• Title/Summary/Keyword: N-R Method

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'Kresek' Disease in Korea III. Varietal Resistance, and Relationship among Pathotype, Multiplication in the Tissue and Disease Development (한국에 있어서의 'Kresek'에 관한 연구 III. 품종저항성 및 균주에 따른 도체내에서의 균증식과 발병과의 관계)

  • Choi Y. C.;Cho Y. S.
    • Korean journal of applied entomology
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    • v.19 no.1 s.42
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    • pp.51-55
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    • 1980
  • The study has been carried to confirm pathogenesity among the isolates which obtained from leaf blight type symptoms and Kresek type symptoms, and also to observe varietal resistance among 29 varieties and/or lines through the introduction of two different pathotypes, of Xanthomonas oryzae, by the use of root clipping and pin prick methods. There was no significant differences among the isolates when their growth in plant tissue were compared. There was certain tendency, however, that the isolates from Kreseked plants showed longer lesion than those from blight type lesions. Both isolates from blight type and Kresek type induced the same degree of Kresek syptoms when they were introduced into plants by root cutting in the suspension prior to transplant. Varietal resistance to 'Kresek' appeared to be the same with those on leaf blight type though Wase Aikoku group varieties showed Kresek symptoms which is not the case with leaf blight type. IR 20 in Kogyoku group varieties showed highly resistant reaction to Kresek type. Root clipping method induced the more of Kresek than those by pin pricking method.

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Simultaneous Determination of Cannabidiol, Cannabinol, and ${\Delta}^9-Tetrahydrocannabinol$ in Human Hair by Gas Chromatography-Mass Spectrometry

  • Kim Jin Young;Suh Sung Ill;In Moon Kyo;Paeng Ki-Jung;Chung Bong Chul
    • Archives of Pharmacal Research
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    • v.28 no.9
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    • pp.1086-1091
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    • 2005
  • An analytical method was developed for evaluating the cannabidiol (CBO), cannabinol (CBN), ${\Delta}^9-tetrahydrocannabinol$ $({\Delta}^9-THC)$ level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50mg) were washed with isopropyl alcohol and cut into small fragments (< 1mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0M NaOH for 10 min at $95^{\circ}C$. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and ${\Delta}^9-THC$ at three concentration levels were $37.9-94.5\%$ with good correlation coefficients $(r^2>0.9989)$. The intra-day precision and accuracy ranged from $-9.4\%\;to\;17.7\%$, and the inter-day precision and accuracy ranged from $-15.5\%\;to\;14.5\%$, respectively. The limits of detection (LOD) for CBD, CBN, and ${\Delta}^9-THC$ were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.

A Machine Learning Model for Predicting Silica Concentrations through Time Series Analysis of Mining Data (광업 데이터의 시계열 분석을 통해 실리카 농도를 예측하기 위한 머신러닝 모델)

  • Lee, Seung Hoon;Yoon, Yeon Ah;Jung, Jin Hyeong;Sim, Hyun su;Chang, Tai-Woo;Kim, Yong Soo
    • Journal of Korean Society for Quality Management
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    • v.48 no.3
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    • pp.511-520
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    • 2020
  • Purpose: The purpose of this study was to devise an accurate machine learning model for predicting silica concentrations following the addition of impurities, through time series analysis of mining data. Methods: The mining data were preprocessed and subjected to time series analysis using the machine learning model. Through correlation analysis, valid variables were selected and meaningless variables were excluded. To reflect changes over time, dependent variables at baseline were treated as independent variables at later time points. The relationship between independent variables and the dependent variable after n point was subjected to Pearson correlation analysis. Results: The correlation (R2) was strongest after 3 hours, which was adopted as a dependent variable. According to root mean square error (RMSE) data, the proposed method was superior to the other machine learning methods. The XGboost algorithm showed the best predictive performance. Conclusion: This study is important given the current lack of machine learning studies pertaining to the domestic mining industry. In addition, using time series analysis in mining data will show further improvement. Before establishing a predictive model for the proposed method, predictions should be made using data with time series characteristics. After doing this work, it should also improve prediction accuracy in other domains.

Low velocity impact response and dynamic stresses of thick high order laminated composite truncated sandwich conical shell based on a new TDOF spring-mass-damper model considering structural damping

  • Azizi, A.;Khalili, S.M.R.;Fard, K. Malekzadeh
    • Steel and Composite Structures
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    • v.26 no.6
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    • pp.771-791
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    • 2018
  • This paper deals with the low velocity impact response and dynamic stresses of composite sandwich truncated conical shells (STCS) with compressible or incompressible core. Impacts are assumed to occur normally over the top face-sheet and the interaction between the impactor and the structure is simulated using a new equivalent three-degree-of-freedom (TDOF) spring-mass-damper (SMD) model. The displacement fields of core and face sheets are considered by higher order and first order shear deformation theory (FSDT), respectively. Considering continuity boundary conditions between the layers, the motion equations are derived based on Hamilton's principal incorporating the curvature, in-plane stress of the core and the structural damping effects based on Kelvin-Voigt model. In order to obtain the contact force, the displacement histories and the dynamic stresses, the differential quadrature method (DQM) is used. The effects of different parameters such as number of the layers of the face sheets, boundary conditions, semi vertex angle of the cone, impact velocity of impactor, trapezoidal shape and in-plane stresses of the core are examined on the low velocity impact response of STCS. Comparison of the present results with those reported by other researchers, confirms the accuracy of the present method. Numerical results show that increasing the impact velocity of the impactor yields to increases in the maximum contact force and deflection, while the contact duration is decreased. In addition, the normal stresses induced in top layer are higher than bottom layer since the top layer is subjected to impact load. Furthermore, with considering structural damping, the contact force and dynamic deflection decrees.

Prediction of Heavy Metal Content in Compost Using Near-infrared Reflectance Spectroscopy

  • Ko, H.J.;Choi, H.L.;Park, H.S.;Lee, H.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.12
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    • pp.1736-1740
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    • 2004
  • Since the application of relatively high levels of heavy metals in the compost poses a potential hazard to plants and animals, the content of heavy metals in the compost with animal manure is important to know if it is as a fertilizer. Measurement of heavy metals content in the compost by chemical methods usually requires numerous reagents, skilled labor and expensive analytical equipment. The objective of this study, therefore, was to explore the application of near-infrared reflectance spectroscopy (NIRS), a nondestructive, cost-effective and rapid method, for the prediction of heavy metals contents in compost. One hundred and seventy two diverse compost samples were collected from forty-seven compost facilities located along the Han river in Korea, and were analyzed for Cr, As, Cd, Cu, Zn and Pb levels using inductively coupled plasma spectrometry. The samples were scanned using a Foss NIRSystem Model 6500 scanning monochromator from 400 to 2,500 nm at 2 nm intervals. The modified partial least squares (MPLS), the partial least squares (PLS) and the principal component regression (PCR) analysis were applied to develop the most reliable calibration model, between the NIR spectral data and the sample sets for calibration. The best fit calibration model for measurement of heavy metals content in compost, MPLS, was used to validate calibration equations with a similar sample set (n=30). Coefficient of simple correlation (r) and standard error of prediction (SEP) were Cr (0.82, 3.13 ppm), As (0.71, 3.74 ppm), Cd (0.76, 0.26 ppm), Cu (0.88, 26.47 ppm), Zn (0.84, 52.84 ppm) and Pb (0.60, 2.85 ppm), respectively. This study showed that NIRS is a feasible analytical method for prediction of heavy metals contents in compost.

Tetramine Analysis using Liquid Chromatography-Tandem Mass Spectrometry and Ion Chromatography (LC-MS/MS와 Ion Chromatography를 이용한 테트라민 분석)

  • Song, Ki-Cheol;Lee, Ka-Jeong;Kim, Ji-Hoe;Yoon, Ho-Dong;Yu, Hong-Sik;Mok, Jong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.1
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    • pp.45-49
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    • 2011
  • As a first step toward identifying a new method for testing sea snail tissue for toxins, and thus prevent food poisoning due to the ingestion of contaminated snails, we measured the tetramine [$(CH_3)_4N^+$] contents of sea snails from the Korean coast using both liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ion chromatography. For tetramine tested, good linearity ($r^2$ = 0.9996) was observed between the amounts in the injected samples and the peak areas of standard toxins, which ranged from 0.1 to 100 ng. The recovery (%) of tetramine from spiked tissue and mid-gut gland samples ranged from 84.0 to 95.3%. The quantitative results for tetramine using this method were in good agreement with the theoretical values. LC-MS/MS has both high sensitivity and selectivity, which makes it possible to measure trace quantities of tetramine in samples.

Diagnosis of Coronary Artery Disease in Patients with Chest Pain by Means of Magnetocardiography (흉통환자에서 심자도를 이용한 관상동맥질환의 진단)

  • Kwon, H.;Kim, K.;Kim, J.M.;Lee, Y.H.;Kim, T.E.;Lim, H.K.;Park, Y.K.;Ko, Y.G.;Chung, N.
    • Progress in Superconductivity
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    • v.8 no.1
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    • pp.46-53
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    • 2006
  • Magnetocardiography(MCG) has been proposed as a novel and non-invasive diagnostic tool for the detection of cardiac electrical abnormality associated with myocardial ischemia. In our previous study, we have proposed a new classification method of MCG parameters, based on the different populations of the parameters between coronary artery disease(CAD) patients, symptomatic patients and healthy volunteers. We used four parameters, representing the directional changes of the electrical activity in the period of an R-ST-T interval. In patients with chest pain and without ST-segment elevation, who were selected consecutively from all patients admitted to the hospital in 2004, the patients with CAD could be classified with a higher sensitivity than conventional methods, showing that the proposed method can be useful for the diagnosis of CAD with MCG. In this study, we examined the validity of the algorithm with the prior probability distribution in diagnosis of new patients admitted to the hospital in 2005. In the results, presence of CAD could be found with sensitivity and specificity of 81.3% and 71.4%, respectively, in patients with chest pain and non-diagnostic ECG findings.

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Standardization of an enzyme-linked immunosorbent assay for detection of antibody to avian reticuloendotheliosis virus (세망내피증 바이러스 항체검출을 위한 ELISA 표준화)

  • Sung, Haan Woo;Lee, Su Jeong
    • Korean Journal of Veterinary Research
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    • v.45 no.4
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    • pp.569-574
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    • 2005
  • Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to reticuloendotheliosis virus (REV) at single serum dilution was standardized. REV HI, one of the Korean field isolates, was inoculated into chicken embryo fibroblast (CEF) cells and was harvested from the culture fluids and cells after 10 to 12 days. Viruses were purified by centrifugation at the $107,000{\times}g$ for 12 hours on 20, 30, 45% (W/V) sucrose gradient. Virus specific fraction was collected and used as ELISA antigen. To standardize ELISA, the optimal concentration of coating antigen ($1{\mu}g/well$) and conjugate (1/1000) was determined by corrected OD (OD value of positive serum-OD value of negative serum) and P/N ratio (OD value of positive serum/OD value of negative serum). To calculate ELISA titer by measuring absorbance at 1/400 single serum dilution, serum titrations were carried out for various sample sera together with standard positive and negative sera. The observed titers of serum samples were plotted against sample/positive (s/p) ratios at 1/400 serum dilution. From the above data, the ELISA titers could be calculated by the equation of $log_{10}$ ELISA titer = 2.2763 ($log_{10}$ s/p) + 3.482 (r = 0.93). For evaluating the sensitivity, the standardized method were compared with conventional agar gel immunodiffusion (AGID) test method using serum samples collected from REV infected field chicken flocks. Fifty seven of 60 samples (95%) were positive for REV by ELISA, whereas only 11 (18.3%) samples were positive by AGID test. This results suggested that the ELISA tests developed in this study could be used for detection of antibodies to REV with high sensitivity.

Development of Runoff and Sediment Auto-calibration Tool for HRSM4BMP Model (HRSM4BMP 모형 유출/유사 자동 보정 툴 개발)

  • Kum, Donghyuk;Ryu, Jichul;Choi, Jaewan;Kang, Hyunwoo;Jang, Chun Hwa;Shin, Dong Suk;Lee, Jae Kwan;Lim, Kyoung Jae
    • Journal of Korean Society on Water Environment
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    • v.29 no.1
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    • pp.29-35
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    • 2013
  • Recently, various Best Management Practices (BMPs) have been applied at a field to reduce soil erosion. Hourly Runoff and Sediment Model for Best Management Practices (HRSM4BMP) model could be used to evaluate soil erosion reduction for various agricultural BMPs at fields. Runoff and sediment yield from source areas have to be predicted with greater accuracies to evaluate sediment reduction efficiently with BMPs. To achieve this, the best parameters related with runoff and sediment modules of the HRSM4BMP model should be identified with proper calibration processes. Although manual calibration is often utilized in calibrating runoff and sediment using the HRSM4BMP, objective calibration method would be recommended. The purpose of the study was to develop an automatic calibration tool of the HRSM4BMP model with PARASOL method. This automatic calibration tool was applied to Bangdongri, Chuncheon-si to evaluate its calibration performance. The $R^2$, NSE and RMSE value for runoff estimation were 0.92, 0.92, $0.3m^3$, and for sediment yield estimation were 0.94, 0.94, 0.0027 kg. As shown in this result, automatic calibration tool of HRSM4BMP model would be used to determine the best parameters and can be used to simulate runoff and sediment yield with acceptable accuracies.

Fish length dependence of acoustic target strength for 12 dominant fish species caught in the Korean waters at 75 kHz (한국 연근해에서 어획된 주요 12어종의 75 kHz에 대한 음향 반사 강도의 체장 의존성)

  • Lee, Dae-Jae
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.41 no.4
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    • pp.296-305
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    • 2005
  • Acoustic target strength (TS) of 12 commercially important fish species caught in the Korean waters had been investigated and their results were presented. Laboratory measurements of target strength on 12 dominant fish species were carried out at a frequencies of 75 kHz by single beam method under the controlled condition of the water tank with the 241 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N ($-5.0^{\circ}$, $^15.0{\circ}$). The 75 to fish length relationship for each species was independently derived by a least - squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to fish length and fish species. An empirical model for fish target strength(TS, dB) averaged over the dorsal aspect of 158 fishes of 7 species and which spans the fish length(L, m) to wavelength(${\lambda}$, m) ratio between 6.2 and 21.3 was derived: TS: 27.03 Log(L)-7.7Log(${\kanbda}$)-17.21, ($r^2$=0.59).