• Asian-Australasian Journal of Animal Sciences
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• v.30 no.8
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• pp.1143-1149
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• 2017

Objective: The objective of the present study was to determine whether mango saponin (MS) could be used as a feed additive in broiler chicks by evaluating growth performance, carcass characteristics, meat quality, and plasma biochemical indices. Methods: A total of 216 1-d-old Arbor Acres male broiler chicks were randomly assigned into three dietary treatments supplemented with 0 (control), 0.14% (MS 0.14%), or 0.28% (MS 0.28%) MS. Each treatment had six replicates (cages) with 12 chicks each. The feeding trial lasted for six weeks. Results: Compared with the control, dietary supplemented with 0.14% or 0.28% MS increased average daily weight gain of chicks in the grower (22 to 42 d) and the whole (1 to 42 d) phases, and the final body weight of chicks on d 42 was higher in MS supplemented groups (p<0.05). Lower $L_{45min}{^{\star}}$ (lightness) and $L_{24h}{^{\star}}$ values, lower $b_{24h}{^{\star}}$ (yellowness) value, and higher $a_{45min}{^{\star}}$ (redness) and $a_{24h}{^{\star}}$ values of the breast muscle were observed in chicks fed with 0.28% MS on d 42 (p<0.05). The total antioxidant capacity in plasma increased in MS 0.14% group on d 21 (p<0.001). Lower contents of plasma total cholesterol and triglyceride were observed in chicks fed with 0.28% MS on d 21 and d 42, whereas the group supplemented with 0.14% MS only decreased plasma triglyceride content on d 21 (p<0.05). The glucose content in plasma decreased in MS 0.28% group on d 42 (p<0.001). Conclusion: Overall, MS could be used as a feed additive in broiler chicks, and the supplemental level of 0.28% MS in diet could improve growth performance, meat quality, and plasma lipid metabolism in broiler chicks.

• Korean Journal of Soil Science and Fertilizer
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• v.5 no.1
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• pp.33-39
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• 1972

For the increase of productivity, canopy structure and nutritional status in various productivity grades (high>700, medium>600 and low>500kg/l0a as head rice) were investigated in a study using Jinheung(Oryza sativa L.) for the increase of producticity with following results: 1. High yield plot showed greater leaf area and slow decrease of it after heading stage. While low yield plot had rapidly decrease of leaf area. 2. High yield plot showed higher light transmission ratio (LTR) durnig early grow stage, low LTR. during late grow stage, and higher leaf area per LTR. 3. Net assimilation ratio was decrease with the increase of leaf area index (LAI) and it was always higher in high yield plot. 4. High yield plant had greater amount of total nitrogen uptake, slow uptake rate, and considerable uptake after heading while low yield plant showed higher uptake of nitrogen in early stage of growth and no uptake after heading. 5. The percent ratio starch at heading to total at harvesting was 40% for low yield plant and 10% for highyield plant inderting that high yield depends much on past-heading starch synthesis. 6. Main factor determining secondary yield was ripened grain ratio.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.4
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• pp.49-55
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• 1986

The authors carried out an experiment to determine the horizontal opening of the mid water trawl gear, which is the same as was used in the foreward experiment concerned to the attitude and opening efficiency of otter board. The trawl net .used in the experiment was designed to have a square sectional four-seamed body with two three-sepenlted wings. Each wing tip was rigged with a net pendant of 70 m long and the fore ends of the pendants were directly jointed to the otter pendant without inserting hand rope, and a front weight of 200 kg weigh was rigged with in front of the lowest wing tip. To determine the opening between otter boards ani between wing tips, two 50 KHz fish finders were set sideways on the otter board and on the port middle wing tip so as to detect the distance froa the finders to the starboard otter board and to the wing tip respectively, and the informations were transmitted to the indicators onboard the ship through water-tight cables. The results obtained can be summarized as follows: 1. The openings between otter borads were 43-45 m in case of the warp 100 m, 53-55 m in the war;> 150 m and 54-59 m in the warp 200 m. It was increased in linear according to the increase of towin; speed in the range of 1. 1-1. 8 mlscc. 2. The determined values of the openings between otter boards were greater than the estimated values. which were calculated by the opening between warps at the gallows of the ship and the length of tt~ warp, assuming that the warps from the top rollers to the otter boards were straight. The rates of the determined values to the estimated values were 1. 23-11. 0 in case of the warp 100 m, 1. 23-1. 12 in the warp 150 m and 1. 23-1. 15 in the warp 200 m respectively. The rates were almost the same at the slow towing speed of 1. 3 mlscc or so, then the higher the speed, the less the rate, and the longer the warp, the greater the rate. 3. The rates of the openings between otter boards to the total length of net pendant and the net (excluded cod end) showed 43-43% in case of the warp 150-200 m. It was a little smaller than th~ standard. which is 50%. in the common bottom trawl. 4. The determined openings between wing tips were 18-19 m in case of the warp 100m, 21-22 m in the warp 150 rn, They showed that the higher the speed, the greater the openings even though its range was no more than 1 m or so. 5. The rates of openings between otter boards to th~ openings betwee:l wing tips were almost invariably 38%. 6. The rates of openigs b2tween otter boards to the lenth of h~ad rope were 60-65% in cas': of th~ warp lOa-150m. It were much larg2r than th~ standard. which is 50%. in the common botto:n trawl.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.976-982
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• 2007

This study investigated whether supplemental Cu, Fe, Zn, and Mn are needed in a practical diet for pullets. Four hundred and twenty females of an egg-laying strain (1-d-old, Lohmann Brown Layer) were randomly distributed into 4 groups, consisting of 7 replicates of 15 birds each. During the 18-week experimental period, chicks were given three basal diets in sequence, each with single or multiple Mn, Zn and Cu supplementation to improve the mineral balance gradually. In the Control, no Mn, Zn, and Cu were added; in the single Mn supplemented group (sMn) Mn was added to 120, 60, and 60 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn and Zn supplemented group (mMnZn), Mn was added to 180, 90, and 90 mg/kg and Zn was added to 120, 105, and 105 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn, Zn, Cu supplemented group (mMnZnCu), Mn, Zn, and Cu were added to the same multiple of basal Fe concentration relative to NRC (1994) recommendations. Energy and protein metabolizability were determined by subtracting energy/protein intake by energy/protein excretion (from both feces and urine) and dividing by energy/protein intake. There were no statistically significant differences between groups in terms of feed intake, final body weight or tibia length throughout the experiment. Optimal growth performance was observed in the Control, while adding trace minerals to basal diets tended to result in decreased productive performance. Protein metabolizability was increased by mMnZn and mMnZnCu treatments, but energy metabolizability was not affected. Concentrations of Mn, Zn, Cu in excreta varied greatly related to dietary content, and the retentions of Cu, Fe, Zn and Mn were all increased due to the improvement of mineral balance. Based on these results, it is suggested that the concentrations of Cu, Fe, Zn and Mn in typical basal diets used in this study were adequate for normal growth for pullets from 1 to 18 weeks of age.

• Journal of Applied Biological Chemistry
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• v.54 no.1
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• pp.63-66
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• 2011

The bark of Machilus thunbergii was extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), butanol (n-BuOH), and $H_2O$, successively. From the EtOAc fraction, five lignans were isolated through the repeated silica gel, octadecyl silica gel (ODS) and, Sephadex LH-20 column chromatography. Based on nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared spectroscopy (IR) spectroscopic data, the chemical structures of the compounds were determined to be machilin A (1), machilin F (2), licarin A (3), nectandrin A (4), and nectandrin B, (5). This study presents comparative account of five lignans from M. thunbergii bark contributing inhibition of low density lipoprotein (LDL), ACAT-1, and ACAT-2. Compounds 2-5 showed varied degree of antioxidant activity on LDL with $IC_{50}$ values of 2.1, 11.8, 15.3, and $4.1{\mu}M$. Compounds 1, 2, and 3 showed inhibition activity on ACAT-1 with values $63.4{\pm}6.9%$ ($IC_{50}=66.8{\mu}M$), $53.7{\pm}0.9%$ ($IC_{50}=109.2{\mu}M$), and $78.7{\pm}0.2%$ ($IC_{50}=40.6{\mu}M$), respectively, at a concentration of 50 mg/mL, and on ACAT-2 with values $47.3{\pm}1.5%$ ($IC_{50}=149.7{\mu}M$), $39.2{\pm}0.2%$ ($IC_{50}=165.2{\mu}M$), and $52.1{\pm}1.0%$ ($IC_{50}=131.0{\mu}M$, respectively, at a concentration of 50 mg/mL.

• Korean Journal of Biological Psychiatry
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• v.24 no.1
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• pp.39-44
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• 2017

Objectives Angiotensin-converting enzyme (ACE) gene and plasma levels of cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), have previously been determined to be associated with depression. The purpose of this study was to investigate the association of plasma levels of ACE and cytokines with recurrent depression. Methods A total of 52 participants (14 male, 38 female, aged $43.9{\pm}14.0years$) were enrolled after being diagnosed with depression by experienced psychiatrists using the Mini International Neuropsychiatric Interview from the outpatient clinic of the Department of Psychiatry, CHA Bundang Medical Center. The participants completed blood sampling, the Hamilton Depression Rating Scale (HAMD), the Beck Depression Inventory, the Beck Anxiety Inventory, and the Scale for Suicidal Ideation. Results ACE plasma levels are higher in patients with recurrent depression ($27.4{\pm}10.4U/L$) than in patients with newly diagnosed depression ($19.1{\pm}7.7U/L$) (p = 0.004). The levels of cytokines, such as $TNF-{\alpha}$, IL-4, IL-6, IL-10, are not significantly different between the two groups. Additionally, the ACE plasma level is negatively correlated with a reduction in the HAMD over six weeks (r = -0.429, p = 0.046, n = 22). Conclusions The current findings show that plasma ACE levels may be associated with recurrent depression and further suggest that the renin-angiotensin system could play a role in recurrent depression.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.11
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• pp.1529-1539
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• 2012

A whole genome association (WGA) study was performed to detect significant polymorphisms for meat quality traits in an $F_2$ cross population (N = 478) that were generated with Korean native pig sires and Landrace dams in National Livestock Research Institute, Songwhan, Korea. The animals were genotyped using Illumina porcine 60k SNP beadchips, in which a set of 46,865 SNPs were available for the WGA analyses on ten carcass quality traits; live weight, crude protein, crude lipids, crude ash, water holding capacity, drip loss, shear force, CIE L, CIE a and CIE b. Phenotypes were regressed on additive and dominance effects for each SNP using a simple linear regression model, after adjusting for sex, sire and slaughter stage as fixed effects. With the significant SNPs for each trait (p<0.001), a stepwise regression procedure was applied to determine the best set of SNPs with the additive and/or dominance effects. A total of 106 SNPs, or quantitative trait loci (QTL) were detected, and about 32 to 66% of the total phenotypic variation was explained by the significant SNPs for each trait. The QTL were identified in most porcine chromosomes (SSCs), in which majority of the QTL were detected in SSCs 1, 2, 12, 13, 14 and 16. Several QTL clusters were identified on SSCs 12, 16 and 17, and a cluster of QTL influencing crude protein, crude lipid, drip loss, shear force, CIE a and CIE b were located between 20 and 29 Mb of SSC12. A pleiotropic QTL for drip loss, CIE L and CIE b was also detected on SSC16. These QTL need to be validated in commercial pig populations for genetic improvement in meat quality via marker-assisted selection.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.11
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• pp.1616-1624
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• 2016

The aim of present experiment was to investigate the effect of protein reduction in commercial broiler chicken rations with incorporation of de-oiled rice bran (DORB) and supplementation of limiting amino acids (valine, isoleucine, and/or tryptophan) with ration formulation either on total amino acid (TAA) or standardized ileal digestible amino acids (SIDAA). The experimental design consisted of $T_1$, TAA control; $T_2$ and $T_3$, 0.75% and 1.5% protein reduction by 3% and 6% DORB incorporation, respectively by replacing soybean meal with supplemental limiting amino acids to meet TAA requirement; $T_4$, SIDAA control, $T_5$ and $T_6$, 0.75% and 1.5% protein reduction by DORB incorporation (3% and 6%) with supplemental limiting amino acids on SIDAA basis. A total of 360 dold fast growing broiler chicks (Vencobb-400) were divided into 36 homogenous groups of ten chicks each, and six dietary treatments described were allocated randomly with six replications. During 42 days trial, the feed intake was significantly (p<0.05) reduced by TAA factor compared to SIDAA factor and protein factor significantly (p<0.05) reduced the feed intake at 1.5% reduction compared to normal protein group. This was observed only during pre-starter phase but not thereafter. The cumulative body weight gain (BWG) was significantly (p<0.05) reduced in TAA formulations with protein step-down of 1.5% ($T_3$, 1,993 g) compared to control ($T_1$, 2,067 g), while under SIDAA formulations, BWG was not affected with protein reduction of 1.5% ($T_6$, 2,076 g) compared to $T_4$ (2,129 g). The feed conversion ratio (FCR) was significantly (p<0.05) reduced in both TAA and SIDAA formulations with 1.5% protein step-down ($T_3$, 1.741; $T_6$, 1.704) compared to respective controls ($T_1$, 1.696; $T_4$, 1.663). The SIDAA formulation revealed significantly (p<0.05) higher BWG (2,095 g) and better FCR (1.684) compared to TAA formulation (2,028 g; 1.721). Intake of crude protein and all limiting amino acids (SID basis) was higher in SIDAA group than TAA group with resultant higher nitrogen retention (4.438 vs 4.027 g/bird/d). The nitrogen excretion was minimized with 1.5% protein reduction (1.608 g/bird) compared to normal protein group (1.794 g/bird). The serum uric acid concentration was significantly reduced in $T_3$ (9.45 mg/dL) as compared to $T_4$ (10.75 mg/dL). All carcass parameters were significantly (p<0.05) higher in SIDAA formulation over TAA formulation and 1.5% protein reduction significantly reduced carcass, breast and thigh yields. In conclusion, the dietary protein can be reduced by 0.75% with TAA formulation and 1.5% with SIDAA formulation through DORB incorporation and supplementation of limiting amino acids and among formulations, SIDAA formulation was better than TAA formulation.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.4
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• pp.268-274
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• 2006

Bacillus sp. DYL130 producing biosurfactant was isolated from soil samples in the Duck-yu mountain and identified as Bacillus sp. by analysis of 16S rDNA sequence. Purification of the biosurfactant was performed by using affinity chromatography and TLC. The biosurfactant of culture medium from Bacillus sp. DYL130 was eluted with 100% methanol using affinity chromatography. To remove methanol, a rotary evaporator was used and enrichment sample was dissolved in alkaline water(pH 10). The purified biosurfactant was identified by TLC. It was confirmed that the Rf value of the biosurfactant was 0.78. Antifungal activity against Botrytis cineria was showed the strongly activity as active antagonist. Maximum emulsification activity and stability were obtained from soybean oil. The critical micelle concentration (CMC) of purified biosurfactant was 35mg/l and the purified biosurfactant inhibited biofilm forming by Bacillus sp..

• Korean Journal of Plant Taxonomy
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• v.46 no.1
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• pp.65-82
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• 2016

Korean Peninsula Flora Database (KPF database), developed by T.B. Lee Herbarium of Seoul National University comprises ca. 65,000 accessions of vascular plants collected from Korean peninsula from 1850 to 1945. Among these, material from North Korea (Democratic People's Republic of Korea) is represented with ca. 33,000 accessions. The largest part of this material [ca. 4287 accessions (13%) from North Korea] originates from five Japanese and one American collectors, such as Imai, Hanjiro, Mills, Ralph Garfield, Furumi, Masatomi, Nomura, Naohiko, Saito, Tatsumoto, and Okuyama, Shunki from 1909 to 1942. These data are the third largest holding (13%) of North Korean collections after Komarov, V.L and Nakai, T. A part of scientific report about the results of these expeditions had been published before, but the present publication set it sights on giving a first overview of the itineraries and the materials collected by five collectors in North Korea. Among these, Saito has by far the largest collection with 1,730 specimens, followed by five collectors in order with 1,067, 532, 510, 368 and 370 accessions by Mills, Nomura, Okuyama, Furumi, and Imai respectively.