• Food Science and Preservation
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• v.12 no.3
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• pp.275-281
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• 2005

This study was carried out to investigate the cathepsin B inhibition effect by Achyranthes japonica N. root extract in vitro. The methanol/$H_{2}O$(4:1, v/v) extract was fractionated by ethyl acetate(F1), chloroform(F2), chloroform/methanol(3:1, v/v)(F3) and methanol(F4). The yield of F4 in Achyranthes japonica N. root was $8.27\%$. As an index material of Achyranthes japonica N. root, 20-hydroxy ecdysone was detected by TLC, and HPLC and it's content was $0.33\%$. Three isolates(F1, F3, F4) showed the cathepsin B inhibition activity, and F4 showed the highest inhibition activity among them. In the inhibition activity on cathepsin B, leupeptin, 20-hydroxy ecdysone and F4(at the same concentration of 20-hydroxy ecdysone.) were 92, 88 and $97\%$ on BANA($N{\alpha}$-benzoyl-DL-arginine ${\beta}$-naphthylamide) substrate, and 62, 36 and $67\%$ on CLN($N{\alpha}$-CBZ(carbobenzlyoxy)-L-lysine p-nitrophenyl ester HCI) substrate, respectively.

• Journal of Korean society of Dental Hygiene
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• v.21 no.4
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• pp.337-348
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• 2021

Objectives: To determine the usefulness of MiriChek^TM Oral Bacter as compared to traditional clinical methods in the periodontal assessment of dogs with and without periodontitis. Methods: In this study, 61 dogs were clinically examined using the MiriChek^TM Oral Bacter test kit, and information regarding breed, age (months), sex, weight, presence of disease, and periodontitis stage was recorded. The test used a sample of supragingival plaque from the upper left premolar of the dogs. Results: This study included 30 and 31 dogs with and without periodontitis, respectively. Periodontitis was more common in older dogs than in younger dogs. The average positive and negative sensitivity rates of the MiriChek^TM Oral Bacter test kit were 73.3% and 80.7%, respectively, and were both strongly correlated with the severity of periodontitis. Conclusions: The findings of this study encourage the use of new diagnostic assist test kits, such as MiriChek^TM Oral Bacter, for appropriately detecting and diagnosing periodontal disease in dogs.