them, the frequencies of maternal haploid and hybrid were 1.20 and 2.95, respectively. The chromosome numbers of n=24 for maternal haploid and 2n=47 for hybrid were identified in surviving seedling from interspecific hybridiztion. Except the chromosome number, distinguishable morphological differences of material haploid from hybrid were observed at seedling stage.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• Journal of Plant Biology
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• v.34 no.1
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• pp.37-43
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• 1991

Mesophyll protoplasts of Nicoliana labacum ($NR^{-}/SR^{+}$) and N glulinosa were electrofused with AC field of 0.5 MHz and 1 kV DC pulse for 2 ms. Fused protoplasts were selected and cultured to the green cell clusters in $MSNO_3$ medium containing 1.2 mg!ml streptomycin sulfate. Four plant lines regenerated from selected colonies showed both parental morphological characteristics of leaf and flower and these plant lines were confirmed as somatic hybrids based on electrophoretic patterns of leaf peroxidase. In XhoI restriction patterns of chloroplast DNA, these hybrid plant lines expressed both parent common restriction sites and parent specific sites. One of these hybrid lines exhibited interspecific pattern of both parental chloroplast genomes. indicating nine both parent common sites, one N labacum specific site and two N glutinosa specific sites. sites.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.19-24
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• 2000

To obtain the genetic information about DVT( $\alpha$- and $\beta$-4,8,13- duvatriene-1,3-diols) and cis-abienol contents, two burley(Burley 21, KB 108), three sun-cured cultivars(N. tabacum L. cv. Yonbyun 3, Jahengyon and Jaraehong), TI 1068, and thirty F$_1$ hybrids derived from them were sampled and the diterpenes were analysed using thin layer chromatography (TLC) procedures. DVT exudation from the leaf scerface could Ie detected in all coltivars and F$_1$ hybrids tested. TI 1068 and Yonbyun 3 had cis-abienol exdudates. Burley 21, KB 108, Jaheungyon, and Jaraehong had no spot of cis-abienol. It is considered that cis-abienol exdudation might be controlled by dominant gene(s). The cytoplasmic effect on the cis-abienol exdudation was not detected.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.94-95
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• 1988

• Journal of Plant Biology
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• v.18 no.4
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• pp.150-154
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• 1975

Four kinds of Nicotiana species and five varieties belonging to N. tabacum were used as materials for electrophoretic analysis of the tetrazolium oxidase isozyme to examine the taxonomic affinity among them based on the biochemical property. All the five verieties of N. tabacum showed same isozyme bands despite the fact that these varieties had notably varied characteristics including morphological traits. The band patterns were more or less different among the four species. Although N. rustica and N. tabacum were of the same genome group of AABB, their isozyme bands showed considerable difference. N. sylvestris, genome A donor of Nicotiana species, was found to be markedly different from N. tatacum in band pattern, including the absence of system 2 in N. sylvestris.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.48-51
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• 1991

A program was initiated to transfer potato virus Y vein-necrosis strain resistance from N. africana to N. tabacum The Fl plants between the above species were self-sterile, but all amphidiploid plants from the Fl plants and backcrossed flowers, that is, the N. tabacum flowers crossed with amphidiploid were self-fertile. The parent, amphidiploid plants of Fl, F2 population of the amphidiploid and the backcrossed generation were screened for a resistance of potato virus Y vein-necrosis strain isolated in Korea. The Chi-square values for the F2 population of the amphidiploid and the backcrossed generation fitted 35: 1 and 5 : 1 ratios of resistant to susceptible for the potato virus Y vein-necrosis strain, respectively. Therefore, it was found that the resistance of N. tabacum for the potato virus Y vein-necrosis strain was controlled by a single dominant gene.