• Title/Summary/Keyword: N-R Method

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Characterization and screening of pyrrolizidine alkaloids by UPLC-MS/MS: Application to honey (UPLC-MS/MS를 이용한 벌꿀제품의 피롤리지딘 알칼로이드 잔류실태 및 분석법 선진화)

  • Ryu, Hoejin;Kim, Oukhee;Lee, Eunsoon;Kim, Misun;Kim, Jeong-gon;Yun, Eunsun;Kim, Hyunjeong;Kim, Musang
    • Analytical Science and Technology
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    • v.32 no.6
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    • pp.252-261
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    • 2019
  • Pyrrolizidine alkaloids (PAs) are a group of secondary metabolites that are produced by plants all over the world as a defense mechanism against herbivores. To date, over 660 PAs have been identified from more than 6,000 plant species that have been reported to be widely present in plants belonging to Asteraceae, Boraginaceae, and Fabaceae. This study describes an analytical method based on UPLC-MS/MS for the quantitation of 7 pyrrolizidine alkaloids (Lycopsamine, Echimidine, Retrorsine, Retrorsine N-oxide, Senecionine, Heliotrine, and Trichodesmine) in honey, and was applied to 84 honey samples for validation. Quantitation was performed based on a matrix-matched calibration to compensate for the matrix effect on the electrospray ionization. Good linear calibrations were obtained for all 7 PAs in the spiked honey samples (2.575-202.14 ㎍/kg; R2 ≥ 0.998). The extraction recoveries for most of the PAs in the honey samples were in the range of 81 %-108 %. The analysis showed that 6 of the 84 honey samples were contaminated by the PAs with the mean total sum of PAs being 47.19 ㎍/kg, and the concentrations of the PAs were observed to be in the range of 1.76-202.1 ㎍/kg. The retronecine type compounds (Echimidine, Lycopsamine) were the most frequently found PAs in honey. These data provide useful information for the assessment of human risk posed by the consumption of honey contaminated PAs.

DETERMINATION OF MOISTURE AND NITROGEN ON UNDRIED FORAGES BY NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS)

  • Cozzolino, D.;Labandera, M.;Inia La Estanzuela
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1620-1620
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    • 2001
  • Forages, both grazed and conserved, provide the basis of ruminant production systems throughout the world. More than 90 per cent of the feed energy consumed by herbivorous animals world - wide were provided by forages. With such world - wide dependence on forages, the economic and nutritional necessity of been able to characterize them in a meaningful way is vital. The characterization of forages for productive animals is becoming important for several reasons. Relative to conventional laboratory procedures, Near Infrared Reflectance Spectroscopy (NIRS) offers advantages of simplicity, speed, reduced chemical waste, and more cost-effective prediction of product functionality. NIR spectroscopy represents a radical departure from conventional analytical methods, in that entire sample of forage is characterized in terms of its absorption properties in the near infrared region, rather than separate subsamples being treated with various chemicals to isolate specific components. This forces the analyst to abandon his/her traditional narrow focus on the sample (one analyte at a time) and to take a broader view of the relationship between components within the sample and between the sample and the population from which it comes. forage is usually analysed by NIRS in dry and ground presentation. Initial success of NIRS analysis of coarse forages suggest a need to better understand the potential for analysis of minimally processed samples. Preparation costs and possible compositional alterations could be reduced by samples presented to the instrument in undried and unground conditions. NIRS has gained widespread acceptance for the analysis of forage quality constituents on dry material, however little attention has been given to the use of NIRS for chemical determinations on undried and unground forages. Relatively few works reported the use of NIRS to determine quality parameters on undried materials, most of them on both grass and corn silage. Only two works have been found on the determination of quality parameters on fresh forages. The objectives of this paper were (1) to evaluate the use of NIRS for determination of nitrogen and moisture on undried and unground forage samples and (2) to explore two mathematical treatments and two NIR regions to predict chemical parameters on fresh forage. Four hundred forage samples (n: 400) were analysed in a NIRS 6500 instrument (NIR Systems, PA, USA) in reflectance mode. Two mathematical treatments were applied: 1,4,4,1 and 2,5,5,2. Predictive equations were developed using modified partial least squares (MPLS) with internal cross - validation. Coefficient of determination in calibration (${R^2}_{CAL}$) and standard error in cross-validation (SECV) for moisture were 0.92 (12.4) and 0.92 (12.4) for 1,4,4,1 and 2,5,5,2 respectively, on g $kg^{-1}$ dry weight. For crude protein NIRS calibration statistics yield a (${R^2}_{CAL}$) and (SECV) of 0.85 (19.8) and 0.85 (19.6) for 1,4,4,1 and 2,5,5,2 respectively, on a dry weight. It was concluded that NIRS is a suitable method to predict moisture and nitrogen on fresh forage without samples preparation.

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Preliminary study on the use of near infrared spectroscopy for determination of plasma deuterium oxide in dairy cattle

  • Purnomoadi, Agung;Nonaka, Itoko;Higuchi, Kouji;Enishi, Osamu;Amari, Masahiro;Terada, Fuminori
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.4101-4101
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    • 2001
  • Information of body composition (fat and protein) in living animal is important to determine the nutrients requirement. Deuterium oxide (D2O) dilution techniques, as one of isotope dilution techniques have been useful for the prediction of body composition. However, the determination of D2O concentration is time consuming and complicated. Therefore this study was conducted to develop a new method to predict D2O concentration in plasma using near infrared spectroscopy technique (NIRS). Four dairy cows in early lactation were used. They were fed total mixed ration containing conr silage, timothy hay, and concentrates to make 17.0%CP and 14.0 MJDE/kgDM. Dosing D2O was at week 1,3 and 5 after parturition. After dosing D2O, the blood was collected from hour 0 to 72. Blood samples were then centrifuge at 3,000 rpm for 10 minutes to obtain plasma. D2O concentration was analyzed by gas chromatograph (deuterium oxide analyzable system, HK102, Shokotsusyou) after extracted from plasma by liophilization. Plasma sample was scanned by NIRS using Pacific Scientific (Neotec) model 6500 (Perstorp Analytical, Silver Spring, MD) in the range of wavelength from 1100 to 2500 nm. Calibration equation was developed using multiple linear regression. Sample from one animal (cow #550; n: 74) was used for developing the calibration while the rest three animals were used for validating the equation. The range, R and SEC of the calibration set samples were 135-925 ppm, 0.93 and 48.1 ppm, respectively. Validation of the calibration equation for three individual cows was done and the average of NIR predicted value of D2O at each collection time from three weeks injection showed a high correlation. The range, r and 53 of plasma from cow #474 were 322-840 ppm,0.93 and 53.1; cow #478 were 146-951 ppm,0.95 and 39.8; cow #942 were 313-885 ppm,0.95 and 37.2, respectively. Judgement of accuracy based on ratio of standard deviation and standard error in validation set samples (RPD) for cow #474, #478 and #942 were 2.2,4.3 and 3.4, respectively. The error in application due to the variation between individual was considered smaller than the bias from collection period, however, this prediction can be overcome with correction of standard zero-minute concentration of blood. The results of this preliminary study on the use of NIRS for determination of D2O in plasma showed very promising as shown by a convenient and satisfy accuracy. Further study on various physiological stage of animal should be done.

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Analysis of Typhoon for Design of Sea-Dike (방조제의 설계를 위한 태풍의 분석)

  • 한상욱;이중기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.18 no.2
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    • pp.4089-4095
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    • 1976
  • The safety of shore structure including the sea dipe is largely affected by typhoon. Accordingly it is desirable to analize the typhoon and determine the wind direction and velocity for use in planning and design of the structure. This method was adopted for the design of the Yong San Gang Estuary Dam. A comparative study of the results of typhoon analysis with the meteorological data obtained through actual observation is summarized as follows; (1) 62% of the typhoons occur during May to June in a year, and 62% of the typhoons which have an influence on the Korean peninsula, especially the proposed estuary dam fsite, proceed eastward through the zone in lat. 36$^{\circ}$-37$^{\circ}$N. Such typhoons occur two to three times a year on the average. (2) Data on typhoon "SARL" were used as a model case in designing the estuary dam, where it was proved that a southwesterly wind had a maximum velocity of 30m/sec in case r=150km, ${\alpha}$=120$^{\circ}$. Within the range of 22$^{\circ}$30'on the right and left side of the fetch line of the estuary dam, the wind direction varied SSW\longrightarrowSW\longrightarrowWSW, and the wind velocity varied 29m/sec\longrightarrow30m/sec\longrightarrow125m/sec. Such phenomemum lasted for five hours. (3) An analysis of data obtained during 44 years at Mok Po Meteorological Station shows that a wind with a velocity of some 25m/sec occurred twelve times in the S-direction and two times in the SW-direction, while that with a velocity of 30m/sec occurred three times in the S-direction, three times in the SSW-direction and one time in the SW-direction. The wind which had an influence on the estuary dam had a direction of SSW\longrightarrowSW\longrightarrowWSW and a velocity of min. 30m/sec. Actually, a wind with a max. velocity of 31.3m/sec occurred in the SSW-direction on March 15 and 16, 1956 where the mean velocity during two hours was 28m/sec and that during four hours was 24.6m/sec. (4) The data obtained through actual observation show that when the velocity is low, the wind with a fixed direction lasts long, and when the velocity is high, it is short-lived. It is difficult to determine the velocity of a wind which blows in a fixed direction for consecutive two or four hours. Therefore, the values obtained through typhoon analysis are larger that those obtained through actual observation, and hence, it is resonable to use the analyzed valuse for design of the estuary dam and shore structures. (5) The greatest effect was had on the estuary dam when typhoon was proceeding at a velocity of 29.71m/sec in the direction of ${\alpha}$=120$^{\circ}$(SW) at a point of R=150km from the center of the typhoon.

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The Analysis on the Academic Information Seeking Behavior of College Professors: A Social Scientist Group in the K-University (대학교수들의 학술정보원 이용행태분석: K대학의 사회과학교수집단을 중심으로)

  • 박준식;박일종;신상헌
    • Journal of the Korean Society for information Management
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    • v.17 no.3
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    • pp.21-43
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    • 2000
  • Not only the infomatmn seeking bellavmr or acadenuc sources by social scientists was analyzed bnt its eiieclive usmg method was also presented in this paper. For thc purpose, the questionnaires were prepared md admmislered lo social sacnilsin who are the profcsso~s oi a large unlverslty m Taegu, Korea. and the survey for tiler academic environment and its sateiaclion, characterislics, inforn~ahon seeking behavior of academic sources, etc. was conducted. Also, iheir research mlercsts and period, informauon s e e h g behav~or and its dfficulties, reineval melhods, and uslng rrequcncies of current issues elc. were analyzed in lliis paper. The major hdings ol the quanm.live pa on the development 01 academic sources for soaal sciencc study and t11e11 uses for education md study m !hi7 study are: (1) Socid sclenhsts preferred acquiring matenal ihrougli internet. CD-ROM, and PC, etc, lo the traditional methods; (2) They spend approxinmtciy 41 days for collecihg matend they wanl. and each of them has petty various period la collect n~atenal and acquiring methods. (standard deviation : about 35 days); (3) The cilalion kequencies of fore~gu material was hi&r than domeslic one; (4) They, social sc~entists, have a pretty hard time to access and use the theses, journal, magazinc. mmol~ches and m s r o f i , CD-ROM malerid etc. technicdly; (5) The group that has morc sat~sIaclion to thesa aud more fiequenl participant ol seminar seek [or more recen issues of a journal lhrough ihc research modcl of melag. (6) The group thal has higher positioned and 1on.a tenure o: office can access lo shorter time at period lhau the one that bas lhe lowcr and the shortcr group. Also, tutule research topic and recommcndations [or a ~ocial saentlst are proposed and suggested when using a social science material

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Are Biomarkers Predictive of Anthracycline-Induced Cardiac Dysfunction?

  • Malik, Abhidha;Jeyaraj, Pamela Alice;Calton, Rajneesh;Uppal, Bharti;Negi, Preety;Shankar, Abhishek;Patil, Jaineet;Mahajan, Manmohan Kishan
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.4
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    • pp.2301-2305
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    • 2016
  • Background: The early detection of anthracycline- induced cardiotoxicity is very important since it might be useful in prevention of cardiac decompensation. This study was designed with the intent of assessing the usefulness of cardiac troponin T (cTnT) and NT- Pro BNP estimation in early prediction of anthracycline induced cardiotoxicity. Materials and Methods: In this prospective study histologically proven breast cancer patients who were scheduled to receive anthracycline containing combination chemotherapy as a part of multimodality treatment were enrolled. Baseline cardiac evaluation was performed by echocardiography (ECHO) and biomarkers like cardiac troponin T (cTnT) and N terminal- pro brain natriuretic peptide (NT- Pro BNP). All patients underwent cTnT and NT- Pro BNP estimation within 24 hours of each cycle of chemotherapy and were followed up after 6 months of initiation of chemotherapy. Any changes in follow up ECHO were compared to ECHO at baseline and cTnT and NT- Pro BNP levels after each cycle of anthracycline-based chemotherapy. Results: Initial data were obtained for 33 patients. Mean change in left ventricular diastolic diameter (LVDD) within 6 months was $0.154{\pm}0.433cms$ (p value=0.049). Seven out of 33 patients had an increase in biomarker cTnT levels (p value=0.5). A significant change in baseline and follow up LVDD was observed in patients with raised cTnT levels (p value=0.026) whereas no change was seen in ejection fraction (EF) and left atrial diameters (LAD) within 6 months of chemotherapy. NT- Pro BNP levels increased in significant number of patients (p value ${\leq}0.0001$) but no statistically significant change was observed in the ECHO parameters within 6 months. Conclusions: Functional monitoring is a poorly effective method in early estimation of anthracycline induced cardiac dysfunction. Estimation of biomarkers after chemotherapy may allow stratification of patients in various risk groups, thereby opening window for interventional strategies in order to prevent permanent damage to the myocardium.

Anti-apoptotic and neuroprotective effects of acupuncture techniques of tonification or sedation at HT9${\cdot}$LR1, HT3${\cdot}$KI10 on focal brain ischemic injury induced by intraluminal filament insertion in rats (심정격(心正格) 혈위(穴位)(소충(少衝)${\cdot}$대돈(大敦), 소해(少海)${\cdot}$음곡(陰谷))에 대한 영수(迎隨) 및 염전(捻轉) 보사(補瀉) 침자(鍼刺)가 국소 뇌허혈 유발 흰쥐의 항뇌세포고사와 신경보호 효과에 미치는 영향)

  • Byun, Jeong-Yun;Youn, Dae-Hwan;Na, Chang-Su
    • Korean Journal of Acupuncture
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    • v.25 no.1
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    • pp.229-246
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    • 2008
  • Objectives : Acupoints of LR1(Dadun)${\cdot}$HT9(Shaochong), KI10(Yingu)${\cdot}$HT3(Shaohai) are used as controlling the diseases from heart problems. Especially, when they are used, tonification or sedation techniques method is taken as a controlling the medication for an early stroke in the Korean medicine. So the aim of this study is to investigate the anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR1${\cdot}$HT9, KI10${\cdot}$HT3 on the focal ischemia induced by intraluminal filament insertion in rats. Materials and Methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The animals were divided into five groups (n=7 in each group) : Control group, no therapy group after ischemia induced; AT-1; acupuncture therapy group at LR1${\cdot}$HT9, KI10${\cdot}$HT3 after ischemia-induced. AT-2; acupuncture therapy at right LR1, HT9(forward direction), KI10${\cdot}$HT3(opposite direction) inserting to the direction of route of the each meridian. AT3; acupuncture therapy at right LR1, HT9(twirling forward with the thumb of right hand 9 times), KI10${\cdot}$HT3(twirling forward with the forefinger of right hand 6 times) AT-4; acupuncture therapy at right LR1, HT9(forward direction, twirling forward with the thumb of right hand 9 times), KI10${\cdot}$HT3(opposite direction, twirling forward with the forefinger of right hand 6 times) inserting to the direction of route of the each meridian. The anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR1, HT9, KI10${\cdot}$HT3 were observed by Bax, Bcl-2, mGluR5, Cytochrome c, Cresyl violet and ChAT-stain. Results : The intensity of Bax and Bax/Bcl-2 ratio were increased in ACU-2 and ACU-4 group but decreased in ACU-3 group. The intensity of mGluR5 was increased in ACU-1, ACU-2, ACU-3 and ACU-4 group. The intensity of cytochrome c was decreased in ACU-3 and ACU-4 group. The density of neurons stained by Cresyl violet and ChAT were increased in ACU-1, ACU-3 and ACU-4 group. Conclusions : Our study suggests that acupuncture therapy of tonification at LR1, HT9 by twirling forward with the thumb of right hand 9times, sedation at KI10${\cdot}$HT3 by twirling forward with the forefinger of right hand 6 times after perpendicularly inserting needle shows anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

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A Study on the Molecular Weight Control and Rheological Properties of Branched Polycarbonate (분지형 폴리카보네이트의 분자량 조절 및 유변학적 특성 연구)

  • Lee, Bom Yi;Dahal, Prashanta;Kim, Hee Seung;Yoo, Seung Yoon;Kim, Youn Cheol
    • Applied Chemistry for Engineering
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    • v.23 no.4
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    • pp.388-393
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    • 2012
  • Branched polycarbonates (B-PCs) were synthesized using melt polymerization method with four different end capping agents and vaying concentrations. The chemical structure of the synthesized PC was determined by FT-IR and $^{1}H-NMR$ spectroscopy, and the reaction of the end capping agent was confirmed by the existence of hydroxy group in FT-IR spectrum. The average molecular weight and distribution, glass transition and degradation temperatures were determined by GPC, DSC and TGA. The average molecular weight changed with the chemical structure of end capping agent, and 4-tert-butylphenol was estimated as the optimum end capping agent. The average molecular weights of B-PCs decreased with the increase of the concentration of the agent, the number average molecular weight represented 20000 when 0.05 mol% of 4-tert-butylphenol was added to B-PCs. The melt viscosities of the B-PCs decreased with the decrease of the molecular weight of B-PCs, and adding of the agent was not effected to shear thinning tendency.

Impact Analysis of Overestimation Sources on the Accuracy of the Worst Case Timing Analysis for RISC Processors (RISC 프로세서를 대상으로 한 최악 실행시간 분석의 정확도에 대한 과예측 원인별 영향 분석)

  • Kim, Seong-Gwan;Min, Sang-Ryeol;Ha, Ran;Kim, Jong-Sang
    • Journal of KIISE:Computing Practices and Letters
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    • v.5 no.4
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    • pp.467-478
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    • 1999
  • 실시간 태스크의 최악 실행시간을 예측할 때 과예측이 발생하는 원인은, 첫째 프로그램의 동적인 최악 실행 행태를 정적으로 분석하는 것이 근본적으로 어렵기 때문이며, 둘째 최근의 RISC 형태 프로세서에 포함되어 있는 파이프라인 실행 구조와 캐쉬 등이 그러한 정적 분석을 더욱 어렵게 만들기 때문이다. 그런데 기존의 연구에서는 각각의 과예측 원인을 해결하기 위한 방법에 대해서만 언급하고 있을 뿐 분석의 정확도에서 각 원인이 차지하는 비중에 대해서는 언급하고 있지 않다. 이에 본 연구에서는 최악 실행시간 예측시 과예측을 유발하는 원인들, 즉 분석 요소들의 영향을 정량적으로 조사함으로써 기존의 최악 실행시간 분석 기법들이 보완해야 할 방향을 제시하고자 한다. 본 연구에서는 실험이 특정 분석 기법에 의존하지 않도록 하기 위하여 시뮬레이션 방법에 기반한다. 이를 위해 분석 요소별 스위치가 포함된 MIPS R3000 프로세서를 위한 시뮬레이터를 구현하였는데, 각 스위치는 해당 분석 요소에 대한 분석의 정확도 수준을 결정한다. 모든 스위치 조합에 대해서 시뮬레이션을 반복 수행한 다음 분산 분석을 수행하여 어떤 분석 요소가 가장 큰 영향을 끼치는지 고찰한다.Abstract Existing analysis techniques for estimating the worst case execution time (WCET) of real-time tasks still suffer from significant overestimation due to two types of overestimation sources. First, it is unavoidably difficult to predict dynamic behavior of programs statically. Second, pipelined execution and caching found in recent RISC-style processors even more complicate such a prediction. Although these overestimation sources have been attacked in many existing analysis techniques, we cannot find in the literature any description about questions like which one is most important. Thus, in this paper, we quantitatively analyze the impacts of overestimation sources on the accuracy of the worst case timing analysis. Using the results, we can identify dominant overestimation sources that should be analyzed more accurately to get tighter WCET estimations. To make our method independent of any existing analysis techniques, we use simulation based methodology. We have implemented a MIPS R3000 simulator equipped with several switches, each of which determines the accuracy level of the timing analysis for the corresponding overestimation source. After repeating simulation for all of the switch combinations, we perform the variance analysis and study which factor has the largest impact on the accuracy of the predicted WCETs.

Development of Near-isogenic Japonica Rice Lines with Enhanced Resistance to Magnaporthe grisea

  • Kwon, Soon-Wook;Cho, Young-Chan;Kim, Yeon-Gyu;Suh, Jung-Pil;Jeung, Ji-Ung;Roh, Jae-Hwan;Lee, Sang-Kyu;Jeon, Jong-Seong;Yang, Sae-Jun;Lee, Young-Tae
    • Molecules and Cells
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    • v.25 no.3
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    • pp.407-416
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    • 2008
  • Thirteen near-isogenic lines (NILs) of japonica rice were developed via a backcross method using the recurrent parent Chucheong, which is of good eating quality but is susceptible to Magnaporthe grisea, and three blast resistant japonica donors, Seolak, Daeseong and Bongkwang. The agro-morphological traits of these NILs, such as heading date, culm length, and panicle length, were similar to those of Chucheong. In a genome-wide scan using 158 SSR markers, chromosome segments of Chucheong were identified in most polymorphic regions of the 13 NIL plants, and only a few chromosome segments were found to have been substituted by donor alleles. The genetic similarities of the 13 NILs to the recurrent parent Chucheong averaged 0.961, with a range of 0.932-0.984. Analysis of 13 major blast resistance (R) genes in these lines using specific DNA markers showed that each NIL appeared to contain some combination of the four R genes, Pib, Pii, Pik-m and Pita-2, with the first three genes being present in each line. Screening of nine M. grisea isolates revealed that one NIL M7 was resistant to all nine isolates; the remaining NILs were each resistant to between three and seven isolates, except for NIL M106, which was resistant to only two isolates. In a blast nursery experiment, all the NILs proved to be more resistant than Chucheong. These newly developed NILs have potential as commercial rice varieties because of their increased resistance to M. grisea combined with the desirable agronomic traits of Chucheong. They also provide material for studying the genetic basis of blast resistance.