• 한국산학기술학회논문지
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• 제21권11호
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• pp.728-739
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• 2020

이 연구는 웹보메트릭 빅데이터를 활용하여 코로나바이러스 확진 국가(지역)들의 온라인 정보원의 다양성을 조사했다. 구체적으로 2020년 2월에 코로나바이러스 확진자가 발생한 28개국을 대상으로 웹 데이터를 수집한 결과, 호주, 캐나다, 이탈리아 등의 온라인 가시성이 높게 나타나면서 관련 정보를 가장 많이 생산하고 있었다. 국가별 검색건수(hit counts)와 정보채널의 역할을 하는 도메인(domain) 빈도와는 통계적으로 유의한 상관성이 있었다. 한편 데이터 수집도구인 bing.com의 점유률이 평소에도 높은 국가들을 제외하고 다시 검토한 결과, 당시 확진자 수가 많았던 일본, 중국, 싱가포르 등이 코로나바이러스와 관련된 웹데이터를 주도적으로 올리고 있었다. 온라인 정보원은 n-헬릭스를 활용하여 분류되었다. n-헬릭스는 대학-기업-정부의 3주체간 상호작용과 혁신을 강조하는 트리플헬릭스모델(Triple Helix Model)에 기반한 확장된 분석틀이다. 그 결과, 정부기관이 18.1%를 차지하면서 코로나바이러스 정보의 최대 공급자로 나타났다. 2원성 네트워크 분석결과를 보면 언론사, 대학병원, 공중보건에 특화된 조직 등도 코로나바이러스 연구와 방역 정보의 온라인 유통에 적극적이었다. 웹페이지에 포함된 단어들을 중심으로 내용분석을 해 보니 건강, 학교, 가족, 공공, 방안 등의 단어가 중심성이 높게 나타나 코로나바이러스로 인한 개인별 예방수칙뿐만 아니라 생활 불편과 업무장애로 인한 대처방안 등에 관심이 높다는 것을 알 수 있었다.

• 공업화학
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• 제9권4호
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• pp.565-568
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• 1998

두 개 또는 세 개의 긴 알킬사슬을 갖고 두 개의 친수성기인 술폰산염기를 갖는 제미니형 양친매성 화합물들은 긴 사슬의 지방알코올을 가지고 상전이 촉매하에 합성된 N-acyldiethanolamine diglycidyl ether들과 반응에 의하여 합성한 것을 propanesulatone과 다시 반응하여 얻었다. 새로운 조성을 갖는 제미니형 계면활성제들은 모두 물에 용해성이 있으며 미셀형성이 낮은 농도에서 쉽게 이루어졌고, 하나의 술폰기와 소수성 알킬기를 갖는 기존의 계면활성제보다 낮은 표면장력 저하능을 보였고, cmc와 ${\Upsilon}$ cmc 값은 세 개의 사슬을 갖는 화합물이 두 개의 사슬을 갖는 화합물보다 적은 값을 나타내었다. 이들 화합물 모두가 물/공기 계면에서 흡착성이 매우 높았으며, 이들의 기포성, 습윤성 및 lime-soap dispersing requirement (LSDR)에 대한 물성을 측정하였다. 그 결과 초기 기포성은 좋지만 저기포안정성을 나타내었으며 습윤성 및 lime-soap dispersing repuirement (LSDR)도 양호한 특성을 나타내었다.

• BMB Reports
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• 제36권5호
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• pp.505-507
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• 2003

One of the small proteins from Helicobacter pylori, acyl carrier protein (ACP), was investigated by NMR. ACP is related to various cellular processes, especially with the biosynthesis of fatty acid. The basic NMR resonance assignment is a prerequisite for the validation of a heterologuous protein interaction with ACP in H.pylori. Here, the results of the backbone $^1H$, $^{15}N$, and $^{l3}C$ resonance assignments of the H. pylori ACP are reported using double- and triple-resonance techniques. About 97% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances that cover 76 of the 78 non-proline residues are clarified through sequential- and specific-assignments. In addition, four helical regions were clearly identified on the basis of the resonance assignments.

• BMB Reports
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• 제38권5호
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• pp.591-594
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• 2005

One of the small proteins from Helicobacter pylori, HP1242, was investigated by the solution nuclear magnetic resonance (NMR) spectroscopy. HP1242 is known as a 76-residue conserved hypothetical protein and its function cannot be identified based on sequence homology. Here, the results of the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignments of the HP1242 are reported using double- and triple-resonance techniques. About 95% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances that cover 75 non- Proline residues of the 76 residues are clarified through sequential- and specific- assignments. In addition, three helical regions were clearly identified on the basis of the resonance assignments.

• 한국동물위생학회지
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• 제17권1호
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• pp.25-31
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• 1994

In order to investigate the internal parasitic infection, fecal samples were collected from weaning pig(n=123), porker(n=418) and sow(n=121) in 49 sawdust fermentative pigsty of Chonbuk district. The prevalence and identification of internal parasites were determined by the fecal examination using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows ; 1. The detection rate of parasite - eggs from 662 fecal samples was 86.6%. 2. The infection rate of parasite-egg 96.4% in porker, 76.9% in sow, 62.6% in weaning pig, in order. 3. In the concern of mired infection such as single, double triple and quadraple, the rate was 42.3%, 28.7%, 12.2% and 3.3%, respectively. 4. Ten kinds of the detected eggs were isolated from 662 fecal samples. They were classified as Balantidium coli (63.6%), Trichuris suis(24.8%), isospora spp.(23.5%), Oesoohangostomum spp.(17.8%), Ascaris suum(11.8%), Hyostronylus rubiddus (2.8%), strongyloides spp. (1.7%), Gnathostoma spp. (1.5%), Stephanurus dentatus(1.3%) and Metastrongylus spp. (0.7%), in order.

• 한국전기전자재료학회논문지
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• 제22권9호
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• pp.781-785
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• 2009

We have fabricated simple triple-layer blue-emitting phosphorescent organic light emitting diodes (OLEDs) using different thicknesses (25 and 55 nm) of 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline (BCP) electron transport layers. 1,1-bis[4-bis (4-methylphenyl)- aminophenyllcyclohexane (TAPC), bis[(4,6-di-fluorophenyl)-pyridinate-$N,C^{2'}$]picolinate (FIrpic) and N,N' -dicarbazolyl-3,5-benzene (mCP) were used as hole transport, blue guest and host materials, respectively. The driving voltage, electroluminescence (EL) efficiency and emission characteristics of devices were investigated. The maximum EL efficiency was 20 cd/A in the device with 55 nm BCP layer, which efficiency was about 33% higher than the device with 25 nm BCP layer. The higher efficiency in the 55 nm BCP device resulted from the enhanced electron-hole balance. In the EL spectrum of blue phosphorescent OLED with BCP layer, the relative intensity between 470 and 500 nm peaks was related to the location of emission zone.

• 대한의생명과학회지
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• 제27권3호
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• pp.134-141
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• 2021

c-Jun N-terminal kinases (JNKs) have a Janus face, regulating both cell apoptosis and survival. The present study focused on understanding the function of JNK in tumor development and the chemoresistance underlying JNK-mediated cancer cell survival. We identified an inhibitor of JNK1, an important regulator of cancer cell survival. Kinase assay data showed that JNK1-dependent c-Jun phosphorylation was inhibited by indirubin derivatives. In particular, indirubin-3-monoxime (I3M) directly inhibited the phosphorylation of c-Jun in vitro, with a half inhibition dose (IC₅₀) of 10 nM. I3M had a significant inhibitory effect on JNK1 activity. Furthermore, we carried out assays to determine the viability, migration, and proliferation of breast cancer cells. Our results demonstrated that cell growth, scratched wound healing, and colony forming abilities were inhibited by the JNK inhibitor SP600125 and I3M. The combination of SP600125 and I3M significantly decreased cancer cell proliferation, compared with either SP600125 or I3M alone. Our studies may provide further support for JNK1-targeting cancer therapy using the indirubin derivative I3M in breast cancer.

• Mass Spectrometry Letters
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• 제13권2호
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• pp.35-40
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• 2022

Human tissues and organs can be preserved intact by fixation with formalin for the future analysis of biomolecules of interest. With the advances in high-throughput methods, numerous protocols have been developed and optimized to attain the most pathophysiological information out of biomolecules, including RNA and proteins, in formalin-fixed samples. However, there is no systematic study to examine the effects of formalin fixation on the lipidome of biological samples in a global fashion. In this study, we conducted a mass spectrometry-based analysis to survey the alteration in the lipidome of mice brains by fixation methods. A total of 308 lipids were quantitatively measured using triple quadrupole mass spectrometry. We found that most were unchanged after formalin fixation except for a few lipid classes such as phosphatidylethanolamine.

• 한국소프트웨어감정평가학회 논문지
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• 제16권2호
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• pp.143-151
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• 2020

인터넷을 기반으로 하는 디지털 컨텐츠 응용 분야에서 디지털 콘텐츠의 보안 및 인증은 매우 중요하다. 특히, 콘텐츠의 인증을 수행하는 방법에는 여러 가지가 있다. 디지털 워터 마킹은 이와같은 방법 중 하나이다. 특히, 논문은 디지털 이미지의 응용에서 동작하는 디지털 워터마크 기반 인증 방법을 제시한다. 제안 된 워터마크는 3 상태 정보를 가지고 있으며 참조 콘텐츠 없이 임베딩 및 검출을 수행하는 블라인드 워터마킹 방법이다. 디지털 콘텐츠의 소유자 정보를 인증 할 때 자기 상관 함수를 사용한다. 또한, 주파수 영역(DWT Domain)에서 원본 콘텐츠의 신호에 적응적이도록 대역 확산(Spread Spectrum) 방식을 사용한다. 따라서 은닉정보의 검출에서 일어나는 오류의 가능성을 줄였다. DWT에서의 워터마킹은 다른 변환 방법 (DFT, DCT 등)보다 빠른 은닉/검출의 장점을 가진다. 크기가 N=m X m인 이미지를 사용할 때의 계산량은 2상태 워터마크는 O (N·logN)인 반면 O (N)으로 줄어들 수 있다. 가장 우수한 장점은 비트 당 더 많은 정보를 숨길 수 있다는 것이다.

• 한국동물위생학회지
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• 제45권1호
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• pp.31-38
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• 2022

Swine influenza (SI) is an important respiratory disease in pigs and epidemic worldwide, which is caused by influenza A virus (IAV) belonging to the family of Orthomyxoviridae. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs, and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/21810/2021 (sw21810, H3N2 subtype). BLASTN sequence analysis of 8 gene segments of the isolated virus revealed a high degree of nucleotide similarity (94.76 to 100%) to porcine strains circulating in Korea and the United States. Out of 8 genome segments, the HA gene was closely related to that of isolates from cluster I. Additionally, the NA gene of the isolate belonged to a Korean Swine H1N1 origin, and the PB2, PB1, NP and NS genes of the isolate were grouped into that of the Triple reassortant swine H3N2 origin virus. The PA and M genes of the isolate belonged to 2009 Pandemic H1N1 lineage. Human infection with mutants was most common through contact with infected pigs. Our results suggest the need for periodic close monitoring of this novel swine H3N2 influenza virus from a public health perspective.